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Frizzled 4 (FZD4) (NM_012193) Human Untagged Clone
CAT＃: SC115479
FZD4 (untagged)-Human frizzled family receptor 4 (FZD4)
CNY 6,008.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD344; EVR1; FEVR; Fz-4; Fz4; FZD4S; FzE4; GPCR; hFz4 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_012193 edited
GAATTCGGCACGAGGCGGACGCGGCCGGCGCCGGCTTGTGGGCTCGCCGCCTGCAGCCAT GACCCTCGCAGCCTGTCCCTCGGCCTCGGCCCGGGACGTCTAAAATCCCACACAGTCGCG CGCAGCTGCTGGAGAGCCGGCCGCTGCCCCCTCGTCGCCGCATCACACTCCCGTCCCGGG AGCTGGGAGCAGCGCGGGCAGCCGGCGCCCCCGTGCAAACTGGGGGTGTCTGCCAGAGCA GCCCCAGCCGCTGCCGCTGCTACCCCCGATGCTGGCCATGGCCTGGCGGGGCGCAGGGCC GAGCGTCCCGGGGGCGCCCGGGGGCGTCGGTCTCAGTCTGGGGTTGCTCCTGCAGTTGCT GCTGCTCCTGGGGCCGGCGCGGGGCTTCGGGGACGAGGAAGAGCGGCGCTGCGACCCCAT CCGCATCTCCATGTGCCAGAACCTCGGCTACAACGTGACCAAGATGCCCAACCTGGTTGG GCACGAGCTGCAGACGGACGCCGAGCTGCAGCTGACAACTTTCACACCGCTCATCCAGTA CGGCTGCTCCAGCCAGCTGCAGTTCTTCCTTTGTTCTGTTTATGTGCCAATGTGCACAGA GAAGATCAACATCCCCATTGGCCCATGCGGCGGCATGTGTCTTTCAGTCAAGAGACGCTG TGAACCCGTCCTGAAGGAATTTGGATTTGCCTGGCCAGAGAGTCTGAACTGCAGCAAATT CCCACCACAGAACGACCACAACCACATGTGCATGGAAGGGCCAGGTGATGAAGAGGTGCC CTTACCTCACAAAACCCCCATCCAGCCTGGGGAAGAGTGTCACTCTGTGGGAACCAATTC TGATCAGTACATCTGGGTGAAAAGGAGCCTGAACTGTGTGCTCAAGTGTGGCTATGATGC TGGCTTATACAGCCGCTCAGCCAAGGAGTTCACTGATATCTGGATGGCTGTGTGGGCCAG CCTGTGTTTCATCTCCACTGCCTTCACAGTACTGACCTTCCTGATCGATTCTTCTAGGTT TTCCTACCCTGAGCGCCCCATCATATTTCTCAGTATGTGCTATAATATTTATAGCATTGC TTATATTGTCAGGCTGACTGTAGGCCGGGAAAGGATATCCTGTGATTTTGAAGAGGCAGC AGAACCTGTTCTCATCCAAGAAGGACTTAAGAACACAGGATGTGCAATAATTTTCTTGCT GATGTACTTTTTTGGAATGGCCAGCTCCATTTGGTGGGTTATTCTGACACTCACTTGGTT TTTGGCAGCAGGACTCAAATGGGGTCATGAAGCCATTGAAATGCACAGCTCTTATTTCCA CATTGCAGCCTGGGCCATCCCCGCAGTGAAAACCATTGTCATCTTGATTATGAGACTGGT GGATGCAGATGAACTGACTGGCTTGTGCTATGTTGGAAACCAAAATCTCGATGCCCTCAC CGGGTTCGTGGTGGCTCCCCTCTTTACTTATTTGGTCATTGGAACTTTGTTCATTGCTGC AGGTTTGGTGGCCTTGTTCAAAATTCGGTCAAATCTTCAAAAGGATGGGACAAAGACAGA CAAGTTAGAAAGACTGATGGTCAAGATTGGGGTGTTCTCAGTACTGTACACAGTTCCTGC AACGTGTGTGATTGCCTGTTATTTTTATGAAATCTCCAACTGGGCACTTTTTCGGTATTC TGCAGATGATTCCAACATGGCTGTTGAAATGTTGAAAATTTTTATGTCTTTGTTGGTGGG CATCACTTCAGGCATGTGGATTTGGTCTGCCAAAACTCTTCACACGTGGCAGAAGTGTTC