United States
Germany
Japan
United Kingdom
China
SAE1 (NM_005500) Human Untagged Clone
CAT＃: SC116689
SAE1 (untagged)-Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1
CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AOS1; HSPC140; SUA1; UBLE1A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116689 sequence for NM_005500 edited (data generated by NextGen Sequencing)
ATGGTGGAGAAGGAGGAGGCTGGCGGCGGCATTAGCGAGGAGGAGGCGGCACAGTATGAC CGGCAGATCCGCCTGTGGGGACTGGAGGCCCAGAAACGGCTGCGGGCCTCTCGGGTGCTT CTTGTCGGCTTGAAAGGACTTGGGGCTGAAATTGCCAAGAATCTCATCTTGGCAGGAGTG AAAGGACTGACCATGCTGGATCACGAACAGGTAACTCCAGAAGATCCCGGAGCTCAGTTC TTGATTCGTACTGGGTCTGTTGGCCGAAATAGGGCTGAAGCCTCTTTGGAGCGAGCTCAG AATCTCAACCCCATGGTGGATGTGAAGGTGGACACTGAGGATATAGAGAAGAAACCAGAG TCATTTTTCACTCAATTCGATGCTGTGTGTCTGACTTGCTGCTCCAGGGATGTCATAGTT AAAGTTGACCAGATCTGTCACAAAAATAGCATCAAGTTCTTTACAGGAGATGTTTTTGGC TACCATGGATACACATTTGCCAATCTAGGAGAGCATGAGTTTGTAGAGGAGAAAACTAAA GTTGCCAAAGTTAGCCAAGGAGTAGAAGATGGGCCCGACACCAAGAGAGCAAAACTTGAT TCTTCTGAGACAACGATGGTCAAAAAGAAGGTGGTCTTCTGCCCTGTTAAAGAAGCCCTG GAGGTGGACTGGAGCAGTGAGAAAGCAAAGGCTGCTCTGAAGCGCACGACCTCCGACTAC TTTCTCCTTCAAGTGCTCTTAAAGTTCCGTACAGATAAAGGAAGAGATCCCAGTTCTGAT ACATATGAGGAAGATTCTGAGTTGTTGCTCCAGATACGAAATGATGTGCTTGACTCACTG GGTATTAGTCCTGACCTGCTTCCTGAGGACTTTGTCAGGTACTGCTTCTCCGAGATGGCC CCAGTGTGTGCGGTGGTTGGAGGGATTTTGGCACAGGAAATTGTGAAGGCCCTGTCTCAG CGGGACCCTCCTCACAACAACTTCTTCTTCTTCGATGGCATGAAGGGGAATGGGATTGTG GAGTGCCTTGGCCCCAAGTGA Clone variation with respect to NM_005500.2 >OriGene 5' read for NM_005500 unedited
AAATTTGGGATTTTGTAATCCGACTTACTATAGGGNCGGACCGCGCAATTCGGCACGAGG CCGGAGCTGAGGCAGGAAGAGCCGGCGCCATGGTGGAGAAGGAGGAGGCTGGCGGCGGCA TTAGCGAGGAGGAGGCGGCACAGTATGACCGGCAGATCCGCCTGTGGGGACTGGAGGCCC AGAAACGGCTGCGGGCCTCTCGGGTGCTTCTTGTCGGCTTGAAAGGACTTGGGGCTGAAA TTGCCAAGAATCTCATCTTGGCAGGAGTGAAAGGACTGACCATGCTGGATCACGAACAGG TAACTCCAGAAGATCCCGGAGCTCAGTTCTTGATTCGTACTGGGTCTGTTGGCCGAAATA GGGCTGAAGCCTCTTTGGAGCGAGCTCAGAATCTCAACCCCATGGTGGATGTGAAGGTGG ACACTGAGGATATAGAGAAGAAACCAGAGTCATTTTTCACTCAATTCGATGCTGTGTGTC TGACTTGCTGCTCCAGGGATGTCATAGTTAAAGTTGACCAGATCTGTCACAAAAATAGCA TCAAGTTCTTTACAGGAGATGTTTTTGGCTACCATGGATACACATTTGCCAATCTAGGAG AGCATGAGTTTGTAGAGGAGAAAACTAAAGTTGCCAAAGTTAGCCAAGGAGTAGAAGATG GGCCCGACACCAAGAGAGCAAAACTTGATTCTTCTGAGACAACGATGGTCAAAAAGAAGG TGGTCTTCTGCCCTGTTAAAGAAGCCCTGNGAGTGGACTGGAGCAGTGAGAAAGCAAGGC TGCTCTGAAGCGCACGACCTNCGACTACTTTCTCCTTNCAGTGCTCTTAAAGTTCCGTAC AGATNAAGGAAGAGATCCCAGTTCTGATACATATGAAGAAAGATTCTGAGTGNTGCTNCA GATACGAATGATGTGC >OriGene 3' read for NM_005500 unedited
