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ZAK (MAP3K20) (NM_016653) Human Untagged Clone
CAT＃: SC114182
ZAK (untagged)-Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1
CNY 8,800.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AZK; CNM6; MLK7; mlklak; MLT; MLTK; MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_016653 edited
ATGTCGTCTCTCGGTGCCTCCTTTGTGCAAATTAAATTTGATGACTTGCAGTTTTTTGAA AACTGCGGTGGAGGAAGTTTTGGGAGTGTTTATCGAGCCAAATGGATATCACAGGACAAG GAGGTGGCTGTAAAGAAGCTCCTCAAAATAGAGAAAGAGGCAGAAATACTCAGTGTCCTC AGTCACAGAAACATCATCCAGTTTTATGGAGTAATTCTTGAACCTCCCAACTATGGCATT GTCACAGAATATGCTTCTCTGGGATCACTCTATGATTACATTAACAGTAACAGAAGTGAG GAGATGGATATGGATCACATTATGACCTGGGCCACTGATGTAGCCAAAGGAATGCATTAT TTACATATGGAGGCTCCTGTCAAGGTGATTCACAGAGACCTCAAGTCAAGAAACGTTGTT ATAGCTGCTGATGGAGTATTGAAGATCTGTGACTTTGGTGCCTCTCGGTTCCATAACCAT ACAACACACATGTCCTTGGTTGGAACTTTCCCATGGATGGCTCCAGAAGTTATCCAGAGT CTCCCTGTGTCAGAAACTTGTGACACATATTCCTATGGTGTGGTTCTCTGGGAGATGCTA ACAAGGGAGGTCCCCTTTAAAGGTTTGGAAGGATTACAAGTAGCTTGGCTTGTAGTGGAA AAAAACGAGAGATTAACCATTCCAAGCAGTTGCCCCAGAAGTTTTGCTGAACTGTTACAT CAGTGTTGGGAAGCTGATGCCAAGAAACGGCCATCATTCAAGCAAATCATTTCAATCCTG GAGTCCATGTCAAATGACACGAGCCTTCCTGACAAGTGTAACTCATTCCTACACAACAAG GCGGAGTGGAGGTGCGAAATTGAGGCAACTCTTGAGAGGCTAAAGAAACTAGAGCGTGAT CTCAGCTTTAAGGAGCAGGAGCTTAAAGAACGAGAAAGACGTTTAAAGATGTGGGAGCAA AAGCTGACAGAGCAGTCCAACACCCCGCTGCTGCCTTCCTTTGAGATTGGTGCATGGACG GAAGACGATGTGTATTGTTGGGTTCAGCAGCTCGTCAGAAAAGGTGACTCTTCAGCAGAG ATGAGTGTATATGCAAGCTTGTTTAAAGAAAACAACATTACAGGGAAGCGGCTGCTGCTG CTGGAGGAAGAAGACCTGAAAGACATGGGCATTGTCTCCAAGGGGCATATCATTCACTTC AAGTCAGCCATTGAGAAATTAACCCATGATTACATAAATTTGTTTCACTTCCCACCACTA ATTAAGGACTCAGGAGGTGAACCTGAAGAAAATGAGGAAAAAATAGTGAACCTGGAACTG GTTTTTGGTTTTCACTTGAAACCAGGAACTGGCCCACAGGATTGTAAGTGGAAAATGTAT ATGGAGATGGATGGGGATGAAATTGCAATAACCTACATAAAAGATGTGACATTCAACACT AACCTACCTGATGCGGAGATTTTAAAGATGACAAAGCCACCATTTGTAATGGAGAAGTGG ATTGTAGGAATAGCAAAAAGTCAGACTGTGGAGTGCACTGTCACATATGAGAGTGATGTT AGAACTCCAAAAAGCACTAAACATGTCCATTCGATTCAGTGGAGTAGAACAAAACCTCAG GATGAAGTGAAAGCAGTCCAACTTGCCATTCAGACATTATTCACCAATTCAGATGGCAAC CCTGGAAGCAGGTCCGACTCAAGTGCTGATTGCCAGTGGTTAGATACTCTGAGGATGCGG CAGATTGCATCCAACACTTCTTTACAGCGTTCCCAGAGCAATCCTATTCTGGGGTCACCG TTCTTCTCACACTTTGATGGCCAGGATTCCTACGCTGCTGCTGTGAGACGGCCCCAGGTG CCCATTAAGTATCAACAGATTACACCTGTGAACCAGTCCAGAAGCTCGTCTCCTACTCAG TATGGACTGACCAAAAACTTCTCTTCCCTACATCTCAACTCTAGGGACAGTGGCTTTTCC AGTGGCAATACTGACACCTCTTCAGAGAGGGGTCGATACTCAGACAGAAGCAGGAACAAA TATGGACGTGGTAGTATATCACTCAATTCTTCTCCTAGAGGAAGATACAGTGGAAAGAGT CAGCATTCCACTCCTTCAAGAGGAAGATACCCTGGAAAGTTCTACAGGGTTTCTCAGTCA GCACTCAATCCTCACCAGTCGCCTGACTTCAAGAGAAGCCCCAGGGACCTCCACCAACCC AACACCATACCAGGGATGCCTTTGCACCCTGAGACTGACTCAAGAGCCAGTGAAGAGGAC AGCAAAGTCAGCGAAGGGGGCTGGACAAAAGTGGAATACCGGAAAAAGCCCCACAGGCCA TCTCCCGCCAAAACCAATAAAGAGAGAGCCAGAGGGGACCACCGTGGATGGAGAAACTTT TGA >OriGene 5' read for NM_016653 unedited
