IARS2 (NM_018060) Human Untagged Clone

CAT#: SC113746

IARS2 (untagged)-Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein



  "NM_018060" in other vectors (4)

CNY 10,576.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit polyclonal anti-IARS2 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CAGSSS; ILERS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_018060, the custom clone sequence may differ by one or more nucleotides


ATGCGTTGGGGGCTGCGCCCTCGCGGGCCGGGCGCGGCCGCCCTGGCCACTGCCCGAAGTTTGTGGGGGA
CGCCCCGCCTTCCCTGCAGCCCGGGATGGCAAGGGGCGACGAAGAGGCTTCTGGTGCGGTCGGTCTCCGG
GGCCAGTAACCACCAGCCGAACTCGAATAGTGGCAGATACCGGGACACGGTGCTGCTGCCGCAGACGAGC
TTCCCCATGAAGCTGCTGGGCCGCCAGCAGCCGGACACGGAGCTGGAGATCCAGCAGAAATGTGGATTTT
CAGAACTTTATTCATGGCAAAGAGAAAGAAAAGTAAAGACAGAATTTTGCCTTCATGATGGACCTCCTTA
TGCAAACGGTGACCCTCATGTTGGACATGCTTTAAATAAGATTTTGAAAGACATAGCCAATCGATTCCAT
ATGATGAATGGCTCCAAAATACATTTTGTGCCCGGCTGGGATTGTCATGGGTTGCCCATTGAAATAAAAG
TATTATCAGAACTTGGTAGAGAAGCTCAGAATCTTTCAGCTATGGAAATTAGAAAGAAAGCTAGATCATT
TGCTAAAGCAGCCATTGAGAAACAGAAATCAGCATTTATTCGTTGGGGAATAATGGCAGATTGGAATAAT
TGCTACTATACATTTGATGGGAAGTATGAAGCCAAACAGTTGAGAACTTTTTACCAAATGTATGATAAGG
GCTTGGTTTATCGATCTTACAAACCTGTGTTTTGGTCTCCGTCATCTAGGACTGCATTGGCTGAAGCAGA
ACTTGAATATAATCCTGAGCATGTCAGTCGTTCAATATATGTAAAATTTCCTCTCTTAAAGCCTTCTCCA
AAATTGGCATCTCTTATAGATGGTTCATCTCCTGTTAGTATTTTGGTCTGGACCACACAACCTTGGACGA
TTCCAGCCAATGAAGCTGTTTGCTATATGCCTGAATCAAAGTATGCTGTTGTGAAATGTTCTAAGTCTGG
AGACCTCTACGTACTGGCGGCAGATAAAGTAGCATCTGTTGCTTCTACTTTGGAAACAACATTTGAGACT
ATTTCAACACTTTCAGGTGTAGATTTGGAAAATGGTACTTGCAGTCATCCATTAATTCCTGATAAAGCCT
CTCCTCTTTTACCTGCAAATCATGTGACCATGGCAAAAGGAACGGGATTGGTTCACACAGCCCCAGCTCA
TGGTATGGAAGACTACGGTGTAGCGTCTCAGCACAACCTGCCCATGGATTGTCTAGTGGACGAAGATGGA
GTTTTCACAGATGTTGCAGGTCCTGAACTTCAAAACAAGGCTGTCCTTGAAGAGGGAACTGATGTGGTTA
