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SLC5A7 (NM_021815) Human Untagged Clone
CAT＃: SC112868
SLC5A7 (untagged)-Human solute carrier family 5 (choline transporter), member 7 (SLC5A7)
CNY 6,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CHT; CHT1; CMS20; HMN7A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112868 sequence for NM_021815 edited (data generated by NextGen Sequencing)
ATGGCTTTCCATGTGGAAGGACTGATAGCTATCATCGTGTTCTACCTTCTAATTTTGCTG GTTGGAATATGGGCTGCCTGGAGAACCAAAAACAGTGGCAGCGCAGAAGAGCGCAGCGAA GCCATCATAGTTGGTGGCCGAGATATTGGTTTATTGGTTGGTGGATTTACCATGACAGCT ACCTGGGTCGGAGGAGGGTATATCAATGGCACAGCTGAAGCAGTTTATGTACCAGGTTAT GGCCTAGCTTGGGCTCAGGCACCAATTGGATATTCTCTTAGTCTGATTTTAGGTGGCCTG TTCTTTGCAAAACCTATGCGTTCAAAGGGGTATGTGACCATGTTAGACCCGTTTCAGCAA ATCTATGGAAAACGCATGGGCGGACTCCTGTTTATTCCTGCACTGATGGGAGAAATGTTC TGGGCTGCAGCAATTTTCTCTGCTTTGGGAGCCACCATCAGCGTGATCATCGATGTGGAT ATGCACATTTCTGTCATCATCTCTGCACTCATTGCCACTCTGTACACACTGGTGGGAGGG CTCTATTCTGTGGCCTACACTGATGTCGTTCAGCTCTTTTGCATTTTTGTAGGGCTGTGG ATCAGCGTCCCCTTTGCATTGTCACATCCTGCAGTCGCAGACATCGGGTTCACTGCTGTG CATGCCAAATACCAAAAGCCGTGGCTGGGAACTGTTGACTCATCTGAAGTCTACTCTTGG CTTGATAGTTTTCTGTTGTTGATGCTGGGTGGAATCCCATGGCAAGCATACTTTCAGAGG GTTCTCTCTTCTTCCTCAGCCACCTATGCTCAAGTGCTGTCCTTCCTGGCAGCTTTCGGG TGCCTGGTGATGGCCATCCCAGCCATACTCATTGGGGCCATTGGAGCATCAACAGACTGG AACCAGACTGCATATGGGCTTCCAGATCCCAAGACTACAGAAGAGGCAGACATGATTTTA CCAATTGTTCTGCAGTATCTCTGCCCTGTGTATATTTCTTTCTTTGGTCTTGGTGCAGTT TCTGCTGCTGTTATGTCATCAGCAGATTCTTCCATCTTGTCAGCAAGTTCCATGTTTGCA CGGAACATCTACCAGCTTTCCTTCAGACAAAATGCTTCGGACAAAGAAATCGTTTGGGTT ATGCGAATCACAGTGTTTGTGTTTGGAGCATCTGCAACAGCCATGGCCTTGCTGACGAAA ACTGTGTATGGGCTCTGGTACCTCAGTTCTGACCTTGTTTACATCGTTATCTTCCCCCAG CTGCTTTGTGTACTCTTTGTTAAGGGAACCAACACCTATGGGGCCGTGGCAGGTTATGTT TCTGGCCTCTTCCTGAGAATAACTGGAGGGGAGCCATATCTGTATCTTCAGCCCTTGATC TTCTACCCTGGCTATTACCCTGATGATAATGGTATATATAATCAGAAATTTCCATTTAAA ACACTTGCCATGGTTACATCATTCTTAACCAACATTTGCATCTCCTATCTAGCCAAGTAT CTATTTGAAAGTGGAACCTTGCCACCTAAATTAGATGTATTTGATGCTGTTGTTGCAAGA CACAGTGAAGAAAACATGGATAAGACAATTCTTGTCAAAAATGAAAATATTAAATTAGAT GAACTTGCACTTGTGAAGCCACGACAGAGCATGACCCTCAGCTCAACTTTCACCAATAAA GAGGCCTTCCTTGATGTTGATTCCAGTCCAGAAGGGTCTGGGACTGAAGATAATTTACAG TGA Clone variation with respect to NM_021815.2 >OriGene 5' read for NM_021815 unedited
GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTCGCGTGCAGCCACC ACTCCAGAAGACTTAATGAAGTAGCCAGCTGCAGAAGAATCTGGATCATTAGATAAAAAT GGCTTTCCATGTGGAAGGACTGATAGCTATCATCGTGTTCTACCTTCTAATTTTGCTGGT TGGAATATGGGCTGCCTGGAGAACCAAAAACAGTGGCAGCGCAGAAGAGCGCAGCGAAGC CATCATAGTTGGTGGCCGAGATATTGGTTTATTGGTTGGTGGATTTACCATGACAGCTAC CTGGGTCGGAGGAGGGTATATCAATGGCACAGCTGAAGCAGTTTATGTACCAGGTTATGG CCTAGCTTGGGCTCAGGCACCAATTGGATATTCTCTTAGTCTGATTTTAGGTGGCCTGTT CTTTGCAAAACCTATGCGTTCAAAGGGGTATGTGACCATGTTAGACCCGTTTCAGCAAAT CTATGGAAAACGCATGGGCGGACTCCTGTTTATTCCTGCACTGATGGGAGAAATGTTCTG GGCTGCAGCAATTTTCTCTGCTTTGGGAGCCACCATCAGCGTGATCATCGATGTGGATAT GCACATTTCTGTCATCATCTCTGCACTCATTGCCACTCTGTACACACTGGTGNGGAGGGC TCTATTCTGTGGCCTACACTGATGTCGTTCAGCTCTTTTGCATTTTTGTAGGGCTGTGGA TCAGCGTNCCCTTTGCATGTCACATCCTGCAGTCGAGACATCGGGTTCACTGCTGTGCAT GCCAATACCAAAGCCGTGCTGGNGAACTGTGACTCTCTGAAGNCTACTCTGCTTGAAAGN TTTCTGTGGTGAAGCTGGGGGGAATCCTGCAAGCTACTTTTTAAAGGTTTTTTTTTTTCT CAGCACTAAG >OriGene 3' read for NM_021815 unedited
TCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT AATTNAAAGCATAATTTTATATAATAATTTGACAAACAAAAATATTTGTTTTAAAACCAT TATTTGCCTTTGTCTTTGAAGTACCAAACGTCCAATAAATTCCCCCTCTTCCCCGGGTAA ATTTACCTGAAGGCTAAACCAAAAAAAAAAATCTTAATTTATGTTAAAAATATAAGGGTA ATTTATTATTCCCAAGTAAAAAAAGGAATACCCCTTTTATTAAATTCACTTATTTTAAGC TTATATTCGTTTCATAGGCACATAAAATTCATTACTTGTAAGGGTACCAATGAAGTTGTA TTTTGATGCTGTTTGAAACAACTTGTTGTTGATATTGCAGTCCACCTACAAGCTTGCTGA TTTGAATATGTGGGGGAAAACAAACCTTTTAAATTCCTCAAGAAATATGTTCCATTCTTA TCAAAAGTTTGAAAATAAAATATAGGTTTTCTTCTAATGTTTTATCCACAGGTGTTGCCT TCCCAAATAAAAAAGCCATTGGCAAAAATGTTCCGTTATTTTTTTNTTCTTCTGAGGGCA AATTTTGAAAATCAGGCTTACTGGATTGAACCTGGAAAAACCTGTAAATGTCTGCTTTAC TTATTTTTGTAAACCACAGCCATTGAGGGGCCAAAGTATCCTTTTCGCCATTTAACAACT TTGGGAACACCTGTTTGTGAAAATGGGGTGAAATCTATGCCCAAGTGGCTAAGTAAAACC TTGGGCCATTGTTTCCTCCTTGCATTTTAAATTTTTGACTTAAAAACATTTGCCAACTTA ACCTTTTGGGGATAAAACAACTACCTTTGTTACCACTTTGGGGGCGGGAGGAAAAGGGGC AACACCCAAATTTTTTTGGGTCAAATTTAAGTTTAGTTTAGCCTTGAACGGAAATTTTCC TGTTTTGTTTGTGAAATGATACCGGAAAATTTCTGGAACTTTTTCCCTGAATG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021815 |
| Insert Size | 5310 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021815.2, NP_068587.1 |
| RefSeq Size | 5158 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 60482 |
| UniProt ID | Q9GZV3 |
| Domains | SSF |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224138 | SLC5A7 (Myc-DDK-tagged)-Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) |
CNY 6,480.00 |
|
| RC224138L1 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), Myc-DDK-tagged |
CNY 8,880.00 |
|
| RC224138L2 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), mGFP tagged |
CNY 8,880.00 |
|
| RC224138L3 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC224138L4 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), mGFP tagged |
CNY 6,460.00 |
|
| RG224138 | SLC5A7 (tGFP-tagged) - Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) |
CNY 8,080.00 |
