BTK (NM_000061) Human Untagged Clone

CAT#: SC115442

BTK (untagged)-Human Bruton agammaglobulinemia tyrosine kinase (BTK)



  "NM_000061" in other vectors (7)

CNY 7,220.00


货期*
3周

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
Anti-BTK mouse monoclonal antibody, clone OTI3A2 (formerly 3A2)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AGMX1; AT; ATK; BPK; IGHD3; IMD1; PSCTK1; XLA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000061 edited
ATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACA
TCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTAT
GAGTATGACTTTGAACGTGGGAGAAGAGGCAGTAAGAAGGGTTCAATAGATGTTGAGAAG
ATCACTTGTGTTGAAACAGTGGTTCCTGAAAAAAATCCTCCTCCAGAAAGACAGATTCCG
AGAAGAGGTGAAGAGTCCAGTGAAATGGAGCAAATTTCAATCATTGAAAGGTTCCCTTAT
CCCTTCCAGGTTGTATATGATGAAGGGCCTCTCTACGTCTTCTCCCCAACTGAAGAACTA
AGGAAGCGGTGGATTCACCAGCTCAAAAACGTAATCCGGTACAACAGTGATCTGGTTCAG
AAATATCACCCTTGCTTCTGGATCGATGGGCAGTATCTCTGCTGCTCTCAGACAGCCAAA
AATGCTATGGGCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGGAGTTCT
CACCGGAAGACAAAAAAGCCTCTTCCCCCAACGCCTGAGGAGGACCAGATCTTGAAAAAG
CCACTACCGCCTGAGCCAGCAGCAGCACCAGTCTCCACAAGTGAGCTGAAAAAGGTTGTG
GCCCTTTATGATTACATGCCAATGAATGCAAATGATCTACAGCTGCGGAAGGGTGATGAA
TATTTTATCTTGGAGGAAAGCAACTTACCATGGTGGAGAGCACGAGATAAAAATGGGCAG
GAAGGCTACATTCCTAGTAACTATGTCACTGAAGCAGAAGACTCCATAGAAATGTATGAG
TGGTATTCCAAACACATGACTCGGAGTCAGGCTGAGCAACTGCTAAAGCAAGAGGGGAAA
GAAGGAGGTTTCATTGTCAGAGACTCCAGCAAAGCTGGCAAATATACAGTGTCTGTGTTT
GCTAAATCCACAGGGGACCCTCAAGGGGTGATACGTCATTATGTTGTGTGTTCCACACCT
CAGAGCCAGTATTACCTGGCTGAGAAGCACCTTTTCAGCACCATCCCTGAGCTCATTAAC
TACCATCAGCACAACTCTGCAGGACTCATATCCAGGCTCAAATATCCAGTGTCTCAACAA
AACAAGAATGCACCTTCCACTGCAGGCCTGGGATACGGATCATGGGAAATTGATCCAAAG
GACCTGACCTTCTTGAAGGAGCTGGGGACTGGACAATTTGGGGTAGTGAAGTATGGGAAA
TGGAGAGGCCAGTACGACGTGGCCATCAAGATGATCAAAGAAGGCTCCATGTCTGAAGAT
GAATTCATTGAAGAAGCCAAAGTCATGATGAATCTTTCCCATGAGAAGCTGGTGCAGTTG
TATGGCGTCTGCACCAAGCAGCGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGC
TGCCTCCTGAACTACCTGAGGGAGATGCGCCACCGCTTCCAGACTCAGCAGCTGCTAGAG
ATGTGCAAGGATGTCTGTGAAGCCATGGAATACCTGGAGTCAAAGCAGTTCCTTCACCGA
GACCTGGCAGCTCGAAACTGTTTGGTAAACGATCAAGGAGTTGTTAAAGTATCTGATTTC
GGCCTGTCCAGGTATGTCCTGGATGATGAATACACAAGCTCAGTAGGCTCCAAATTTCCA
GTCCGGTGGTCCCCACCGGAAGTCCTGATGTATAGCAAGTTCAGCAGCAAATCTGACATT
TGGGCTTTTGGGGTTTTGATGTGGGAAATTTACTCCCTGGGGAAGATGCCATATGAGAGA
TTTACTAACAGTGAGACTGCTGAACACATTGCCCAAGGCCTACGTCTCTACAGGCCTCAT
CTGGCTTCAGAGAAGGTATATACCATCATGTACAGTTGCTGGCATGAGAAAGCAGATGAG
CGTCCCACTTTCAAAATTCTTCTGAGCAATATTCTAGATGTCATGGATGAAGAATCCTGA
>OriGene 5' read for NM_000061 unedited
TTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCAGGGCCAGT
GTCTGCTGCGATCGAGTCCCACCTTCCAAGTCCTGGCATCTCAATGCATCTGGGAAGCTA
CCTGCATTAAGTCAGGACTGAGCACACAGGTGAACTCCAGAAAGAAGAAGCTATGGCCGC
AGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCTCT
AAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGA
CTTTGAACGTGGGAGAAGAGGCAGTAAGAAGGGTTCAATAGATGTTGAGAAGATCACTTG
TGTTGAAACAGTGGTTCCTGAAAAAAATCCTCCTCCAGAAAGACAGATTCCGAGAAGAGG
TGAAGAGTCCAGTGAAATGGAGCAAATTTCAATCATTGAAAGGTTCCCTTATCCCTTCCA
GGTTGTATATGATGAAGGGCCTCTCTACGTCTTCTCCCCAACTGAAGAACTAAGGAAGCG
GTGGATTCACCAGCTCAAAAACGTAATCCGGTACAACAGTGATCTGGTTCAGAAATATCA
CCCTTGCTTCTGGATCGATGGGCAGTATCTCTGCTGCTCTCAGACAGCCAAAAATGCTAT
GGGCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTANNACCTGGNGAGTCTCACCGGAA
GACANAAAAGCCTCTTCCCCCAACGCCTNGAGAGGACCAGATCTTTGAAAGCCACTACCG
NCTGAGCCAGCAGCAGCACCAGTCTCCACCAGTGAGCTGAAAAAGTTGTGGCCCCTTATG
ATTACATGCCCNATGATGCANATGATCTACAGCTGCCGGAGGGTGATGAATATTTATCTT
GGNAGAAAAGCACTTACATGGTGGAGAGCCCGAGAAAA
>OriGene 3' read for NM_000061 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAGCTTTCTAGTAATTTTATTT
TATCAAAACACCCTCCCCTCCCATCTTTATGACACCATTTCATTCTTGCCAAATTCGGTC
CACCCCCACACACACACCCCCAAGCTTTACCAAATGCCTACTCAAAGAGCTGTTGGACCC
CTTTGTGCGGCTATTTACATCCTCCCTCCTAAAAAATATTTTCATCGCAAATTCAGTCTG
TCTTAATTCTCTCGGGAAATTTCAGGCACAATAATTTCTTGGCCTTTGCTCAAAAGCCAC
TATCCCAGGGAATCAAAGAAGAGGTGCATTCCCAGATGTAAAGAGGGGCCTTTGTGTATT
GAGTGGGAGCACAAAGGCTCCAGGGCTCCTAAGCTTGGGATTTCCTCTGAGAAAGTGAAA
TTGGGGCTTGTGGAGAAGAGAAGTANAACCAAGAAGCTTATTGGCGAGCTCAGGATTCTT
CATCCATGACATCTAGAATATTGCTCAGAAGAATTTTGAAAGTGGGACGCTCATCTGCTT
TCTCATGCCAGCAACTGTACATGATGGTATATACCTTCTCTGAAGCCAGATGAGGCCTGT
ANAGACGTAGGCCTTGGGCAATGTGTTCAGCAGTCTCACTGTTAGTAAAATCTCTATATG
GCATCTTCCCCAGGGAGTAAATTTCCCCACATAAAACCCCAAAAGCCCAAAGCCAGATTT
GCTGCTGAACTTGTATACATCAGGACTTCCGTGGGGACCACCGGACTGGAAATTTGGACG
CTACTGAGCTTGGGTATTCACATCCCGGACACTCCTGCACAGCGCGAAATAGATACTTTT
ACACTTCCTTGACCGTTAACAAACAAGTTCAACCGTCAAGCCTCGTGGAGGGACCGCTTC
CACCTCCGGTTCCCAGGGTTTCCACCATCCTTGCCCACCTATCATCGGCTGCCTCGGAGC
GGGGCCGCTCCCCTCAGCAGCT
Restriction Sites NotI-NotI     
ACCN NM_000061
Insert Size 2790 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000061.1, NP_000052.1
RefSeq Size 2591 bp
RefSeq ORF 1980 bp
Locus ID 695
UniProt ID Q06187
Domains pkinase, SH2, TyrKc, SH3, BTK, PH, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency
Gene Summary The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]
Transcript Variant: This variant (1) encodes isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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