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VPS35 (NM_018206) Human Untagged Clone
CAT＃: SC113645
VPS35 (untagged)-Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)
CNY 8,752.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MEM3; PARK17 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113645 sequence for NM_018206 edited (data generated by NextGen Sequencing)
ATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAAGCTCTTGGATGAAGCCATA CAGGCTGTGAAGGTCCAGTCATTCCAAATGAAGAGATGCCTGGACAAAAACAAGCTTATG GATGCTCTAAAACATGCTTCTAATATGCTTGGTGAACTCCGGACTTCTATGTTATCACCA AAGAGTTACTATGAACTTTATATGGCCATTTCTGATGAACTGCACTACTTGGAGGTCTAC CTGACAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATCTCTACGAACTTGTACAGTAT GCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGTTGGAGTTGTATATGTCAAG TCATTTCCTCAGTCCAGGAAGGATATTTTGAAAGATTTGGTAGAAATGTGCCGTGGTGTG CAACATCCCTTGAGGGGTCTGTTTCTTCGAAATTACCTTCTTCAGTGTACCAGAAATATC TTACCTGATGAAGGAGAGCCAACAGATGAAGAAACAACTGGTGACATCAGTGATTCCATG GATTTTGTACTGCTCAACTTTGCAGAAATGAACAAGCTCTGGGTGCGAATGCAGCATCAG GGACATAGCCGAGATAGAGAAAAAAGAGAACGAGAAAGACAAGAACTGAGAATTTTAGTG GGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAGGTGTAAATGTGGAACGTTACAAACAG ATTGTTTTGACTGGCATATTGGAGCAAGTTGTAAACTGTAGGGATGCTTTGGCTCAAGAA TATCTCATGGAGTGTATTATTCAGGTTTTCCCTGATGAATTTCACCTCCAGACTTTGAAT CCTTTTCTTCGGGCCTGTGCTGAGTTACACCAGAATGTAAATGTGAAGAACATAATCATT GCTTTAATTGATAGATTAGCTTTATTTGCTCACCGTGAAGATGGACCTGGAATCCCAGCG GATATTAAACTTTTTGATATATTTTCACAGCAGGTGGCTACAGTGATACAGTCTAGACAA GACATGCCTTCAGAGGATGTTGTATCTTTACAAGTCTCTCTGATTAATCTTGCCATGAAA TGTTACCCTGATCGTGTGGACTATGTTGATAAAGTTCTAGAAACAACAGTGGAGATATTC AATAAGCTCAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGAACTCACCAGA CTTTTGAAAATACCAGTTGACACTTACAACAATATTTTAACAGTCTTGAAATTAAAACAT TTTCACCCACTCTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGTTGTTATGTG CTTAGTAATGTTCTGGATTATAACACAGAAATTGTCTCTCAAGACCAGGTGGATTCCATA ATGAATTTGGTATCCACGTTGATTCAAGATCAGCCAGATCAACCTGTAGAAGACCCTGAT CCAGAAGATTTTGCTGATGAGCAGAGCCTTGTGGGCCGCTTCATTCATCTGCTGCGCTCT GAGGACCCTGACCAGCAGTACTTGATTTTGAACACAGCACGAAAACATTTTGGAGCTGGT GGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTACCAGCTGGCT TTTCGATATAAAGAGAATTCTAAAGTGGATGACAAATGGGAAAAGAAATGCCAGAAGATT TTTTCATTTGCCCACCAGACTATCAGTGCTTTGATCAAAGCAGAGCTGGCAGAATTGCCC TTAAGACTTTTTCTTCAAGGAGCACTAGCTGCTGGGGAAATTGGTTTTGAAAATCATGAG ACAGTCGCATATGAATTCATGTCCCAGGCATTTTCTCTGTATGAAGATGAAATCAGCGAT TCCAAAGCACAGCTAGCTGCCATCACCTTGATCATTGGCACTTTTGAAAGGATGAAGTGC TTCAGTGAAGAGAATCATGAACCTCTGAGGACTCAGTGTGCCCTTGCTGCATCCAAACTT CTAAAGAAACCTGATCAGGGCCGAGCTGTGAGCACCTGTGCACATCTCTTCTGGTCTGGC AGAAACACGGACAAAAATGGGGAGGAGCTTCACGGAGGCAAGAGGGTAATGGAGTGCCTA AAAAAAGCTCTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTGCAGCTTTTT ATAGAAATTCTGAACAGATATATCTATTTTTATGAAAAGGAAAATGATGCGGTAACAATT CAGGTTTTAAACCAGCTTATCCAAAAGATTCGAGAAGACCTCCCGAATCTTGAATCCAGT GAAGAAACAGAGCAGATTAACAAACATTTTCATAACACACTGGAGCATTTGCGCTTGCGG CGGGAATCACCAGAATCCGAGGGGCCAATTTATGAAGGTCTCATCCTTTAA Clone variation with respect to NM_018206.4 >OriGene 5' read for NM_018206 unedited
TAGCATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGCTTGCTG CAGGCTCTGGGGAGTCGCCATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAA GCTCTTGGATGAAGCCATACAGGCTGTGAAGGTCCAGTCATTCCAAATGAAGAGATGCCC GGACAAAAACAAGCTTATGGATGCTCTAAAACATGCTTCTAATATGCTTGGTGAACTCCG GACTTCTATGTTATCACCAAAGAGTTACTATGAACTTTATATGGCCATTTCTGATGAACT GCACTACTTGGAGGTCTACCTGACAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATCT CTACGAACTTGTACAGTATGCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGT TGGAGTTGTATATGTCAAGTCATTTCCTCAGTCCAGGAAGGATATTTTGAAAGATTTGGT AGAAATGTGCCGTGGTGTGCAACATCCCTTGAGGGGTCTGTTTCTTCGAAATTACCTTCT TCAGTGTACCAGAAATATCTTACCTGATGAAGGAGAGCCAACAGATGAAGAAACAACTGG TGACATCAGTGATTCCATGGATTTTGTACTGCTCAACTTTGCAGAAATGAACAAGCTCTG GGTGCGAATGCAGCATCAGGGACATAGCCGAGATAGAGAAAAAAGAGAACGAGAAAGACA AGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAGGTGTAAA TGTGGAACGTTACCAACAGATTGTTTTGACTGGCATATTGGAGCCAAGTGTAAACTGTAG GGATGCTTTGGCTCAAGATATCTCATGGAGTGTATTATTCAAGCTCTTCCTGATGATTTC ACCTCCAGACTTGAATNNCTTTCTTCGGGCCTGTGCTGAATACACCAAATGTAN >OriGene 3' read for NM_018206 unedited
GACGCGCCGACGCNCCCCCACTTCCTACCCNNNGGGNNGTACTGTGNNANGAAAACACGC NTTCTTAGGANCGNGNTATTATCCTTTTTTTTTTCACGGATCAAGGNATTTTAATTGCAG ATCAGTCATGCTACTTGGGGTGATCAGCAAGCACCTTGAACACTCTACCAAGCTGAATAA TTTTATTAAGGTCCTGAAGGTGAGTGTCCGGAGGTGCTGGNGTAAAACACATCACAGGTA AGAAATGGGAAAACCTACCTCAGCATTTCTGAAAGGCACAATCTATGGAAGGGAAACCTA GCGTAATAAAACCCTCACTGGATGTACATGGAAAGGAGTATGGTGAGCTATTTCCTTTTT AAAGGATGAGACCTTCATAAATTGGCCCCCTCGATTCTGGTGATTCCCGCCGCAAGCGCA AATGCTCCAGTGTGTTATGAAAATGTTTGTTAATCTGCTCTGTTTCTTCACTGGATTCAA GATTCGGGAGGTCTTCTCGAATCTTTTGGATAAGCTGGTTTAAAACCTGAATTGTTACCG CATCATTTTCCTTTTCATAAAAATAGATATATCTGTTCAGAATTTCTATAAAAAGCTGCA CTTGCAGAGAGGGGTCCATGCACTGATTTGCTATTTTTAGAGCTTTTTTTAGGCACTCCA TTACCCTCTTGCCTCCGAGAAGCTCCCTCCCCATTTCTGTCCGTGGTTCTGCCCGACCAA AAGAGATGTGCCCAGGGGCTCACAGCTCGGCCCTGAACAGGGTTCTTTTAAAGTTTGCAT GCCCCAGGGCCCCCTGAGTCCCCAAAGTTCATGAATTTTTTACCTGAACCATTTATTCCT TCCAAAAGGCCCATGCACCAAAGGGGAGGCACCCTCCCTGGGCCTTGGAAACCCCGGATT CAACTCCATACAAAAAAAAAGCCTGGGCACATGATTCCTAGCCACCGGCCCCCGGATTTT AAAAACCAATTCTCCCAACAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018206 |
| Insert Size | 2680 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018206.3, NP_060676.2 |
| RefSeq Size | 3246 bp |
| RefSeq ORF | 2391 bp |
| Locus ID | 55737 |
| UniProt ID | Q96QK1 |
| Domains | Vps35 |
| Gene Summary | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210597 | VPS35 (Myc-DDK-tagged)-Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35) |
CNY 8,744.00 |
|
| RC210597L1 | Lenti ORF clone of Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), Myc-DDK-tagged |
CNY 11,144.00 |
|
| RC210597L2 | Lenti ORF clone of Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), mGFP tagged |
CNY 11,144.00 |
|
| RC210597L3 | Lenti ORF clone of Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), Myc-DDK-tagged |
CNY 11,144.00 |
|
| RC210597L4 | Lenti ORF clone of Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), mGFP tagged |
CNY 11,144.00 |
|
| RG210597 | VPS35 (tGFP-tagged) - Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35) |
CNY 10,344.00 |
