IARS2 (NM_018060) Human Untagged Clone
CAT#: SC113746
IARS2 (untagged)-Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein
CNY 10,576.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CAGSSS; ILERS |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_018060, the custom clone sequence may differ by one or more nucleotides
ATGCGTTGGGGGCTGCGCCCTCGCGGGCCGGGCGCGGCCGCCCTGGCCACTGCCCGAAGTTTGTGGGGGA CGCCCCGCCTTCCCTGCAGCCCGGGATGGCAAGGGGCGACGAAGAGGCTTCTGGTGCGGTCGGTCTCCGG GGCCAGTAACCACCAGCCGAACTCGAATAGTGGCAGATACCGGGACACGGTGCTGCTGCCGCAGACGAGC TTCCCCATGAAGCTGCTGGGCCGCCAGCAGCCGGACACGGAGCTGGAGATCCAGCAGAAATGTGGATTTT CAGAACTTTATTCATGGCAAAGAGAAAGAAAAGTAAAGACAGAATTTTGCCTTCATGATGGACCTCCTTA TGCAAACGGTGACCCTCATGTTGGACATGCTTTAAATAAGATTTTGAAAGACATAGCCAATCGATTCCAT ATGATGAATGGCTCCAAAATACATTTTGTGCCCGGCTGGGATTGTCATGGGTTGCCCATTGAAATAAAAG TATTATCAGAACTTGGTAGAGAAGCTCAGAATCTTTCAGCTATGGAAATTAGAAAGAAAGCTAGATCATT TGCTAAAGCAGCCATTGAGAAACAGAAATCAGCATTTATTCGTTGGGGAATAATGGCAGATTGGAATAAT TGCTACTATACATTTGATGGGAAGTATGAAGCCAAACAGTTGAGAACTTTTTACCAAATGTATGATAAGG GCTTGGTTTATCGATCTTACAAACCTGTGTTTTGGTCTCCGTCATCTAGGACTGCATTGGCTGAAGCAGA ACTTGAATATAATCCTGAGCATGTCAGTCGTTCAATATATGTAAAATTTCCTCTCTTAAAGCCTTCTCCA AAATTGGCATCTCTTATAGATGGTTCATCTCCTGTTAGTATTTTGGTCTGGACCACACAACCTTGGACGA TTCCAGCCAATGAAGCTGTTTGCTATATGCCTGAATCAAAGTATGCTGTTGTGAAATGTTCTAAGTCTGG AGACCTCTACGTACTGGCGGCAGATAAAGTAGCATCTGTTGCTTCTACTTTGGAAACAACATTTGAGACT ATTTCAACACTTTCAGGTGTAGATTTGGAAAATGGTACTTGCAGTCATCCATTAATTCCTGATAAAGCCT CTCCTCTTTTACCTGCAAATCATGTGACCATGGCAAAAGGAACGGGATTGGTTCACACAGCCCCAGCTCA TGGTATGGAAGACTACGGTGTAGCGTCTCAGCACAACCTGCCCATGGATTGTCTAGTGGACGAAGATGGA GTTTTCACAGATGTTGCAGGTCCTGAACTTCAAAACAAGGCTGTCCTTGAAGAGGGAACTGATGTGGTTA TAAAGATGCTTCAGACTGCAAAGAATTTGTTGAAAGAGGAGAAATTGGTGCATAGCTATCCGTATGACTG GAGGACCAAGAAACCTGTGGTTATTCGTGCCAGCAAGCAGTGGTTTATAAACATCACGGATATTAAGACT GCAGCCAAGGAATTGTTAAAAAAGGTGAAATTTATTCCTGGATCAGCACTGAATGGCATGGTTGAAATGA TGGACAGGCGGCCATATTGGTGTATATCAAGGCAAAGAGTTTGGGGTGTTCCAATTCCTGTGTTTCATCA TAAGACCAAGGATGAATACTTGATCAACAGCCAAACCACTGAGCATATTGTTAAACTAGTGGAACAACAC GGCAGTGATATCTGGTGGACTCTTCCCCCTGAACAACTTCTTCCAAAAGAAGTCTTATCTGAGGTTGGTG