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Hsc70 (HSPA8) (NM_006597) Human Untagged Clone
CAT＃: SC115973
HSPA8 (untagged)-Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1
CNY 4,816.00
| Cited in 4 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115973 sequence for NM_006597 edited (data generated by NextGen Sequencing)
ATGTCCAAGGGACCTGCAGTTGGTATTGATCTTGGCACCACCTACTCTTGTGTGGGTGTT TTCCAGCACGGAAAAGTCGAGATAATTGCCAATGATCAGGGAAACCGAACCACTCCAAGC TATGTCGCCTTTACGGACACTGAACGGTTGATCGGTGATGCCGCAAAGAATCAAGTTGCA ATGAACCCCACCAACACAGTTTTTGATGCCAAACGTCTGATTGGACGCAGATTTGATGAT GCTGTTGTCCAGTCTGATATGAAACATTGGCCCTTTATGGTGGTGAATGATGCTGGCAGG CCCAAGGTCCAAGTAGAATACAAGGGAGAGACCAAAAGCTTCTATCCAGAGGAGGTGTCT TCTATGGTTCTGACAAAGATGAAGGAAATTGCAGAAGCCTACCTTGGGAAGACTGTTACC AATGCTGTGGTCACAGTGCCAGCTTACTTTAATGACTCTCAGCGTCAGGCTACCAAAGAT GCTGGAACTATTGCTGGTCTCAATGTACTTAGAATTATTAATGAGCCAACTGCTGCTGCT ATTGCTTACGGCTTAGACAAAAAGGTTGGAGCAGAAAGAAACGTGCTCATCTTTGACCTG GGAGGTGGCACTTTTGATGTGTCAATCCTCACTATTGAGGATGGAATCTTTGAGGTCAAG TCTACAGCTGGAGACACCCACTTGGGTGGAGAAGATTTTGACAACCGAATGGTCAACCAT TTTATTGCTGAGTTTAAGCGCAAGCATAAGAAGGACATCAGTGAGAACAAGAGAGCTGTA AGACGCCTCCGTACTGCTTGTGAACGTGCTAAGCGTACCCTCTCTTCCAGCACCCAGGCC AGTATTGAGATCGATTCTCTCTATGAAGGAATCGACTTCTATACCTCCATTACCCGTGCC CGATTTGAAGAACTGAATGCTGACCTGTTCCGTGGCACCCTGGACCCAGTAGAGAAAGCC CTTCGAGATGCCAAACTAGACAAGTCACAGATTCATGATATTGTCCTGGTTGGTGGTTCT ACTCGTATCCCCAAGATTCAGAAGCTTCTCCAAGACTTCTTCAATGGAAAAGAACTGAAT AAGAGCATCAACCCTGATGAAGCTGTTGCTTATGGTGCAGCTGTCCAGGCAGCCATCTTG TCTGGAGACAAGTCTGAGAATGTTCAAGATTTGCTGCTCTTGGATGTCACTCCTCTTTCC CTTGGTATTGAAACTGCTGGTGGAGTCATGACTGTCCTCATCAAGCGTAATACCACCATT CCTACCAAGCAGACACAGACCTTCACTACCTATTCTGACAACCAGCCTGGTGTGCTTATT CAGGTTTATGAAGGCGAGCGTGCCATGACAAAGGATAACAACCTGCTTGGCAAGTTTGAA CTCACAGGCATACCTCCTGCACCCCGAGGTGTTCCTCAGATTGAAGTCACTTTTGACATT GATGCCAATGGTATACTCAATGTCTCTGCTGTGGACAAGAGTACGGGAAAAGAGAACAAG ATTACTATCACTAATGACAAGGGCCGTTTGAGCAAGGAAGACATTGAACGTATGGTCCAG GAAGCTGAGAAGTACAAAGCTGAAGATGAGAAGCAGAGGGACAAGGTGTCATCCAAGAAT TCACTTGAGTCCTATGCCTTCAACATGAAAGCAACTGTTGAAGATGAGAAACTTCAAGGC AAGATTAACGATGAGGACAAACAGAAGATTCTGGACAAGTGTAATGAAATTATCAACTGG CTTGATAAGAATCAGACTGCCGAGAAGGAAGAATTTGAACATCAACAGAAAGAGCTGGAG AAAGTTTGCAACCCCATCATCACCAAGCTGTACCAGAGTGCAGGAGGCATGCCAGGAGGA ATGCCTGGGGGATTTCCTGGTGGTGGAGCTCCTCCCTCTGGTGGTGCTTCCTCAGGGCCC ACCATTGAAGAGGTTGATTAA Clone variation with respect to NM_006597.4 1761 t=>c >OriGene 5' read for NM_006597 unedited
GGTTACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCTCGCCTGCA GCTCTTGGGTTTTTTGTGGCTTCCTTCGTTATTGGAGCCAGGCCTACATCCCAGCAACCA TGTCCAAGGGACCTGCAGTTGGTATTGATCTTGGCACCACCTACTCTTGTGTGGGTGTTT TCCAGCACGGTAAAAGTCGAGATAATTGCCAATGATCAGGGAAACCGAACCACTCCAAGC TATGTCGCCTTTACGGACACTGAACGGTTGATCGGTGATGCCGCAAAGAATCAAGTTGCA ATGAACCCCACCAACACAGTTTTTGATGCCAAACGTCTGATTGGACGCAGATTTGATGAT GCTGTTGTCCAGTCTGATATGAAACATTGGCCCTTTATGGTGGTGAATGATGCTGGCAGG CCCAAGGTCCAAGTAGAATACAAGGGAGAGACCAAAAGCTTCTATCCAGAGGAGGTGTCT TCTATGGTTCTGACAAAGATGAAGGAAATTGCAGAAGCCTACCTTGGGAAGACTGTTACC AATGCTGTGGTCACAGTGCCAGCTTACTTTAATGACTCTCAGCGTCAGGCTACCAAAGAT GCTGGAACTATTGCTGGTCTCAATGTACTTAGAATTATTAATGAGCCAACTGCTGCTGCT ATTGCTTACGGCTTAGACAAAAAGGTTGGAGCAGAAAGAAACGTGCTCATCTTTGACCTG GGAGGTGGCACTTTTGATGTGTCAATCCTCACTATTNGAGATGGAATCTTTGAGGTCAAG TCTACAGCTGGAGACACCCACTTGGGTGGAGAAGATTNTGACAACCGAATGGGTCACCAT TTTATTGCTGAGTTTAAGCGCAAGCTTAGAAGGACATCAGTGAGAACAAGAGAGCTGTNA GAACGCTCCGTACTGCCTGC >OriGene 3' read for NM_006597 unedited
