United States
Germany
Japan
United Kingdom
China
STAT5B (NM_012448) Human Untagged Clone
CAT＃: SC115355
STAT5B (untagged)-Human signal transducer and activator of transcription 5B (STAT5B)
CNY 8,656.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GHISID2; STAT5 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115355 sequence for NM_012448 edited (data generated by NextGen Sequencing)
ATGGCTGTGTGGATACAAGCTCAGCAGCTCCAAGGAGAAGCCCTTCATCAGATGCAAGCG TTATATGGCCAGCATTTTCCCATTGAGGTGCGGCATTATTTATCCCAGTGGATTGAAAGC CAAGCATGGGACTCAGTAGATCTTGATAATCCACAGGAGAACATTAAGGCCACCCAGCTC CTGGAGGGCCTGGTGCAGGAGCTGCAGAAGAAGGCAGAGCACCAGGTGGGGGAAGATGGG TTTTTACTGAAGATCAAGCTGGGGCACTATGCCACACAGCTCCAGAACACGTATGACCGC TGCCCCATGGAGCTGGTCCGCTGCATCCGCCATATATTGTACAATGAACAGAGGTTGGTC CGAGAAGCCAACAATGGTAGCTCTCCAGCTGGAAGCCTTGCTGATGCCATGTCCCAGAAA CACCTCCAGATCAACCAGACGTTTGAGGAGCTGCGACTGGTCACGCAGGACACAGAGAAT GAGTTAAAAAAGCTGCAGCAGACTCAGGAGTACTTCATCATCCAGTACCAGGAGAGCCTG AGGATCCAAGCTCAGTTTGGCCCGCTGGCCCAGCTGAGCCCCCAGGAGCGTCTGAGCCGG GAGACGGCCCTCCAGCAGAAGCAGGTGTCTCTGGAGGCCTGGTTGCAGCGTGAGGCACAG ACACTGCAGCAGTACCGCGTGGAGCTGGCCGAGAAGCACCAGAAGACCCTGCAGCTGCTG CGGAAGCAGCAGACCATCATCCTGGATGACGAGCTGATCCAGTGGAAGCGGCGGCAGCAG CTGGCCGGGAACGGCGGGCCCCCCGAGGGCAGCCTGGACGTGCTACAGTCCTGGTGTGAG AAGTTGGCCGAGATCATCTGGCAGAACCGGCAGCAGATCCGCAGGGCTGAGCACCTCTGC CAGCAGCTGCCCATCCCCGGCCCAGTGGAGGAGATGCTGGCCGAGGTCAACGCCACCATC ACGGACATTATCTCAGCCCTGGTGACCAGCACGTTCATCATTGAGAAGCAGCCTCCTCAG GTCCTGAAGACCCAGACCAAGTTTGCAGCCACTGTGCGCCTGCTGGTGGGCGGGAAGCTG AACGTGCACATGAACCCCCCCCAGGTGAAGGCCACCATCATCAGTGAGCAGCAGGCCAAG TCTCTGCTCAAGAACGAGAACACCCGCAATGATTACAGTGGCGAGATCTTGAACAACTGC TGCGTCATGGAGTACCACCAAGCCACAGGCACCCTTAGTGCCCACTTCAGGAATATGTCC CTGAAACGAATTAAGAGGTCAGACCGTCGTGGGGCAGAGTCGGTGACAGAAGAAAAATTT ACAATCTTGTTTGAATCCCAGTTCAGTGTTGGTGGAAATGAGCTGGTTTTTCAAGTCAAG ACCCTGTCCCTGCCAGTGGTGGTGATCGTTCATGGCAGCCAGGACAACAATGCGACGGCC ACTGTTCTCTGGGACAATGCTTTTGCAGAGCCTGGCAGGGTGCCATTTGCCGTGCCTGAC AAAGTGCTGTGGCCACAGCTGTGTGAGGCGCTCAACATGAAATTCAAGGCCGAAGTGCAG AGCAACCGGGGCCTGACCAAGGAGAACCTCGTGTTCCTGGCGCAGAAACTGTTCAACAAC AGCAGCAGCCACCTGGAGGACTACAGTGGCCTGTCTGTGTCCTGGTCCCAGTTCAACAGG GAGAATTTACCAGGACGGAATTACACTTTCTGGCAATGGTTTGACGGTGTGATGGAAGTG TTAAAAAAACATCTCAAGCCTCATTGGAATGATGGGGCCATTTTGGGGTTTGTAAACAAG CAACAGGCCCATGACCTACTCATTAACAAGCCAGATGGGACCTTCCTCCTGAGATTCAGT