United States
Germany
Japan
United Kingdom
China
TRPC4 (NM_016179) Human Untagged Clone
CAT＃: SC114411
TRPC4 (untagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha
CNY 10,744.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HTRP-4; HTRP4; TRP4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC114411 representing NM_016179.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTCAGTTCTATTACAAAAGAAATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTA AGAGCAGAATCAGAACTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCC AGTGTCAAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCTCTC GGAAGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTACTCTTAAGCTTT AATGTCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTCGTCGGAGCTGTTGAGCTGTTA TTGAACCACAAAAAACCTAGTGGAGAAAAACAGGTGCCTCCTATACTCCTTGATAAGCAGTTCTCTGAA TTCACTCCAGACATTACACCAATCATTTTGGCAGCCCATACAAATAATTATGAGATAATAAAACTCTTG GTTCAGAAAGGAGTCTCAGTGCCTCGACCCCACGAGGTCCGCTGTAACTGTGTGGAATGCGTGTCCAGT TCAGATGTGGACAGCCTCCGTCACTCACGCTCCAGACTCAACATCTACAAGGCCTTGGCCAGTCCCTCT CTCATTGCACTGTCAAGCGAAGATCCTTTTCTCACAGCCTTTCAGTTAAGTTGGGAACTTCAGGAACTG AGCAAGGTGGAAAATGAATTCAAGTCGGAGTATGAAGAGCTGTCACGGCAGTGCAAACAATTTGCTAAG GACCTACTGGATCAGACGAGAAGTTCCAGAGAACTGGAAATCATTCTTAATTACCGAGATGACAATAGT CTCATAGAAGAACAAAGTGGAAATGATCTTGCAAGACTAAAATTGGCCATTAAGTACCGTCAAAAAGAG TTTGTTGCCCAGCCCAATTGTCAACAGCTGCTGGCATCTCGCTGGTACGATGAGTTTCCAGGCTGGAGG AGAAGACACTGGGCAGTGAAGATGGTGACATGTTTCATAATAGGACTTCTTTTTCCTGTCTTCTCTGTG TGCTACCTGATAGCTCCCAAAAGCCCACTTGGACTGTTCATCAGGAAGCCATTTATCAAGTTTATCTGC CACACAGCCTCCTATTTGACTTTTTTGTTCCTGCTGCTGCTTGCCTCTCAGCACATCGACAGGTCAGAC TTGAACAGGCAAGGTCCACCACCAACCATCGTCGAGTGGATGATATTACCGTGGGTCCTGGGCTTCATA TGGGGAGAAATTAAACAGATGTGGGATGGCGGACTTCAGGACTACATCCATGATTGGTGGAATCTAATG GACTTTGTAATGAACTCCTTATATTTAGCAACAATCTCCTTGAAAATTGTTGCATTTGTAAAGTACAGT GCCCTTAATCCACGAGAATCATGGGACATGTGGCATCCCACTCTGGTGGCAGAGGCTTTATTTGCTATT GCAAACATCTTCAGTTCTCTGCGTCTGATCTCACTGTTTACTGCAAATTCTCACCTGGGACCTCTGCAA ATATCTCTGGGAAGAATGCTCCTGGACATTTTGAAGTTTCTATTCATATACTGCCTTGTGTTGCTAGCA TTTGCAAATGGCCTAAATCAATTGTACTTCTATTATGAAGAAACGAAAGGGTTAACCTGCAAAGGCATA AGATGTGAAAAGCAGAATAATGCATTTTCAACGTTATTTGAGACACTGCAGTCCCTGTTTTGGTCAATA TTTGGGCTCATCAATTTATATGTGACCAATGTCAAAGCACAGCATGAATTTACTGAGTTTGTTGGTGCC ACCATGTTTGGGACATACAATGTCATCTCTCTGGTTGTTCTACTCAACATGTTAATAGCTATGATGAAT AATTCTTACCAACTGATTGCTGACCATGCAGATATAGAATGGAAATTTGCACGAACAAAGCTTTGGATG AGTTATTTTGAAGAAGGAGGTACTCTGCCTACTCCCTTCAATGTCATCCCGAGCCCCAAGTCTCTCTGG TACCTGATCAAATGGATCTGGACACACTTGTGCAAGAAAAAGATGAGAAGAAAGCCAGAAAGTTTTGGA ACAATAGGGAGGCGAGCTGCTGATAACTTGAGAAGACATCACCAATACCAAGAAGTTATGAGGAACCTG GTGAAGCGATACGTTGCTGCAATGATTAGAGATGCTAAAACTGAAGAAGGCCTGACCGAAGAGAACTTT AAGGAACTAAAGCAAGACATTTCTAGTTTCCGCTTTGAAGTCCTGGGATTACTAAGAGGAAGCAAACTT TCCACAATACAATCTGCGAATGCCTCGAAGGAGTCTTCAAATTCGGCAGACTCAGATGAAAAGAGTGAT AGCGAAGGTAATAGCAAGGACAAGAAAAAGAATTTCAGCCTTTTTGATTTAACCACCCTGATTCATCCG AGATCAGCAGCAATTGCCTCTGAAAGACATAACATAAGCAATGGCTCTGCCCTGGTGGTTCAGGAGCCG CCCAGGGAGAAGCAGAGAAAAGTGAATTTTGTGACCGATATCAAAAACTTTGGGTTATTTCATAGACGA TCAAAACAAAATGCTGCTGAGCAAAATGCAAACCAAATCTTCTCTGTTTCAGAAGAAGTTGCTCGTCAA CAGGCTGCAGGACCACTTGAGAGAAATATTCAACTGGAATCTCGAGGATTAGCTTCACGGGGTGACCTG AGCATTCCCGGTCTCAGTGAACAATGTGTGTTAGTAGACCATAGAGAAAGGAATACGGACACACTGGGG TTACAGGTAGGAAAGAGAGTGTGTCCATTCAAGTCAGAGAAGGTGGTGGTGGAGGACACGGTTCCTATA ATACCAAAGGAGAAACATGCAAAAGAAGAGGACTCTAGTATAGACTATGATCTAAACCTCCCAGACACA GTCACCCACGAAGATTACGTGACCACAAGATTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_016179 |
| Insert Size | 2934 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016179.2 |
| RefSeq Size | 3534 bp |
| RefSeq ORF | 2934 bp |
| Locus ID | 7223 |
| UniProt ID | Q9UBN4 |
| Domains | ANK, ion_trans |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| MW | 112.1 kDa |
| Gene Summary | This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (1, also known as alpha) encodes isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Novel role for TRPC4 in regulation of macroautophagy by a small molecule in vascular endothelial cells
,Zhang, L;Dai, F;Cui, L;Jing, H;Fan, P;Tan, X;Guo, Y;Zhou, G;,
Biochim. Biophys. Acta
,PubMed ID 25476892
[TRPC4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218941 | TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha |
CNY 10,728.00 |
|
| RC218941L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, Myc-DDK-tagged |
CNY 13,128.00 |
|
| RC218941L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, mGFP tagged |
CNY 5,890.00 |
|
| RC218941L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, Myc-DDK-tagged |
CNY 13,128.00 |
|
| RC218941L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, mGFP tagged |
CNY 13,128.00 |
|
| RG218941 | TRPC4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha |
CNY 12,328.00 |
