LEPRE1 (P3H1) (NM_022356) Human Untagged Clone

CAT#: SC112534

LEPRE1 (untagged)-Human leucine proline-enriched proteoglycan (leprecan) 1 (LEPRE1), transcript variant 1



  "NM_022356" in other vectors (6)

CNY 5,896.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
LEPRE1 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GROS1; LEPRE1; OI8
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_022356 edited
ATGGCGGTACGCGCGTTGAAGCTGCTGACCACACTGCTGGCTGTCGTGGCCGCTGCCTCC
CAAGCCGAGGTCGAGTCCGAGGCAGGATGGGGCATGGTGACGCCTGATCTGCTCTTCGCC
GAGGGGACCGCAGCCTACGCGCGCGGGGACTGGCCCGGGGTGGTCCTGAGCATGGAACGG
GCGCTGCGCTCCCGGGCAGCCCTCCGCGCCCTTCGCCTGCGCTGCCGCACCCAGTGTGCC
GCCGACTTCCCGTGGGAGCTGGACCCCGACTGGTCCCCCAGCCCGGCCCAGGCCTCGGGC
GCCGCCGCCCTGCGCGACCTGAGCTTCTTCGGGGGCCTTCTGCGTCGCGCTGCCTGCCTG
CGCCGCTGCCTCGGGCCGCCGGCCGCCCACTCGCTCAGCGAAGAGATGGAGCTGGAGTTC
CGCAAGCGGAGCCCCTACAACTACCTGCAGGTCGCCTACTTCAAGATCAACAAGTTGGAG
AAAGCTGTTGCTGCAGCACACACCTTCTTCGTGGGCAATCCTGAGCACATGGAAATGCAG
CAGAACCTAGACTATTACCAAACCATGTCTGGAGTGAAGGAGGCCGACTTCAAGGATCTT
GAGACTCAACCCCATATGCAAGAATTTCGACTGGGAGTGCGACTCTACTCAGAGGAACAG
CCACAGGAAGCTGTGCCCCACCTAGAGGCGGCGCTGCAAGAATACTTTGTGGCCTATGAG
GAGTGCCGTGCCCTCTGCGAAGGGCCCTATGACTACGATGGCTACAACTACCTTGAGTAC
AACGCTGACCTCTTCCAGGCCATCACAGATCATTACATCCAGGTCCTCAACTGTAAGCAG
AACTGTGTCACGGAGCTTGCTTCCCACCCAAGTCGAGAGAAGCCCTTTGAAGACTTCCTC
CCATCGCATTATAATTATCTGCAGTTTGCCTACTATAACATTGGGAATTATACACAGGCT
GTTGAATGTGCCAAGACCTATCTTCTCTTCTTCCCCAATGACGAGGTGATGAACCAAAAT
TTGGCCTATTATGCAGCTATGCTTGGAGAAGAACACACCAGATCCATCGGCCCCCGTGAG
AGTGCCAAGGAGTACCGACAGCGAAGCCTACTGGAAAAAGAACTGCTTTTCTTCGCTTAT
GATGTTTTTGGAATTCCCTTTGTGGATCCGGATTCATGGACTCCAGAAGAAGTGATTCCC
AAGAGATTGCAAGAGAAACAGAAGTCAGAACGGGAAACAGCCGTACGCATCTCCCAGGAG
ATTGGGAACCTTATGAAGGAAATCGAGACCCTTGTGGAAGAGAAGACCAAGGAGTCACTG
GATGTGAGCAGACTGACCCGGGAAGGTGGCCCCCTGCTGTATGAAGGCATCAGTCTCACC
ATGAACTCCAAACTCCTGAATGGTTCCCAGCGGGTGGTGATGGACGGCGTAATCTCTGAC
CACGAGTGTCAGGAGCTGCAGAGACTGACCAATGTGGCAGCAACCTCAGGAGATGGCTAC
CGGGGTCAGACCTCCCCACATACTCCCAATGAAAAGTTCTATGGTGTCACTGTCTTCAAA
GCCCTCAAGCTGGGGCAAGAAGGCAAAGTTCCTCTGCAGAGTGCCCACCTGTACTACAAC
GTGACGGAGAAGGTGCGGCGCATCATAGAGTCCTACTTCCGCCTGGATACGCCCCTCTAC
TTTTCCTACTCTCATCTGGTGTGCCGCACTGCCATCGAAGAGGTCCAGGCAGAGAGGAAG
GATGATAGTCATCCAGTCCACGTGGACAACTGCATCCTGAATGCCGAGACCCTCGTGTGT
GTCAAAGAGCCCCCAGCCTACACCTTCCGCGACTACAGCGCCATCCTTTACCTAAATGGG
GACTTCGATGGCGGAAACTTTTATTTCACTGAACTGGATGCCAAGACCGTGACGGCAGAG
GTGCAGCCTCAGTGTGGAAGAGCCGTGGGATTCTCTTCAGGCACTGAAAACCCACATGGA
GTGAAGGCTGTCACCAGGGGGCAGCGCTGTGCCATCGCCCTGTGGTTCACCCTGGACCCT
CGACACAGCGAGCGGGACAGGGTGCAGGCAGATGACCTGGTGAAGATGCTCTTCAGCCCA
GAAGAGATGGACCTCTCCCAGGAGCAGCCCCTGGATGCCCAGCAGGGCCCCCCCGAACCT
GCACAAGAGTCTCTCTCAGGCAGTGAATCGAAGCCCAAGGATGAGCTATGA