ATGGCAACTTCCAGGGCCAGNATAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGGAT CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT TTTTGCCAATTTTAAATAGTTTTATTTAAGACATTGCATTTTCCACTTACAATACAGTGT TTATAAAGTGCAATGTTATTTCCTTCCCCTGTGCATATGTTCCATATTCAAGTATTGAGA ATGCCCAGTAACTTACTATAGCAGCTTAACTTTTTAAAACTGCCACAGAATTTGCTACGA ATTTAGGTCCTTCAAATGTTTTAAATGTGTGGAACAATGCTACATCTACACTTGGTTGGC TTAATCAACCTCTTCAATGGTGGGCCCTGAGGAAGCACCACCAGAGGGAGGAGCTCCACC ACCAGGAAATCCCCCAGGCATTCCTCCTGGCATGCCTCCTGCACTCTGGTACAGCTTGGT GATGATGGGGTTGCAAACTTTCTCCAGCTCTTTCTGTTGATGTTCAAATTCTTCCTTCTC GGCAGTCTGATTCTTATCAAGCCAGTTGATAATTTCATTACACTTGTCCAGAATCTTCTG TTTGTCCTCATCGTTAATCTTGCCTTGAAGTTTCTCATCTTCAACAGTTGCTTTCATGTT GAAGGCATAGGACTCAAGTGAATTCTTGGATGACANCCTTGTCCCTCTGCTTCTCATCTT CAGCTTTGTACTTCTCAGCTTCCTGGACCATACGTTCAATGGTCTTCTTGCTCAAACGNC CCTTGNATTAGNGATAGTAATCTTGGTCTCTTTTCCCCGTACTCTTNGCCACAGCAGAGA CATTGAGGATACCATTGGCATCATGGCAAAAGTGACTTCATCCTGAGACACC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006597 |
| Insert Size | 2190 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006597.3, NP_006588.1 |
| RefSeq Size | 2276 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 3312 |
| UniProt ID | P11142 |
| Domains | HSP70 |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome |
| Gene Summary | This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Hsc70 facilitates TGF-ß-induced activation of Smad2/3 in fibroblastic NRK-49F cells
,Ikezaki, M;Higashimoto, N;Matsumura, K;Ihara, Y;,
Biochem. Biophys. Res. Commun.
,PubMed ID 27320859
[HSPA8]
|
|
Isoform Selective Genetic Inhibition of Constitutive Cytosolic Hsp70 Activity Promotes Client Tau Degradation Using an Altered Co-chaperone Complement
,Stothert, UK;Sabbagh, JJ;Chang, L;Stevens, SM;Jr, E;,
JOURNAL OF BIOLOGICAL CHEMISTRY
,PubMed ID 25864199
[HSPA8]
|
|
The active Hsc70/tau complex can be exploited to enhance tau turnover without damaging microtubule dynamics
,Fontaine, SN;Martin, MD;Akoury, E;Assimon, VA;Borysov, S;Nordhues, BA;Sabbagh, JJ;Cockman, M;Gestwicki, JE;Zweckstetter, M;Dickey, CA;,
Hum. Mol. Genet.
,PubMed ID 25882706
[HSPA8]
|
|
C-Mannosylated peptides derived from the thrombospondin type 1 repeat interact with Hsc70 to modulate its signaling in RAW264.7 cells
,Yoshito Ihara, Shino Manabe, Midori Ikezaki, Yoko Inai, In-Sook Lee Matsui, Yuriko Ohta, Eiji Muroi, and Yukishige Ito,
Glycobiology, Oct 2010; 20: 1298 - 1310
[HSPA8]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202209 | HSPA8 (Myc-DDK-tagged)-Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1 |
CNY 4,808.00 |
|
| RC202209L1 | Lenti ORF clone of Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1, Myc-DDK-tagged |
CNY 7,208.00 |
|
| RC202209L2 | Lenti ORF clone of Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC202209L3 | Lenti ORF clone of Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1, Myc-DDK-tagged |
CNY 7,208.00 |
|
| RC202209L4 | Lenti ORF clone of Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1, mGFP tagged |
CNY 7,208.00 |
|
| RG202209 | HSPA8 (tGFP-tagged) - Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1 |
CNY 6,408.00 |
|
| SC322471 | HSPA8 (untagged)-Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1 |
CNY 4,816.00 |
