SEC61A (SEC61A1) (NM_013336) Human Untagged Clone
CAT#: SC115289
SEC61A1 (untagged)-Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1)
CNY 5,488.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ADTKD5; HNFJ4; HSEC61; SEC61; SEC61A |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC115289 sequence for NM_013336 edited (data generated by NextGen Sequencing)
ATGGCAATCAAATTTCTGGAAGTCATCAAGCCCTTCTGTGTCATCCTGCCGGAAATTCAG AAGCCAGAGAGGAAGATTCAGTTTAAGGAGAAAGTGCTGTGGACCGCTATCACCCTCTTT ATCTTCTTAGTGTGCTGCCAGATTCCCCTGTTTGGGATCATGTCTTCAGATTCAGCTGAC CCTTTCTATTGGATGAGAGTGATTCTAGCCTCTAACAGAGGCACATTGATGGAGCTAGGG ATCTCTCCTATTGTCACGTCTGGCCTTATAATGCAACTCTTGGCTGGCGCCAAGATAATT GAAGTTGGTGACACCCCAAAAGACCGAGCTCTCTTCAACGGAGCCCAAAAGTTATTTGGC ATGATCATTACTATCGGCCAGTCTATCGTGTATGTGATGACCGGGATGTATGGGGACCCT TCTGAAATGGGTGCTGGAATTTGCCTGCTAATCACCATTCAGCTCTTTGTTGCTGGCTTA ATTGTCCTACTTTTGGATGAACTCCTGCAAAAAGGATATGGCCTTGGCTCTGGTATTTCT CTCTTCATTGCAACTAACATCTGTGAAACCATCGTATGGAAGGCATTCAGCCCCACTACT GTCAACACTGGCCGAGGAATGGAATTTGAAGGTGCTATCATCGCACTTTTCCATCTGCTG GCCACACGCACAGACAAGGTCCGAGCCCTTCGGGAGGCGTTCTACCGCCAGAATCTTCCC AACCTCATGAATCTCATCGCCACCATCTTTGTCTTTGCAGTGGTCATCTATTTCCAGGGC TTCCGAGTGGACCTGCCAATCAAGTCGGCCCGCTACCGTGGCCAGTACAACACCTATCCC ATCAAGCTCTTCTATACGTCCAACATCCCCATCATCCTGCAGTCTGCCCTGGTGTCCAAC CTTTATGTCATCTCCCAAATGCTCTCAGCTCGCTTCAGTGGCAACTTGCTGGTCAGCCTG CTGGGCACCTGGTCGGACACGTCTTCTGGGGGCCCAGCACGTGCTTATCCAGTTGGTGGC CTTTGCTATTACCTGTCCCCTCCAGAATCTTTTGGCTCCGTGTTAGAAGACCCGGTCCAT GCAGTTGTATACATAGTGTTCATGCTGGGCTCCTGTGCATTCTTCTCCAAAACGTGGATT GAGGTCTCAGGTTCCTCTGCCAAAGATGTTGCAAAGCAGCTGAAGGAGCAGCAGATGGTG ATGAGAGGCCACCGAGAGACCTCCATGGTCCATGAACTCAACCGGTACATCCCCACAGCC GCGGCCTTTGGTGGGCTGTGCATCGGGGCCCTCTCGGTCCTGGCTGACTTCCTAGGCGCC ATTGGGTCTGGAACCGGGATCCTGCTCGCAGTCACAATCATCTACCAGTACTTTGAGATC TTCGTTAAGGAGCAAAGCGAGGTTGGCAGCATGGGGGCCCTGCTCTTCTGA Clone variation with respect to NM_013336.3 >OriGene 5' read for NM_013336 unedited
GTTTAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAACGCGCT GGGCCGCGGGCAGCGTCGCCTCACGCGGAGCAGAGCTGAGCTGAAGCGGGACCCGGAGCC CGAGCAGCCGCCGCCATGGCAATCAAATTTCTGGAAGTCATCAAGCCCTTCTGTGTCATC CTGCCGGAAATTCAGAAGCCAGAGAGGAAGATTCAGTTTAAGGAGAAAGTGCTGTGGACC GCTATCACCCTCTTTATCTTCTTAGTGTGCTGCCAGATTCCCCTGTTTGGGATCATGTCT TCAGATTCAGCTGACCCTTTCTATTGGATGAGAGTGATTCTAGCCTCTAACAGAGGCACA TTGATGGAGCTAGGGATCTCTCCTATTGTCACGTCTGGCCTTATAATGCAACTCTTGGCT GGCGCCAAGATAATTGAAGTTGGTGACACCCCAAAAGACCGAGCTCTCTTCAACGGAGCC CAAAAGTTATTTGGCATGATCATTACTATCGGCCAGTCTATCGTGTATGTGATGACCGGG