TAAAGATGCTTCAGACTGCAAAGAATTTGTTGAAAGAGGAGAAATTGGTGCATAGCTATCCGTATGACTG
GAGGACCAAGAAACCTGTGGTTATTCGTGCCAGCAAGCAGTGGTTTATAAACATCACGGATATTAAGACT
GCAGCCAAGGAATTGTTAAAAAAGGTGAAATTTATTCCTGGATCAGCACTGAATGGCATGGTTGAAATGA
TGGACAGGCGGCCATATTGGTGTATATCAAGGCAAAGAGTTTGGGGTGTTCCAATTCCTGTGTTTCATCA
TAAGACCAAGGATGAATACTTGATCAACAGCCAAACCACTGAGCATATTGTTAAACTAGTGGAACAACAC
GGCAGTGATATCTGGTGGACTCTTCCCCCTGAACAACTTCTTCCAAAAGAAGTCTTATCTGAGGTTGGTG
GCCCTGATGCCTTGGAATATGTGCCAGGTCAGGATATTTTGGACATCTGGTTTGATAGCGGAACTTCATG
GTCTTATGTTCTTCCAGGTCCTGACCAAAGAGCAGATTTGTACTTGGAAGGAAAAGACCAGCTCGGGGGT
TGGTTTCAGTCATCCTTATTAACAAGTGTGGCAGCAAGGAAGAGAGCACCTTATAAGACAGTGATTGTTC
ATGGATTTACCCTTGGAGAAAAGGGAGAAAAGATGTCCAAGTCTCTTGGGAATGTCATTCATCCTGATGT
TGTCGTTAATGGAGGACAAGATCAAAGCAAAGAGCCTCCGTATGGTGCTGATGTCCTTCGCTGGTGGGTA
GCTGATTCCAATGTCTTCACCGAAGTTGCAATTGGCCCATCCGTGCTCAATGCTGCCAGAGATGATATTA
GCAAGCTTAGGAATACACTTCGCTTTCTTTTGGGAAATGTGGCTGATTTCAACCCAGAAACAGATTCCAT
CCCTGTAAACGATATGTATGTCATAGACCAGTACATGCTACACTTACTGCAGGATTTGGCAAACAAGATT
ACCGAATTATACAAACAATATGATTTTGGAAAAGTTGTTCGGCTGTTACGGACGTTTTATACCAGAGAGC
TCTCTAACTTTTATTTCAGTATAATCAAAGATAGGCTCTATTGTGAAAAGGAAAATGACCCCAAACGACG
CTCTTGTCAGACTGCATTAGTTGAAATTTTGGATGTAATAGTTCGTTCTTTTGCTCCCATTCTTCCTCAC
CTGGCTGAAGAGGTGTTCCAGCACATACCTTATATTAAAGAGCCCAAGAGTGTTTTCCGTACTGGGTGGA
TTAGTACTAGTTCTATCTGGAAAAAGCCCGGGTTGGAAGAAGCTGTGGAGAGTGCGTGTGCAATGCGAGA
CTCATTTCTTGGAAGCATCCCTGGCAAAAATGCAGCTGAGTACAAGGTTATCACTGTGATAGAACCTGGA
CTGCTTTTTGAGATAATAGAGATGCTGCAGTCTGAAGAGACTTCCAGCACCTCTCAGTTGAATGAATTAA
TGATGGCTTCTGAGTCAACTTTACTGGCTCAGGAACCACGAGAGATGACTGCAGATGTAATCGAGCTTAA
AGGGAAATTCCTCATCAACTTAGAAGGTGGTGATATTCGTGAAGAGTCTTCCTATAAAGTAATTGTCATG
CCGACTACGAAAGAAAAATGCCCCCGTTGTTGGAAGTATACAGCGGAGTCTTCAGATACACTGTGTCCTC
GATGTGCAGAAGTTGTCAGTGGAAAATAG


