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RAD21 (NM_006265) Human Untagged Clone
CAT＃: SC116225
RAD21 (untagged)-Human RAD21 homolog (S. pombe) (RAD21)
CNY 7,056.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDLS4; hHR21; HR21; HRAD21; MCD1; MGS; NXP1; SCC1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116225 sequence for NM_006265 edited (data generated by NextGen Sequencing)
ATGTTCTACGCACATTTTGTTCTCAGTAAAAGAGGGCCTCTGGCCAAAATTTGGCTAGCG GCCCATTGGGATAAGAAGCTAACCAAAGCCCATGTGTTCGAGTGTAATTTAGAGAGCAGC GTGGAGAGTATCATCTCACCAAAGGTGAAAATGGCATTACGGACATCAGGACATCTCTTA CTGGGAGTAGTTCGAATCTATCACAGGAAAGCCAAATACCTTCTTGCAGACTGTAATGAA GCATTCATTAAGATAAAGATGGCTTTTCGGCCAGGTGTGGTTGACCTGCCTGAGGAAAAT CGGGAAGCAGCTTATAATGCCATTACTTTACCTGAAGAATTTCATGACTTTGATCAGCCA CTGCCTGACTTAGATGACATCGATGTGGCCCAGCAGTTCAGCTTGAATCAGAGTAGAGTG GAAGAGATAACCATGAGAGAAGAAGTTGGGAACATCAGTATTTTACAAGAAAATGATTTT GGTGATTTTGGAATGGATGATCGTGAGATAATGAGAGAAGGCAGTGCTTTTGAGGATGAC GACATGTTAGTAAGCACTACTACTTCTAACCTCCTATTAGAGTCTGAACAGAGCACCAGC AATCTGAATGAGAAAATTAACCATTTAGAATATGAAGATCAATATAAGGATGATAATTTT GGAGAAGGAAATGATGGTGGAATATTAGATGACAAACTTATTAGTAATAATGATGGCGGT ATCTTTGATGATCCCCCTGCCCTCTCTGAGGCAGGGGTGATGTTGCCAGAGCAGCCTGCA CATGACGATATGGATGAGGATGATAATGTATCAATGGGTGGGCCTGATAGTCCTGATTCA GTGGATCCCGTTGAACCAATGCCAACCATGACTGATCAAACAACACTTGTTCCAAATGAG GAAGAAGCATTTGCATTGGAGCCTATTGATATAACTGTTAAAGAAACAAAAGCCAAGAGG AAGAGGAAGCTAATTGTTGACAGTGTCAAAGAGTTGGATAGCAAGACAATTAGAGCCCAA CTTAGTGATTATTCAGATATTGTTACTACTTTGGATCTGGCACCGCCCACCAAGAAATTG ATGATGTGGAAAGAGACAGGAGGAGTAGAAAAACTGTTTTCTTTACCTGCTCAGCCTTTG TGGAATAACAGACTACTGAAGCTCTTTACACGCTGTCTTACACCGCTTGTACCAGAAGAC CTTAGAAAAAGGAGGAAAGGAGGAGAGGCAGATAATTTGGATGAATTCCTCAAAGAATTT GAAAATCCAGAGGTTCCTAGAGAGGACCAGCAACAGCAGCATCAGCAGCGTGATGTTATC GATGAGCCCATTATTGAAGAGCCAAGCCGCCTCCAGGAGTCAGTGATGGAGGCCAGCAGA ACAAACATAGATGAGTCAGCTATGCCTCCACCACCACCTCAGGGAGTTAAGCGAAAAGCT GGACAAATTGACCCAGAGCCTGTGATGCCTCCTCAGCAGGTAGAGCAGATGGAAATACCA CCTGTAGAGCTTCCCCCAGAAGAACCTCCAAATATCTGTCAGCTAATACCAGAGTTAGAA CTTCTGCCAGAAAAAGAGAAGGAGAAAGAGAAGGAAAAAGAAGATGATGAAGAGGAAGAG GATGAAGATGCATCAGGGGGCGATCAAGATCAGGAAGAAAGAAGATGGAACAAAAGGACT CAGCAGATGCTTCATGGTCTTCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGT TTGCTTGAGTTATGTCGAAATACGAACAGAAAACAAGCTGCCGCAAAGTTCTACAGCTTC TTGGTTCTTAAAAAGCAGCAAGCTATTGAGCTGACACAGGAAGAACCGTACAGTGACATC ATCGCAACACCTGGACCAAGGTTCCATATTATATAA Clone variation with respect to NM_006265.2 >OriGene 5' read for NM_006265 unedited
GTAACACGACTCTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGATTTGAACCGAG GATTTGGGCGGCAGGAAGAGCCGCGGCGTAACGGCAGCCATCTTGTTTGTTTGAGTGAAT CGGAAAGGAGGCGCCGGCTGTGGCGGCGGCGGGAGCTGCTCGGAAGCTACACCTCGCAAG GGCTCCCCCCTTTCCCCACCCCCTCCCCCGACCCTTTTCCCCTCCCCGGGCCACCCAGCC CGCCCAACTCCCAGCGGAGAGCAAGGTTTTCTTCTGTTTTCATAGCCAGCCAGAACAATG TTCTACGCACATTTTGTTCTCAGTAAAAGAGGGCCTCTGGCCAAAATTTGGCTAGCGGCC CATTGGGATAAGAAGCTAACCAAAGCCCATGTGTTCGAGTGTAATTTAGAGAGCAGCGTG GAGAGTATCATCTCACCAAAGGTGAAAATGGCATTACGGACATCAGGACATCTCTTACTG GGAGTAGTTCGAATCTATCACAGGAAAGCCAAATACCTTCTTGCAGACTGTAATGAAGCA TTCATTAAGATAAAGATGGCTTTTCGGCCAGGTGTGGTTGACCTGCCTGAGGAAAATCGG GAAGCAGCTTATAATGCCATTACTTTACCTGAAGAATTTCATGACTTTGATCAGCCACTG CCTGACTTAGATGACATCGATGTGGCCCAGCAGTTCAGCTTGAATCAGAGTAGAGTGGAA GAGATAACCATGAGAGAAGAAGTTGGGAACATCAGTATTTTACAAGAAATGATTNTGGTG ATTTGGNAATGGATGATCGTGAGATATGANAGAAGCAGTGCTTTTGAGATGACGACATGT ANTAGCACTACTACTTCTAACCTCTATANATCTGACAGACACCACATCTGATGAGAAATA C >OriGene 3' read for NM_006265 unedited
TTTTNNNNNNNNNNNNNNTTTTTTTTCTGTGNNANCNCGGNNCCGCATTTNANGATCGAG TTTTTTTTTTTTTTTTTTTTTTTTTTACAGCTTATAACACAACTTTTATTAGAAAAGTTA TACATAACATAGCATCAACTATTTTCAAGAACAATATTAAACCCGATAAGCAACAAAAAC CAGACTAACAAAATGTGTAACAAGAAACTAATGACCTTTCTAAAATCAAACATTCAATTA TCTACAATGTCTTTTTACAAACGGGGGAAAACTCCTTGGTTTACAGGCACATCATATTGA ATATANAAAGNCCTGGCAATAGCAATTTATACAATTACACTCTGAAGAACTGAATCATAA AACAGTAATACGAGTTCACAATTTAAAACATTTAACATAATTTTTAAATTATTGGGGGTT TCCCCTGGAAATTTGGGGTTTTAAAAAAAGGGGATTTTTTTTTTTCCCCCACAAGGTTAA AACTTTAAGCCAAAACTTTTGGGGGGAAACTTGCCCCCCAAAAAGGTGAAAAAAATTTTC TGGGAAAAGTAAACACCGCCCCCCGGATTTTTGGGGGGCTTTGGGGGGGGGGAAAAAAAA AAAAACCTTTTTTGGGGGGGAGGTTTTTTTTTCCCCCNNAAAAAACCGGCCCAAGAAGAC GGGGTTTTTTTGGGGGTTTTTTTTTCCCCCCCCCCCTTTTTTTTTTTTCCCCACCCACAA CGGGTGGGTTCCTCCTTGGGGGAAAGAAACCTCTCCCCCCCCCCCCCAAAAAAAAATATA TGAAAAAAGGAGCGTTTTTTTTTTCTCACCCCCCCCTTTTTTTTTTAAAAAAAAAAAAAG GGGCGGGGGGGGGGGGCCCCCCCCCCCCCACGCGGGGGCGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006265 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006265.1, NP_006256.1 |
| RefSeq Size | 3647 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 5885 |
| UniProt ID | O60216 |
| Domains | Rad21_Rec8, Rad21_Rec8_N |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle |
| Gene Summary | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208262 | RAD21 (Myc-DDK-tagged)-Human RAD21 homolog (S. pombe) (RAD21) |
CNY 7,048.00 |
|
| RC208262L1 | Lenti ORF clone of Human RAD21 homolog (S. pombe) (RAD21), Myc-DDK-tagged |
CNY 9,448.00 |
|
| RC208262L2 | Lenti ORF clone of Human RAD21 homolog (S. pombe) (RAD21), mGFP tagged |
CNY 9,448.00 |
|
| RC208262L3 | Lenti ORF clone of Human RAD21 homolog (S. pombe) (RAD21), Myc-DDK-tagged |
CNY 9,448.00 |
|
| RC208262L4 | Lenti ORF clone of Human RAD21 homolog (S. pombe) (RAD21), mGFP tagged |
CNY 9,448.00 |
|
| RG208262 | RAD21 (tGFP-tagged) - Human RAD21 homolog (S. pombe) (RAD21) |
CNY 8,648.00 |
|
| SC322657 | RAD21 (untagged)-Human RAD21 homolog (S. pombe) (RAD21) |
CNY 7,056.00 |
