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TRAM1 (NM_014294) Human Untagged Clone
CAT＃: SC115096
TRAM1 (untagged)-Human translocation associated membrane protein 1 (TRAM1)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PNAS8; TRAM; TRAMP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115096 sequence for NM_014294 edited (data generated by NextGen Sequencing)
ATGGCGATTCGCAAGAAAAGCACCAAGAGCCCCCCAGTGCTGAGCCACGAATTCGTCCTG CAGAATCACGCGGACATCGTCTCCTGTGTGGCGATGGTCTTCCTGCTGGGGCTCATGTTT GAGATAACGGCAAAAGCTTCTATCATTTTTGTTACTCTTCAGTACAATGTCACCCTCCCA GCAACAGAAGAACAAGCTACTGAATCAGTGTCCCTTTATTACTATGGCATCAAAGATTTG GCTACTGTTTTCTTCTACATGCTAGTGGCGATAATTATTCATGCCGTAATTCAAGAGTAT ATGTTGGATAAAATTAACAGGCGAATGCACTTCTCCAAAACAAAACACAGCAAGTTTAAT GAATCTGGTCAGCTTAGTGCGTTCTACCTTTTTGCCTGTGTTTGGGGCACATTCATTCTC ATCTCTGAAAACTACATCTCAGACCCAACTATCTTATGGAGGGCTTATCCCCATAACCTG ATGACATTTCAAATGAAGTTTTTCTACATATCACAGCTGGCTTACTGGCTTCATGCTTTT CCTGAACTCTACTTCCAGAAAACCAAAAAAGAAGATATTCCTCGTCAGCTTGTCTACATT GGTCTTTACCTCTTCCACATTGCTGGAGCTTACCTTTTGAACTTGAATCATCTAGGACTT GTTCTTCTGGTGCTACATTATTTTGTTGAATTTCTTTTCCACATTTCCCGCCTGTTTTAT TTTAGCAATGAAAAGTATCAGAAAGGATTTTCTCTGTGGGCAGTTCTTTTTGTTTTGGGA AGACTTCTGACTTTAATTCTTTCAGTACTGACTGTTGGTTTTGGCCTTGCAAGAGCAGAA AATCAGAAGCTGGATTTCAGTACTGGAAACTTCAATGTGTTAGCTGTTAGAATCGCTGTT CTGGCATCCATTTGCGTTACTCAGGCATTTATGATGTGGAAGTTCATTAATTTTCAGCTT CGAAGGTGGAGGGAACATTCTGCTTTTCAGGCACCAGCTGTGAAGAAGAAACCAACAGTA ACTAAAGGCAGATCTTCTAAAAAAGGAACAGAAAATGGTGTGAATGGAACATTAACTTCA AATGTAGCAGACTCTCCCCGGAATAAAAAAGAGAAATCTTCATAA Clone variation with respect to NM_014294.5 >OriGene 5' read for NM_014294 unedited
GTGCGCATTTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGTTTTTTTTCC CCCGGGCGGCCCGGCGGCTGCGTACTGGCTGTGGGATGGGAAGTGAAGCCCCAGCGAGCG GCTGCAGCGGGGCCGTGAGGAGCAGCCAGCGGGAGGCGGCGGCGAGTCGGTGAGCAGCTG GGAAGAGCAGAACCGGGGCGGAGCACCTGCAGGCGCGGGCGGCGGCCCCACCATGGCGAT TCGCAAGAAAAGCACCAAGAGCCCCCCAGTGCTGAGCCACGAATTCGTCCTGCAGAATCA CGCGGACATCGTCTCCTGTGTGGCGATGGTCTTCCTGCTGGGGCTCATGTTTGAGATAAC GGCAAAAGCTTCTATCATTTTTGTTACTCTTCAGTACAATGTCACCCTCCCAGCAACAGA AGAACAAGCTACTGAATCAGTGTCCCTTTATTACTATGGCATCAAAGATTTGGCTACTGT TTTCTTCTACATGCTAGTGGCGATAATTATTCATGCCGTAATTCAAGAGTATATGTTGGA TAAAATTAACAGGCGAATGCACTTCTCCAAAACAAAACACAGCAAGTTTAATGAATCTGG TCAGCTTAGTGCGTTCTACCTTTTTGCCTGTGTTTGGGGCACATTCATTCTCATCTCTNG AAACTACATCTCAGACCCAACTATCTTATGAAGGGCTTATCCCCATAACCTGATGACATT TAAATGAAGTTTTTCTACATATCACAGCTGGGTTACTGGCTTCATGCTTTTCCTGAACTC TACTTNCAAAAAACCAAAAAAGAAGATATTCCTCGTCAGCTTGGCTACATTGGTCTTTAC CTCTTNCCCATTGTTGGGCCTACCTTTTTGACTTGAATCATTTAGGACTTGGTTCTTTGG GGCTCCATTATTTTGTTGAATTTCT >OriGene 3' read for NM_014294 unedited
GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAGTAAAAATTTTAT TTTGATTGATTTCTCAATGTATAGTTCAGTATAATGCCAGTTTTTAATGGCAAAAATTTG GTTCCACTGAAACTCCATAATGCTACAGAGAGCTACTACTTTTTCCAGGAAGTAGGTTAA CAGCTAGAAAGAAAAAGGACAATTTCCTAGCAGCATGGCAACTTAAACTGCAGATCTAAT AGGTCTGCAACTTTTACACTAAAAATGGCACAAACAGCTGGTGACACAAGTGAGAAATGG GGAACAAGATGTGAACACTGAAAAGAACAATATATATACTGTAAATATGATGAATAAACC AAATGTAGCTATAAGAATCTTAAAGGATGATTATAGAAAAGGGAACCAAATATTTTCCTC AAATGCTTTTTAGAGCCTTTCTGTAGCGATACAAATATATATATATATATTTATCCAAAA ATATGTTTTATACAGATAAATGTTTCTCAATTAAAGATGGGACCAAAGCTATAGGTATAA CATAAAGCACATTGTCTTTTGTAAGACTATTTATCCACCTGAATTTTCAATTTCTTTAAA GTTAGTGCATTAAATGTTTTCCAAATATAATTACCTGTCAGTCTTTNTATAGATATAAAT CAAGTAGGCATTATGTTTTAAAAGTGTTTGCAGGTTAAACTTTTCTAGATGCTGTGCATA TTAAACTTATTATGAGCCCCATTNAGAATCATTATTAATAAACATATCAAAAAGGGCTAT ATGTCAGATTTTCCTGTTCATAGTAAATATTTTCTGATTTCCAGTTTACAAGTATTGAAA ATGCACAAGAAAGAATTTACTTCACAAGTACTTNTAAGAATATACTTTGATTAATATGTA TGGTAGNAAAACTCCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014294 |
| Insert Size | 2740 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014294.3, NP_055109.1 |
| RefSeq Size | 2808 bp |
| RefSeq ORF | 1125 bp |
| Locus ID | 23471 |
| UniProt ID | Q15629 |
| Domains | LAG1, TLC |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206316 | TRAM1 (Myc-DDK-tagged)-Human translocation associated membrane protein 1 (TRAM1) |
CNY 5,488.00 |
|
| RC206316L1 | Lenti-ORF clone of TRAM1 (Myc-DDK-tagged)-Human translocation associated membrane protein 1 (TRAM1) |
CNY 7,888.00 |
|
| RC206316L2 | Lenti-ORF clone of TRAM1 (mGFP-tagged)-Human translocation associated membrane protein 1 (TRAM1) |
CNY 5,890.00 |
|
| RC206316L3 | Lenti-ORF clone of TRAM1 (Myc-DDK-tagged)-Human translocation associated membrane protein 1 (TRAM1) |
CNY 5,890.00 |
|
| RC206316L4 | Lenti-ORF clone of TRAM1 (mGFP-tagged)-Human translocation associated membrane protein 1 (TRAM1) |
CNY 5,890.00 |
|
| RG206316 | TRAM1 (tGFP-tagged) - Human translocation associated membrane protein 1 (TRAM1) |
CNY 4,370.00 |
