DENND1A (NM_024820) Human Untagged Clone

CAT#: SC111995

DENND1A (untagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 2



  "NM_024820" in other vectors (6)

CNY 4,168.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Cited in 3 publications.

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DENND1A rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms FAM31A; KIAA1608
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_024820 edited
ATGGGCTCCAGGATCAAGCAGAATCCAGAGACCACATTTGAAGTATATGTTGAAGTGGCC
TATCCCAGGACAGGTGGCACTCTTTCAGATCCTGAGGTGCAGAGGCAATTCCCGGAGGAC
TACAGTGACCAGGAAGTTCTACAGACTTTGACCAAGTTTTGTTTCCCCTTCTATGTGGAC
AGCCTCACAGTTAGCCAAGTTGGCCAGAACTTCACATTCGTGCTCACTGACATTGACAGC
AAACAGAGATTCGGGTTCTGCCGCTTATCTTCAGGAGCGAAGAGCTGCTTCTGTATCTTA
AGCT:ATCTCCCCTGGTTCGAGGTATTTTATAAGCTGCTTAACATCCTGGCAGATTACAC
GACAAAAAGACAGGAAAATCAGTGGAATGAGCTTCTTGAAACTCTGCACAAACTTCCCAT
CCCTGACCCAGGAGTGTCTGTCCATCTCAGCGTGCATTCTTATTTTACTGTGCCTGATAC
CAGAGAACTTCCCAGCATACCTGAGAATAGAAATCTGACAGAATATTTTGTGGCTGTGGA
TGTTAACAACATGTTGCATCTGTACGCCAGTATGCTGTACGAACGCCGGATACTCATCAT
TTGCAGCAAACTCAGCACTCTGACTGCCTGCATCCACGGGTCTGCGGCGATGCTCTACCC
CATGTACTGGCAGCACGTGTACATCCCCGTGCTGCCGCCGCATCTGCTGGACTACTGCTG
TGCTCCCATGCCCTACCTCATAGGAATCCATTTAAGTTTAATGGAGAAAGTCAGAAACAT
GGCCCTGGATGATGTCGTGATCCTGAATGTGGACACCAACACCCTGGAAACCCCCTTCGA
TGACCTCCAGAGCCTCCCAAACGACGTGATCTCTTCCCTGAAGAACAGGCTGAAAAAGGT
CTCCACAACCACTGGGGATGGTGTGGCCAGAGCGTTCCTCAAGGCCCAGGCTGCTTTCTT
CGGTAGCTACCGAAACGCTCTGAAAATCGAGCCGGAGGAGCCGATCACTTTCTGTGAGGA
AGCCTTCGTGTCCCACTACCGCTCCGGAGCCATGAGGCAGTTCCTGCAGAACGCCACACA
GCTGCAGCTCTTCAAGCAGTTTATTGATGGTCGATTAGATCTTCTCAATTCCGGCGAAGG
TTTCAGTGATGTTTTTGAAGAGGAAATCAACATGGGCGAGTACGCTGGCAGTGACAAACT
GTACCATCAGTGGCTCTCCACTGTCCGGAAAGGAAGTGGAGCAATTCTGAATACTGTAAA
GACCAAAGCAAATCCGGCCATGAAGACTGTCTACAAGTTCGCAAAAGATCATGCAAAAAT
GGGAATAAAAGAGGTGAAAAACCGCTTGAAGCAAAAGGACATTGCCGAGAATGGCTGCGC
CCCCACCCCAGAAGAGCAGCTGCCAAAGACTGCACCGTCCCCACTGGTGGAGGCCAAGGA
CCCCAAGCTCCGAGAAGACCGGCGGCCAATCACAGTCCACTTTGGACAGGTGCGCCCACC
TCGTCCACATGTTGTTAAGAGACCAAAGAGCAACATCGCAGTGGAAGGCCGGAGGACGTC
TGTGCCGAGCCCTGAGCAAAACACCATTGCAACACCAGCTACACTCCACATCCTACAGAA
AAGCATTACCCATTTTGCGGCCAAGTTCCCGACGAGAGGCTGGACCTCTTCATCACATTG
A
Restriction Sites NotI-NotI     
ACCN NM_024820
Insert Size 1700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_024820.2.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_024820.2, NP_079096.2
RefSeq Size 2166 bp
RefSeq ORF 1680 bp
Locus ID 57706
UniProt ID Q8TEH3
Domains DENN, dDENN, uDENN
Gene Summary Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]
Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1, that causes a frameshift. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (3)

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