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REST (NM_005612) Human Untagged Clone
CAT＃: SC116634
REST (untagged)-Human RE1-silencing transcription factor (REST), transcript variant 1
CNY 11,376.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DFNA27; GINGF5; HGF5; NRSF; WT6; XBR |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005612 edited
ACCGAGGAAGGCCGAATACAGTTATGGCCACCCAGGTAATGGGGCAGTCTTCTGGAGGAG GAGGGCTGTTTACCAGCAGTGGCAACATTGGAATGGCCCTGCCTAACGACATGTATGACT TGCATGACCTTTCCAAAGCTGAACTGGCCGCACCTCAGCTTATTATGCTGGCAAATGTGG CCTTAACTGGGGAAGTAAATGGCAGCTGCTGTGATTACCTGGTCGGTGAAGAAAGACAGA TGGCAGAACTGATGCCGGTTGGGGATAACAACTTTTCAGATAGTGAAGAAGGAGAAGGAC TTGAAGAGTCTGCTGATATAAAAGGTGGACCTCATGGACTGGAAAACATGGAACTGAGAA GTTTGGAACTCAGCGTCGTAGAACCTCAGCCTGTATTTGAGGCATCAGGTGCTCCAGATA TTTACAGTTCAAATAAAGATCTTCCCCCTGAAACACCTGGAGCGGAGGACAAAGGCAAGA GCTCGAAGACCAAACCCTTTCGCTGTAAGCCATGCCAATATGAAGCAGAATCTGAAGAAC AGTTTGTGCATCACATCAGAGTTCACAGTGCTAAGAAATTTTTTGTGGAAGAGAGTGCAG AGAAGCAGGCAAAAGCCAGGGAATCTGGCTCTTCCACTGCAGAAGAGGGAGATTTCTCCA AGGGCCCCATTCGCTGTGACCGCTGCGGCTACAATACTAATCGATATGATCACTATACAG CACACCTGAAACACCACACCAGAGCTGGGGATAATGAGCGAGTCTACAAGTGTATCATTT GCACATACACAACAGTGAGCGAGTATCACTGGAGGAAACATTTAAGAAACCATTTTCCAA GGAAAGTATACACATGTGGAAAATGCAACTATTTTTCAGACAGAAAAAACAATTATGTTC AGCATGTTAGAACTCATACAGGAGAACGCCCATATAAATGTGAACTTTGTCCTTACTCAA GTTCTCAGAAGACTCATCTAACTAGACATATGCGTACTCATTCAGGTGAGAAGCCATTTA AATGTGATCAGTGCAGTTATGTGGCCTCTAATCAACATGAAGTAACCCGCCATGCAAGAC AGGTTCACAATGGGCCTAAACCTCTTAATTGCCCACACTGTGATTACAAAACAGCAGATA GAAGCAACTTCAAAAAACATGTAGAGCTACATGTGAACCCACGGCAGTTCAATTGCCCTG TATGTGACTATGCAGCTTCCAAGAAGTGTAATCTACAGTATCACTTCAAATCTAAGCATC CTACTTGTCCTAATAAAACAATGGATGTCTCAAAAGTGAAACTAAAGAAAACCAAAAAAC GAGAGGCTGACTTGCCTGATAATATTACCAATGAAAAAACAGAAATAGAACAAACAAAAA TAAAAGGGGATGTGGCTGGAAAGAAAAATGAAAAGTCCGTCAAAGCAGAGAAAAGAGATG TCTCAAAAGAGAAAAAGCCTTCTAATAATGTGTCAGTGATCCAGGTGACTACCAGAACTC GAAAATCAGTAACAGAGGTGAAAGAGATGGATGTGCATACAGGAAGCAATTCAGAAAAAT TCAGTAAAACTAAGAAAAGCAAAAGGAAGCTGGAAGTTGACAGCCATTCTTTACATGGTC CTGTGAATGATGAGGAATCTTCAACAAAAAAGAAAAAGAAGGTAGAAAGCAAATCCAAAA ATAATAGTCAGGAAGTGCCAAAGGGTGACAGCAAAGTGGAGGAGAATAAAAAGCAAAATA CTTGCATGAAAAAAAGTACAAAGAAGAAAACTCTGAAAAATAAATCAAGTAAGAAAAGCA GTAAGCCTCCTCAGAAGGAACCTGTTGAGAAGGGATCTGCTCAGATGGACCCTCCTCAGA TGGGGCCTGCTCCCACAGAGGCGGTTCAGAAGGGGCCCATTCAGGTGGAGCCGCCACCTC CCATGGAGCATGCTCAGATGGAGGGTGCCCAGATACGGCCTGCTCCTGACGAGCCTGTTC AGATGGAGGTGGTTCAGGAGGGGCCTGCTCAGAAGGAGCTGCTGCCTCCCGTGGAGCCTG CTCAGATGGTGGGTGCCCAAATTGTACTTGCTCACATGGAGCTGCCTCCTCCCATGGAGA