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CDK4 (NM_000075) Human Untagged Clone
CAT＃: SC112998
CDK4 (untagged)-Human cyclin-dependent kinase 4 (CDK4)
CNY 3,600.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMM3; PSK-J3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112998 sequence for NM_000075 edited (data generated by NextGen Sequencing)
ATGGCTACCTCTCGATATGAGCCAGTGGCTGAAATTGGTGTCGGTGCCTATGGGACAGTG TACAAGGCCCGTGATCCCCACAGTGGCCACTTTGTGGCCCTCAAGAGTGTGAGAGTCCCC AATGGAGGAGGAGGTGGAGGAGGCCTTCCCATCAGCACAGTTCGTGAGGTGGCTTTACTG AGGCGACTGGAGGCTTTTGAGCATCCCAATGTTGTCCGGCTGATGGACGTCTGTGCCACA TCCCGAACTGACCGGGAGATCAAGGTAACCCTGGTGTTTGAGCATGTAGACCAGGACCTA AGGACATATCTGGACAAGGCACCCCCACCAGGCTTGCCAGCCGAAACGATCAAGGATCTG ATGCGCCAGTTTCTAAGAGGCCTAGATTTCCTTCATGCCAATTGCATCGTTCACCGAGAT CTGAAGCCAGAGAACATTCTGGTGACAAGTGGTGGAACAGTCAAGCTGGCTGACTTTGGC CTGGCCAGAATCTACAGCTACCAGATGGCACTTACACCCGTGGTTGTTACACTCTGGTAC CGAGCTCCCGAAGTTCTTCTGCAGTCCACATATGCAACACCTGTGGACATGTGGAGTGTT GGCTGTATCTTTGCAGAGATGTTTCGTCGAAAGCCTCTCTTCTGTGGAAACTCTGAAGCC GACCAGTTGGGCAAAATCTTTGACCTGATTGGGCTGCCTCCAGAGGATGACTGGCCTCGA GATGTATCCCTGCCCCGTGGAGCCTTTCCCCCCAGAGGGCCCCGCCCAGTGCAGTCGGTG GTACCTGAGATGGAGGAGTCGGGAGCACAGCTGCTGCTGGAAATGCTGACTTTTAACCCA CACAAGCGAATCTCTGCCTTTCGAGCTCTGCAGCACTCTTATCTACATAAGGATGAAGGT AATCCGGAGTGA Clone variation with respect to NM_000075.3 >OriGene 5' read for NM_000075 unedited
ACCATTAGTATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGTCTATGGTCG GGCCCTCTGCGTCCAGCTGCTCCGGACCGAGCTCGGGTGTATGGGGCCGTAGGAACCGGC TCCGGGGCCCCGATAACGGGCCGCCCCCACAGCACCCCGGGCTGGCGTGAGGGTCTCCCT TGATCTGAGAATGGCTACCTCTCGATATGAGCCAGTGGCTGAAATTGGTGTCGGTGCCTA TGGGACAGTGTACAAGGCCCGTGATCCCCACAGTGGCCACTTTGTGGCCCTCAAGAGTGT GAGAGTCCCCAATGGAGGAGGAGGTGGAGGAGGCCTTCCCATCAGCACAGTTCGTGAGGT GGCTTTACTGAGGCGACTGGAGGCTTTTGAGCATCCCAATGTTGTCCGGCTGATGGACGT CTGTGCCACATCCCGAACTGACCGGGAGATCAAGGTAACCCTGGTGTTTGAGCATGTAGA CCAGGACCTAAGGACATATCTGGACAAGGCACCCCCACCAGGCTTGCCAGCCGAAACGAT CAAGGATCTGATGCGCCAGTTTCTAAGAGGCCTAGATTTCCTTCATGCCAATTGCATCGT TCACCGAGATCTGAAGCCAGAGAACATTCTGGTGACAAGTGGTGGAACAGTCAAGCTGGC TGACTTTGGCCTGGCCAGAATCTACAGCTACCAGATGGCACTCACACCCGTGGTTGTTAC ACTCTGGTACCGAGCTCCCGAAGTTCTTCTGCAGTCCACATATGCCACACCTGTGGACAT GTGTAGTGTTGGCTGTATCTTTGCAGAGATGTTCCGTCGGAAGCCTCTCTCCTGTGGAAA CTCTGAAACCGACCAGGTGGGGCAAAACTTTGCCCTGANTGGCTGCCTCCACAGTAGACT GTCCTCGAGAGTATCCCTGCCCCGTGAAGCCCTTTCCCCCAAAGG >OriGene 3' read for NM_000075 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAATCTATATTCTTT TTTTTTTTTTTGACACAGAGTCTTGCTCTGTTGCCCAAGCTGGAGTGCAATGGCATGATC TCGGCTCACCGCAACCTCTGCCTCCCGGGTTCAAGCGATCCTCCTGCCTCAGTCTCCCAA GTAGCTGGGATTACAGGTGTGCACCACCACGCCTGACTAATTTTGTATTTTTAGTAAAGA CAGGGTTTCACCATGTTAGTCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCG