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DNA polymerase delta p50 (POLD2) (NM_006230) Human Untagged Clone
CAT＃: SC112691
POLD2 (untagged)-Human polymerase (DNA directed), delta 2, regulatory subunit 50kDa (POLD2), transcript variant 2
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112691 sequence for NM_006230 edited (data generated by NextGen Sequencing)
ATGTTTTCTGAGCAGGCTGCCCAGAGGGCCCACACTCTACTGTCCCCACCATCAGCCAAC AATGCCACCTTTGCCCGGGTGCCAGTGGCAACCTACACCAACTCCTCACAACCCTTCCGG CTAGGAGAGCGCAGCTTTAGCCGGCAGTATGCCCACATTTATGCCACCCGCCTCATCCAA ATGAGACCCTTCCTGGAGAACCGGGCCCAGCAGCACTGGGGCAGTGGAGTGGGAGTGAAG AAGCTGTGTGAACTGCAGCCTGAGGAGAAGTGCTGTGTGGTGGGCACTCTGTTCAAGGCC ATGCCGCTGCAGCCCTCCATCCTGCGGGAGGTCAGCGAGGAGCACAACCTGCTCCCCCAG CCTCCTCGGAGTAAATACATACACCCAGATGACGAGCTGGTCTTGGAAGATGAACTGCAG CGTATCAAACTAAAAGGCACCATTGACGTGTCAAAGCTGGTTACGGGGACTGTCCTGGCT GTGTTTGGCTCCGTGAGAGACGACGGGAAGTTTCTGGTGGAGGACTATTGCTTTGCTGAC CTTGCTCCCCAGAAGCCCGCACCCCCACTTGACACAGATAGGTTTGTGCTACTGGTGTCC GGCCTGGGCCTGGGTGGCGGTGGAGGCGAGAGCCTGCTGGGCACCCAGCTGCTGGTGGAT GTGGTGACGGGGCAGCTTGGGGACGAAGGGGAGCAGTGCAGCGCCGCCCACGTCTCCCGG GTTATCCTCGCTGGCAACCTCCTCAGCCACAGCACCCAGAGCAGGGATTCTATCAATAAG GCCAAATACCTCACCAAGAAAACCCAGGCAGCCAGCGTGGAGGCTGTTAAGATGCTGGAT GAGATCCTCCTGCAGCTGAGCGCCTCAGTGCCCGTGGACGTGATGCCAGGCGAGTTTGAT CCCACCAATTACACGCTCCCCCAGCAGCCCCTCCACCCCTGCATGTTCCCGCTGGCCACT GCCTACTCCACGCTCCAGCTGGTCACCAACCCCTACCAGGCCACCATTGATGGAGTCAGA TTTTTGGGGACATCAGGACAGAACGTGAGTGACATTTTCCGATACAGCAGCATGGAGGAT CACTTGGAGATCCTGGAGTGGACCCTGCGGGTCCGTCACATCAGCCCCACAGCCCCTGAC ACTCTAGGTTGTTACCCCTTCTACAAAACTGACCCGTTCATCTTCCCAGAGTGCCCGCAT GTCTACTTTTGTGGCAACACCCCCAGCTTTGGCTCCAAAATCATCCGAGGTCCTGAGGAC CAGACAGTGCTGTTGGTGACTGTCCCTGACTTCAGTGCCACGCAGACCGCCTGCCTTGTG AACCTGCGCAGCCTGGCCTGCCAGCCCATCAGCTTCTCGGGCTTCGGGGCAGAGGACGAT GACCTGGGAGGCCTGGGGCTGGGCCCCTGA Clone variation with respect to NM_006230.2 >OriGene 5' read for NM_006230 unedited
TTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCGGGATGCGGCGCGCCGC GCGTGAACCTCCTTGGCCTGGGCGAAGCTGTGTGGACCAAGCAAGTCAGGAGTGTGGCCA TGTTTTCTGAGCAGGCTGCCCAGAGGGCCCACACTCTACTGTCCCCACCATCAGCCAACA ATGCCACCTTTGCCCGGGTGCCAGTGGCAACCTACACCAACTCCTCACAACCCTTCCGGC TAGGAGAGCGCAGCTTTAGCCGGCAGTATGCCCACATTTATGCCACCCGCCTCATCCAAA TGAGACCCTTCCTGGAGAACCGGGCCCAGCAGCACTGGGGCAGTGGAGTGGGAGTGAAGA AGCTGTGTGAACTGCAGCCTGAGGAGAAGTGCTGTGTGGTGGGCACTCTGTTCAAGGCCA TGCCGCTGCAGCCCTCCATCCTGCGGGAGGTCAGCGAGGAGCACAACCTGCTCCCCCAGC CTCCTCGGAGTAAATACATACACCCAGATGACGAGCTGGTCTTGGAAGATGAACTGCAGC GTATCAAACTAAAAGGCACCATTGACGTGTCAAAGCTGGTTACGGNGACTGTCCTGGCTG TGTTTGGCTCCGTGAGAGACGACGGGAAGTTTCTGGTGGAGGACTATTGCTTTGCTGACC TTGCTCCCANAAGCCCGCACCCNCACTTGACACAGATAGGTTTGTGCTACTGGTGTCCGG CCTGGGCCTNGGTGGGCGGTGGAGCAAAAGCCTGCTGGGACCCANCTGCTGTTGGATGTG GTGACGGGGCATCTGGGGGACGAAGGGGACAGTGCACGCCCGCCACGTCTCCGGGTATCC TCGTTGGCACCTCCTCACCACAGCACCAAAGAAGGGATCTATCAATAGGCCAATACCTAA CAAAAAACCCAGCAGCAGGGGGAG >OriGene 3' read for NM_006230 unedited
TGGACCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCGCATCAGCAAGT GCTCAGGCTTTATTTACAATGCCGACACTGCAGGGAATGAACAGCCTCCATCTGGGCCTC TCTGGTCAAAACCACTTTTTTGAGTCAGGGGCCCAGCCCCAGGCCTCCCAGGTCATCGTC CTCTGCCCCGAAGCCCGAGAAGCTGATGGGCTGGCAGGCCAGGCTGCGCAGGTTCACAAG GCAGGCGGTCTGCGTGGCACTGAAGTCAGGGACAGTCACCAACAGCACTGTCTGGTCCTC AGGACCTCGGATGATTTTGGAGCCAAAGCTGGGGGTGTTGCCACAAAAGTAGACATGCGG GCACTCTGGGAAGATGAACGGGTCAGTTTTGTAGAAGGGGTAACAACCTATAGTGTCAGG GGCTGTGGGGCTGATGTGACGGACCCGCAGGGTCCACTCCAGGATCTCCAAGTGATCCTC CATGCTGCTGTATCGGAAAATGTCACTCACGTTCTGTCCTGATGTCCCCAAAAATCTGAC TCCATCAATGGTGGCCTGGTAGGGGTTTGGTGACCACCTGGAGCGTGGAGTTTGCTATTG CTCACTGGTAACATGCAAGGGCTGGAGGCCTTGCTGGGGGACCGTTTTATTGGTGGTCCG AACCAGCTGTGCCGTGACGGCCCACCTACATTGGGCTACTTACCATCGCTCGCCCCCCTT TGCTCCCGTCCCCATATCCCACTCACTCTTCCGACTTGAACGGACAGCTCCCCCGCCCCG CGTGTCGTCAATAAATGTGATGCCCCTACCGCGCAGACCGCTTGTTTTTTTCACCTATAA CCCTAGTTATAGCGTATCACGCTCTCCGAATCCCACTGTGTCTTTTCTTCTTCTTTTGTG TATTCGTCCCCGCGCCCACTGCCCGCCCCCTCTCGCCCTCCCCAACTGTTTCTCCGGTTG TCTTTCTGACCCCCCGCGACTCTTCTCTGTCCCGATTAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006230 |
| Insert Size | 1510 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006230.1, NP_006221.1 |
| RefSeq Size | 1584 bp |
| RefSeq ORF | 1410 bp |
| Locus ID | 5425 |
| UniProt ID | P49005 |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Base excision repair, DNA replication, Homologous recombination, Metabolic pathways, Mismatch repair, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism |
| Gene Summary | This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at an upstream start codon, compared to variant 1. The encoded isoform (2) has a longer N-terminus, compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201793 | POLD2 (Myc-DDK-tagged)-Human polymerase (DNA directed), delta 2, regulatory subunit 50kDa (POLD2), transcript variant 2 |
CNY 3,656.00 |
|
| RC201793L3 | Lenti ORF clone of Human polymerase (DNA directed), delta 2, regulatory subunit 50kDa (POLD2), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC201793L4 | Lenti ORF clone of Human polymerase (DNA directed), delta 2, regulatory subunit 50kDa (POLD2), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG201793 | POLD2 (tGFP-tagged) - Human polymerase (DNA directed), delta 2, regulatory subunit 50kDa (POLD2), transcript variant 2 |
CNY 5,256.00 |
|
| SC320576 | POLD2 (untagged)-Human polymerase (DNA directed), delta 2, regulatory subunit 50kDa (POLD2), transcript variant 2 |
CNY 3,656.00 |
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