TAF10 (NM_006284) Human Untagged Clone

CAT#: SC116191

TAF10 (untagged)-Human TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa (TAF10)



  "NM_006284" in other vectors (4)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-TAF10 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms TAF2A; TAF2H; TAFII30
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006284, the custom clone sequence may differ by one or more nucleotides


ATGAGCTGCAGCGGCTCCGGCGCGGACCCCGAGGCGGCGCCGGCCTCCGCCGCCTCGGCCCCGGGCCCCG
CGCCCCCGGTCTCGGCTCCCGCCGCGCTGCCCTCCAGCACCGCCGCGGAGAACAAGGCCAGCCCCGCGGG
GACAGCGGGGGGACCTGGGGCTGGAGCAGCTGCTGGGGGCACGGGACCCTTGGCGGCGCGGGCCGGGGAG
CCAGCTGAGCGGCGTGGGGCGGCTCCGGTGTCGGCGGGTGGCGCGGCGCCCCCGGAGGGGGCCATATCTA
ACGGGGTTTACGTACTGCCGAGCGCGGCCAACGGAGACGTGAAGCCCGTGGTGTCCAGCACGCCTTTGGT
GGACTTCTTGATGCAGCTGGAAGATTACACGCCTACGATCCCAGATGCAGTGACTGGTTACTACCTGAAC
CGTGCTGGCTTTGAGGCCTCAGACCCACGCATAATTCGGCTCATCTCCTTAGCTGCCCAGAAATTCATCT
CAGATATTGCCAATGATGCCCTACAGCACTGCAAAATGAAGGGCACGGCCTCCGGCAGCTCCCGGAGCAA
GAGCAAGGACCGCAAGTACACTCTAACCATGGAGGACTTGACCCCTGCCCTCAGCGAGTATGGCATCAAT
GTGAAGAAGCCGCACTACTTCACCTGA


>OriGene 5' read for NM_006284 unedited
TTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCCGGCCCGATGAGC
TGCAGCGGCTCCGGCGCGGACCCCGAGGCGGCGCCGGCCTCCGCCGCCTCGGCCCCGGGC
CCCGCGCCCCCGGTCTCGGCTCCCGCCGCGCTGCCCTCCAGCACCGCCGCGGAGAACAAG
GCCAGCCCCGCGGGGACAGCGGGGGGACCTGGGGCTGGAGCAGCTGCTGGGGGCACGGGA
CCCTTGGCGGCGCGGGCCGGGGAGCCAGCTGAGCGGCGTGGGGCGGCTCCGGTGTCGGCG
GGTGGCGCGGCGCCCCCGGAGGGGGCCATATCTAACGGGGTTTACGTACTGCCGAGCGCG
GCCAACGGAGACGTGAAGCCCGTGGTGTCCAGCACGCCTTTGGTGGACTTCTTGATGCAG
CTGGAAGATTACACGCCTACGATCCCAGATGCAGTGACTGGTTACTACCTGAACCGTGCT
GGCTTTGAGGCCTCAGACCCACGCATAATTCGGCTCATCTCCTTAGCTGCCCAGAAATTC
ATCTCAGATATTGCCAATGATGCCCTACAGCACTGCAAAATGAAGGGCACAGCCTCCGGC
AGCTCCCGGAGCAAAGAGCAGGACCGCAAGTACACTCTAACCATGGAGGACTTGACCCCT
GCCCTCAGCGAGTATGGCATCAATGTGAAGAAGCCGCACTACTTCACCTGAGCCACCCAA
CCTAAATGTACTTATCTGTCCCCATGTCCCCACACCAGCCTGTTTTCATAAATAAACGTT
ATGTGACAGGCCNNNNNNNNNNNNNTNNNNNTNNNNNNGNCCCNNNACTCCAACCCCTAT
TNCCCTAAACAAAATATTTCTCTTGCCACTTTAAAAATCAAAAAACCCCCCCCTCTCTAA
TTGCGGCCNGGGATAAGTGTTTCTGAAAAAACCGGGGGCACCT
>OriGene 3' read for NM_006284 unedited
GGGGGGNNNNGGGGGGNNNNNNNNNGTNNNANNTTNTNNNNNNNNGGGTTTTTCTGTGCC
GATAGAAGATTTCTTNATGTTAAAACAGNNCTGGTGTGGGGNACATGGGGNACAGCAAAG
TACATTTAGGTTGGGTGGCTCAGGTGAAGTAGTGCGGCTTCTTCACATTGATGCCATACT
CGCTGAGGGCAGGGGTCAAGTCCTCCATGGTTAGAGTGTACTTGCGGTCCTTGCTCTTGC
TCCGGGAGCTGCCGGAGGCTGTGCCCTTCATTTTGCAGTGCTGTAGGGCATCATTGGCAA
TATCTGAGATGAATTTCTGGGCAGCTAAGGAGATGAGCCGAATTATGCGTGGGTCTGAGG
CCTCAAAGCCAGCACGGTTCAGGTAGTAACCAGTCACTGCATCTGGGATCGTAGGCGTGT
AATCTTCCAGCTGCATCAAGAAGTCCACCAAAGGCGTGCTGGACACCACGGGCTTCACGT
CTCCGTTGGCCGCGCTCGGCAGTACGTAAACCCCGTTAGATATGGCCCCCTCCGGGGGCG
CCGCGCCACCCGCCGACACCGGAGCCGTCCCACGCCGCTCAGCTGGCTCCCCGGCCCGCG
CCGCCAAGGGTCCCGTGCCCCCAGCAGCTGCTCCAGCCCCAGGTCCCCCCGCTGTCCCCG
CGGGGCTGGCCTTGTTCTCCGCGGCGGTGCTGGAGGGCACCGCGGCGGCAGCCGATACCG
GGGGCGCGGGGCCCCGGGGCCGAGGCGGCGGATGCCGTCGCCGCCTTGGGGTCCCGCGCC
GTACCGTTGTAGCTTATCGGGCCCGGCCTTGTGTCCGAATTCCTGGCCGCCCTATAGTGA
GTCGTATTACAAAATTCTGCACGGTTCACTAAACGAACCTTTGCTTATATAACACCTCCA
CCCGTTCACGCCTTACCCCCCTTTTGGCGTAAACGGGGCGGGG
Restriction Sites NotI-NotI     
ACCN NM_006284
Insert Size 3760 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006284.2, NP_006275.1
RefSeq Size 762 bp
RefSeq ORF 657 bp
Locus ID 6881
UniProt ID Q12962
Domains TFIID_30kD
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Basal transcription factors
Gene Summary Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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