ATGTATGGGGACCCTTCTGAAATGGGTGCTGGAATTTGCCTGCTAATCACCATTCAGCTC TTTGTTGCTGGCTTAATTGTCCTACTTTTGGATGAACTCCTGCAAAAAGGATATGGCCTT GGCTCTGGTATTTCTCTCTTCATTGCAACTAACATCTGTGAAACCATCGTATGGAAGGCA TTCAGCCCCACTACTGTCAACACTGGNCCGAGAATGGAATTTGAAGGTGCTATCATCGCA CTTTTCCATCTGCTGGCCCACGCACAGACANGGTCCGAGCCCTTNNCGGAGCGTTCTACC GCCAGAATCTTCCCACCTCATGAATCTCATCGNCACCCATCTTGTCTTTGCAGGGGCCAT CTATTTCCAGGGCTTCCGAATGGAC >OriGene 3' read for NM_013336 unedited
CGCCCGCTTTTTAGNATCGAGTTTTTTTTTTTTTTTTTTGCCAGTGCCAGGTATAAAAAG CAAAATTTTAAATTGGAAAATGTCTAGCACTTTACACAGTGGAATGAAAGAATACGAAAT TCAAAAACATTATTAAAAGTCCATATGCCGCAGCAGCACGCGCCATGATGAGAGCTCCCC TTCCGAGGCGCTTCTGGAGCAGCTTCCTCAACCTGTCCGGGAGACGGGCTCAGAAGAGCA GGGCCCCCATGCTGCCAACCTCGCTTTGCTCCTTAACGAAGATCTCAAAGTACTGGTAGA TGATTGTGACTGCGAGCAGGATCCCGGTTCCAGACCCAATGGCGCCTAGGAAGTCAGCCA GGACCGAGAGGGCCCCGATGCACAGCCCACCAAAGGCCGCGGCTGTGGGGATGTACCGGT TGAGCTCATGGACCATGGAGGTCTCTCGGTGGCCTCTCATCACCATCTGCTGCTCCTTCA GCTGCTTTGCAACATCTTTGGCAGAGGAACCTGAGACCTCAATCCACGTTTTGGAGAAGA ATGCACAGGAGCCCAGCATGAACACTATGCATACCAACTGCATGGACCGGGTCTTCTAAC ACGGAGCCAAAAGATTCTGGAGGGGACAGGTAATAGCAAAGGCCACCAACTGGATAAGCA CGTGCTGTGCCCCCAGAAGACGTGTTCGACCAGGTGCCCAGCAGGCTGACCACCAAGTTG CCCCTGAAGCGAGCTGATACACCTGGGAAAGACATAAAGGCCGGAACCACGGCAGACTGC CGATGATGGGGATGTTGGACCATATCACAGCTTGATGGGAAAGTGTTGACCTGCCCCGGA AGCGGCCGACTTGTTGGAGGTCCACTCGCAGCCTTGAAAAAAAGACACTGCAAGACAAGA AGCGGCCATGAGATAATGAGTTGGAAGTTTTGCGGGAGACCCCTCCGAGCCTGGACCTGC TGGGGT |
Restriction Sites | NotI-NotI |
ACCN | NM_013336 |
Insert Size | 1790 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_013336.3, NP_037468.1 |
RefSeq Size | 3656 bp |
RefSeq ORF | 1431 bp |
Locus ID | 29927 |
UniProt ID | P61619 |
Domains | secY |
Protein Families | Transmembrane |
Protein Pathways | Vibrio cholerae infection |
Gene Summary | The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222756 | SEC61A1 (Myc-DDK-tagged)-Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1) |
CNY 5,488.00 |
|
RC222756L1 | Lenti ORF clone of Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1), Myc-DDK-tagged |
CNY 7,888.00 |
|
RC222756L2 | Lenti ORF clone of Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1), mGFP tagged |
CNY 5,890.00 |
|
RC222756L3 | Lenti ORF clone of Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1), Myc-DDK-tagged |
CNY 7,888.00 |
|
RC222756L4 | Lenti ORF clone of Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1), mGFP tagged |
CNY 7,888.00 |
|
RG222756 | SEC61A1 (tGFP-tagged) - Human Sec61 alpha 1 subunit (S. cerevisiae) (SEC61A1) |
CNY 7,088.00 |