Histone H1.2 (HIST1H1C) (NM_005319) Human Untagged Clone

CAT#: SC116823

HIST1H1C (untagged)-Human histone cluster 1, H1c (HIST1H1C)



  "NM_005319" in other vectors (6)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-HIST1H1C Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms H1.2; H1C; H1F2; H1s-1; HIST1H1C
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116823 sequence for NM_005319 edited (data generated by NextGen Sequencing)
ATGTCCGAGACTGCTCCTGCCGCTCCCGCTGCCGCGCCTCCTGCGGAGAAGGCCCCTGTA
AAGAAGAAGGCGGCCAAAAAGGCTGGGGGTACGCCTCGTAAGGCGTCTGGTCCCCCGGTG
TCAGAGCTCATCACCAAGGCTGTGGCCGCCTCTAAAGAGCGTAGCGGAGTTTCTCTGGCT
GCTCTGAAAAAAGCGTTGGCTGCCGCCGGCTATGATGTGGAGAAAAACAACAGCCGTATC
AAACTTGGTCTCAAGAGCCTGGTGAGCAAGGGCACTCTGGTGCAAACGAAAGGCACCGGT
GCTTCTGGCTCCTTTAAACTCAACAAGAAGGCAGCCTCCGGGGAAGCCAAGCCCAAGGTT
AAAAAGGCGGGCGGAACCAAACCTAAGAAGCCAGTTGGGGCAGCCAAGAAGCCCAAGAAG
GCGGCTGGCGGCGCAACTCCGAAGAAGAGCGCTAAGAAAACACCGAAGAAAGCGAAGAAG
CCGGCCGCGGCCACTGTAACCAAGAAAGTGGCTAAGAGCCCAAAGAAGGCCAAGGTTGCG
AAGCCCAAGAAAGCTGCCAAAAGTGCTGCTAAGGCTGTGAAGCCCAAGGCCGCTAAGCCC
AAGGTTGTCAAGCCTAAGAAGGCGGCGCCCAAGAAGAAATAG

Clone variation with respect to NM_005319.3
>OriGene 5' read for NM_005319 unedited
GGGGGTTTAAATTTGTATACGACTCATATAGGCGGCCGCGNATTCGGCACGAGGGCCACT
TGTACCCGAGTTTTTGATTCTCAACATGTCCGAGACTGCTCCTGCCGCTCCCGCTGCCGC
GCCTCCTGCGGAGAAGGCCCCTGTAAAGAAGAAGGCGGCCAAAAGGCTGGGGGTACGCCT
CGTAAGGCGTCTGGTCCCCCGGTGTCAGAGCTCATCACCAAGGCTGTGGCCGCCTCTAAA
GAGCGTAGCGGAGTTTCTCTGGCTGCTCTGAAAAAAGCGTTGGCTGCCGCCGGCTATGAT
GTGGAGAAAAACAACAGCCGTATCAAACTTGGTCTCAAGAGCCTGGTGAGCAAGGGCACT
CTGGTGCAAACGAAAGGCACCGGTGCTTCTGGCTCCTTTAAACTCAACAAGAAGGCAGCC
TCCGGGGAAGCCAAGCCCAAGGTTAAAAAGGCGGGCGGAACCAAACCTAAGAAGCCAGTT
GGGGCAGCCAAGAAGCCCAAGAAGGCGGCTGGCGGCGCAACTCCGAAGAAGAGCGCTAAG
AAAACACCGAAGAAAGCGAAGAAGCCGGCCGCGGCCACTGTAACCAAGAAAGTGGCTAAG
AGCCCAAAGAAGGCCAAGGTTGCGAAGCCCAAGAAAGCTGCCAAAAGTGCTGCTAAGGCT
GTGAAGCCCAAGGCCGCTAAGCCCAAGGTTGTCAAGCCTAAGAAGGCGGCGCCCAAGAAG
AAATAGGCGAACGCCTACTTCTAAAACCCAAAGGCTCTTTTCAGAGCCACCACTGATCTC
AATAAAAGAGCTGGATAATTTCTTTACAAAAAANAAAAAAAAAAACTCGACTCTAGATTT
GCGCCGCGGTCATAGCTGTTTCCTGAACAGACCCCGGNTGGGATCCCTGTGACCA
>OriGene 3' read for NM_005319 unedited
GGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAAAAAAATATCC
AGCTCTTTTATTGAGATCAGTGGTGGCTCTGAAAAGACCTTTTGGGTTTTAGAAGTAGGC
GTTCGCCTATTTCTTCTTGGGCGCCGCCTTCTTAGGCTTGACAACCTTGGGCTTAGCGGC
CTTGGGCTTCACAGCCTTAGCAGCACTTTTGGCAGCTTTCTTGGGCTTCGCAACCTTGGC
CTTCTTTGGGCTCTTAGCCACTTTCTTGGTTACAGTGGCCGCGGCCGGCTTCTTCGCTTT
CTTCGGTGTTTTCTTAGCGCTCTTCTTCGGAGTTGCGCCGCCAGCCGCCTTCTTGGGCTT
CTTGGCTGCCCCAACTGGCTTCTTAGGTTTGGTTCCGCCCGCCTTTTTAACCTTGGGCTT
GGCTTCCCCGGAGGCTGCCTTCTTGTTGAGTTTAAAGGAGCCAGAAGCACCGGTGCCTTT
CGTTTGCACCAGAGTGCCCTTGCTCACCAGGCTCTTGAGACCAAGTTTGATACGGCTGTT
GTTTTTCTCCACATCATAGCCGGCGGCAGCCAACGCTTTTTTCAGAGCAGCCAGAGAAAC
TCCGCTACGCTCTTTAGAGGCGGCCACAGCCTTGGTGATGAGCTCTGACACCGGGGGACC
AGACGCCTTACGAGGCGTACCCCCAGCCTTTTTGGCCGCCTTCTTCTTTACAGGGGCCCT
TCTCCGCAAGAAGCGCCGCAACGGGAGCCGCCAGAGCANTCTCGGACATGTTGAGAACAA
AAACTCGGGTACAAGTGGCCTTCGTGCCGAATTCCGGCCGCCCTAAATGAAGCCGATACA
AAATCTGACGGTCACTAACGGACCCTGCTTATTAGACCTCCACCGACCCGCCTACCGCCA
TTTGGTCACGGGGCGG
Restriction Sites NotI-NotI     
ACCN NM_005319
Insert Size 820 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005319.3, NP_005310.1
RefSeq Size 732 bp
RefSeq ORF 642 bp
Locus ID 3006
UniProt ID P16403
Domains linker_histone
Gene Summary Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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