>OriGene 5' read for NM_022356 unedited
GGTTCGAAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTGG
CTGGCGGTTCCGTTAGGTCTGAGGGAGCGATGGCGGTACGCGCGTTGAAGCTGCTGACCA
CACTGCTGGCTGTCGTGGCCGCTGCCTCCCAAGCCGAGGTCGAGTCCGAGGCAGGATGGG
GCATGGTGACGCCTGATCTGCTCTTCGCCGAGGGGACCGCAGCCTACGCGCGCGGGGACT
GGCCCGGGGTGGTCCTGAGCATGGAACGGGCGCTGCGCTCCCGGGCAGCCCTCCGCGCCC
TTCGCCTGCGCTGCCGCACCCAGTGTGCCGCCGACTTCCCGTGGGAGCTGGACCCCGACT
GGTCCCCCAGCCCGGCCCAGGCCTCGGGCGCCGCCGCCCTGCGCGACCTGAGCTTCTTCG
GGGGCCTTCTGCGTCGCGCTGCCTGCCTGCGCCGCTGCCTCGGGCCGCCGGCCGCCCACT
CGCTCAGCGAAGAGATGGAGCTGGAGTTCCGCAAGCGGAGCCCCTACAACTACCTGCAGG
TCGCCTACTTCAAGATCAACAAGTTGGAGAAAGCTGTTGCTGCAGCACACACCTTCTTCG
TGGGCAATCCTGAGCACATGGAAATGCAGCAGAACCTAGACTATTACCAAACCATGTCTG
GAGTGAAGGAGGCCGACTTCAAGGATCTTGAGACTCAACCCCATATGCAAGAATTTCGAC
TGGGAGTGCGACTCTACTCAGAGGAACAGCCACCAGAAGCTGTGCCCCACCTAGAGGCCG
CGCTGCAAGAATACTTTGTGGCCTATGAGGAGTGCCGTGCCCTCTGCGAAAGCCCCTATG
ACTACGATGGCTACCACTACCTTGGATACAACGCTGACCTCTTCCAGGCCATCACAGATC
ATTACATCCAAGTTCTCAC
>OriGene 3' read for NM_022356 unedited
NTTTACCTGGATNATTTATTCTTTGGTTGTCTACACAGACACTTAAGTACTGTATCGCTG
TTATGCAGCGGCCTGTGGAGGCCCCTGGGGGTGGCTGGGCCTGTGTCCTGAGCCCTCAGC
CAGATCCAGGGGGTGCGGTGTCTGGTCATGTCCACTCCAAGAGCAGTAGCACCATGTAGA
AGGCTGTGAGCAGGGTCCCCTCGGCTGACTGGCAGATGTAGGCTCACTGCTCTGCAGCCC
CGAGGGGCTGGCCAGCTCAGAGTGCAGAAGAGTTCCTCTCCATGGGTCTAGTCACCCATC
CGTCTGACCTGGACGCTGTCATAGCTCATCCTTGGGCTTCGATTCACTGGCTGAGAGAGA
CTGTTGTGCAGGTACTGGGGGCCCCTGCTGGGCATCCAGGGGCTGCTCCTGCGATAGGTG
CATAAATTGTGGGCTGTGCACCATCTTCACCGGCTCATATAGCTGCTCCCTGTATCACTC
CTTGGGCTAAGGTATCCACCTAAACACCACGACGCTGGTGCGACATTGTCTGTGCTGTCA
ATATAATCAAATTAATGCATCCATTGTTAGATTTAAGATTTATCTCTTTTTTTCGTCCGT
CTTATCTGTACTTTTATCTTTTCTTCTGTCTCTTCCCTTTCCTCGATCCCCCCCCTAGTC
CCCTCTTCCTCCTTCCTCCTTATATTACTCCCCTCCCGCTCCTTCGCACTTTCCAACACC
TCGGCTTCGATAATACTCCGTTCTCTCCTCCTTCGTCCTCTCCCTCACCCACCGCATATC
CACCCGATTCCCCCCTTCCGCGTTTCCCGCCTGCTCTCCTTTCCCCTCTCTCCTTATTCT
CCCTCCCTCTCTCGCGGCTTCCCCTCCCTGTTTCTCTTTTTTCCTCGCCCTCTTCTCCCT
CCTCGCTTTCTGTATCTCTCTCCCCCTCTCTCCTTCTCCGTCTG
Restriction Sites NotI-NotI     
ACCN NM_022356
Insert Size 2900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022356.2, NP_071751.2
RefSeq Size 2993 bp
RefSeq ORF 2415 bp
Locus ID 64175
UniProt ID Q32P28
Domains 2OG-FeII_Oxy, P4Hc
Protein Families Secreted Protein
Gene Summary This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq, Aug 2011]
Transcript Variant: This variant (1) differs in the 3' UTR and coding sequence compared to variant 3. The resulting isoform (1) has a shorter and distinct C-terminus compared to isoform 3.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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