CGTTGGCCTCCCAACGTGCTGGGATTATAGGCGTGAGCCACCACGCCCCGCCTAAAATCC ATATTCAAAGAAGCAATTTCAGTTCCTTTCTAAGCTTTGTCAGTCAAGGGGCTCCACTGA CTTCCTAGGCCCTGTAATTTAACCAGTCTTTAAGGTTTTGCAGGAAAGTCCCTTCTTCCA AGTGGTTTTTCCAAATCGCACAATGGCAAAGCCAAACCGAGGAAGAAACATTAAAAAAAA AAAAAAGACCATTATTTCTTTGTTTCGCTTTTCCTGTATAAAAAAGGACCCCCAATATAA AGGTAGGGAAAGGGACAAGAGGGAACATACCCCTTACTGTATAGAAATGGGAAGGAGAAG GAAAACCCTCCAAAGGAGAGGTGGGAGGGGACTGTCATTTAAGGCGCAAAAGTATTCTCT GTACACAGATGGAGGAAGACCTTCCATATCCTCAGAGATAAAGGCCAAAATTGCCCTTTC ACTCGTCACAAGGAAAAGGGAGCTTTTCTTCTTTCCTGGCAGCCACTCCTTTGCTCCTTC CGGATACCTTCTCCTTATGTAAATAAGAGGCTGCAAACCCTGAAAAGCATATATTGCTTG TGGGGCTTAAAGTCAAAATTTCCCCACAGCTGGGCTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000075 |
| Insert Size | 912 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000075.2, NP_000066.1 |
| RefSeq Size | 1474 bp |
| RefSeq ORF | 912 bp |
| Locus ID | 1019 |
| UniProt ID | P11802 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer, T cell receptor signaling pathway, Tight junction |
| Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the full length protein. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MicroRNA-195 inhibits epithelial-mesenchymal transition via downregulating CDK4 in bladder cancer
,Chen, S;Wang, W;Lin, G;Zhong, S;,
Int J Clin Exp Pathol
[CDK4]
|
|
MicroRNA-195 inhibits epithelial-mesenchymal transition via downregulating CDK4 in bladder cancer
,null,
International Journal of Clinical and Experimental Pathology
,PubMed ID 31949777
[CDK4]
|
|
Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells
,Kim, YC;Chen, C;Bolton, EC;,
PLoS ONE
,PubMed ID 26372468
[CDK4]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201156 | CDK4 (Myc-DDK-tagged)-Human cyclin-dependent kinase 4 (CDK4) |
CNY 3,600.00 |
|
| RC201156L1 | Lenti ORF clone of Human cyclin-dependent kinase 4 (CDK4), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC201156L2 | Lenti ORF clone of Human cyclin-dependent kinase 4 (CDK4), mGFP tagged |
CNY 5,890.00 |
|
| RC201156L3 | Lenti ORF clone of Human cyclin-dependent kinase 4 (CDK4), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC201156L4 | Lenti ORF clone of Human cyclin-dependent kinase 4 (CDK4), mGFP tagged |
CNY 6,000.00 |
|
| RG201156 | CDK4 (tGFP-tagged) - Human cyclin-dependent kinase 4 (CDK4) |
CNY 4,370.00 |
|
| SC323451 | CDK4 (untagged)-Kinase deficient mutant (K35M) of Human cyclin-dependent kinase 4 (CDK4) |
CNY 3,600.00 |
|
| SC323460 | CDK4 (untagged)-Kinase deficient mutant (K35M) of Human cyclin-dependent kinase 4 (CDK4) |
CNY 3,600.00 |
|
| SC323699 | CDK4 (untagged)-Kinase deficient mutant (K35M) of Human cyclin-dependent kinase 4 (CDK4) |
CNY 3,600.00 |
