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KEAP1 (NM_012289) Human Untagged Clone
CAT＃: SC111946
KEAP1 (untagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2
CNY 4,656.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | INrf2; KLHL19 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_012289, the custom clone sequence may differ by one or more nucleotides
ATGCAGCCAGATCCCAGGCCTAGCGGGGCTGGGGCCTGCTGCCGATTCCTGCCCCTGCAGTCACAGTGCC CTGAGGGGGCAGGGGACGCGGTGATGTACGCCTCCACTGAGTGCAAGGCGGAGGTGACGCCCTCCCAGCA TGGCAACCGCACCTTCAGCTACACCCTGGAGGATCATACCAAGCAGGCCTTTGGCATCATGAACGAGCTG CGGCTCAGCCAGCAGCTGTGTGACGTCACACTGCAGGTCAAGTACCAGGATGCACCGGCCGCCCAGTTCA TGGCCCACAAGGTGGTGCTGGCCTCATCCAGCCCTGTCTTCAAGGCCATGTTCACCAACGGGCTGCGGGA GCAGGGCATGGAGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTCATTGAATTCGCC TACACGGCCTCCATCTCCATGGGCGAGAAGTGTGTCCTCCACGTCATGAACGGTGCTGTCATGTACCAGA TCGACAGCGTTGTCCGTGCCTGCAGTGACTTCCTGGTGCAGCAGCTGGACCCCAGCAATGCCATCGGCAT CGCCAACTTCGCTGAGCAGATTGGCTGTGTGGAGTTGCACCAGCGTGCCCGGGAGTACATCTACATGCAT TTTGGGGAGGTGGCCAAGCAAGAGGAGTTCTTCAACCTGTCCCACTGCCAACTGGTGACCCTCATCAGCC GGGACGACCTGAACGTGCGCTGCGAGTCCGAGGTCTTCCACGCCTGCATCAACTGGGTCAAGTACGACTG CGAACAGCGACGGTTCTACGTCCAGGCGCTGCTGCGGGCCGTGCGCTGCCACTCGTTGACGCCGAACTTC CTGCAGATGCAGCTGCAGAAGTGCGAGATCCTGCAGTCCGACTCCCGCTGCAAGGACTACCTGGTCAAGA TCTTCGAGGAGCTCACCCTGCACAAGCCCACGCAGGTGATGCCCTGCCGGGCGCCCAAGGTGGGCCGCCT GATCTACACCGCGGGCGGCTACTTCCGACAGTCGCTCAGCTACCTGGAGGCTTACAACCCCAGTGACGGC ACCTGGCTCCGGTTGGCGGACCTGCAGGTGCCGCGGAGCGGCCTGGCCGGCTGCGTGGTGGGCGGGCTGT TGTACGCCGTGGGCGGCAGGAACAACTCGCCCGACGGCAACACCGACTCCAGCGCCCTGGACTGTTACAA CCCCATGACCAATCAGTGGTCGCCCTGCGCCCCCATGAGCGTGCCCCGTAACCGCATCGGGGTGGGGGTC ATCGATGGCCACATCTATGCCGTCGGCGGCTCCCACGGCTGCATCCACCACAACAGTGTGGAGAGGTATG AGCCAGAGCGGGATGAGTGGCACTTGGTGGCCCCAATGCTGACACGAAGGATCGGGGTGGGCGTGGCTGT CCTCAATCGTCTCCTTTATGCCGTGGGGGGCTTTGACGGGACAAACCGCCTTAATTCAGCTGAGTGTTAC TACCCAGAGAGGAACGAGTGGCGAATGATCACAGCAATGAACACCATCCGAAGCGGGGCAGGCGTCTGCG TCCTGCACAACTGTATCTATGCTGCTGGGGGCTATGATGGTCAGGACCAGCTGAACAGCGTGGAGCGCTA CGATGTGGAAACAGAGACGTGGACTTTCGTAGCCCCCATGAAGCACCGGCGAAGTGCCCTGGGGATCACT GTCCACCAGGGGAGAATCTACGTCCTTGGAGGCTATGATGGTCACACGTTCCTGGACAGTGTGGAGTGTT ACGACCCAGATACAGACACCTGGAGCGAGGTGACCCGAATGACATCGGGCCGGAGTGGGGTGGGCGTGGC TGTCACCATGGAGCCCTGCCGGAAGCAGATTGACCAGCAGAACTGTACCTGTTGA >OriGene 5' read for NM_012289 unedited
TCACTATATTTGTATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAGCGC GGCCATGGCGGGGTCCCTGAGTGCCAGAGGTGGTGGTGTTGCTTATCTTCTGGAACCCCA TGCAGCCAGATCCCAGGCCTAGCGGGGCTGGGGCCTGCTGCCGATTCCTGCCCCTGCAGT CACAGTGCCCTGAGGGGGCAGGGGACGCGGTGATGTACGCCTCCACTGAGTGCAAGGCGG AGGTGACGCCCTCCCAGCATGGCAACCGCACCTTCAGCTACACCCTGGAGGATCATACCA AGCAGGCCTTTGGCATCATGAACGAGCTGCGGCTCAGCCAGCAGCTGTGTGACGTCACAC TGCAGGTCAAGTACCAGGATGCACCGGCCGCCCAGTTCATGGCCCACAAGGTGGTGCTGG CCTCATCCAGCCCTGTCTTCAAGGCCATGTTCACCAACGGGCTGCGGGAGCAGGGCATGG AGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTCATTGAATTCGCCT ACACGGCCTCCATCTCCATGGGCGAGAAGTGTGTCCTCCACGTCATGAACGGTGCTGTCA TGTACCAGATCGACAGCGTTGTCCGTGCCTGCAGTGACTTCCTGGTGCAGCAGCTGGACC CCAGCAATGCCATCGGCATCGCCCACTTCGCTGAGCAGATTGGCTGTGTGGAGTTGCACC ACCGTGCCCGGGGAGTACATCTACATGCATTTTGGGGAGGTTGGCCAACCAAGAGGAGTT CTTCACCCTGTCCCACTGGCCACTGGGTGACCCTCATCAGCCGGGACGACCTGNACGTGC GCTGCGAGTCCCAAGTCTTCCACGCCCTGCATCAACTGGGTGCAAGNACGACTGCGAACA GCGACCGTTTTACNTTCAGGCGCTTGCTGCGGGCCCGGCG >OriGene 3' read for NM_012289 unedited
GCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAT TAGTCTGTTCTTTATTTTCCTTGGAAAATTACAGGGTTACTTATCAACAAAATAACTGTC CCTCCGGGGGGGAAGTGCCATCCGGTGGCTTCTATGTACAGGCCCATCCCTGGGAACCAC ATTTCCAAAGAGGGGCCTCCCCCTGAGGCCCCCATTGGCCCCCTCCCAGGTATCCAAGAA TAAATCACATGGTGACAGCTGCCGGCATGGGTGAGTGGGACCCAAGCCTATTACCCGGCC AGAGGGTTTGGGGGCCTCTCTCCTGGAAGCCTGCTCTTTCCACACCCCCTTTCCCAGCCA GGCTGTCTTGGACACTCCCGGGGCTCCGCTGAGGGGCACATGATTCCCGCTTTGGACTTC TTTTGAGATGTCATTTTAACACTGAGGCATCCTGGCCTCCCTTCCCGGAAAATGGGTTAT TTGCAGAGCTGGCTTTTCTTTTAATCCCGGTTTTTGTACAAAAACAATGATACTCCCCAT TGGACTGTATTTTTGCCCAAAAAACAAAAGTGCCTCAACAGGTACAGTTCTGCTGGGCAA TCTGCTTCCCGGAGGGCTCCATGGTGACAGCCACCCCCACCCCACTCCCGCCCCGATGGC TTTCCGGTCACCTCGCCCCAAGTGGCTTGATTCTGGGCCGAACCCTCCACACTGGTCCGG AACCTGTGACCCTCTTAGCCCCAAGGCCTAAATTCTCCCCTGGGGCCAAGGATCCCCAGG CCCTTTCCGGGCCTTTGGGGCCTCCAAACTCCCGTCTCTTTTCACCACAAACGCCCCCCC TGTCACCGGGCCCGCCCCCATCCCCCCCCCCTAATACCCCCGGACAGACCAAACCCCTCC CCTTCCGGAGGGCCCCTGCTCTACCCCCGCACCCCCCCTTTGGGGTAGAACCCCACGAAT ACGCCGTCGCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012289 |
| Insert Size | 2600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012289.3, NP_036421.2 |
| RefSeq Size | 2577 bp |
| RefSeq ORF | 1875 bp |
| Locus ID | 9817 |
| UniProt ID | Q14145 |
| Domains | BTB, Kelch |
| Protein Families | Transcription Factors |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) has an alternate 5' UTR, as compared to variant 1. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Rotavirus NSP1 associates with components of the cullin RING ligase family of E3 ubiquitin ligases
,Lutz, LM;Pace, CR;Arnold, MM;,
J. Virol.
,PubMed ID 27099313
[KEAP1]
|
|
KEAP1 Is a Redox Sensitive Target That Arbitrates the Opposing Radiosensitive Effects of Parthenolide in Normal and Cancer Cells
,Yong Xu, Fang Fang, Sumitra Miriyala, Peter A. Crooks, Terry D. Oberley, Luksana Chaiswing, Teresa Noel, Aaron K. Holley, Yanming Zhao, Kelley K. Kiningham, Daret K. St. Clair, and William H. St. Clair,
Cancer Res., Jul 2013; 73: 4406 - 4417.
,PubMed ID 23674500
[KEAP1]
|
|
Dysfunctional KEAP1–NRF2 Interaction in Non-Small-Cell Lung Cancer
,Singh A, Misra V, Thimmulappa RK, Lee H, Ames S, et al.,
PLoS Med 3(10): e420. doi:10.1371/journal.pmed.0030420
[KEAP1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202513 | KEAP1 (Myc-DDK-tagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2 |
CNY 4,648.00 |
|
| RC202513L1 | Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, Myc-DDK-tagged |
CNY 7,048.00 |
|
| RC202513L2 | Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC202513L3 | Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, Myc-DDK-tagged |
CNY 7,048.00 |
|
| RC202513L4 | Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, mGFP tagged |
CNY 7,048.00 |
|
| RG202513 | KEAP1 (tGFP-tagged) - Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2 |
CNY 6,248.00 |