CAACAGATTGGTGAATTCTGGAAAGGTAAAGAGAGAGAAGAGAGGAAATGGTTGGGTGAA GCCTGGAAAAGGCAGTGAGACTGTGGTATAAGGCTAGTCAGCCTCCATGCTTTCTTCATT TTGAAGGGGGGAATGCCAGCATTTTGGAGGAAATTCTACTAAAAGTTTTATGCAGTGAAT CTCAGTTTGAACAAACTAGCAACAATTAAGTGACCCCCGTCAACCCACTGCCTCCCACCC CGACCCCAGCATCAAAAAACCAATGATTTTGCTGCAGACTTTGGAATGATCCAAAATGGA AAAGCCAGTTAGAGGCTTTCAAAGCTGTGAAAAATCAAAACGTTGATCACTTTAGCAGGT TGCAGCTTGGAGCGTGGAGGTCCTGCCTAGATTCCAGGAAGTCCAGGGCGATACTGTTTT CCCCTGCAGGGTGGGATTTGAGCTGTGAGTTGGTAACTAGCAGGGAGAAATATTAACTTT TTTAACCCTTTACCATTTTAAATACTAACTGGGTCTTTCAGATAGCAAAGCAATCTATAA ACACTGGAAACGCTGGGTTCAGAAAAGTGTTACAAGAGTTTTATAGTTTGGCTGATGTAA CATAAACATCTTCTGTGGTGCGCTGTCTGCTGTTTAGAACTTTGTGGACTGCACTCCCAA GAAGTGGTGTTAGAATCTTTCAGTGCCTTTGTCATAAAACAGTTATTTGAACAAACAAAA GTACTGTACTCACACACATAAGGTATCCAGTGGATTTTTCTTCTCTGTCTTCCTCTCTTA AATTTCAACATCTCTCTTCTTGGCTGCTGCTGTTTTCTTCATTTTATGTTAATGACTCAA AAAAGGTATTTTTATAGAATTTTTGTACTGCAGCATGCTTAAAGAGGGGAAAAGGAAGGG TGATTCACTTTCTGACAATCACTTAATTCAGAGGAAAATGAGATTTACTAAGTTGACTTA CCTGACGGACCCCAGAGACCTATTGCATTGAGCAGTGGGGACTTAATATATTTTACTTGT GTGATTGCATCTATGCAGACGCCAGTCTGGAAGAGCTGAAATGTTAAGTTTCTTGGCAAC TTTGCATTCACACAGATTAGCTGTGTAATTTTTGTGTGTCAATTACAATTAAAAGCACAT TGTTGGACCATGACATAGTATACTCAACTGACTTTAAAACTATGGTCAACTTCAACTTGC ATTCTCAGAATGATAGTGCCTTTAAAAATTTTTTTATTTTTTAAAGCATAAGAATGTTAT CAGAATCTGGTCTACTTAGGACAATGGAGACTTTTTCAGTTTTATAAAGGGAACTGAGGA CAGCTAATCCAACTACTTGGTGCGTAATTGTTTCCTAGTAATTGGCAAAGGCTCCTTGTA AGATTTCACTGGAGGCAGTGTGGCCTGGAGTATTTATATGGTGCTTAATGAATCTCCAGA ATGCCAGCCAGAAGCCTGATTGGTTAGTAGGGAATAAAGTGTAGACCATATGAAATGAAC TGCAAACTCTAATAGCCCAGGTCTTAATTGCCTTTAGCAGAGGTATCCAAAGCTTTTAAA ATTTATGCATACGTTCTTCACAAGGGGGTACCCCCAGCAGCCTCTCGAAAATTGCACTTC TCTTAAAACTGTAACTGGCCTTTCTCTTACCTTGCCTTAGGCCTTCTAATCATGAGATCT TGGGGACAAATTGACTATGTCACAGGTTGCTCTCCTTGTAACTCATACCTGTCTGCTTCA GCAACTGCTTTGCAATGACATTTATTTATTAATTCATGCCTTAAAAAAATAGGAAGGGAA GCTTTTTTTTTTCTTTTTTTTTTTTTTCAATCACACTTTGTGGAAAAACATTTCCAGGGA CTCAAAATTCCAAAAAGGTGGTCAAATTCTGGAAGTAAGCATTTCCTCTTTTTTAAAAAT TTGGTTTGAGCCTTATGCCCATAGTTTGACATTTCCCTTTCTTCTTTCCTTTTTGTTTTT GTGTGGTTCTTGAGCTCTCTGACATCAAGATGCATGTAAAGTCGATTGTATGTTTTGGAA GGCAAAGTCTTGGCTTTTGAGACTGAAGTTAAGTGGGCACAGGTGGCCCCTGCTGCTGTG CCCAGTCTGAGTACCTTGGCTAGACTCTAGGTCAGGCTCCAGGAGCATGAGAATTGATCC CCAGAAGAACCATTTTAACTCCATCTGATACTCCATTGCCTATGAAATGTAAAATGTGAA