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DNase I (DNASE1) (NM_005223) Human Untagged Clone
CAT＃: SC116856
DNASE1 (untagged)-Human deoxyribonuclease I (DNASE1)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DNL1; DRNI |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005223, the custom clone sequence may differ by one or more nucleotides
ATGAGGGGCATGAAGCTGCTGGGGGCGCTGCTGGCACTGGCGGCCCTACTGCAGGGGGCCGTGTCCCTGA AGATCGCAGCCTTCAACATCCAGACATTTGGGGAGACCAAGATGTCCAATGCCACCCTCGTCAGCTACAT TGTGCAGATCCTGAGCCGCTATGACATCGCCCTGGTCCAGGAGGTCAGAGACAGCCACCTGACTGCCGTG GGGAAGCTGCTGGACAACCTCAATCAGGATGCACCAGACACCTATCACTACGTGGTCAGTGAGCCACTGG GACGGAACAGCTATAAGGAGCGCTACCTGTTCGTGTACAGGCCTGACCAGGTGTCTGCGGTGGACAGCTA CTACTACGATGATGGCTGCGAGCCCTGCGGGAACGACACCTTCAACCGAGAGCCAGCCATTGTCAGGTTC TTCTCCCGGTTCACAGAGGTCAGGGAGTTTGCCATTGTTCCCCTGCATGCGGCCCCGGGGGACGCAGTAG CCGAGATCGACGCTCTCTATGACGTCTACCTGGATGTCCAAGAGAAATGGGGCTTGGAGGACGTCATGTT GATGGGCGACTTCAATGCGGGCTGCAGCTATGTGAGACCCTCCCAGTGGTCATCCATCCGCCTGTGGACA AGCCCCACCTTCCAGTGGCTGATCCCCGACAGCGCTGACACCACAGCTACACCCACGCACTGTGCCTATG ACAGGATCGTGGTTGCAGGGATGCTGCTCCGAGGCGCCGTTGTTCCCGACTCGGCTCTTCCCTTTAACTT CCAGGCTGCCTATGGCCTGAGTGACCAACTGGCCCAAGCCATCAGTGACCACTATCCAGTGGAGGTGATG CTGAAGTGA >OriGene 5' read for NM_005223 unedited
TTCCCCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA ATTCGGCACGAGGCGCACTGCCTGTGCAGGTCCGGAGCCCAGCAGCACTGCCAGGGCCTT GAAGTGCTTCTTCAGAGACCTTTCTTCATAGACTACTTTTTTTTCTTTAAGCAGCAAAAG GAGAAAATTGTCATCAAAGGATATTCCAGATTCTTGACAGCATTCTCGTCATCTCTGAGG ACATCACCATCATCTCAGGATGAGGGGCATGAAGCTGCTGGGGGCGCTGCTGGCACTGGC GGCCCTACTGCAGGGGGCCGTGTCCCTGAAGATCGCAGCCTTCAACATCCAGACATTTGG GGAGACCAAGATGTCCAATGCCACCCTCGTCAGCTACATTGTGCAGATCCTGAGCCGCTA TGACATCGCCCTGGTCCAGGAGGTCAGAGACAGCCACCTGACTGCCGTGGGGAAGCTGCT GGACAACCTCAATCAGGATGCACCAGACACCTATCACTACGTGGTCAGTGAGCCACTGGG ACGGAACAGCTATAAGGAGCGCTACCTGTTCGTGTACAGGCCTGACCCAGTGTCTGCGGT GGACAGCTACTACTACGATGATGGCTGCGAGCCCTGCGGGAACGACACCTTCAACCGAGA GCCAGCCATTGTCAGGNTCTTCTCCCGGNTCACAGGGCCCAGGCAGCGNTTCCTTGTANG ACGTCATGTTGATGGGCGACTTCATGCGGGCTGAGCTATGTGAGACCCTCCAGGGGTCAT CATCCGCCTGGGGACAGCCCCACCTTCAGGGGTGATCCCGAAGGGCTGAACCCAGTTCAC CCACCCTGGCCTATACAGGAACGGGTTGAGGGATCTGTCTAGGCCCCCTTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005223 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005223.3, NP_005214.2 |
| RefSeq Size | 3108 bp |
| RefSeq ORF | 849 bp |
| Locus ID | 1773 |
| UniProt ID | P24855 |
| Domains | Exo_endo_phos, DNaseIc |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Gene Summary | This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205927 | DNASE1 (Myc-DDK-tagged)-Human deoxyribonuclease I (DNASE1) |
CNY 3,600.00 |
|
| RC205927L1 | Lenti ORF clone of Human deoxyribonuclease I (DNASE1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC205927L2 | Lenti ORF clone of Human deoxyribonuclease I (DNASE1), mGFP tagged |
CNY 5,890.00 |
|
| RC205927L3 | Lenti ORF clone of Human deoxyribonuclease I (DNASE1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205927L4 | Lenti ORF clone of Human deoxyribonuclease I (DNASE1), mGFP tagged |
CNY 5,890.00 |
|
| RG205927 | DNASE1 (tGFP-tagged) - Human deoxyribonuclease I (DNASE1) |
CNY 5,200.00 |
|
| SC324542 | DNASE1 (untagged)-Human deoxyribonuclease I (DNASE1) |
CNY 3,600.00 |
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