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Lysyl tRNA synthetase (KARS) (NM_005548) Human Untagged Clone
CAT＃: SC116645
KARS (untagged)-Human lysyl-tRNA synthetase (KARS), transcript variant 2
CNY 4,456.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMTRIB; DEAPLE; DFNB89; KARS; KARS2; KRS; LEPID |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116645 sequence for NM_005548 edited (data generated by NextGen Sequencing)
ATGGCGGCCGTGCAGGCGGCCGAGGTGAAAGTGGATGGCAGCGAGCCGAAACTGAGCAAG AATGAGCTGAAGAGACGCCTGAAAGCTGAGAAGAAAGTAGCAGAGAAGGAGGCCAAACAG AAAGAGCTCAGTGAGAAACAGCTAAGCCAAGCCACTGCTGCTGCCACCAACCACACCACT GATAATGGTGTGGGTCCTGAGGAAGAGAGCGTGGACCCAAATCAATACTACAAAATCCGC AGTCAAGCAATTCATCAGCTGAAGGTCAATGGGGAAGACCCATACCCACACAAGTTCCAT GTAGACATCTCACTCACTGACTTCATCCAAAAATATAGTCACCTGCAGCCTGGGGATCAC CTGACTGACATCACCTTAAAGGTGGCAGGTAGGATCCATGCCAAAAGAGCTTCTGGGGGA AAGCTCATCTTCTATGATCTTCGAGGAGAGGGGGTGAAGTTGCAAGTCATGGCCAATTCC AGAAATTATAAATCAGAAGAAGAATTTATTCATATTAATAACAAACTGCGTCGGGGAGAC ATAATTGGAGTTCAGGGGAATCCTGGTAAAACCAAGAAGGGTGAGCTGAGCATCATTCCG TATGAGATCACACTGCTGTCTCCCTGTTTGCATATGTTACCTCATCTTCACTTTGGCCTC AAAGACAAGGAAACAAGGTATCGCCAGAGATACTTGGACTTGATCCTGAATGACTTTGTG AGGCAGAAATTTATCATCCGCTCTAAGATCATCACATATATAAGAAGTTTCTTAGATGAG CTGGGATTCCTAGAGATTGAAACTCCCATGATGAACATCATCCCAGGGGGAGCCGTGGCC AAGCCTTTCATCACTTATCACAACGAGCTGGACATGAACTTATATATGAGAATTGCTCCA GAACTCTATCATAAGATGCTTGTGGTTGGTGGCATCGACCGGGTTTATGAAATTGGACGC CAGTTCCGGAATGAGGGGATTGATTTGACGCACAATCCTGAGTTCACCACCTGTGAGTTC TACATGGCCTATGCAGACTATCACGATCTCATGGAAATCACGGAGAAGATGGTTTCAGGG ATGGTGAAGCATATTACAGGCAGTTACAAGGTCACCTACCACCCAGATGGCCCAGAGGGC CAAGCCTACGATGTTGACTTCACCCCACCCTTCCGGCGAATCAACATGGTAGAAGAGCTT GAGAAAGCCCTGGGGATGAAGCTGCCAGAAACGAACCTCTTTGAAACTGAAGAAACTCGC AAAATTCTTGATGATATCTGTGTGGCAAAAGCTGTTGAATGCCCTCCACCTCGGACCACA GCCAGGCTCCTTGACAAGCTTGTTGGGGAGTTCCTGGAAGTGACTTGCATCAATCCTACA TTCATCTGTGATCACCCACAGATAATGAGCCCTTTGGCTAAATGGCACCGCTCTAAAGAG GGTCTGACTGAGCGCTTTGAGCTGTTTGTCATGAAGAAAGAGATATGCAATGCGTATACT GAGCTGAATGATCCCATGCGGCAGCGGCAGCTTTTTGAAGAACAGGCCAAGGCCAAGGCT GCAGGTGATGATGAGGCCATGTTCATAGATGAAAACTTCTGTACTGCCCTGGAATATGGG CTGCCCCCCACAGCTGGCTGGGGCATGGGCATTGATCGAGTCGCCATGTTTCTCACGGAC TCCAACAACATCAAGGAAGTACTTCTGTTTCCTGCCATGAAACCCGAAGACAAGAAGGAG AATGTAGCAACCACTGATACACTGGAAAGCACAACAGTTGGCACTTCTGTCTAG Clone variation with respect to NM_005548.2 >OriGene 5' read for NM_005548 unedited
TTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGTACTATCC TCCTTACTTTTGGGTCGGGCCCTCCGGGAAGAGGCGGCCGTGCAGGCGGCCGAGGTGAAA GTGGATGGCAGCGAGCCGAAACTGAGCAAGAATGAGCTGAAGAGACGCCTGAAAGCTGAG AAGAAAGTAGCAGAGAAGGAGGCCAAACAGAAAGAGCTCAGTGAGAAACAGCTAAGCCAA GCCACTGCTGCTGCCACCAACCACACCACTGATAATGGTGTGGGTCCTGAGGAAGAGAGC GTGGACCCAAATCAATACTACAAAATCCGCAGTCAAGCAATTCATCAGCTGAAGGTCAAT GGGGAAGACCCATACCCACACAAGTTCCATGTAGACATCTCACTCACTGACTTCATCCAA AAATATAGTCACCTGCAGCCTGGGGATCACCTGACTGACATCACCTTAAAGGTGGCAGGT AGGATCCATGCCAAAAGAGCTTCTGGGGGAAAGCTCATCTTCTATGATCTTCGAGGAGAG GGGGTGAAGTTGCAAGTCATGGCCAATTCCAGAAATTATAAATCAGAAGAAGAATTTATT CATATTAATAACAAACTGCGTCGGGGAGACATAATTGGAGTTCAGGGGAATCCTGGTAAA ACCAAGAAGGGTGAGCTGAGCATCATTCCGTATGAGATCACACTGCTGTCNTCCCTGTTG CATATGTTACCTCATCTTCACTTTGGCCNTCAAGACAGGGAAACANGGTATCGCCAGAGA TACTTGGACTTGATCCTGAATGACTTTGTGAGGCAGAAATTATCATCCGCTCTAAGACAT CACATATATAAGAAGTTNCTTAGATGAGCTGGGATTCCCTAGAGATGAACTCCCATGATG ACATCATCCCAGGGGNACGCCGTGGCCAC >OriGene 3' read for NM_005548 unedited