CTCCCTGTGCTGCTTGTAGACAGTTCCCATAACTGTCCACGGCCCTGGAGCACGCACCCA GGGGCAGAGCCTGCCCTTACTCACGCTCTGCTCTGGTGTCTTGGGAGTTGTGCAGGGACT CTGGCCCAGGCAGGGGAAGGAAGACCAGGCGGTAGGGGACTGGTCTTGCTGTTAGAGTAT AGAGGTTTGTAATGCAGTTTTCTTCATAATGTGTCAGTGATTGTGTGACCAAGGCAGCAT CTAGCAGAAAGCCAGGCATGGAGTAGGTGATCGATACTTGTCAATGACTAAATAATAACA ATAAAAGAGCACTTGGGTGAATCTGGAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_012193 unedited
TTTCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA TTCGGCACGAGGCGGACGCGGCCGGCGCCGGCTTGTGGGCTCGCCGCCTGCAGCCATGAC CCTCGCAGCCTGTCCCTCGGCCTCGGCCCGGGACGTCTAAAATCCCACACAGTCGCGCGC AGCTGCTGGAGAGCCGGCCGCTGCCCCCTCGTCGCCGCATCACACTCCCGTCCCGGGAGC TGGGAGCAGCGCGGGCAGCCGGCGCCCCCGTGCAAACTGGGGGTGTCTGCCAGAGCAGCC CCAGCCGCTGCCGCTGCTACCCCCGATGCTGGCCATGGCCTGGCGGGGCGCAGGGCCGAG CGTCCCGGGGGCGCCCGGGGGCGTCGGTCTCAGTCTGGGGTTGCTCCTGCAGTTGCTGCT GCTCCTGGGGCCGGCGCGGGGCTTCGGGGACGAGGAAGAGCGGCGCTGCGACCCCATCCG CATCTCCATGTGCCAGAACCTCGGCTACAACGTGACCAAGATGCCCAACCTGGTTGGGCA CGAGCTGCAGACGGACGCCGAGCTGCAGCTGACAACTTTCACACCGCTCATCCAGTACGG CTGCTCCAGCCAGCTGCAGTTCTTCCTTTGTTCTGTTTATGTGCCAATGTGCACAGAGAA GATCAACATCCCCATTGGCCCATGCNGCNGCATGTGTCTTTCAGTCAAGAGACGCTGTGA ACCCGTCCCTGAAGAAATTGGNATTGCCTGGCCAGAGAGTCTGAACTGCAGCANATTCCC CACACAGAACGACCACNACCACATGTGCATGGAAGGGCCCAGTGATGAANNAGTGCCCNT TACTCACAAACCCCCATCCAGCCTGGGGGAAGAGTGTCACTCTGTGGGAACCAATTCTGA TCAC >OriGene 3' read for NM_012193 unedited
CTTTTTTTCCAATCCCCAATGCTCTTTTATTGTTATTATTTAGTCATTGACAAGTATCGA TCACCTACTCCATGCCTGGCTTTCTGCTAGATGCTGCCTTGGTCACACAATCACTGACAC ATTATGAAGAAAACTGCATTACAAACCTCTATACTCTAACAGCAAGACCATTCCCCTACC GCCTGGTCTTCCTTCCCCTGCCTGGGCCAGAGTCCCTGCACAACTCCCAAGACACCAGAG CAGAGCGTGAGTAAGGGCAGGCTCTGCCCCTGGGTGCGTGCTCCAGGGCCGTGGACAGTT ATGGGAACTGTCTACAAGCAGCACAGGGAGTTCACATTTTACATTTCATAGGCAATGGAG TATCAGATGGAGTTAAAATGGTTCTTCTGGGGATCAATTCTCATGCTCCTGGAGCCTGAC CTAGAGTCTAGCCAAGGTACTCAGACTGGGCACAGCAGCAGGGGCCACCTGTGCCCACTT AACTTCAGTCTCAAAAGCCAAGACTTTGCCTTCCAAAACATACAATCGACTTTACATGCA TCTTGATGTCAGAGAGCTCAAGAACCACACAAAAACAAAAAGGAAAGAAGAAAGGGAAAT GTCAAACTATGGGCATAAGGCTCAAACCAAATTTTTAAAAAAGAGGAAATGCTTACTTCC AGAATTTGACCACCTTTTTGGAATTTTGAGTCCCTGGAAATGTTTTTTCACAAAGTGTGA TTGAAAAAAAAAAAAAAGAAAAAAAAAAGCTCCCCTTCCTATTTTTTTAAAGCATGAATT AATAAATAAATGTCATTGGAAAGCAATTGCTGAAACAAACAGGGTTTGAGTTACAAGGGG AGCAACCTGTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012193 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012193.2, NP_036325.2 |
