H3.3B (H3F3B) (NM_005324) Human Untagged Clone

CAT#: SC116824

H3F3B (untagged)-Human H3 histone, family 3B (H3.3B) (H3F3B)



  "NM_005324" in other vectors (6)

CNY 1,200.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-H3F3B(Histone H3) antibody, Loading control
    • 100 ul

CNY 6,281.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms H3-3A; H3.3B; H3F3B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116824 sequence for NM_005324 edited (data generated by NextGen Sequencing)
ATGGCCCGAACCAAGCAGACTGCTCGTAAGTCCACCGGTGGGAAAGCCCCCCGCAAACAG
CTGGCCACGAAAGCCGCCAGGAAAAGCGCTCCCTCTACCGGCGGGGTGAAGAAGCCTCAT
CGCTACAGGCCCGGGACCGTGGCGCTTCGAGAGATTCGTCGTTATCAGAAGTCGACCGAG
CTGCTCATCCGGAAGCTGCCCTTCCAGAGGTTGGTGAGGGAGATCGCGCAGGATTTCAAA
ACCGACCTGAGGTTTCAGAGCGCAGCCATCGGTGCGCTGCAGGAGGCTAGCGAAGCGTAC
CTGGTGGGTCTGTTCGAAGATACCAACCTGTGTGCCATCCACGCTAAGAGAGTCACCATC
ATGCCCAAAGACATCCAGTTGGCTCGCCGGATACGGGGAGAGAGAGCTTAA

Clone variation with respect to NM_005324.3
>OriGene 5' read for NM_005324 unedited
NGTGTGCACCATTATGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGT
TTTTCGCTCGTCGACTGCGGCTCTTCCTCGGGCAGCGGAAGCGGCGCGGCGGTCGGAGAA
GTGGCCTAAAACTTCGGCGTTGGGTGAAAGAAAATGGCCCGAACCAAGCAGACTGCTCGT
AAGTCCACCGGTGGGAAAGCCCCCATGCAAACAGCTGGCCACGAAAGCCGCCAGGAAAAG
CGCTCCCTCTACCGGCGGGGTGAAGAAGCCTCATCGCTACAGGCCCGGGACCGTGGCGCT
TCGAGAGATTCGTCGTTATCAGAAGTCGATCCGAGCTGCTCATCCGGAAGCTGCCCTTCC
AGAGGTTGGTGAGGGAGATCGCGCAGGATTTCAAAACCGACCTGAGGTTTCAGAGCGCAG
CCATCGGTGCGCTGCAGGAGGCTAGCGAAGCGTACCTATGTGGGTCTGTTCGAAGATACC
AACCTGTGTGCCATCCACGCTAAGAGAGTCACCATCATGCCCAAATACATCCAGTTGGCT
CGCCGGATACGGNGAGAGAGAGCTTAAGTGAAGGCAGTTTTTATGGCGTTTTGTAGTAAA
TTCTGTAAAATACTTTGGTTTAATTTGTGACTNTTTTTGTAAGAAATTGTTTATAATATG
TTGCATTTGTACTTAAGTCATTCCATCTTTCACTCANGATGAATGCGAAAAGTGACTGTT
CACAGACCTCAGTGATGTGAGCACTGTTGCTCANGAGTGACAAGTTGCTAATATGCAGAA
GGGATGGGTGATACTTCTTGCTTCTCATGATGCATGTTTCTGTATGTTAATGACTTGTTG
GGTAGCTATTAAGTACTAGAGTTGATTAATGTGTACAGGGTCCTTTTGCATANAACATTT
ATGATCTGATCCAGTGTTACTACAATGGGCTGTTAATCTGACC
>OriGene 3' read for NM_005324 unedited
CACCACCCCCAAAACATCCTTCCTTTTCCCTTTTATTTGCCCGCGGCCGCATTCTANGAT
CGGTTTTTTTTTTTTTTTTTTGGGAAAACAAGTTTATTTGTAAATTTAGTCAACATACAT
AATTGACCTAAAAACTTCAGTAAATTTTAAAACCACTTGCGAAGGCCATCTCTATAAAAA
TGATTTTCCCAGGACAGTAACCAGATGTAACCTAACCCAACACCATCTTAATTGGCAGAG
GTTCAGTGGGCTGGAGTTTTGTGCTCCTCCCCCACACCAAGTTTTTATAATACAAATGCC
ACAAGAAAAAGAACTTCGGTACTGTTTCCTCAGCAGAGGAGAAAAACTCAACCTAGTTAT
GAGACCAACCACACAACACAATGAAAAGCTGCACTAACTAGTTCAGAATGTTAGTTAAGA
TGATGCTGGTGTGAATAACTCGCTTTTTCTAGAGCCCTTATAAATAAAATCCCCCAGTTA
GTGTTTGCATTATCAGCTACAGGGTTAACATGTGGTACAATGAGGACTTATGCAAGGCAT
AAATGCGCATAGCATTTTACTACTATGAGAACAAGTGCAGTCAGAAGAAAACCACTGGAC
TCTAAATTACACCCACCTTAATGACCAGACTCCCCACCACTTGTGAATGTACAACACTCC
ACTTTAAAAACGTCGATCCTACCATATATAAAATAGCTATTATAACTGCACATACTGCAT
TCTATTACCGCCAGGCTTACTTTTTTTTTTTTTTAAAGGAAAACTGTAAGTCACCCTGCG
GGTAAAACCTCGTACTTTCACCCCTTGAAAAAGCCCAACTTTTTTTCACAGGGATGTATG
GCCCACATTTACCGCCCCCATTGGTAAACCCTGGGATTCAATCATAACACCTTTTTTTTG
GCAAAGGACCCTGGAAAATTTTACAACCCTTAGACCCTTCATAG
Restriction Sites NotI-NotI     
ACCN NM_005324
Insert Size 1730 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005324.3, NP_005315.1
RefSeq Size 2753 bp
RefSeq ORF 411 bp
Locus ID 3021
UniProt ID P84243
Domains H3, histone
Protein Pathways Systemic lupus erythematosus
Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17. [provided by RefSeq, Oct 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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