XRCC1 (NM_006297) Human Untagged Clone
CAT#: SC116204
XRCC1 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 1 (XRCC1)
CNY 7,080.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | RCC; SCAR26 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>NCBI ORF sequence for NM_006297, the custom clone sequence may differ by one or more nucleotides
ATGCCGGAGATCCGCCTCCGCCATGTCGTGTCCTGCAGCAGCCAGGACTCGACTCACTGTGCAGAAAATC TTCTCAAGGCAGACACTTACCGAAAATGGCGGGCAGCCAAGGCAGGCGAGAAGACCATCTCTGTGGTCCT ACAGTTGGAGAAGGAGGAGCAGATACACAGTGTGGACATTGGGAATGATGGCTCAGCTTTCGTGGAGGTG CTGGTGGGCAGTTCAGCTGGAGGCGCTGGGGAGCAAGACTATGAGGTCCTTCTGGTCACCTCATCTTTCA TGTCCCCTTCCGAGAGCCGCAGTGGCTCAAACCCCAACCGCGTTCGCATGTTTGGGCCTGACAAGCTGGT CCGGGCAGCCGCCGAGAAGCGCTGGGACCGGGTCAAAATTGTTTGCAGCCAGCCCTACAGCAAGGACTCC CCCTTTGGCTTGAGTTTTGTACGGTTTCATAGCCCCCCAGACAAAGATGAGGCAGAGGCCCCGTCCCAGA AGGTGACAGTGACCAAGCTTGGCCAGTTCCGTGTGAAGGAGGAGGATGAGAGCGCCAACTCTCTGAGGCC GGGGGCTCTCTTCTTCAGCCGGATCAACAAGACATCCCCAGTCACAGCCAGCGACCCAGCAGGACCTAGC TATGCAGCTGCTACCCTCCAGGCTTCTAGTGCTGCCTCCTCAGCCTCTCCAGTCTCCAGGGCCATAGGCA GCACCTCCAAGCCCCAGGAGTCTCCCAAAGGGAAGAGGAAGTTGGATTTGAACCAAGAAGAAAAGAAGAC CCCCAGCAAACCACCAGCCCAGCTGTCGCCATCTGTTCCCAAGAGACCTAAATTGCCAGCTCCAACTCGT ACCCCAGCCACAGCCCCAGTCCCTGCCCGAGCACAGGGGGCAGTGACAGGCAAACCCCGAGGAGAAGGCA CCGAGCCCAGACGACCCCGAGCTGGCCCAGAGGAGCTGGGGAAGATCCTTCAGGGTGTGGTAGTGGTGCT GAGTGGCTTCCAGAACCCCTTCCGCTCCGAGCTGCGAGATAAGGCCCTAGAGCTTGGGGCCAAGTATCGG CCAGACTGGACCCGGGACAGCACGCACCTCATCTGTGCCTTTGCCAACACCCCCAAGTACAGCCAGGTCC TAGGCCTGGGAGGCCGCATCGTGCGTAAGGAGTGGGTGCTGGACTGTCACCGCATGCGTCGGCGGCTGCC CTCCCAGAGGTACCTCATGGCAGGGCCAGGTTCCAGCAGTGAGGAGGATGAGGCCTCTCACAGCGGTGGC AGCGGAGATGAAGCCCCCAAGCTTCCTCAGAAGCAACCCCAGACCAAAACCAAGCCCACTCAGGCAGCTG GACCCAGCTCACCCCAGAAGCCCCCAACCCCTGAAGAGACCAAAGCAGCCTCACCAGTGCTCCAGGAAGA TATAGACATTGAGGGGGTACAGTCAGAAGGACAGGACAATGGGGCGGAAGATTCTGGGGACACAGAGGAT GAGCTGAGGAGGGTGGCAGAGCAGAAGGAACACAGACTGCCCCCTGGCCAGGAGGAGAATGGGGAAGACC CGTATGCAGGCTCCACGGATGAGAACACGGACAGTGAGGAACACCAGGAGCCTCCTGATCTGCCAGTCCC TGAGCTCCCAGATTTCTTCCAGGGCAAGCACTTCTTTCTTTACGGGGAGTTCCCTGGGGACGAGCGGCGG AAACTCATCCGATACGTCACAGCCTTCAATGGGGAGCTCGAGGACTATATGAGTGACCGGGTTCAGTTTG TGATCACAGCACAGGAATGGGATCCCAGCTTTGAGGAGGCCCTGATGGACAACCCCTCCCTGGCATTCGT TCGTCCCCGATGGATCTACAGTTGCAATGAGAAGCAGAAGTTACTTCCTCACCAGCTCTATGGGGTGGTG CCGCAAGCCTGA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | NotI-NotI |
ACCN | NM_006297 |
Insert Size | 2550 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006297.1, NP_006288.1 |
RefSeq Size | 2083 bp |
RefSeq ORF | 1902 bp |
Locus ID | 7515 |
UniProt ID | P18887 |
Domains | BRCT, XRCC1_N |
Protein Families | Druggable Genome |
Protein Pathways | Base excision repair |
Gene Summary | The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
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SDS |
Resources
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RC204952L4 | Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 1 (XRCC1), mGFP tagged |
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