SEPP1 (SELENOP) (NM_005410) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Synonyms	SELP; SeP; SEPP; SEPP1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC116747 sequence for NM_005410 edited (data generated by NextGen Sequencing) ATGTGGAGAAGCCTGGGGCTTGCCCTGGCTCTCTGTCTCCTCCCATCGGGAGGAACAGAG AGCCAGGACCAAAGCTCCTTATGTAAGCAACCCCCAGCCTGGAGCATAAGAGATCAAGAT CCAATGCTAAACTCCAATGGTTCAGTGACTGTGGTTGCTCTTCTTCAAGCCAGCTGATAC CTGTGCATACTGCAGGCATCTAAATTAGAAGACCTGCGAGTAAAACTGAAGAAAGAAGGA TATTCTAATATTTCTTATATTGTTGTTAATCATCAAGGAATCTCTTCTCGATTAAAATAC ACACATCTTAAGAATAAGGTTTCAGAGCATATTCCTGTTTATCAACAAGAAGAAAACCAA ACAGATGTCTGGACTCTTTTAAATGGAAGCAAAGATGACTTCCTCATATATGATAGATGT GGCCGTCTTGTATATCATCTTGGTTTGCCTTTTTCCTTCCTAACTTTCCCATATGTAGAA GAAGCCATTAAGATTGCTTACTGTGAAAAGAAATGTGGAAACTGCTCTCTCACGACTCTC AAAGATGAAGACTTTTGTAAACGTGTATCTTTGGCTACTGTGGATAAAACAGTTGAAACT CCATCGCCTCATTACCATCATGAGCATCATCACAATCATGGACATCAGCACCTTGGCAGC AGTGAGCTTTCAGAGAATCAGCAACCAGGAGCACCAAATGCTCCTACTCATCCTGCTCCT CCAGGCCTTCATCACCACCATAAGCACAAGGGTCAGCATAGGCAGGGTCACCCAGAGAAC CGAGATATGCCAGCAAGTGAAGATTTACAAGATTTACAAAAGAAGCTCTGTCGAAAGAGA TGTATAAATCAATTACTCTGTAAATTGCCCACAGATTCAGAGTTGGCTCCTAGGAGCTGA TGCTGCCATTGTCGACATCTGATATTTGAAAAAACAGGGTCTGCAATCACCTGACAGTGT AAAGAAAACCTCCCATCTTTATGTAGCTGACAGGGACTTCGGGCAGAGGAGAACATAACT GAATCTTGTCAGTGACGTTTGCCTCCAGCTGCCTGACAAATAAGTCAGCAGCTTATACCC ACAGAAGCCAGTGCCAGTTGACGCTGAAAGAATCAGGCAAAAAAGTGAGAATGACCTTCA AACTAA Clone variation with respect to NM_005410.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_005410 unedited GATCTTGGGATTTTGTAATCCGACTTACTATAGGCGGCCGCGCAATTCGCACGAGGCCAG AGTAAAGCAAANAGAAAGGAAGCAGGCCCGTTGGAAGTGGTTGTGACAACCCCAGCAATG TGGAGAAGCCTGGGGCTTGCCCTGGCTCTCTGTCTCCTCCCATCGGGAGGAACAGAGAGC CAGGACCAAAGCTCCTTATGTAAGCAACCCCCAGCCTGGAGCATAAGAGATCAAGATCCA ATGCTAAACTCCAATGGTTCAGTGACTGTGGTTGCTCTTCTTCAAGCCAGCTGATACCTG TGCATACTGCAGGCATCTAAATTAGAAGACCTGCGAGTAAAACTGAAGAAAGAAGGATAT TCTAATATTTCTTATATTGTTGTTAATCATCAAGGAATCTCTTCTCGATTAAAATACACA CATCTTAAGAATAAGGTTTCAGAGCATATTCCTGTTTATCAACAAGAAGAAAACCAAACA GATGTCTGGACTCTTTTAAATGGAAGCAAAGATGACTTCCTCATATATGATAGATGTGGC CGTCTTGTATATCATCTTGGTTTGCCTTTTTCCTTCCTAACTTTCCCATATGTAGAAGAA GCCATTAAGATTGCTTACTGTGAAAAGAAATGTGGAAACTGCTCTCTCACGACTCTCANA GATGAAGACTTTTGTAAACGTGTATCTTTGGCTACTGTGGATAAAACAGTTGANACTCCA TCGCCTCATTACCATCATGAGCATCATCACAATCATGGACATCAGCACCTTGGCAGCAGT GAGCTTTCAGAGAATCAGNCACCAGGAGCACCAAATGCTCCTACTCATCCTGCNTCTNCA GGCCTTCATCACCACCATAAGCACAAGGGTCAGCATAGGCAGGGNTCACCCAGAGAACCG AGATATGCCCCAGCAGTGAAGATTTACNNAGATTACAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_005410 unedited TAGCTATGAACCCGCGGCCGCAATCTAGGATCGAGNNNTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTAAAGGGTTTTATTGAATTTATTTGGACAAATCCGTACTGTATCC AATTCTGTACTGCATTCTTGCTTAAATAGTATTAACCATAAAGGAGGTCAGGTTTATAGG GTTTGGTTTACCTATTAAACCATCATTGACTATGGAAATACTTACTAAGCATTATAGAGA CAGACAATATTATCTTTCCCCTTATATCTTTTAAAACAGCCACTCAAGTTTTAAAAGAGT ATGATACAACATTAGAGAAAGAAAGATACAAAGGCTTTATTCATGTGTGATAGTAAAAAT CAGGATGAGTCTTAAATATACAAAAGATAAATGGATATTTAAAATAGTTATATATGCTTT TTTAACAAAATATTCACGTGTTAAGTATTTCTGGATCTTAAAATACAAAATCCACTTATT TTATTAGTTAAAAGCAAAAGATTGAAAAGACAAAGTAATATTGTTTTGAAAGATAAGTAA AGAAAAAAATGGAAAGCATGCCTTTGTTGTTCTTCCTCCCATTCTAAACTGCTAATTATC CAACAGAAACCCCTAGGTCATAGTTTACGTTTCTATTCTCATTAAAGCAAATACAACACT GGAAAAAGAAAAAAAAGACCTATTAACCAAAAGCTGCAATCACCTTTCAGTTGCTCCATC ATAAAAAATATGGTTTGAGTCAATATTTCTATGACATAAAATTTAAAATCTCGAACCCCA ATTAAGCAAATTTCTCCATGTTTGCACAAATCCTAANTTCTATTTTTGGCTCCACTAATT GGCGAGTTTAAAAAAACGCCTGGAAATGAAAATGTGTCTACACTAAATGGCGGAGATGTT CTATTTCAAAAATAAGGTTGGAAGCGCTTTCTAATTTTTTGGCTCGATCTTTCAGGGCAA ATGCG
Restriction Sites	NotI-NotI
ACCN	NM_005410
Insert Size	2080 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation	This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_005410.2, NP_005401.3
RefSeq Size	2164 bp
Locus ID	6414
UniProt ID	P49908
Protein Families	Secreted Protein
Gene Summary	This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in human), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. The use of alternative polyadenylation sites, one located in between the two SECIS elements, results in two populations of mRNAs containing either both (predominant) or just the upstream SECIS element (PMID:27881738). Alternatively spliced transcript variants have also been found for this gene. [provided by RefSeq, Oct 2018] Transcript Variant: This variant (1, also known as Sepp1a) represents the predominant transcript and encodes isoform 1. Variants 1 and 2 encode the same isoform.

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