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DCDC2 (NM_016356) Human Untagged Clone
CAT＃: SC114336
DCDC2 (untagged)-Human doublecortin domain containing 2 (DCDC2), transcript variant 1
CNY 3,656.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DCDC2A; DFNB66; NPHP19; NSC; RU2; RU2S |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114336 sequence for NM_016356 edited (data generated by NextGen Sequencing)
ATGAGCGGCAGCAGCGCCAGGTCCAGCCACCTGTCTCAGCCCGTCGTGAAGAGCGTGCTT GTGTACCGCAACGGGGACCCCTTCTACGCGGGGCGCCGCGTCGTCATCCATGAGAAGAAG GTGTCCAGCTTCGAAGTCTTCCTGAAGGAGGTGACCGGCGGCGTTCAGGCACCCTTTGGG GCCGTCAGGAACATCTACACCCCGCGGACTGGCCACCGAATCCGGAAGCTAGACCAGATC CAGAGCGGGGGCAATTACGTGGCTGGAGGCCAGGAAGCCTTCAAGAAACTCAATTACTTG GACATAGGAGAAATCAAGAAAAGACCAATGGAAGTTGTTAATACAGAGGTAAAACCAGTA ATCCATAGCAGGATCAACGTGTCAGCTCGCTTTAGAAAACCGCTTCAGGAGCCGTGCACT ATCTTCTTGATTGCAAATGGAGACCTCATAAACCCAGCTTCTCGCCTCCTTATCCCCAGA AAAACCTTGAATCAGTGGGATCATGTACTACAAATGGTCACAGAAAAAATCACTCTGAGG AGCGGGGCTGTTCACAGGCTTTATACTTTAGAAGGAAAACTTGTTGAGAGTGGAGCAGAG TTGGAGAATGGGCAGTTTTATGTGGCTGTTGGCAGAGATAAGTTTAAGAAACTGCCTTAC GGTGAGTTACTTTTTGACAAGTCAACGATGAGAAGGCCTTTTGGTCAGAAAGCTTCTTCA CTACCTCCTATTGTAGGATCCAGAAAGTCTAAAGGGAGTGGAAATGATCGCCACTCTAAG TCAACAGTTGGATCCAGTGACAACTCATCTCCTCAGCCCCTGAAGAGGAAAGGGAAAAAA GAAGACGTGAATTCAGAAAAACTGACGAAATTGAAACAAAATGTAAAATTAAAGAATTCA CAAGAAACCATTCCAAATAGTGATGAAGGCATTTTCAAAGCTGGAGCAGAGAGGTCTGAA ACACGGGGGGCAGCAGAAGTCCAAGAAGATGAAGATACTCAGGTTGAGGTTCCAGTCGAT CAGAGGCCAGCAGAAATAGTAGACGAGGAAGAAGATGGAGAGAAGGCAAACAAGGATGCA GAACAGAAAGAAGACTTTTCAGGAATGAATGGTGACCTTGAAGAGGAAGGAGGTAGGGAG GCTACAGATGCCCCTGAGCAAGTCGAGGAGATTCTGGATCACAGTGAGCAGCAGGCACGC CCTGCTCGTGTAAATGGAGGCACCGATGAGGAGAATGGTGAGGAGCTGCAGCAGGTTAAT AATGAGCTTCAACTGGTCCTAGACAAGGAAAGAAAGTCTCAAGGAGCTGGCAGTGGACAA GATGAGGCTGATGTAGACCCTCAAAGACCACCAAGGCCAGAAGTAAAAATTACCAGTCCA GAAGAAAATGAAAACAACCAACAAAACAAGGACTATGCTGCCGTGGCTTAG Clone variation with respect to NM_016356.3 661 a=>g >OriGene 5' read for NM_016356 unedited
GTGTTCANCATTTTGTNAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGAGC GGAGGTGCCTCCCGACGCGAAGCAGCGAGCTGAGGCGGCGGCCAGCGGGGAAGATGAGCG GCAGCAGCGCCAGGTCCAGCCACCTGTCTCAGCCCGTCGTGAAGAGCGTGCTTGTGTACC GCAACGGGGACCCCTTCTACGCGGGGCGCCGCGTCGTCATCCATGAGAAGAAGGTGTCCA GCTTCGAAGTCTTCCTGAAGGAGGTGACCGGCGGCGTTCAGGCACCCTTTGGGGCCGTCA GGAACATCTACACCCCGCGGACTGGCCACCGAATCCGGAAGCTAGACCAGATCCAGAGCG GGGGCAATTACGTGGCTGGAGGCCAGGAAGCCTTCAAGAAACTCAATTACTTGGACATAG GAGAAATCAAGAAAAGACCAATGGAAGTTGTTAATACAGAGGTAAAACCAGTAATCCATA GCAGGATCAACGTGTCAGCTCGCTTTAGAAAACCGCTTCAGGAGCCGTGCACTATCTTCT TGATTGCAAATGGAGACCTCATAAACCCAGCTTCTCGCCTCCTTATCCCCAGAAAAACCT TGAATCAGTGGGATCATGTACTACAAATGGTCACAGAAAAAATCACTCTGAGGAGCGGGG CTGTTCACAGGCTTTATACTTTAGGAAGAAAACCTTGTGAGAGTGGAGCAGAGTTGGAGA ATGGGCCAGTTTATGTGCCTGTTGGCAGAGATAAGTTTAAGAAACTGCCTTACCGTGAGT TACTTTTTTGACAAGTCAACGATTGAGAAGCCTTTTGGTCAGAAAGCTTCTTCATACCTC CTATTGTAGGACCAGAAAGTCTAAAGGGAGGGGAAATGATCGCCCTTTTAAGTCACAGGT GGGTCCCGTGACAACTCT >OriGene 3' read for NM_016356 unedited
NTTTAGCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTC CTAAACACTTTTATTTTTGTTGAAAACTATTTGAAAATAATTTAATCTTTGGAATAGCCA ATATCAGGTTCTCTAATAACATAAGGGAGAAGAGAGAGATACTATTGGTAAAGATAGACT GGATCAGTGAAAATCCACAGGATTTCCATTATCTGCAAACCATACATCCAGGCTTTATAA TATAACTGGGTGCTCCTTTACAATTCCTTCCTGAGTGTGCGCCATTGGAGCCACAAGCAT GCAAGCCTGAAAGCATTTAAGTGACGGCATGGCGATACTAGTCACTAACACTCTGAACCA ATGCTTCCTCCCACCAAAATGAACGCCTTGCTCTGTCTTTGTAGTTCAAAGGTGGATTTT GGCTAAGGAGGAAGAAAGAAAGGCATGTTAACAAGTCATGGACACATTTTGATCTACTGC CATCTCCAAATTATGTCTGAAAGTAAATCGCAATCTGTTCATTCAAGAGTTTATTGCTTT AAGAAAATAGTACATGCTTTCCTATTACCAGTTCTTTAAAATTCTACGCCTTCCTAGAGA AAAATTATTTAGAATAAACTGCCTTCTAAACTTATAATAAAGCGTACAATAAGAGTGATC CTATTTTTCATCCTTTACAAGTGGCAATTGTCTTCCAGTGCAAATTCTCCTCTGTCCGTC TTATTTATATTTCTATATGACTTTTAAACACATGCATTAAAGTACGTATTATCCTTTAGT AGCCATTTAAAGGTATTTGGTNCTTTCCACTAGCTTTTATGTTATAATTCGGAGTAGTAT TCGACCTAGTGCCCATTATATCAGCATACCTTGTGCTTACTTTCAGAATGATAACCTCTT TTTCTGCGACCATTACTCCTTTTAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016356 |
| Insert Size | 2440 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016356.1, NP_057440.1 |
| RefSeq Size | 2167 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 51473 |
| UniProt ID | Q9UHG0 |
| Domains | DCX |
| Gene Summary | This gene encodes a doublecortin domain-containing family member. The doublecortin domain has been demonstrated to bind tubulin and enhance microtubule polymerization. This family member is thought to function in neuronal migration where it may affect the signaling of primary cilia. Mutations in this gene have been associated with reading disability (RD) type 2, also referred to as developmental dyslexia. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
DCDC2 Mutations Cause a Renal-Hepatic Ciliopathy by Disrupting Wnt Signaling
,null,
American Journal of Human Genetics
,PubMed ID 25557784
[DCDC2]
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Other Versions
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| RC208721 | DCDC2 (Myc-DDK-tagged)-Human doublecortin domain containing 2 (DCDC2), transcript variant 1 |
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| RC208721L1 | Lenti ORF clone of Human doublecortin domain containing 2 (DCDC2), transcript variant 1, Myc-DDK-tagged |
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| RC208721L2 | Lenti ORF clone of Human doublecortin domain containing 2 (DCDC2), transcript variant 1, mGFP tagged |
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| RC208721L3 | Lenti ORF clone of Human doublecortin domain containing 2 (DCDC2), transcript variant 1, Myc-DDK-tagged |
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| RC208721L4 | Lenti ORF clone of Human doublecortin domain containing 2 (DCDC2), transcript variant 1, mGFP tagged |
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| RG208721 | DCDC2 (tGFP-tagged) - Human doublecortin domain containing 2 (DCDC2), transcript variant 1 |
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