GCCCTGATGCCTTGGAATATGTGCCAGGTCAGGATATTTTGGACATCTGGTTTGATAGCGGAACTTCATG GTCTTATGTTCTTCCAGGTCCTGACCAAAGAGCAGATTTGTACTTGGAAGGAAAAGACCAGCTCGGGGGT TGGTTTCAGTCATCCTTATTAACAAGTGTGGCAGCAAGGAAGAGAGCACCTTATAAGACAGTGATTGTTC ATGGATTTACCCTTGGAGAAAAGGGAGAAAAGATGTCCAAGTCTCTTGGGAATGTCATTCATCCTGATGT TGTCGTTAATGGAGGACAAGATCAAAGCAAAGAGCCTCCGTATGGTGCTGATGTCCTTCGCTGGTGGGTA GCTGATTCCAATGTCTTCACCGAAGTTGCAATTGGCCCATCCGTGCTCAATGCTGCCAGAGATGATATTA GCAAGCTTAGGAATACACTTCGCTTTCTTTTGGGAAATGTGGCTGATTTCAACCCAGAAACAGATTCCAT CCCTGTAAACGATATGTATGTCATAGACCAGTACATGCTACACTTACTGCAGGATTTGGCAAACAAGATT ACCGAATTATACAAACAATATGATTTTGGAAAAGTTGTTCGGCTGTTACGGACGTTTTATACCAGAGAGC TCTCTAACTTTTATTTCAGTATAATCAAAGATAGGCTCTATTGTGAAAAGGAAAATGACCCCAAACGACG CTCTTGTCAGACTGCATTAGTTGAAATTTTGGATGTAATAGTTCGTTCTTTTGCTCCCATTCTTCCTCAC CTGGCTGAAGAGGTGTTCCAGCACATACCTTATATTAAAGAGCCCAAGAGTGTTTTCCGTACTGGGTGGA TTAGTACTAGTTCTATCTGGAAAAAGCCCGGGTTGGAAGAAGCTGTGGAGAGTGCGTGTGCAATGCGAGA CTCATTTCTTGGAAGCATCCCTGGCAAAAATGCAGCTGAGTACAAGGTTATCACTGTGATAGAACCTGGA CTGCTTTTTGAGATAATAGAGATGCTGCAGTCTGAAGAGACTTCCAGCACCTCTCAGTTGAATGAATTAA TGATGGCTTCTGAGTCAACTTTACTGGCTCAGGAACCACGAGAGATGACTGCAGATGTAATCGAGCTTAA AGGGAAATTCCTCATCAACTTAGAAGGTGGTGATATTCGTGAAGAGTCTTCCTATAAAGTAATTGTCATG CCGACTACGAAAGAAAAATGCCCCCGTTGTTGGAAGTATACAGCGGAGTCTTCAGATACACTGTGTCCTC GATGTGCAGAAGTTGTCAGTGGAAAATAG >OriGene 5' read for NM_018060 unedited
AGGGCGGCCACGACATTCGCACGAGGTCTTACTCGGCTCCCCTTGGTTTCCTGGGGTCCT GCCCCTTCAAGCTGGGGCGGGAGCGGAGGACCCCGCTCTCAGGGGTTGCCGGACCATGCG TTGGGGGCTGCGCCCTCGCGGGCCGGGCGCGGCCGCCCTGGCCACTGCCCGAAGTTTGTG GGGGATCGCTCCTCCTTCCCTGCAGCCCGGGATGGCAAGGGGCGACGAAGAGGCTTCTGG TGCGGTCGGTCTCCGGGGCCAGTAACCACCAGCCGAACTCGAATAGTGGCAGATACCGGG ACACGGTGCTGCTGCCGCAGACGAGCTTCCCCATGAAGCTGCTGGGCCGCCAGCAGCCGG ACACGGAGCTGGAGATCCAGCAGAAATGTGGATTTTCAGAACTTTATTCATGGCAAAGAG AAAGAAAAGTAAAGACAGAATTTTGCCTTCATGATGGACCTCCTTATGCAAACGGTGACC CTCATGTTGGACATGCTTTAAATAAGATTTTGAAAGACATAGCCAATCGATTCCATATGA TGAATGGCTCCAAAATACATTTTGTGCCCGGCTGGGATTGTCATGGGTTGCCCATTGAAA TAAAAGTATTATCAGAACTTGGTAGAGAAGCTCAGAATCTTTCAGCTATGGAAATTAGAA AGAAAGCTAGATCATTTGCTAAAGCAGCCATTGAGAAACAGAAATCAGCATTTATTCGTT GGGGAATAATGGCAGATTGGAATAATTGCTACTATACATTTGATGGGAAGTATGAAGCCC AAACAGTTGAGACTTTTTACCAAATGTATGATAAGGGCTTGGTTTATCGATCTTACAAAC CTGTGTTTGTCTCCGTCATCTAGGACTGCATTGGCTGAAGCAGAACTG >OriGene 3' read for NM_018060 unedited
CGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATTTTTCCAGAATGCCTTTAT TTTCAGTATCATAGAATTTAAATACAGAGTCAAAAGATGATTTATAAAATATAAAACATT TTCTGCTTGGCCGTATTTGAAGACAAGCTGAATACATATCTATGTTCTGAATAAGTCCAC TATGGATATATATAGGAAGAGATATACATATATCCATCCACAGATACACACACACATATA TATTTCTGCATGTATATATACATAATTCTTTCTATAGTTACAGGAAATACTTCTTCTATA ATTCTGATTTTGACTCCCATCCTCCACCATTTACTCATCCACTCATTACCTAAATCTTGG CTTTCTTTCCTATATTGTAAATAATCCATCCAAACTTCTAGCCAGTACTGTCAGGAGGGT TCTTGCTCGAGTGAGCTGTTAATACTATTTTCCACTGACAACTTCTGCACATCGAGGACA CAGTGTATCTGAAGACTCCGCTGTATACTTCCAACAACGGGGGCATTTTTCTTTCGTAGT CGGCATGACAATTACTTTATAGGAAGACTCTTCACGAATATCACCACCTTCTAAGTTGAT GAGGAATTTCCCTTTAAGCTCGATTACATCTGCAGTCATCTCTCGTGGTTCCTGAGCCAG TAAAGTTGACTCAGAAGCCATCATTAATTCATTCAACTGAGAGGTGCTGGAAGTCTCTTC AGACTGCAGCATCTCTATTATCTCAAAAAGCAGTCCAGGTTCTATCACAGTGATAACCTT GTACTCAGCTGCATTTTTGCCAGGNATGCTTCCAAGAAATGAGTTTCGCATTGCACACGC ACTTCTCACAGGTTCTTTCAACCCGGGCTTTTTCCAGATAGAACTAGNCCTAATCCCCCC ATACGGAAAACACCTCTGGGCTTTTATTAAAGGTATGTGCTGGAACACCTTTCANCCCGT GAGGAGAATGGAGCTAAGACGATCTTCCT |
Restriction Sites | NotI-NotI |
ACCN | NM_018060 |
Insert Size | 3410 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_018060.3, NP_060530.3 |
RefSeq Size | 3549 bp |
RefSeq ORF | 3039 bp |
Locus ID | 55699 |
UniProt ID | Q9NSE4 |
Domains | tRNA-synt_1 |
Protein Families | Druggable Genome |
Protein Pathways | Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis |
Gene Summary | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211919 | IARS2 (Myc-DDK-tagged)-Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein |
CNY 10,568.00 |
|
RC211919L3 | Lenti ORF clone of Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 11,970.00 |
|
RC211919L4 | Lenti ORF clone of Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 11,970.00 |
|
RG211919 | IARS2 (tGFP-tagged) - Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein |
CNY 12,168.00 |