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MID2 (NM_012216) Human Untagged Clone
CAT＃: SC115493
MID2 (untagged)-Human midline 2 (MID2), transcript variant 1
CNY 5,248.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FXY2; MRX101; RNF60; TRIM1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_012216, the custom clone sequence may differ by one or more nucleotides
ATGGGTGAAAGCCCAGCCTCCGTGGTTCTTAATGCCTCAGGAGGACTATTTTCACTAAAGATGGAAACAC TGGAGTCTGAATTGACCTGTCCAATCTGCCTAGAGTTGTTTGAAGACCCCCTTCTGCTCCCTTGTGCTCA CAGCCTCTGCTTCAGCTGTGCCCATCGCATTTTGGTATCAAGCTGCAGCTCTGGTGAATCCATTGAACCC ATTACTGCTTTCCAGTGTCCTACCTGCAGGTATGTTATCTCGCTGAACCACCGGGGCCTGGATGGCCTCA AGAGGAATGTGACTCTGCAGAACATTATTGATCGCTTCCAGAAGGCTTCAGTCAGTGGGCCCAATTCCCC TAGTGAGAGCCGCCGGGAAAGGACTTACAGGCCCACCACTGCCATGTCTAGCGAGCGAATTGCTTGCCAA TTCTGTGAGCAGGACCCGCCAAGGGATGCAGTAAAAACATGCATCACCTGTGAGGTCTCCTACTGTGACC GTTGCCTGCGGGCCACGCACCCCAACAAGAAACCTTTCACCAGCCACCGCCTGGTGGAACCAGTGCCAGA CACACATCTTCGAGGGATCACCTGCCTGGACCATGAGAATGAGAAAGTGAACATGTACTGTGTATCTGAT GACCAATTGATCTGTGCCTTATGCAAACTGGTGGGTCGTCACCGAGACCATCAGGTCGCATCCCTGAATG ATCGATTTGAGAAACTCAAGCAAACTCTGGAGATGAACCTCACCAACCTGGTTAAGCGCAACAGCGAACT AGAAAATCAAATGGCCAAACTAATACAGATCTGCCAGCAGGTTGAGGTGAATACTGCTATGCATGAGGCA AAACTTATGGAAGAATGTGACGAGTTGGTAGAGATCATCCAGCAGAGGAAGCAAATGATCGCTGTCAAAA TCAAAGAGACAAAGGTTATGAAACTGAGAAAGTTGGCACAGCAGGTTGCTAATTGCCGCCAGTGTCTTGA ACGGTCAACAGTCCTCATCAACCAAGCTGAGCATATCCTGAAAGAAAATGACCAGGCACGGTTTCTACAG TCTGCAAAAAATATTGCTGAGAGGGTCGCTATGGCAACTGCATCTTCTCAAGTTCTGATTCCAGACATCA ATTTTAATGATGCCTTTGAAAACTTTGCTTTAGATTTTTCCAGAGAAAAGAAACTGCTAGAGGGGTTAGA TTATTTAACAGCCCCAAACCCACCATCTATCCGAGAAGAACTCTGTACTGCCTCCCATGACACCATTACA GTCCACTGGATCTCGGATGATGAGTTCAGCATCAGCTCCTATGAGCTTCAGTACACCATATTCACTGGCC AGGCTAACTTCATCAGTAAGTCATGGTGTAGTTGGGGCCTGTGGCCAGAGATAAGGAAATGTAAGGAAGC AGTAAGCTGCTCAAGATTGGCCGGGGCGCCACGAGGCCTGTATAATTCAGTAGACAGCTGGATGATTGTT CCCAACATTAAACAGAACCATTACACAGTGCATGGACTCCAGAGCGGGACTCGCTACATCTTCATCGTTA AAGCCATAAACCAAGCCGGCAGCCGGAACAGTGAACCTACCCGACTAAAAACAAACAGCCAACCCTTTAA ATTGGATCCCAAAATGACTCACAAGAAGTTGAAGATCTCCAATGATGGATTGCAGATGGAGAAGGATGAA AGCTCTCTAAAGAAGAGCCACACCCCAGAGAGGTTTAGTGGCACAGGGTGCTATGGGGCAGCAGGAAATA TATTCATTGACAGTGGCTGCCACTATTGGGAGGTGGTCATGGGTTCCTCAACATGGTATGCAATTGGCAT TGCCTACAAATCAGCTCCAAAGAATGAATGGATTGGCAAGAATGCCTCCTCATGGGTCTTCTCTCGCTGC AATAGTAACTTCGTGGTGAGACACAACAACAAGGAAATGCTGGTGGATGTGCCCCCACACCTGAAGCGTC TGGGTGTCCTCCTGGATTATGACAACAATATGCTGTCTTTCTATGACCCAGCTAACTCTCTCCATCTTCA TACTTTTGATGTGACCTTCATTCTTCCAGTTTGTCCAACATTTACAATCTGGAACAAATCCCTAATGATC CTGTCTGGCTTGCCTGCCCCAGATTTTATTGATTACCCTGAGCGGCAGGAATGCAACTGCAGGCCTCAAG AATCCCCTTATGTTTCTGGGATGAAAACCTGTCATTAA >OriGene 5' read for NM_012216 unedited
CTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGTGTGGTGCTGCCGTGGTGTCATGGT GCTGCCCCTGGACTGAGGGGCGAAAACTCTTAAGTTTAGCTCGGGAGGCCCAGCTGCGGT AGCATCGCGGCCGCCGTGCTGTCCCCTGCGGGGCGCGCGGGCTGGCGGATCCCGGCTGCT GCGCGGCGTCGGCGACGGTAGCGGCAGCGGCGGCGAAGGCGGGCGGCGGCCTACAGTGGT AGCGGCGGCGGCGGCGACCGGGGCCCGGGAGCTCGCGCCGGAGCCCGAGCCAACCCGCTG CGGAGGCAGACGAGAGCCCAGCGCCCTCGAGCGAGCGGAGGAGATGGCTGGCACCTGNGA ACGCTATGGGTGAAAGCCCAGCCTCCGTGGTTCTTAATGCCTCAGGAGGACTATTTTCAC TAAAGATGGAAACACTGGAGTCTGAATTGACCTGTCCAATCTGCCTAGAGTTGTTTGAAG ACCCCCTTCTGCTCCCTTGTGCTCACAGCCTCTGCTTCAGCTGTGCCCATCGCATTNTGG TATCAAGCTGCAGCTCTGGTGAATCCATTGAACCCATTACTGCTTTCCAGTGTCCTACCT GCANGTATGTTATCTCGCTGAACCACCGGNGCCTGGATGGCCTCAGAGGAATGTGACTCT GCAGACATTATTGATCGCTTNCAGAAGGCTCAGTCAGTGGGCCCAATCCCCTAGTGAGAA GCGNCGGGAAAGGGACTACANGCCCACCACTGNCATGTCTAGCGAGCGAATNGCTTGCCA NTCTGTGAGCAGGACCCCGCAGGGATGCAGTAAAACATGCATCACTGTGNAGTCTCTACT GTGACCCGTGCTGCGGCCCACGCACCCCACAGAAACCTTNACCAGNCACCGNCTGGTGGA ACCAGTNGCAGACCCATCTTNNAGGGATCACTGNNCTGACATGAAATANAAAGGGACTGT ACTGGGATCTGAGACCAATGAACTGGCTTATCACCGGTGGGGCGNACCAAACCTGAGTCG ATCCGATGACGATNGAACTCAGCACTGGAATAAACTACACTGTTA >OriGene 3' read for NM_012216 unedited
GGCCGCAATCTANAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAGCCTCA AACAAACTTTATTTTGTGTCTCGTATATTGAACGCTAACTTAAGTGTAAGAGGGGAAAAA CTGCTGAACTAAAGAGCCAGTGAATTATATACTCTCCTGAAACTTAATGACAGGTTTTCA TCCCAAAAACATAAGGGGATTCTTGAGGCCTGCAGTTGCATTCCTGCCGCTCAAGGTAAT CAATAAAATCTGGGGCAGGCAAGCCAAACAGGATCATTAGGGATTTGTTCCAAATTGTAA ATGTTGGACAAACTGGAAAAATGAAGGGCACATCAAAAGTATGAAGATGGAGAGAGTTAG CTGGGTCATAAAAAGACAGCATATTGTTGTCATAATCCAGGAGGACACCCAGACGCTTCA GGTGTGGGGGCACATCCACCAACATTTCCTTGGTGTTGTGTCTCACCACCAAGTTACTAT TGCAGCGAGAGAAGACCCATGAGGAGGCATTCTTGCCAATCCATTCATTCTTTGGAGCTG ATTTGTAGGCAATGCCAATTGCATACCATGTTGAGGAACCCCTGACCACCCTCCAATAGT GGCAGCCACTGTCAATGAATATATTTCCTGCTGCCCCATAACACCCCTGTGCCACTAAAC CTCTCTGGGGGGTGGGCCCTTCTTTAGAAAGCTTTCATCCTTCTCCATTTGAAATCATCA TCGGAACCCTCAACCTCCTGGGACTATTTTTGGACCCAATTTAAACGCGCGGCCCGCCCT TCTTATCCCGCAACGGTTACTGTCCCGGNTTGCCGCCTGGGTTATGCGCTTAACAATAAA AACCAATCAGTTCCCCCTCTGAGATCCCGCCCCTGGACAGGGTTCTTTTCACAGCTGGGA CAACCTTCCCCTCGTTTCTCATATACACGCTCTTAAAATATTCCTGCCCCCCACCCGCTC CCTGAAATTTCACACCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012216 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012216.2, NP_036348.1 |
| RefSeq Size | 7345 bp |
| RefSeq ORF | 2148 bp |
| Locus ID | 11043 |
| UniProt ID | Q9UJV3 |
| Domains | zf-B_box, RING, BBC, SPRY, FN3 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229356 | MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 5,384.00 |
|
| RC229356L3 | Lenti-ORF clone of MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 7,700.00 |
|
| RC229356L4 | Lenti-ORF clone of MID2 (mGFP-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 7,700.00 |
|
| RG229356 | MID2 (tGFP-tagged) - Human midline 2 (MID2), transcript variant 1 |
CNY 6,370.00 |
|
| SC327991 | MID2 (untagged)-Human midline 2 (MID2) transcript variant 1 |
CNY 14,060.00 |
