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CKMT1B (NM_020990) Human Untagged Clone
CAT＃: SC113062
CKMT1B (untagged)-Human creatine kinase, mitochondrial 1B (CKMT1B), nuclear gene encoding mitochondrial protein
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CKMT; CKMT1; UMTCK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_020990, the custom clone sequence may differ by one or more nucleotides
ATGGCTGGTCCCTTCTCCCGTCTGCTGTCCGCCCGCCCGGGACTCAGGCTCCTGGCTTTGGCCGGAGCGG GGTCTCTAGCCGCTGGGTTTCTGCTCCGACCGGAACCTGTACGAGCTGCCAGTGAACGACGGAGGCTGTA TCCCCCGAGCGCTGAGTACCCAGACCTCCGAAAGCACAACAACTGCATGGCCAGTCACCTGACCCCAGCA GTCTATGCACGGCTCTGCGACAAGACCACACCCACTGGTTGGACGCTAGATCAGTGTATCCAGACTGGCG TGGACAACCCTGGCCACCCCTTCATCAAGACTGTGGGCATGGTGGCTGGAGATGAGGAGACCTATGAGGT ATTTGCTGACCTGTTTGACCCTGTGATCCAAGAGCGACACAATGGATATGACCCCCGGACAATGAAGCAC ACCACGGATCTAGATGCCAGTAAAATCCGTTCTGGCTACTTTGATGAGAGGTATGTATTGTCCTCTAGAG TCAGAACTGGCCGAAGCATCCGAGGACTCAGTCTGCCTCCAGCTTGCACTCGAGCAGAGCGACGAGAGGT GGAACGTGTTGTGGTGGATGCACTGAGTGGCCTGAAGGGTGACCTGGCTGGACGTTACTATAGGCTCAGT GAGATGACAGAGGCTGAACAGCAGCAGCTTATTGATGACCACTTTCTGTTTGATAAGCCTGTGTCCCCGT TGCTGACTGCAGCAGGAATGGCTCGAGACTGGCCAGATGCTCGTGGAATTTGGCACAACAATGAGAAGAG CTTCCTGATCTGGGTGAATGAGGAGGATCATACACGGGTGATCTCCATGGAGAAGGGTGGTAACATGAAG AGAGTGTTTGAAAGATTCTGCCGAGGCCTCAAAGAGGTGGAGAGACTTATCCAAGAACGTGGCTGGGAGT TCATGTGGAATGAGCGTTTGGGATACATCTTGACCTGTCCATCTAACCTGGGCACTGGACTTCGGGCAGG AGTGCACATCAAACTGCCCCTGCTAAGCAAAGATAGCCGCTTCCCAAAGATCCTGGAGAACCTAAGACTC CAAAAACGTGGTACTGGAGGAGTGGACACTGCTGCTACAGGCGGTGTCTTTGATATTTCTAATTTGGACC GACTAGGCAAATCAGAGGTGGAGCTGGTGCAACTGGTCATCGATGGAGTAAACTATTTGATTGATTGTGA ACGGCGTCTGGAGAGAGGCCAGGATATCCGCATCCCCACACCTGTCATCCACACCAAGCATTAA >OriGene 5' read for NM_020990 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCCATTCCGGCTCC AGCCTCCAATCCGACCCCCATTTCGGCTGCAGCCTCGGACCTAGCTCCGGCCCTCGGTCT ATCCGGTTGCATCCTCCCTCCCTGTTCCGGATCTTATCTTGCGCCAGCGCCTACTCCAGG ATCCCGTAGCCAGACCTCAAGCCATGGCTGGTCCCTTCTCCCGTCTGCTGTCCGCCCGCC CGGGACTCAGGCTCCTGGCTTTGGCCGGAGCGGGGTCTCTAGCCGCTGGGTTTCTGCTCC GACCGGAACCTGTACGAGCTGCCAGTGAACGACGGAGGCTGTATCCCCCGAGCGCTGAGT ACCCAGACCTCCGAAAGCACAACAACTGCATGGCCAGTCACCTGACCCCAGCAGTCTATG CACGGCTCTGCGACAAGACCACACCCACTGGTTGGACGCTAGATCAGTGTATCCAGACTG GCGTGGACAACCCTGGCCACCCCTTCATCAAGACTGTGGGCATGGTGGCTGGAGATGAGG AGACCTATGAGGTATTTGCTGACCTGTTTGACCCTGTGATCCAAGAGCGACACAATGGAT