AGAGTCGAGTTTTTTTTTTTTTTTTTTGGTAACAGGATTAAAAAGAAATTTTTAATTCCT TGTCTCTCTTCTGATGGCTGAACAGAACTGCGGTGTCAAATGGAAAGCAGCACACAAGAA TTCCCTTGCAGACCTTGATCTTTCGCAGAAATGCAAAGACGCCTGAGTTATACAACTTGC AATTATTATTTTCTAGACAGAAGTGCCAACTGTTGTGCTTTCCAGTGTATCAGTGGTTGC TACATTCTCCTTCTTGTCTTCGGGTTTCATGGCAGGAAACAGAAGTACTTCCTTGATGTT GTTGGAGTCCGTGAGAAACATGGCGACTCGATCAATGCCCATGCCCCAGCCAGCTGTGGG GGGCAGCCCATATTCCAGGGCAGTACAGAAGTTTTCATCTATGAACATGGCCTCATCATC ACCTGCAGCCTTGGCCTTGGCCTGTTCTTCAAAAAGCTGCCGCTGCCGCATGGGATCATT CAGCTCAGTATACGCATTGCATATCTCTTTCTTCATGACAAACAGCTCAAAGCGCTCAGT CAGACCCTCTTTAGAGCCGTGCCATTTAGCCAAAGGGCTCATTATCTGTGGGTGATCACA GATGAATGTANGATTGATGCAAGTTACTTCCCAGAACTCCCAACAAGCTTGTCAAGAGCC TGGCTGTGGTCGAGGGGGAGGCATCCACAGCTTTTGCCAACAGATATATCAAAAATTTGC GAGTTCCTCAGTTCAAGGAGGTNGTTCTTGGAGTTAATTCCAAGGCTTTTTAAGCTTTTA CATGTGATTCCCGCAGGTGGGGGAATAACATGGAGGCTGGCCTTTGGCCCTCGGTGGAAG GACCTGAACGCCGGAAAGCTTACCTCCCGAACCTTTTTCGGATTCATGAACGGAATCCGC AGCCTTAAACCCGGGGGACCGGTTGGGAAAAACCCTTTTGGAAGGGCCTTATAACCGGAG CCCCCCAACTTTGAAGCTGGGACCTTTAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005548 |
| Insert Size | 2050 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005548.1, NP_005539.1 |
| RefSeq Size | 1997 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 3735 |
| UniProt ID | Q15046 |
| Domains | tRNA-synt_2, tRNA_anti |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| Gene Summary | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in an upstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Hepatitis B Virus X Protein Upregulates hELG1/ ATAD5 Expression through E2F1 in Hepatocellular Carcinoma
,null,
International Journal of Biological Sciences
,PubMed ID 26722215
[KARS1]
|
|
Sense-antisense gene-pairs in breast cancer and associated pathological pathways
,null,
Oncotarget
,PubMed ID 26517092
[KARS1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200311 | KARS (Myc-DDK-tagged)-Human lysyl-tRNA synthetase (KARS), transcript variant 2 |
CNY 4,448.00 |
|
| RC200311L1 | Lenti ORF clone of Human lysyl-tRNA synthetase (KARS), transcript variant 2, Myc-DDK-tagged |
CNY 6,848.00 |
|
| RC200311L2 | Lenti ORF clone of Human lysyl-tRNA synthetase (KARS), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC200311L3 | Lenti ORF clone of Human lysyl-tRNA synthetase (KARS), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200311L4 | Lenti ORF clone of Human lysyl-tRNA synthetase (KARS), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG200311 | KARS (tGFP-tagged) - Human lysyl-tRNA synthetase (KARS), transcript variant 2 |
CNY 6,048.00 |
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