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GCP4 (TUBGCP4) (NM_014444) Human Untagged Clone
CAT＃: SC115032
TUBGCP4 (untagged)-Human tubulin, gamma complex associated protein 4 (TUBGCP4)
CNY 4,888.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 76P; GCP-4; GCP4; Grip76; MCCRP3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_014444 edited
GAATTCGGCACGAGGGGAAGGGGAAGCACTCCCCTCGTGGTCGCCTGGAGGTGCGCTGGA GGAGGGGGTGACATAACCAGGGACTCGAGGTCCGCCGTGGGAATGATCCACGAACTGCTC TTGGCTCTGAGCGGGTACCCTGGGTCCATTTTCACCTGGAACAAGCGGAGTGGCCTGCAG GTATCGCAGGACTTCCCTTTCCTCCACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGC CGGCTCGGCACAGACTATATTCGCTTCACTGAGTTCATTGAACAGTACACGGGCCATGTG CAACAGCAGGATCACCATCCATCTCAACAGGGCCAAGGTGGGTTACATGGAATCTACCTG CGGGCCTTCTGCACAGGGCTGGATTCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGAT TTGGAACAAGAGTTCCTGGGTGATCCCCATCTCTCCATATCACATGTCAACTACTTCCTA GACCAGTTCCAGCTTCTTTTTCCCTCTGTGATGGTTGTAGTAGAACAAATTAAAAGTCAA AAGATTCATGGTTGTCAAATCCTGGAAACAGTCTACAAACACAGCTGTGGGGGGTTGCCT CCTGTTCGAAGTGCACTGGAAAAAATCCTGGCCGTTTGTCATGGGGTCATGTATAAACAG CTCTCAGCCTGGATGCTCCATGGACTCCTCTTGGACCAGCATGAAGAATTCTTTATCAAA CAGGGGCCATCTTCTGGTAATGTCAGTGCCCAGCCAGAAGAGGACGAGGAGGATCTGGGC ATTGGGGGACTGACAGGAAAACAACTGAGAGAACTGCAGGACTTGCGCCTGATTGAGGAA GAGAACATGCTGGCACCATCTCTGAAGCAGTTTTCCCTACGAGTGGAGATTTTGCCATCC TACATTCCAGTGAGGGTTGCTGAAAAAATCCTATTTGTTGGAGAATCTGTCCAGATGTTT GAGAATCAAAATGTGAACCTGACTAGAAAAGGATCCATTTTGAAAAACCAGGAAGACACT TTTGCTGCAGAGCTGCACCGTCTCAAGCAGCAGCCACTCTTCAGCTTGGTGGACTTTGAA CAGGTGGTGGATCGCATTCGCAGCACTGTGGCTGAGCATCTCTGGAAGTTGATGGTAGAA GAATCCGATTTACTGGGTCAGCTGAAGATCATTAAAGACTTTTACCTTCTGGGACGTGGA GAACTGTTTCAGGCCTTCATTGACACAGCTCAACACATGTTGAAAACACCACCCACTGCA GTAACTGAGCATGATGTGAATGTGGCCTTTCAACAGTCAGCACACAAGGTATTGCTAGAT GATGACAACCTTCTCCCTCTGTTGCACTTGACAATCGAGTATCACGGAAAGGAGCACAAA GCAGATGCTACTCAGGCAAGAGAAGGGCCTTCTCGGGAAACTTCTCCCCGGGAAGCCCCT GCATCTGGCTGGGCAGCCCTAGGTCTTTCCTACAAAGTACAGTGGCCACTACATATTCTC TTCACCCCAGCTGTCCTGGAAAAGTACAATGTTGTTTTTAAGTACTTACTGAGTGTGCGC CGGGTGCAAGCTGAGCTGCAGCACTGCTGGGCCCTACAAATGCAGCGCAAGCACCTCAAG TCGAACCAGACTGATGCAATCAAGTGGCGCCTAAGAAATCACATGGCATTTTTGGTGGAT AATCTTCAGTACTATCTCCAGGTAGATGTGTTGGAGTCTCAGTTCTCCCAGCTGCTTCAT CAGATCAATTCTACCCGAGACTTTGAAAGCATCCGATTGGCTCATGACCACTTCCTGAGC AATTTGCTGGCTCAATCCTTTATCCTATTGAAACCTGTGTTTCACTGCCTGAATGAAATC CTAGATCTCTGTCACAGTTTTTGTTCGCTGGTCAGTCAGAACCTAGGCCCACTGGATGAG CGTGGAGCCGCCCAGCTGAGCATTCTCGTGAAGGGCTTTAGCCGCCAGTCTTCACTCCTG TTCAAGATTCTCTCCAGTGTTCGGAATCATCAGATCAACTCAGATTTGGCTCAACTACTG TTACGACTAGATTATAACAAATACTATACCCAGGCTGGTGGAACTCTGGGCAGTTTCGGG ATGTGAAAATTTCTGGCTCATAAATTGAAATAACAGCCACGTTCCCAAGGTTGTAACAGA AGATTCAAAACATCCCATTCTAGCCACACACAAATAAATATCTGCGGCTTAGTGATAGGA CTCTACCTTTTCTCCTAGAAGCAGTTACTGAACATCCAGGAGTACAACTCCTTCCCATCA TTCCCATGTGGAAGGGTCTCTCCCATCAAGGAGAACATGTGGCATCTCTGATCCTTTACA TTGAGAACATTTGTTGGATATGTTCATTTATTCAATAGTCATTTATTGAGCACCTACTAC GTACCTTGGTACTGTTCAAGCTGTGGGAGATACAGCGGTAAACAAACAATATAGAGCAGA AAGTTAAATATTTTATGGTTCATATGTGAAAAAGTAATTATGTTTATAAATAGACTAACT GCTGGATGTTACCACCAAGTAAGAAAGCAACAGGTAAGATAGGCTTTCTCTCTCCCTATA CCAAGTAATTTATACCTACACAGATTGGGCAATTCTAGCTAATGAAAATATACTTAAAAG TATTTCTTGGGCCGGGCATGGTGGCTCACGCCTGTGGTCCCGGCACTTTGGGAGGCCGGG GCGGGCGGATCGCCTGAGGTCGGGAGTTTGGGACCAGCCTGACCAACATGATGAAACCTC GATTCTGCTGGGAATGCAAGGATTGGCCGGGTGTGGTGGCATGTGCCTGTGGTCCCAGCT GCTCAGGGAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_014444 unedited
TATATTATGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGGGGA AGCACTCCCCTCGTGGTCGGCTGGAGGTGCGCTGGAGGAGGGGGTGACATAACCAGGGAC TCGAGGTCCGCCGTGGGAATGATCCACGAACTGCTCTTGGCTCTGAGCGGGTACCCTGGG TCCATTTTCACCTGGAACAAGCGGAGTGGCCTTTTTGTATCGCAGGACTTCCCTTTCCTG CACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGCCGGCTCGGCACAGACTATATTCGC TTCACTGAGTTCATTGAACAGTACACGGGCCATGTGCAACAGCAGGATCACCATCCATCT CAACAGGGCCAAGGTGGGTTACATGGAATCTACCTGCGGGCCTTCTGCACAGGGCTGGAT TCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGATTTGGAACAAGAGTTCCTGGGTGAT CCCCATCTCTCCATATCACATGTCAACTACTTCCTAGACCAGTTCCAGCTTCTTTTTCCC TCTGTGATGGTTGTAGTAGAACAAATTAAAAGTCAAAAGATTCATGGTTGTCAAATCCTG GAAACAGTCTACAAACACAGCTGTGGGGGGTTGCCTCCTGTTCGAAGTGCACTGGANAAA AATCCTGGCCGTTTGTCATGGGTCATGTATAAACAGCTCTCAGCCTGGATGCTCCATGGA CTCCTCTTGGACCAGCTTGAAGAATTCTTATCAAACAGGGGCCATCTTCTGGTAATGTCA GTGCCCAGCCACGAGAAGACGANGAGGATCTGGGCATTGNNGGACTGACAGGAAAACAAC TGAGAGAACTGCANGACTTGCGCCTGATTGAGGAAGAGACATGCNTGCACCATCTTCTGA GCAGTTTCCCTACAGTGGAGATTTTGCATCCTACATTCAGTGAGGTGCTGAAAAATCCTA TTGTTGGAGATCTGCCACAGGTT >OriGene 3' read for NM_014444 unedited
TGGTTTGATGAGCGGCGATANCGATTTTCGAGTTTTTTTTTTTTTTTTTTCCCTGAGCAG CTGGGACCACAGGCACATGCCACCACACCCGGCCAATCCTTGCATTCCCAGCAGAATCGA GGTTTCATCATGTTGGTCAGGCTGGTCCCAAACTCCCGACCTCAGGCGATCCGCCCGCCC CGGCCTCCCAAAGTGCCGGGACCACAGGCTTTAGCCACCATGCCCGGCCCAAGAAATACT TTTAAGTATATTTTCATTAGCTAGAATTGCCCAATCTGTGTAGGTATAAATTACTTGGTA TAGGGAGAGAGAAAGCCTATCTTACCTGTTGCTTTCTTACTTGGTGGTAACATCCAGCAG TTAGTCTATTTATAAACATAATTACTTTTTCACATATGAACCATAAAATATTTAACTTTC TGCTCTATATTGTTTGTTTACCGCTGTATCTCCCACAGCTTGAACAGTACCAAGGTACGT AGTAGGTGCTCAATAAATGACTATTGAATAAATGAACATATCCCACAAATGTTCTCAATG TAAAGGATCAGAGATGCCACATGTTCTCCTTGATGGGAGAGACCCTTCCCCTGGGAATGA TGGGAAGGATTGTACTCTGGATGTTCAATAACTGCGTTTAGAAAAAAGGTTAAGTCCATC ACTAACCCCCAAATTTTTATTTGTGTGCGGCTTCAATGGGTCGTTTTTAATTTTTTGTTA CAACCTGGGAAACCGGGTTTTTTTTCATTTTTTAACCCAAATATTTCCCATTCGGAAACT CCCCAAATTTCCCCCCCCGGGATAAGCTCTTCGTTTACTTAATCTACACCGATTCGGCCC CAATGGAGTGTGCGCGGGGTTCCCAACACCGGAAAATTTTCAACCGGCGAAAACTGGGGG TAACACCTCATCAAATTGCTCCCTGCGCGCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014444 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014444.2, NP_055259.2 |
| RefSeq Size | 3087 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 27229 |
| UniProt ID | Q9UGJ1 |
| Domains | Spc97_Spc98 |
| Gene Summary | This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015] Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (b) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200872 | TUBGCP4 (Myc-DDK-tagged)-Human tubulin, gamma complex associated protein 4 (TUBGCP4) |
CNY 4,960.00 |
|
| RC200872L3 | Lenti ORF clone of Human tubulin, gamma complex associated protein 4 (TUBGCP4), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC200872L4 | Lenti ORF clone of Human tubulin, gamma complex associated protein 4 (TUBGCP4), mGFP tagged |
CNY 7,700.00 |
|
| RG200872 | TUBGCP4 (tGFP-tagged) - Human tubulin, gamma complex associated protein 4 (TUBGCP4) |
CNY 6,370.00 |
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