RACGAP1 (NM_013277) Human Untagged Clone

CAT#: SC115325

RACGAP1 (untagged)-Human Rac GTPase activating protein 1 (RACGAP1), transcript variant 1



  "NM_013277" in other vectors (6)

CNY 4,712.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
RACGAP1 mouse monoclonal antibody,clone OTI3A5
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CYK4; HsCYK-4; ID-GAP; MgcRacGAP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_013277 edited
GAATTCGGCACGAGGGTGGGCGCGGCGCTGCTCTGCCGTTGGGTGAGGCGCGGAGCGAAG
TGAAGGGTGGCCCAGGTGGGGCCAGGCTGACTGAAAAAGATGGATACTATGATGCTGAAT
GTGCGGAATCTGTTTGAGCAGCTTGTGCGCCGGGTGGAGATTCTCAGTGAAGGAAATGAA
GTCCAATTTATCCAGTTGGCGAAGGACTTTGAGGATTTCCGTAAAAAGTGGCAGAGGACT
GACCATGAGCTGGGGAAATACAAGGATCTTTTGATGAAAGCAGAGACTGAGCGAAGTGCT
CTGGATGTTAAGCTGAAGCATGCACGTAATCAGGTGGATGTAGAGATCAAACGGAGACAG
AGAGCTGAGGCTGACTGCGAAAAGCTGGAACGACAGATTCAGCTGATTCGAGAGATGCTC
ATGTGTGACACATCTGGCAGCATTCAACTAAGCGAGGAGCAAAAATCAGCTCTGGCTTTT
CTCAACAGAGGCCAACCATCCAGCAGCAATGCTGGGAACAAAAGACTATCAACCATTGAT
GAATCTGGTTCCATTTTATCAGATATCAGCTTTGACAAGACTGATGAATCACTGGATTGG
GACTCTTCTTTGGTGAAGACTTTCAAACTGAAGAAGAGAGAAAAGAGGCGCTCTACTAGC
CGACAGTTTGTTGATGGTCCCCCTGGACCTGTAAAGAAAACTCGTTCCATTGGCTCTGCA
GTAGACCAGGGGAATGAATCCATAGTTGCAAAAACTACAGTGACTGTTCCCAATGATGGC
GGGCCCATCGAAGCTGTGTCCACTATTGAGACTGTGCCATATTGGACCAGGAGCCGAAGG
AAAACAGGTACTTTACAACCTTGGAACAGTGACTCCACCCTGAACAGCAGGCAGCTGGAG
CCAAGAACTGAGACAGACAGTGTGGGCACGCCACAGAGTAATGGAGGGATGCGCCTGCAT
GACTTTGTTTCTAAGACGGTTATTAAACCTGAATCCTGTGTTCCATGTGGAAAGCGGATA
AAATTTGGCAAATTATCTCTGAAGTGTCGAGACTGTCGTGTGGTCTCTCATCCAGAATGT
CGGGACCGCTGTCCCCTTCCCTGCATTCCTACCCTGATAGGAACACCTGTCAAGATTGGA
GAGGGAATGCTGGCAGACTTTGTGTCCCAGACTTCTCCAATGATCCCCTCCATTGTTGTG
CATTGTGTAAATGAGATTGAGCAAAGAGGTCTGACTGAGACAGGCCTGTATAGGATCTCT
GGCTGTGACCGCACAGTAAAAGAGCTGAAAGAGAAATTCCTCAGAGTGAAAACTGTACCC
CTCCTCAGCAAAGTGGATGATATCCATGCTATCTGTAGCCTTCTAAAAGACTTTCTTCGA
AACCTCAAAGAACCTCTTCTGACCTTTCGCCTTAACAGAGCCTTTATGGAAGCAGCAGAA
ATCACAGATGAAGACAACAGCATAGCTGCCATGTACCAAGCTGTTGGTGAACTGCCCCAG
GCCAACAGGGACACATTAGCTTTCCTCATGATTCACTTGCAGAGAGTGGCTCAGAGTCCA
CATACTAAAATGGATGTTGCCAATCTGGCTAAAGTCTTTGGCCCTACAATAGTGGCCCAT
GCTGTGCCCAATCCAGACCCAGTGACAATGTTACAGGACATCAAGCGTCAACCCAAGGTG
GTTGAGCGCCTGCTTTCCTTGCCTCTGGAGTATTGGAGTCAGTTCATGATGGTGGAGCAA
GAGAACATTGACCCCCTACATGTCATTGAAAACTCAAATGCCTTTTCAACACCACAGACA
CCAGATATTAAAGTGAGTTTACTGGGACCTGTGACCACTCCTGAACATCAGCTTCTCAAG
ACTCCTTCATCTAGTTCCCTGTCACAGAGAGTCCGTTCCACCCTCACCAAGAACACTCCT
AGATTTGGGAGCAAAAGCAAGTCTGCCACTAACCTAGGACGACAAGGCAACTTTTTTGCT
TCTCCAATGCTCAAGTGAAGTCACATCTGCCTGTTACTTCCCAGCATTGACTGACTATAA
GAAAGGACACATCTGTACTCTGCTCTGCAGCCCTCCTGTACTCATTACTACTTTTAGCAT
TCTCCAGGCTTTTACTCAAGTTTAATTGTGCATGAGGGTTTTATTAAAACTATATATATC
TCCCCTTCCTTCTCCTCAAGTCACATAATATCAGCACTTTGTGCTGGTCATTGTTGGGAG
