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MRPS12 (NM_021107) Human Untagged Clone
CAT＃: SC112966
MRPS12 (untagged)-Human mitochondrial ribosomal protein S12 (MRPS12), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112966 sequence for NM_021107 edited (data generated by NextGen Sequencing)
ATGTCCTGGTCTGGCCTTCTCCATGGCCTCAACACGTCCCTAACTTGTGGCCCAGCTCTG GTTCCCCGGCTCTGGGCTACCTGCTCCATGGCTACCCTGAACCAGATGCACCGCCTGGGG CCCCCCAAGCGGCCGCCTCGGAAGCTGGGCCCCACGGAAGGCCGGCCGCAGCTGAAGGGT GTGGTCCTGTGCACGTTTACCCGCAAGCCGAAGAAGCCCAACTCAGCCAATCGCAAGTGC TGTCGAGTGCGGCTCAGCACTGGCCGCGAGGCCGTCTGCTTCATCCCTGGGGAGGGCCAC ACCCTGCAGGAGCACCAGATTGTCCTTGTGGAGGGCGGCCGCACCCAGGACCTGCCAGGC GTCAAGCTCACCGTTGTGCGTGGCAAGTACGACTGTGGCCACGTGCAGAAGAAGTGA Clone variation with respect to NM_021107.1 >OriGene 5' read for NM_021107 unedited
AAAAAGTCGGAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGAGCGT GTCCGCGACCTCACCTTTAGGTCCTGTGAGGTCGGTGGAATCCTGGGGTCCTCCAAATCT ACCAGGCCATCTCCCCAGTTTCCCAGTTCTTCCTGCGTGCGGGCGAGAGTGGTTGGGCCC TCGGGAACCCACTCAGAGCGAGGCTAAATTTACGGAGGGACTTTCTGTTAGCAGCATGAG GGCCTGTGGTTAGACCTATAGAGGTATTTCCTTTGATTTAAGCCAGAAAGTCCTGAGAGC GGATCGGGGAGCATTTGCGGATCGGTCACTTTTTCCTCCTTTCTGAGTCTCTTATCCCCT ACCACAGGGACGGCCCAGGTGGCAGGATGTCCTGGTCTGGCCTTCTCCATGGCCTCAACA CGTCCCTAACTTGTGGCCCAGCTCTGGTTCCCCGGCTCTGGGCTACCTGCTCCATGGCTA CCCTGAACCAGATGCACCGCCTGGGGCCCCCCAAGCGGCCGCCTCGGAAGCTGGGCCCCA CGGAAGGCCGGCCGCAGCTGAAGGGTGTGGTCCTGTGCACGTTTACCCGCAAGCCGAAGA AGCCCAACTCAGCCAATCGCAAGTGCTGTCGAGTGCGGCTCAGCACTGGCCGCGAGGCCG TCTGCTTCATCCCTGGGGAGGGCCACACCCTGCAGGAGCACCAGATTGTCCTTGTGNGAG GGCGGCCGCACCCAGGACCTGCCAGGCGTCAAGCTCACCGTTGTGCGTGGCAAGTACGAC TGTGGCCACGTGCAAAATAATGACGGCTGGGGCCCCATGGGGCCTGGGCGCCCCTGCAGA ACATGAACCTTTCGCTCCTGGCTGCCACAG >OriGene 3' read for NM_021107 unedited
CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACATCACAAGCGCCAGGGTC TGTTTATTAAAACCCCTTCCCAGCAGAGGGCAGTACAGTGTCTTGTCCCAGCAGAAAAGG TCCTTCAGGCCACCCCTCTAAACACAGGAGCCAAGGGTGTTGACAGAGGGGCACCCTTTG CACCCCCAGGACTCCTATGGCTTAATAGTGGTCCTGATGGAAGAGGCGGAGCCAGGACTT GAATCCATGAGTGGCTGCCTCTAGAGGCGCAAAGGCCAGCATCGGAGGACCCTGTGGCAG CCAGGAGCGGAAGGTTCATGTTCTGCAGGGGCGCCCAGCCCACTGTGCCCCCAGCCGTCA CTTCTTCTGCACGTGGCCACAGTCGTACTTGCCACGCACAACGGTGAGCTTGACGCCTGG CAGGTCCTGGGTGCGGCCGCCCTCCACAAGGACAATCTGGTGCTCCTGCAGGGTGTGGCC CTCCCCAGGGATGAAGCAGACGGCCTCGCGGCCAGTGCTGAGCCGCACTCGACAGCACTT GCGATTGGCTGAGTTGGGCTTCTTCGGCTTGCGGGTAAACGTGCACAGGACCACACCCTT CAGCTGCGGCCGGCCTTCCGTGGGGCCCAGCTTCCGAGGCGGCCGCTTGGGGGGCCCCAG GCGGTGCATCTGGTTCAGGGTAGCCCTGGAGCATGTAGCCCCAGAGCCGGGACCAGAGCT GGGCCACAGTTANGGACGTGTTGAGCCATGGAGATGCCAATACCAGACATCCCTGCACCT GGGCCGTNCCTGTGGTAGGGGATANNAGACTCANAAAGGAGGAAAAGTGACCGATCCGCA AAGCCTCCCGATCCGCTCTTCAGACTTTCTGTCTAAAATCAAGGAATTACTCTTATAGTC TACCCACAGCCCTAAGCTGCTACAGAAAGTCCTCCGTAAATTAACCCCCTCTGAAGGGTC CCCAGGCCCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021107 |
| Insert Size | 1150 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021107.1, NP_066930.1 |
| RefSeq Size | 1094 bp |
| RefSeq ORF | 417 bp |
| Locus ID | 6183 |
| UniProt ID | O15235 |
| Domains | Ribosomal_S12 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the longest transcript, when the polyA tail is excluded. Variants 1, 2, and 3 encode the same isoform. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222489 | MRPS12 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S12 (MRPS12), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 1,200.00 |
|
| RC222489L3 | Lenti-ORF clone of MRPS12 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S12 (MRPS12), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RC222489L4 | Lenti-ORF clone of MRPS12 (mGFP-tagged)-Human mitochondrial ribosomal protein S12 (MRPS12), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RG222489 | MRPS12 (tGFP-tagged) - Human mitochondrial ribosomal protein S12 (MRPS12), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,370.00 |