ACTCGNGTTTGTAAACGACTTCTATAGGCGGCCGACGAATTCGCACGAGGGAGAGGCGGA ATGTTCAACTCCTAACTGCGGCGGAAACGTGGGAGCCGCGCGGGCCGCTGTCGTCCCAAC CCCCGCCGCCCTCGTCGCGCGCGGGGCCTCCGCGCCCCCGGCTGCTGCTCACGCCCCGCC CGGGAGCCAGATTTTGTGGAAGTATAATACTTTGTTATTATGAGATGTCGTCTCTCGGTG CCTCCTTTGTGCAAATTAAATTTGATGACTTGCAGTTTTTTGAAAACTGCGGTGGAGGAA GTTTTGGGAGTGTTTATCGAGCCAAATGGATATCACAGGACAAGGAGGTGGCTGTAAAGA AGCTCCTCAAAATAGAGAAAGAGGCAGAAATACTCAGTGTCCTCAGTCACAGAAACATCA TCCAGTTTTATGGAGTAATTCTTGAACCTCCCAACTATGGCATTGTCACAGAATATGCTT CTCTGGGATCACTCTATGATTACATTAACAGTAACAGAAGTGAGGAGATGGATATGGATC ACATTATGACCTGNGCCACTGATGTAGCCAAAGGAATGCATTATTTACATATGGAGGCTC CTGTCAAGGTGATTCACAGAGACCTCAAGTCAAGAAACGTTGTTATAGCTGCTGATGGAG TATTGAAGATCTGTGACTTTGGTGCCTCTCGGTTCCATAACCATACAACACACATGTCCT TGGTTGGAACTTTCCCATGGATGGCNTCAGAAGTTATCCAGAGTCTCCCTGTGTCAGAAA CTTGTGACACATATTCCCTATGGTGTGGTTCTCTGGGAGATGCTAACAAGGGGAGTCCCC TTTAAAGGTTGGNAGGAATACCAGTAGCTTGGCTTGGNNTGGAAAAAACGANAGATTACC CTTTCCAGCAGTTGCCCCAGAATTT >OriGene 3' read for NM_016653 unedited
CCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAACCTGCTTAGAAAACTA TGTAGTTCAATTCATCAAAAGTTTCTCCATCCACGGTGGTCCCCTCTGGCTCTCTCTTTA TTGGTTTTGGCGGGAGATGGCCTGTGGGGCTTTTTCCGGTATTCCACTTTTGTCCAGCCC CCTTCGCTGACTTTGCTGTCCTCTTCACTGGCTCTTGAGTCAGTCTCAGGGTGCAAAGGC ATCCCTGGTATGGTGTTGGGTTGGTGGAGGTCCCTGGGGCTTCTCTTGAAGTCAGGCGAC TGGTGAGGATTGAGTGCTGACTGAGAAACCCTGTAGAACTTTCCAGGGTATCTTCCTCTT GAAGGAGTGGAATGCTGACTCTTTCCACTGTATCTTCCTCTAGGAGAAGAATTGAGTGAT ATACTACCACGTCCATATTTGTTCCTGCTTCTGTCTGAGTATCGACCCCTCTCTGAAGAG GTGTCAGTATTGCCACTGGAAAAGCCACTGTCCCTAGAGTTGAGATGTAGGGAAGAGAAG TTTTTGGTCAGTCCATACTGAGTAGGAGACGAGCTTCTGGACTGGTTCACAGGTGTAATC TGTTGATACTTAATGGGCACCTGGGGCCGTCTCACAGCAGCAGCGTAGGAATCCTGGCCA TCAAAGTGTGAGAAGAACGGTGACCCCAGAATAGGATTGCTCTGGGAACGCTGTANAGAA AGTGTGGGATGCATCTGCCGCATCCTCAGAGTATCTAACCACTGGNCATCAGCACTTGAG TCGGACCTGCTTCCAGGGGTGCCATCTGAATTGGTGAAATATGGCTGAATGGGCAGTTGG ACTGCTTCACTTCTCCTGGAGGGTTTGNTCTACTCACTGATCGATGGACATGNTAGTNGC TTTTGGNAGTCTACATACTCTCATAGTGACGGGCACTCCCAGTCTGACTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016653 |
| Insert Size | 2690 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016653.1, NP_057737.1 |
| RefSeq Size | 3767 bp |
| RefSeq ORF | 2403 bp |
| Locus ID | 51776 |
| UniProt ID | Q9NYL2 |
| Domains | pkinase, TyrKc, SAM, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway, Tight junction |
| Gene Summary | This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1, also referred to as MRK-alpha and MLTK-alpha). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221970 | ZAK (Myc-DDK-tagged)-Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1 |
CNY 8,784.00 |
|
| RC221970L1 | Lenti ORF clone of Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1, Myc-DDK-tagged |
CNY 11,184.00 |
|
| RC221970L2 | Lenti ORF clone of Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1, mGFP tagged |
CNY 11,184.00 |
|
| RC221970L3 | Lenti ORF clone of Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC221970L4 | Lenti ORF clone of Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG221970 | ZAK (tGFP-tagged) - Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1 |
CNY 10,384.00 |
