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LCMT1 (NM_016309) Human Untagged Clone
CAT＃: SC114308
LCMT1 (untagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1
CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGI-68; LCMT; PPMT1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114308 sequence for NM_016309 edited (data generated by NextGen Sequencing)
ATGGCCACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGAC GCAGACGACGAGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCA GTAAGCATTGGCTACTGGCATGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAG AGGAAAGCCCCTGAAATCAACAGAGGATATTTTGCTCGAGTCCATGGTGTCAGTCAGCTT ATAAAGGCATTTCTACGGAAGACAGAATGTCATTGTCAAATTGTCAACCTTGGGGCAGGC ATGGATACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAAATATTTTGAG GTTGACTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTA TCCAGCCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTG GATTCAAAGAGATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAG AAGCTAAAGAAATGTAACATGAATACACAATTGCCAACACTCCTGATAGCTGAATGTGTG CTGGTTTACATGACTCCAGAGCAGTCCGCAAACCTCCTGAAGTGGGCAGCCAACAGTTTT GAGAGAGCCATGTTCATAAACTACGAACAGGTGAACATGGGTGATCGGTTTGGGCAGATC ATGATTGAAAACCTGCGGAGACGCCAGTGTGACCTGGCGGGAGTGGAGACCTGCAAGTCA TTAGAGTCACAGAAAGAACGGCTCCTGTCGAATGGGTGGGAAACAGCATCGGCCGTCGAC ATGATGGAGTTGTACAACAGGTTACCTCGAGCTGAAGTGAGCAGGATAGAATCACTTGAA TTCCTGGATGAAATGGAGCTGCTGGAGCAGCTCATGCGGCATTACTGCCTTTGCTGGGCA ACCAAAGGAGGAAATGAGCTTGGGCTGAAGGAGATAACTTATTAA Clone variation with respect to NM_016309.2 >OriGene 5' read for NM_016309 unedited
GGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTGAGCCAG GTAGGGCCCTACCCTCTTCTGTTGCTTTCTCCCTGTGGCTCGCGCCGTCCCCCGCCGCCC GTCGACCCCGCTTCCATGTCCCTGGCGGACACAGCTCCCAGGAACCTCCACGCCCATGGC CACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGACGCAGA CGACGAGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCAGTAAG CATTGGCTACTGGCATGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAGAGGAA AGCCCCTGAAATCAACAGAGGATATTTTGCTCGAGTCCATGGTGTCAGTCAGCTTATAAA GGCATTTCTACGGAAGACAGAATGTCATTGTCAAATTGTCAACCTTGGGGCAGGCATGGA TACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCAAGTAAATATTTTGAGGTTGA CTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATGCAAGCCTCCCCTATCCAG CCCCATTCTAGAACTGCATTCAGAGGACACACTTCAGATGGATGGACACATACTGGATTC AAAGAGATATGCCGTTATTGGAGCAGATCTCCGAGACCTGTCTGAACTGGAAGAGAAGCT AAAGAAATGTAACATGAATACACAATTGCCAACACTCCTGATAGCTGAATGGTGTGCTGG TTTACATGACTCCAGAGCAGTCCGCANACCTNNCTGAGTGGGCAGCCAACANGTTTGAGA AGACCATGTTCATAAACTACGAACAGGTGAACAGGGGTGGATCGGNTTGGNGCAGATCAT GAATNGAAACCCTGCGAGACGCCAGTGTGACCTGGCGGNNAGTGGAGACCTGCAGTCATT AA >OriGene 3' read for NM_016309 unedited
GACCCCGCCCGAACAACCCGNGCCGCNTCCCCCTTTNNAATTTTTGACTTTGGNCCGCGG NCCGCATACNANGATCGNGTTTTTTTTTTTTTTTTTTGGTGGCAAGTGGAAATTATTTAA ACAACGACATGTCAACAGGGAACAGGAAGAGGAACATGTGCGACCACTGTAGTGACCAAG GCTTCTCAAAAGTGTGGGATGAGACCTGCGGACGAGGCCCGCCCACCGCTCAGGGAGCTT GCAGGTCTCCTCCAGGAGGGCAAGTGGCTTCGGCTTCTGGCTCGGCATAAGCCTTCGACA GATTAATAAGTTATCTCCTTCAGCCCAAGCTCATTTCCTCCTTTGGTTGCCCAGCAAAGG CAGTAATGCCGCATGAGCTGCTCCAGCAGCTCCATTTCATCCAGGAATTCAAGTGATTCT ATCCTGCTCACTTCAGCTCGAGGTAACCTGTTGTACAACTCCATCATGTCGACGGCCGAT GCTGTTTCCCACCCATTCGACAGGAGCCGTTCTTTCTGTGACTCTAATGACTTGCAGGTC TCCACTCCCGCCAGGTCACACTGGCGTCTCCGCAGGTTTTCAATCATGATCTGCCCAAAC CGATCACCCATGTTCACCTGTTCGTAGTTTATGAACATGGCTCTCTCAAAACTGTTGGCT GCCCACTTCAGGAGGTTTGCGGACTGCTCTGGAGTCATGTAAACCAGCACACATTCAGCT ATCAGGAGTGTTGGCAATTGGGTATTCATGTTACATTTCTTTAGCTTCTCTTCCAGTTCA GACAGGTCTCGGAGATCTGCTCCAATAACGCCATATCCTCTTTGAATCCAGTATGTGTGC ATCCATTTGAAGTGTGTCCTCTGGAAGCAAGTTTTAAAATGGGGCTGGTAAGGGGAGGCT TGCCTTTGAACTGGCCAGCTTTTTCGTGAAAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016309 |
| Insert Size | 1380 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016309.2, NP_057393.2 |
| RefSeq Size | 1374 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 51451 |
| UniProt ID | Q9UIC8 |
| Domains | LCM |
| Protein Pathways | Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism |
| Gene Summary | LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Phosphoinositide 3-Kinase Inhibits Cardiac GSK-3 Independently of Akt
,Maradumane L. Mohan, Babal K. Jha, Manveen K. Gupta, Neelakantan T. Vasudevan, Elizabeth E. Martelli, John David Mosinski, and Sathyamangla V. Naga Prasad,
Sci. Signal., Jan 2013; 6: ra4.
[LCMT1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200018 | LCMT1 (Myc-DDK-tagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1 |
CNY 3,656.00 |
|
| RC200018L1 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200018L2 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200018L3 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200018L4 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200018 | LCMT1 (tGFP-tagged) - Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1 |
CNY 5,256.00 |