| RefSeq Size | 7391 bp |
| RefSeq ORF | 1614 bp |
| Locus ID | 8322 |
| UniProt ID | Q9ULV1 |
| Domains | FRI, Frizzled |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
| Gene Summary | This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families
,Voloshanenko, O;Gmach, P;Winter, J;Kranz, D;Boutros, M;,
FASEB J.
,PubMed ID 28733458
[FZD4]
|
|
FZD4 as a Mediator of ERG Oncogene–Induced WNT Signaling and Epithelial-to-Mesenchymal Transition in Human Prostate Cancer Cells
,Santosh Gupta, Kristiina Iljin, Henri Sara, John Patrick Mpindi, Tuomas Mirtti, Paula Vainio, Juha Rantala, Kalle Alanen, Matthias Nees, and Olli Kallioniemi,
Cancer Res., Sep 2010; 70: 6735 - 6745
[FZD4]
|
|
Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer
,Y Kawano, S Diez, P Uysal-Onganer, R S Darrington, J Waxman, R M Kypta,
British Journal of Cancer 100, 1165-1174 (10 March 2009)
[FZD4]
|
|
R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6
,ME Binnerts, KA Kim, JM Bright, SM Patel, K Tran, M Zhou, JM Leung, Y Liu, WE Lomas 3rd, M Dixon, SA Hazell, M Wagle, WS Nie, N Tomasevic, J Williams, X Zhan, MD Levy, WD Funk, and A Abo,
Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14700-5.
[FZD4]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217286 | FZD4 (Myc-DDK-tagged)-Human frizzled family receptor 4 (FZD4) |
CNY 4,000.00 |
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| RC217286L1 | Lenti ORF clone of Human frizzled family receptor 4 (FZD4), Myc-DDK-tagged |
CNY 6,400.00 |
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| RC217286L2 | Lenti ORF clone of Human frizzled family receptor 4 (FZD4), mGFP tagged |
CNY 6,400.00 |
|
| RC217286L3 | Lenti ORF clone of Human frizzled family receptor 4 (FZD4), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC217286L4 | Lenti ORF clone of Human frizzled family receptor 4 (FZD4), mGFP tagged |
CNY 6,400.00 |
|
| RG217286 | FZD4 (tGFP-tagged) - Human frizzled family receptor 4 (FZD4) |
CNY 5,600.00 |
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