GACTCAGAAATTGGCGGCATCACCATTGCTTGGAAGTTTGATTCTCAGGAAAGAATGTTT TGGAATCTGATGCCTTTTACCACCAGAGACTTCTCCATTCGGTCCCTAGCCGACCGCTTG GGAGACTTGAATTACCTTATCTACGTGTTTCCTGATCGGCCAAAAGATGAAGTATACTCC AAATACTACACACCAGTTCCCTGCGAGTCTGCTACTGCTAAAGCTGTTGATGGATACGTG AAGCCACAGATCAAGCAAGTGGTCCCTGAGTTTGTGAACGCATCTGCAGATGCCGGGGGC GGCAGCGCCACGTACATGGACCAGGCCCCCTCCCCAGCTGTGTGTCCCCAGGCTCACTAT AACATGTACCCACAGAACCCTGACTCAGTCCTTGACACCGATGGGGACTTCGATCTGGAG GACACAATGGACGTAGCGCGGCGTGTGGAGGAGCTCCTGGGCCGGCCAATGGACAGTCAG TGGATCCCGCACGCACAATCGTGA Clone variation with respect to NM_012448.3 1327 c=>t >OriGene 5' read for NM_012448 unedited
TATTTCCCCGCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC GAATTCGGCACGAGGGTCACCCCGGGCGGGGACCCAGCGCAGGCAACTCCGCGCGGCGGC CCGGCCGAGGGAGGGAGCGAGCGGGCGGGCGGGCAAGCCAGACAGCTGGGCCGGAGCAGC CGCGGGCGCCCGAGGGGCCGAGCGAGATTGTAAACCATGGCTGTGTGGATACAAGCTCAG CAGCTCCAAGGAGAAGCCCTTCATCAGATGCAAGCGTTATATGGCCAGCATTTTCCCATT GAGGTGCGGCATTATTTATCCCAGTGGATTGAAAGCCAAGCATGGGACTCAGTAGATCTT GATAATCCACAGGAGAACATTAAGGCCACCCAGCTCCTGGAGGGCCTGGTGCAGGAGCTG CAGAAGAAGGCAGAGCACCAGGTGGGGGAAGATGGGTTTTTACTGAAGATCAAGCTGGGG CACTATGCCACACAGCTCCAGAACACGTATGACCGCTGCCCCATGGAGCTGGTCCGCTGC ATCCGCCATATATTGTACAATGAACAGAGGTTGGTCCGAGAAGCCAACAATGGTAGCTCT CCAGCTGGAAGCCTTGCTGATGCCATGTCCCAGAAACACCTCCAGATCAACCAGACGTTT GAGGAGCTGCGACTGGTCACGCACGACACAGAGAATGAGTTAAAAAAGCTGCAGCAGACT CAGGAGTACTTCATCTTCCAGTACCAGGAGAGCCTGAGGATCCCAGCTCAGTTTGGCCCG CTGCCCCACTGAGCCCCCAGAGCGTCTGACCGGGAGACGGCCCTCCACAGAAACAGTGTC TCTTGGAGCCTCGTTGCACCGTGAGCACANAACTG >OriGene 3' read for NM_012448 unedited
NNCCGAAATCTATGTCCGCGGCCGCAATCNANGATCGGTTTTTTTTTTTTTTTTTTAACA CTTCACATTATGAGTATTGTTTCAAAANAGAAGCGATTCATGGAATTAAAACATCCACAA GAGTGATTCCTCTGGTGAAGATGAAGAAGCTGAAGATGGAGAGGTCGCGGGGTCACGATT GTGCGTGCGGGATCCACTGACTGTCCATTGGCCGGCCCAGGAGCTCCTCCACACGCCGCG CTACGTCCATTGTGTCCTCCAGATCGAAGTCCCCATCGGTGTCAAGGACTGAGTCAGGGT TCTGTGGGTACATGTTATAGTGAGCCTGGGGACACACAGCTGGGGAGGGGGCCTGGTCCA TGTACGTGGCGCTGCCGCCCCCGGCATCTGCAGATGCGTTCACAAACTCAGGGACCACTT GCTTGATCTGTGGCTTCACGTATCCATCAACAGCTTTAGCAGTAGCAGACTCGCAGGGAA CTGGTGTGTAGTATTTGGAGTATACTTCATCTTTTGGCCGATCAGGAAACACGTAGATAA GGTAATTCAAGTCTCCCAAGCGGTCGGCTAGGGACCGAATGGAGAAGTCTCTGGTGGTAA AAGGCATCAGATTCCAAAACATTCTTTCCTGAGAATCAAACTTCCAAGCAATGGTGATGC CGCCAATTTCTGAGTCACTGAATCTCAGGAGGAAAGTCCCATCTGGCTTGGTAATGAGTA GGTCATGGGCCTGTTGCTTGGTTACCAACCCCAAATGGCCCCATCATTTCAATGAGGGCT GGAGAGTTTTTTTTACACTTTCCTCACACCGGCGAACCATGTCCGAAAGTGTAATTCCGT CCTGGGGAATTCTTCCCGTTGGACTGGGACCAGGAACAAACAGGGCCCTGTAGTCCTCC |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_012448 |