ATGACCCCCGGACAATGAAGCACACCACGGATCTAGATGCCAGTAAAATCCGTTCTGGCT ACTTTGATGAGAGGTATGTATTGTCCTCTAGAGTCAGAACTGGCCGAAGCATCCGAGGAC TCAGTCTGCCTCCAGCTTGCACTCGAGCAGAGCGACGAGAGGTGGAACGTGTTGTGGNTG ATGCACTGAGTGGCCTGAAGGGTGACCTGGCTGGNACGTACTATAGGCTCAGTGAGATGA CAGAGGGCTGACAGCAGCAGCTTATTGATGACCACTNTCTNTNGATAAGCCTGTGTNCCC CGTGCTG >OriGene 3' read for NM_020990 unedited
CTATGGACCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAACCAAAACAGGGGGTTT TTACTAGGATGGGGGCAGGGAATGCGGGGCCAGGCCCAAACCAAGAAAAAGGGCCCTTTT TTAAAAGGACCAAACCCCCGGGAAATTTTGAGCCATCACCTGGCAAGGGGGATTAAATGC TTGGGGGGGAAAACAGGGGGGGGAATGCGAATTTCCGGGCCTTTTTCCAAACCCCGTTAA AAATAAATAAAATAGTTAACTCCTTGGAGGACCAGTTGCCCCACCCCCCCCTTTGATTTG CCTAGCCGGCCCAAATTAAAAATATAAAAAACCCCCCCTGTACAAAAAGGGCCCACTCCT CCAAACCCACTTTTTGGGAGTTTAAGGTTCCCCAGAATTTTTGGAAAACGGTTTTTTTTT TTTACCAGGGGCATTTTGATGGCCCCTCCTGCCCAAATCCCAGGGCCCAGGTTAAAGGGA CAGGGAAAAATGTATCCCAAACGCTATTTCCAAATGAACTCCCCACCCCGTTCTTGGAAA AGTCTTTCCCACCTCTTGGGGCCCCCGCAGAATTTTTAAAACTCTCTTTTAATGGTACCC CCCCTTTTCATGGGAAAACACCGGGTATTGACCCTCCTAATTCCCCCAAAACAGGAAGTT TTTTTAATTGTGTGGCCAAATTCCACGACCATGTGGCCAGTCTTAGACCCATTCTGGTGG AATAAGCACCGGGAACCCAGTTTTAACAAACAAAAGGGGCCATAAAAGACTGGTGGTGTA AACCCTGTTTTTCCCTGGGCCTTAAGAAACGGCCAGCCGGGCCCCTTTTAGGCCATTAGG GGATCACCCCACACAGTCCCCCTTTTTNCCTTTGTTAAAGAGGAAAGGGGGCAAACTGAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020990 |
| Insert Size | 1460 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020990.3, NP_066270.1 |
| RefSeq Size | 1779 bp |
| RefSeq ORF | 1254 bp |
| Locus ID | 1159 |
| UniProt ID | P12532 |
| Domains | ATP-gua_Ptrans |
| Protein Families | Druggable Genome |
| Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
| Gene Summary | Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212558 | CKMT1B (Myc-DDK-tagged)-Human creatine kinase, mitochondrial 1B (CKMT1B), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
| RC212558L3 | Lenti ORF clone of Human creatine kinase, mitochondrial 1B (CKMT1B), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212558L4 | Lenti ORF clone of Human creatine kinase, mitochondrial 1B (CKMT1B), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG212558 | CKMT1B (tGFP-tagged) - Human creatine kinase, mitochondrial 1B (CKMT1B), nuclear gene encoding mitochondrial protein |
CNY 5,256.00 |
