TAF10 (NM_006284) Human Untagged Clone
CAT#: SC116191
TAF10 (untagged)-Human TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa (TAF10)
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | TAF2A; TAF2H; TAFII30 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_006284, the custom clone sequence may differ by one or more nucleotides
ATGAGCTGCAGCGGCTCCGGCGCGGACCCCGAGGCGGCGCCGGCCTCCGCCGCCTCGGCCCCGGGCCCCG CGCCCCCGGTCTCGGCTCCCGCCGCGCTGCCCTCCAGCACCGCCGCGGAGAACAAGGCCAGCCCCGCGGG GACAGCGGGGGGACCTGGGGCTGGAGCAGCTGCTGGGGGCACGGGACCCTTGGCGGCGCGGGCCGGGGAG CCAGCTGAGCGGCGTGGGGCGGCTCCGGTGTCGGCGGGTGGCGCGGCGCCCCCGGAGGGGGCCATATCTA ACGGGGTTTACGTACTGCCGAGCGCGGCCAACGGAGACGTGAAGCCCGTGGTGTCCAGCACGCCTTTGGT GGACTTCTTGATGCAGCTGGAAGATTACACGCCTACGATCCCAGATGCAGTGACTGGTTACTACCTGAAC CGTGCTGGCTTTGAGGCCTCAGACCCACGCATAATTCGGCTCATCTCCTTAGCTGCCCAGAAATTCATCT CAGATATTGCCAATGATGCCCTACAGCACTGCAAAATGAAGGGCACGGCCTCCGGCAGCTCCCGGAGCAA GAGCAAGGACCGCAAGTACACTCTAACCATGGAGGACTTGACCCCTGCCCTCAGCGAGTATGGCATCAAT GTGAAGAAGCCGCACTACTTCACCTGA >OriGene 5' read for NM_006284 unedited
TTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCCGGCCCGATGAGC TGCAGCGGCTCCGGCGCGGACCCCGAGGCGGCGCCGGCCTCCGCCGCCTCGGCCCCGGGC CCCGCGCCCCCGGTCTCGGCTCCCGCCGCGCTGCCCTCCAGCACCGCCGCGGAGAACAAG GCCAGCCCCGCGGGGACAGCGGGGGGACCTGGGGCTGGAGCAGCTGCTGGGGGCACGGGA CCCTTGGCGGCGCGGGCCGGGGAGCCAGCTGAGCGGCGTGGGGCGGCTCCGGTGTCGGCG GGTGGCGCGGCGCCCCCGGAGGGGGCCATATCTAACGGGGTTTACGTACTGCCGAGCGCG GCCAACGGAGACGTGAAGCCCGTGGTGTCCAGCACGCCTTTGGTGGACTTCTTGATGCAG CTGGAAGATTACACGCCTACGATCCCAGATGCAGTGACTGGTTACTACCTGAACCGTGCT GGCTTTGAGGCCTCAGACCCACGCATAATTCGGCTCATCTCCTTAGCTGCCCAGAAATTC ATCTCAGATATTGCCAATGATGCCCTACAGCACTGCAAAATGAAGGGCACAGCCTCCGGC AGCTCCCGGAGCAAAGAGCAGGACCGCAAGTACACTCTAACCATGGAGGACTTGACCCCT GCCCTCAGCGAGTATGGCATCAATGTGAAGAAGCCGCACTACTTCACCTGAGCCACCCAA CCTAAATGTACTTATCTGTCCCCATGTCCCCACACCAGCCTGTTTTCATAAATAAACGTT ATGTGACAGGCCNNNNNNNNNNNNNTNNNNNTNNNNNNGNCCCNNNACTCCAACCCCTAT TNCCCTAAACAAAATATTTCTCTTGCCACTTTAAAAATCAAAAAACCCCCCCCTCTCTAA TTGCGGCCNGGGATAAGTGTTTCTGAAAAAACCGGGGGCACCT >OriGene 3' read for NM_006284 unedited
GGGGGGNNNNGGGGGGNNNNNNNNNGTNNNANNTTNTNNNNNNNNGGGTTTTTCTGTGCC GATAGAAGATTTCTTNATGTTAAAACAGNNCTGGTGTGGGGNACATGGGGNACAGCAAAG TACATTTAGGTTGGGTGGCTCAGGTGAAGTAGTGCGGCTTCTTCACATTGATGCCATACT CGCTGAGGGCAGGGGTCAAGTCCTCCATGGTTAGAGTGTACTTGCGGTCCTTGCTCTTGC TCCGGGAGCTGCCGGAGGCTGTGCCCTTCATTTTGCAGTGCTGTAGGGCATCATTGGCAA TATCTGAGATGAATTTCTGGGCAGCTAAGGAGATGAGCCGAATTATGCGTGGGTCTGAGG CCTCAAAGCCAGCACGGTTCAGGTAGTAACCAGTCACTGCATCTGGGATCGTAGGCGTGT AATCTTCCAGCTGCATCAAGAAGTCCACCAAAGGCGTGCTGGACACCACGGGCTTCACGT CTCCGTTGGCCGCGCTCGGCAGTACGTAAACCCCGTTAGATATGGCCCCCTCCGGGGGCG CCGCGCCACCCGCCGACACCGGAGCCGTCCCACGCCGCTCAGCTGGCTCCCCGGCCCGCG CCGCCAAGGGTCCCGTGCCCCCAGCAGCTGCTCCAGCCCCAGGTCCCCCCGCTGTCCCCG CGGGGCTGGCCTTGTTCTCCGCGGCGGTGCTGGAGGGCACCGCGGCGGCAGCCGATACCG GGGGCGCGGGGCCCCGGGGCCGAGGCGGCGGATGCCGTCGCCGCCTTGGGGTCCCGCGCC GTACCGTTGTAGCTTATCGGGCCCGGCCTTGTGTCCGAATTCCTGGCCGCCCTATAGTGA GTCGTATTACAAAATTCTGCACGGTTCACTAAACGAACCTTTGCTTATATAACACCTCCA CCCGTTCACGCCTTACCCCCCTTTTGGCGTAAACGGGGCGGGG |
Restriction Sites | NotI-NotI |
ACCN | NM_006284 |
Insert Size | 3760 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006284.2, NP_006275.1 |
RefSeq Size | 762 bp |
RefSeq ORF | 657 bp |
Locus ID | 6881 |
UniProt ID | Q12962 |
Domains | TFIID_30kD |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Basal transcription factors |
Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204138 | TAF10 (Myc-DDK-tagged)-Human TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa (TAF10) |
CNY 2,400.00 |
|
RC204138L3 | Lenti ORF clone of Human TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa (TAF10), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204138L4 | Lenti ORF clone of Human TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa (TAF10), mGFP tagged |
CNY 5,890.00 |
|
RG204138 | TAF10 (tGFP-tagged) - Human TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa (TAF10) |
CNY 4,370.00 |