| Insert Size | 2750 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012448.3, NP_036580.2 |
| RefSeq Size | 5171 bp |
| RefSeq ORF | 2364 bp |
| Locus ID | 6777 |
| UniProt ID | P51692 |
| Domains | SH2, STAT |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Jak-STAT signaling pathway, Pathways in cancer |
| Gene Summary | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Discovery of somatic STAT5b mutations in large granular lymphocytic leukemia
,Hanna L. M. Rajala, Samuli Eldfors, Heikki Kuusanmäki, Arjan J. van Adrichem, Thomas Olson, Sonja Lagström, Emma I. Andersson, Andres Jerez, Michael J. Clemente, Yiyi Yan, Dan Zhang, Andy Awwad, Pekka Ellonen, Olli Kallioniemi, Krister Wennerberg, Kimmo Porkka, Jaroslaw P. Maciejewski, Thomas P. Loughran, Jr, Caroline Heckman, and Satu Mustjoki,
Blood, May 2013; 121: 4541 - 4550.
,PubMed ID 23596048
[STAT5B]
|
|
Signal Transducer and Activator of Transcription 5b (Stat5b) Serine 193 Is a Novel Cytokine-induced Phospho-regulatory Site That Is Constitutively Activated in Primary Hematopoietic Malignancies
,Abhisek Mitra, Jeremy A. Ross, Georgialina Rodriguez, Zsuzsanna S. Nagy, Harry L. Wilson, and Robert A. Kirken,
J. Biol. Chem., May 2012; 287: 16596 - 16608.
[STAT5B]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209429 | STAT5B (Myc-DDK-tagged)-Human signal transducer and activator of transcription 5B (STAT5B) |
CNY 8,648.00 |
|
| RC209429L1 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), Myc-DDK-tagged |
CNY 11,048.00 |
|
| RC209429L2 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), mGFP tagged |
CNY 7,700.00 |
|
| RC209429L3 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), Myc-DDK-tagged |
CNY 11,048.00 |
|
| RC209429L4 | Lenti ORF clone of Human signal transducer and activator of transcription 5B (STAT5B), mGFP tagged |
CNY 11,048.00 |
|
| RG209429 | STAT5B (tGFP-tagged) - Human signal transducer and activator of transcription 5B (STAT5B) |
CNY 10,248.00 |