CTTTTAGATGAGACATCTTTCCAGGGGTAGAAGGGTTAGTATGGAATTGGGTTGTGATTC
TTTTTGGGGAAGGGGGTTATTGTXXXXXXXXXXXXXXXXXXCTCGAC
>OriGene 5' read for NM_013277 unedited
TTCGGATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGGTGGGCGCGGC
GCTGCTCTGCCGTTGGGTGAGGCGCGGAGCGAAGTGAAGGGTGGCCCAGGTGGGGCCAGG
CTGACTGAAAAAGATGGATACTATGATGCTGAATGTGCGGAATCTGTTTGAGCAGCTTGT
GCGCCGGGTGGAGATTCTCAGTGAAGGAAATGAAGTCCAATTTATCCAGTTGGCGAAGGA
CTTTGAGGATTTCCGTAAAAAGTGGCAGAGGACTGACCATGAGCTGGGGAAATACAAGGA
TCTTTTGATGAAAGCAGAGACTGAGCGAAGTGCTCTGGATGTTAAGCTGAAGCATGCACG
TAATCAGGTGGATGTAGAGATCAAACGGAGACAGAGAGCTGAGGCTGACTGCGAAAAGCT
GGAACGACAGATTCAGCTGATTCGAGAGATGCTCATGTGTGACACATCTGGCAGCATTCA
ACTAAGCGAGGAGCAAAAATCAGCTCTGGCTTTTCTCAACAGAGGCCAACCATCCAGCAG
CAATGCTGGGAACAAAAGACTATCAACCATTGATGAATCTGGTTCCATTTTATCAGATAT
CAGCTNTGACAAGACTGATGAATCACTGGATTGGGACTCTTCTTTGGTGAAGACTNTCAN
ACTGAAGAAGAGAGAANAGAGGCGCTCTACTAGCCGACAGTTTGTTGATGGTCCCCCTGG
ACCTGTAAAGAAAACTCGTTCCATTGGCTCTGCAGTAGACCCAGGGAATGAATCCATAGN
TGCAAAAACTACAGTGACTGTTCCCAATGATGGCGGCCCCCATCGAACTGTGTCCACTAT
TGAGACTGTGCCATATTGNACCAGNAGCCGAAGGAAACAGGTACTTTACACCTTGGAACA
GTGACTCCACCCCTGACAGAAGCAGCTG
>OriGene 3' read for NM_013277 unedited
TAGAGTCGAGTTTTTTTTTTTTTTTTTTATTTTGAAAAACTATTTACTTTTTTTCCAATA
TTATCCCAAATAGGTGTTTTACAGATAAGGGTCAATACGAAGTCAAACATTCTACAGAAG
AAAATCGTTTTTACAGACATTAAGAATAATTTTAACAGAAGAAAAAGCTCACATCTATCT
AGATGTGGCTATGTTCCATGGGAAAAATTTCAGCATCCAAAGTGCAAAGAAAAAATGACT
GTAGCTTTTCTTACCACAAAATATTGACAATCTTCCCTTATAGCCTACTCTTTATTGTTA
GTTGGGATGCCAAAGGATGATATATTGACCTTTAGAAGTTGGGCTCCACTGGACAAGGTT
GGGGGTATGGGGGCCAAGCATCAGAATGAATTCAATTTTAAAAGAAAAACTGGCTTTGAC
CCCAAATGAACCCAAAGTTCAGCCAGCGGCACATCAGAGATAAATACGAGTTGTACTTTC
ACATTTACAAGGTTGTGCCACTCAACACTATTAAAGACCTAATCATCCAAATCAAAGCTA
CGCATACTCCCATCACTAGTTCTGGTCTCAATGCATCCCATTTTTATGAAAGCTCCATAT
TATAGCTCAGTTCATAATGGGGAATGAGACCTTCTGGTTCTTTAGAGGAGGCATCCTAAA
TCCTCTGGTCTATCCTTTCTTTGCCCTGCCCTCCCACTCCCACCCTGAGAAAGCTAATCT
TATCAGATTAGTAGGGTTTCTGCACTGNCTCCCGAAAATCAGTCTAAATGAACCTAAAGA
ACGATCTTCTTTGACCCGTTTTTGGCTTTAACCACAGGACCTTACCCCCTTTCCCCAAAG
AATCCCACCAATTCCTCTTACCCCTTTACCCTTGAAAGAAGTTCATTAAAGCCCCCAAAT
GACACCCAAATGCTGATTTTTGGGATTGGGGAAAGGAGGGGAAATTATTAGTTTAATAAC
CCCTGCCCAATAACTGGTAAACCGCGAAAGCTCAATTTTAGGTACCGAGGCGCAACAACC
AAGGTGCCTTTTAAACCAAGTGGG
Restriction Sites NotI-NotI     
ACCN NM_013277
Insert Size 3570 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013277.2, NP_037409.2
RefSeq Size 3237 bp
RefSeq ORF 1899 bp
Locus ID 29127
UniProt ID Q9H0H5
Domains RhoGAP, DAG_PE-bind
Protein Families Druggable Genome
Gene Summary This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]
Transcript Variant: This variant (1) is the reference variant. Variants 1, 2, 3, 4, 5, 6, 7, 8, and 9 encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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