>OriGene 5' read for NM_018060 unedited
AGGGCGGCCACGACATTCGCACGAGGTCTTACTCGGCTCCCCTTGGTTTCCTGGGGTCCT
GCCCCTTCAAGCTGGGGCGGGAGCGGAGGACCCCGCTCTCAGGGGTTGCCGGACCATGCG
TTGGGGGCTGCGCCCTCGCGGGCCGGGCGCGGCCGCCCTGGCCACTGCCCGAAGTTTGTG
GGGGATCGCTCCTCCTTCCCTGCAGCCCGGGATGGCAAGGGGCGACGAAGAGGCTTCTGG
TGCGGTCGGTCTCCGGGGCCAGTAACCACCAGCCGAACTCGAATAGTGGCAGATACCGGG
ACACGGTGCTGCTGCCGCAGACGAGCTTCCCCATGAAGCTGCTGGGCCGCCAGCAGCCGG
ACACGGAGCTGGAGATCCAGCAGAAATGTGGATTTTCAGAACTTTATTCATGGCAAAGAG
AAAGAAAAGTAAAGACAGAATTTTGCCTTCATGATGGACCTCCTTATGCAAACGGTGACC
CTCATGTTGGACATGCTTTAAATAAGATTTTGAAAGACATAGCCAATCGATTCCATATGA
TGAATGGCTCCAAAATACATTTTGTGCCCGGCTGGGATTGTCATGGGTTGCCCATTGAAA
TAAAAGTATTATCAGAACTTGGTAGAGAAGCTCAGAATCTTTCAGCTATGGAAATTAGAA
AGAAAGCTAGATCATTTGCTAAAGCAGCCATTGAGAAACAGAAATCAGCATTTATTCGTT
GGGGAATAATGGCAGATTGGAATAATTGCTACTATACATTTGATGGGAAGTATGAAGCCC
AAACAGTTGAGACTTTTTACCAAATGTATGATAAGGGCTTGGTTTATCGATCTTACAAAC
CTGTGTTTGTCTCCGTCATCTAGGACTGCATTGGCTGAAGCAGAACTG
>OriGene 3' read for NM_018060 unedited
CGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATTTTTCCAGAATGCCTTTAT
TTTCAGTATCATAGAATTTAAATACAGAGTCAAAAGATGATTTATAAAATATAAAACATT
TTCTGCTTGGCCGTATTTGAAGACAAGCTGAATACATATCTATGTTCTGAATAAGTCCAC
TATGGATATATATAGGAAGAGATATACATATATCCATCCACAGATACACACACACATATA
TATTTCTGCATGTATATATACATAATTCTTTCTATAGTTACAGGAAATACTTCTTCTATA
ATTCTGATTTTGACTCCCATCCTCCACCATTTACTCATCCACTCATTACCTAAATCTTGG
CTTTCTTTCCTATATTGTAAATAATCCATCCAAACTTCTAGCCAGTACTGTCAGGAGGGT
TCTTGCTCGAGTGAGCTGTTAATACTATTTTCCACTGACAACTTCTGCACATCGAGGACA
CAGTGTATCTGAAGACTCCGCTGTATACTTCCAACAACGGGGGCATTTTTCTTTCGTAGT
CGGCATGACAATTACTTTATAGGAAGACTCTTCACGAATATCACCACCTTCTAAGTTGAT
GAGGAATTTCCCTTTAAGCTCGATTACATCTGCAGTCATCTCTCGTGGTTCCTGAGCCAG
TAAAGTTGACTCAGAAGCCATCATTAATTCATTCAACTGAGAGGTGCTGGAAGTCTCTTC
AGACTGCAGCATCTCTATTATCTCAAAAAGCAGTCCAGGTTCTATCACAGTGATAACCTT
GTACTCAGCTGCATTTTTGCCAGGNATGCTTCCAAGAAATGAGTTTCGCATTGCACACGC
ACTTCTCACAGGTTCTTTCAACCCGGGCTTTTTCCAGATAGAACTAGNCCTAATCCCCCC
ATACGGAAAACACCTCTGGGCTTTTATTAAAGGTATGTGCTGGAACACCTTTCANCCCGT
GAGGAGAATGGAGCTAAGACGATCTTCCT
Restriction Sites NotI-NotI     
ACCN NM_018060
Insert Size 3410 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_018060.3, NP_060530.3
RefSeq Size 3549 bp
RefSeq ORF 3039 bp
Locus ID 55699
UniProt ID Q9NSE4
Domains tRNA-synt_1
Protein Families Druggable Genome
Protein Pathways Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
Gene Summary Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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