CTGCTCAGACGGAGGTTGCCCAAATGGGGCCTGCTCCCATGGAACCTGCTCAGATGGAGG TTGCCCAGGTAGAATCTGCTCCCATGCAGGTGGTCCAGAAGGAGCCTGTTCAGATGGAGC TGTCTCCTCCCATGGAGGTGGTCCAGAAGGAGCCTGTTCAGATAGAGCTGTCTCCTCCCA TGGAGGTGGTCCAGAAGGAACCTGTTAAGATAGAGCTGTCTCCTCCCATAGAGGTGGTCC AGAAGGAGCCTGTTCAGATGGAGTTGTCTCCTCCCATGGGGGTGGTTCAGAAGGAGCCTG CTCAGAGGGAGCCACCTCCTCCCAGAGAGCCTCCCCTTCACATGGAGCCAATTTCCAAAA AGCCTCCTCTCCGAAAAGATAAAAAGGAAAAGTCTAACATGCAGAGTGAAAGGGCACGGA AGGAGCAAGTCCTTATTGAAGTTGGCTTAGTGCCTGTTAAAGATAGCTGGCTTCTAAAGG AAAGTGTAAGCACAGAGGATCTCTCACCACCATCACCACCACTGCCAAAGGAAAATTTAA GAGAAGAGGCATCAGGAGACCAAAAATTACTCAACACAGGTGAAGGAAATAAAGAAGCCC CTCTTCAGAAAGTAGGAGCAGAAGAGGCAGATGAGAGCCTACCTGGTCTTGCTGCTAATA TCAACGAATCTACCCATATTTCATCCTCTGGACAAAACTTGAATACGCCAGAGGGTGAAA CTTTAAATGGTAAACATCAGACTGACAGTATAGTTTGTGAAATGAAAATGGACACTGATC AGAACACAAGAGAGAATCTCACTGGTATAAATTCAACAGTTGAAGAACCAGTTTCACCAA TGCTTCCCCCTTCAGCAGTAGAAGAACGTGAAGCAGTGTCCAAAACTGCACTGGCATCAC CTCCTGCTACAATGGCAGCAAATGAGTCTCAGGAAATTGATGAAGATGAAGGCATCCACA GCCATGAAGGAAGTGACCTAAGTGACAACATGTCAGAGGGTAGTGATGATTCTGGATTGC ATGGGGCTCGGCCAGTTCCACAAGAATCTAGCAGAAAAAATGCAAAGGAAGCGTTGGCAG TCAAAGCGGCTAAGGGAGATTTTGTTTGTATCTTCTGTGATCGTTCTTTCAGAAAGGGAA AAGATTACAGCAAACACCTCAATCGCCATTTGGTTAATGTGTACTATCTTGAAGAAGCAG CTCAAGGGCAGGAGTAATGAAACTTTGAACAAGGTTTCAGTTCTTAGTTTGTAAGGTATA TTACATTTTATATTCATTTATGATAGCAGACAACCTTTTAAGATTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005612 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005612.1, NP_005603.1 |
| RefSeq Size | 6635 bp |
| RefSeq ORF | 3267 bp |
| Locus ID | 5978 |
| UniProt ID | Q13127 |
| Domains | zf-C2H2 |
| Protein Families | Transcription Factors |
| Protein Pathways | Huntington's disease |
| Gene Summary | This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211570 | REST (Myc-DDK-tagged)-Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 11,456.00 |
|
| RC211570L1 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, Myc-DDK-tagged |
CNY 13,856.00 |
|
| RC211570L2 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, mGFP tagged |
CNY 13,856.00 |
|
| RC211570L3 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC211570L4 | Lenti ORF clone of Human RE1-silencing transcription factor (REST), transcript variant 1, mGFP tagged |
CNY 13,856.00 |
|
| RG211570 | REST (tGFP-tagged) - Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 13,056.00 |
|
| SC318496 | REST (untagged)-Human RE1-silencing transcription factor (REST), transcript variant 1 |
CNY 11,464.00 |