AGAGTCGAGTTTTTTTTTTTTTTTTTTCCTAAAGCAAGTAACTTTATTATCATTCCTTTA AAAAGAACCAAGGAAAATTCACAACATATGTGAAACACAAACAGCTGTGGTTTAGGAGGT AAACAAAGGACCAACATAGCCCTGAAATGCAACAGCCTCTGAGTGACTTGAGCCGCATGT GACTGGGGTTCTGTTAAAAGGGCAGGCTCCTCCCTCCTAGCCCTGAAGCCCCAGGAACCT GCCTTGAAAGACAAGCTCTCTAATACTCAACTTGCAGGGTCTCGCCCTAACATCCAAGAC TTGGTAGCATCTCCTTTCTCCCAAAACCCAGCTGGAACTCAACTAATCCTAAACGAAAAC TCAAGAACAGCACACCAGATGCCACCTGTTGTTTGTCAGGGTCTCAAACTCCAGAGGAAA TGCATTTGCCTGTCATGGTTTTCCTCTCTAAGGGCACCTGTCTGAACTTGGAGCTGTGCT TCCACTGGTGCCTCATGGNGCCTGGGCCTACTCTCCACCTGAATTCACACTTTTCTCTAT AGGTATCAGGTGGGGAATAAGTCCAAGAAGGTGACAAGAGGCTCCCGAGCACAGAGCTTG CTCATTCCTGNCANGCATCTCCCACAATGCCCCANNAGGGCTGCGAGGCCCGGNACAGCA TANGGACAGAGAAAGGGCANAGGGGTGCTTGGAAGTGCTCATCACTCANGGGAGCTGCCA GGCCCCGGATGACANGCANCCTGAACAGTGCTGGAAACAAGACAGNCACCCTGCGCCCCT TGTCNAGCANCTGCTTNTGATGGGAAANCACACGTCGNCACCACCTTCTTCGTGCANGAA ATGNTNTGTATCGGGCCCATGACTCAGTTTNCCTTGCTGGAGATGCCTTCATGAAGAAGG NGACAGGNATACAGNTGGCATGCTGCANTGGCATCTCTGGGCTGCAAAACTTGGNTACTT GGGGCANGACTNACAATCCATTCCTTATGCTCANAAAAAAAATGTGNAGAGAGGTCGCTA AAAGGCTNCAATNCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005500 |
| Insert Size | 2050 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005500.1, NP_005491.1 |
| RefSeq Size | 2119 bp |
| RefSeq ORF | 1041 bp |
| Locus ID | 10055 |
| UniProt ID | Q9UBE0 |
| Domains | ThiF |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] Transcript Variant: This variant (1) encodes the longest isoform (a). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Small-molecule activation of SERCA2a SUMOylation for the treatment of heart failure
,Kho, C;Lee, A;Jeong, D;Oh, JG;Gorski, PA;Fish, K;Sanchez, R;DeVita, RJ;Christensen, G;Dahl, R;Hajjar, RJ;,
Nat Commun
,PubMed ID 26068603
[SAE1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201820 | SAE1 (Myc-DDK-tagged)-Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1 |
CNY 3,656.00 |
|
| RC201820L1 | Lenti ORF clone of Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201820L2 | Lenti ORF clone of Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201820L3 | Lenti ORF clone of Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201820L4 | Lenti ORF clone of Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201820 | SAE1 (tGFP-tagged) - Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1 |
CNY 5,256.00 |
