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NFYA (NM_021705) Human Untagged Clone
CAT＃: SC112917
NFYA (untagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 2
CNY 2,400.00
CNY 2,950.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CBF-A; CBF-B; HAP2; NF-YA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_021705, the custom clone sequence may differ by one or more nucleotides
ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGC AGCAGGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACC CATCATGGTCCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAG GGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTGCAGGTGC AGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCTGTCACTGCTGGCCAGAC TCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTC TATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTA TCCCAGCAGCCAGTTTGGCAGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGG GCAAGGGACTGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGATG GTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGATGCTTGAAGAAG AGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAGGCAAGCCCGAGCTAAACTAGA GGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCTGCATGAGTCTCGGCACCGTCATGCCATGGCA CGGAAGCGTGGTGAAGGTGGACGATTTTTCTCTCCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAA ACCAAGCCGATGAAGAAGCAATGACACAGATCATCCGAGTGTCCTAA >OriGene 5' read for NM_021705 unedited
NGGGTTTCANATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTAGG ATCTCCAGAGTGGACAGGAATCTCACTTGGAGGGACCATGGAGCAGTATACAGCAAACAG CAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGCAGCAGGTCCAAGG GCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACCCAT CATGGTCCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGG AACACAGGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGG ACAGGCTGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCC CCAGACGGCTGTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACA GCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGG CACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGCCAG TTTGGCAGGAGCACAGATTGTTCANACAGGAGCCAATACCAACACAACCAGCAGTGGGCA AGGGACTGTCACTGTGACACTACCAGTGGCANGGCATGTGGTCAATTCAGGAAGGATGGT CATGATGGTTCCTGGGGCTGCCTCTGTGCCTGCTANCCAAAGATCCCTCTACTGGAGCAN AGATGCTGAAGAAGACCTCTTACGTGAATGCCAACATACCACGTATTCTTAAGAGGAGCA GCCCGAGCTAACTAGAGCAGAGGGGAAATTCAAGGAGAGAAGAATACTGCTGAGTCTCGC ACGTCTGCT >OriGene 3' read for NM_021705 unedited
GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTATTNTTATAAATTTAT TTTAAAAAATAACAATTGCAATGACATTAATGATCGATCACCTGTACGCCCCCATACTGC TGGGGGAAAAACCTTCGATATAGTTGAATCCCAAAGAAAAATCAAGACATTATTATCAGG CACCCTATAGCCACAAAATGCTCTGTTGGTCCAGTGGCATCGACACTGACATGTTCAATG TACCAGTGCAGTTAAAATATATACACTTAAAATTATCCTGCAAAAAAAAAAAAAAGCACT CAAGTTGCATAACTAGGCATTAAACCAATTATTCATTCATCCATTCACTCTTTCTCCATC TGTAAAGTTATCACTACAGTCACAAATAAAAATGAATAAAAGAACTAGTATCAAAGAAAA AATCCAAATGTCAATACCCTGCCCCCACTTTGTGGGCTACTATGGTGTGCCAAAGTGACC ACAAGAAAAGGGTCATGAGCTATTTGAAATGCAATGACAAAATGACAACTGAAGCATTTC AAACAAAAATTAAAGTCATCTCCCCCCAAATAAAAAAATCAGCACCAAGGCTGGCATTAC TATGTACNAACTTACAGACACAAATAATAGCATCTTTTTACTGTTGGCAGCTTAAGCACT CAAGTTAGGTCTGAGGAATTTTTTTTGACCTCTGCATCTAAACTCCAAATTACCCAAGGA TATAATTCCCCCATGGTAGAAATCTATTTCCTGAAAACTTGGGACTACTGAAAACATTAT ATCCCAACTCTTGGAAAGTAGAGGTTCTTATGAGCTACAAAGGAAAGTCCTTGCATCTGG AGCCAGACGATACCAGAACCGGACTAGAAAATTACCTCCCGTCCCTTGAAAACCGCCCTT TTTTTAAATTAACAATTTTTTGGGGGTTTAAAGGCTAATTTTGCTAAACGGTACCGAGCA AAAAACATTTTTTGGGTTACCCCAGTCCCGAAAAGGTTTCCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021705 |
| Insert Size | 3570 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021705.2, NP_068351.1 |
| RefSeq Size | 2747 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 4800 |
| UniProt ID | P23511 |
| Domains | CBF |
| Protein Families | Transcription Factors |
| Protein Pathways | Antigen processing and presentation |
| Gene Summary | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) is missing alternate exon B, resulting in the short isoform, isoform 2. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Regulation of the p53 transcriptional response by structurally diverse core promoters
,José M. Morachis, Christopher M. Murawsky, and Beverly M. Emerson,
Genes & Dev., Jan 2010; 24: 135 - 147
[NFYA]
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Regulation of the p53 transcriptional response by structurally diverse core promoters
,null,
Genes & Development
,PubMed ID 20040571
[NFYA]
|
|
Nuclear Factor-Y and Epstein Barr Virus in Nasopharyngeal Cancer
,Marie C. Chia, Abby Leung, Tiffany Krushel, Nehad M. Alajez, Kwok W. Lo, Pierre Busson, Henry J. Klamut, Carlo Bastianutto, and Fei-Fei Liu,
Clin. Cancer Res., Feb 2008; 14: 984 - 994.
[NFYA]
|
|
Radiation and Cisplatin activation of EBV in nasopharyngeal cancer: Understanding the oncolytic potential of EBV
,Marie C. Chia, Abby Leung, Tiffaney Krushel, Nehad Alajez, Carlo Bastianutto and Fei-Fei Liu,
Proc Amer Assoc Cancer Res, Volume 47, 2006; Abstract #823
[NFYA]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205981 | NFYA (Myc-DDK-tagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 2 |
CNY 2,400.00 |
|
| RC205981L3 | Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
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| RC205981L4 | Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG205981 | NFYA (tGFP-tagged) - Human nuclear transcription factor Y, alpha (NFYA), transcript variant 2 |
CNY 4,000.00 |
