VAMP3 (NM_004781) Human Untagged Clone

CAT#: SC117174

VAMP3 (untagged)-Human vesicle-associated membrane protein 3 (cellubrevin) (VAMP3)



  "NM_004781" in other vectors (6)

CNY 1,200.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
VAMP3/Cellubrevin Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CEB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>SC117174 representing NM_004781.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GAATTCGGAACGAGGTCCCAACTTCGCTTCTCTGCTGACCCTCTCTCGTCGCCGCTGCCGCCGCCGCAG
CTGCCAAAATGTCTACAGGTCCAACTGCTGCCACTGGCAGTAATCGAAGACTTCAGCAGACACAAAATC
AAGTAGATGAGGTGGTGGACATAATGCGAGTTAACGTGGACAAGGTTCTGGAAAGAGACCAGAAGCTCT
CTGAGTTAGACGACCGTGCAGACGCACTGCAGGCAGGCGCTTCTCAATTTGAAACGAGCGCAGCCAAGT
TGAAGAGGAAATATTGGTGGAAGAATTGCAAGATGTGGGCAATCGGGATTACTGTTCTGGTTATCTTCA
TCATCATCATCATCGTGTGGGTTGTCTCTTCATGAAGAACCAGCGGAACTCAAAACTGCTGTTCAAGAA
ACCTCTTCAAGACTTTTGACTTAGAACCTGCTATATTATCAAGCTTACCTACTGTTATCTCTAAAATTT
TTTTTGTGTTAATGTAAAGTTGAATTTCTAGGAAACGTGCCTTTGTTTTTTAATATGCACTCCAAATTA
GAAGGCCGGCCCCGTCCACATTTTGCACAGTGCCTTTACAGATTTACGTATGGGCTGATGAAGAGGCCT
TCTTAAGTTCCAGAGTGCTATAATCTAGATGTAATGTTGTCACTAATTAATTGCCATTACTCCCAGTTA
GTTACCCTTGTCATTTGGCATTATTTTCAGAACCACATTTTAAACCTTTGGGTAATCAGATTTCCAACT
TATGCCTTCCAGAAAAAAACACTACTGCCTAACACAAATCTGTGATAACAACAGGCTGTGCCTTATTTT
GATAATTTTCTGATTCCCTAGAAGAGAACCCTCTACTTTTTGTAAGCACTACTGACTCTCGCTGTATTT
AAGATGCTGGTGAAGAGCTTTTGCTCTTGCATTAGATTTGAAGATGTTTACATTGTTGTTATTGTTATG
TATCACTTGCTAAAAATATTGTTTTAATCAGAGATAACCTCTTTAAAAAAATTTTTAAAGAACTATGGC
TATGACCAAAGCTTCTATTTTGCCAAAAAGTTAAATACCGATAAAATGGCCTTAAGTGTATTCCTGACA
GTTAAATTCAGAAACGTGCCAAATGGAACTCAAGGTGCCCCTTCAGAATTAAAATCATTACCTTGTGTG
TGAACCTTCTACATCTTCATAGGCCTTTCTTCCTTTTGAAAGGCTGTAGACAGTGTGGCTCCCCTTCTG
ATTCAGTATTTTGCATGGGGGTTAGAGAAGGTTTGAGGTAGACTCTGACCGTCTCATAAAAGAGTTCTA
CCCAGCAGTTGGCAGATTATCAGCTGTGGACTCCAGCATGTTTCTGATAATTATGCAAGCAACAATTCT
GTAGCCTCAAGTAAGACCACCTGTGAACTTGATCATTATCTGGCCCAAATATGAAGATAAACTATAACT
TTGGAGTTTGTTTCCTATTTGTATTCACATTCTGCTTCCTAAATCAGTTTTCTAAATTATGCCTGCAAT
TAGGCATTGGTCAGGGGTGAATGGCTCTTTTCACAGAGAGTAGCCAACCAGAGACCTTTGCTTTGATAT
CATCAACTGCAGACAATGCTGTTGATGGGAATGCTGGAAGCAGAAACTTTGTCATCGGAAAAACTTTTC
TTGTATGCATGAGACTCAACATCAGGATCCACAGCTTAAAGATGGGAATTCAGGTATGAAAGAAAACAG
GCAAGGAGGCACTGAGGGAGAAAGACACAGACTTTATCGCTCTGTGGCTCATTGTTACTGGAATATTCT
AAAACTCTTGTTCACATGCTATTATGACTTATAAAGCAGCAACAGCTGAGGCGCACCAGGACACAGCTT
CCATTTCTTTAACGTCTGTTCCCTTAACATCGCTGAAATGATTTACTGTTGAAGAGATGCCTTGCGGTG
TGGCCAGCTGTGAGGAGAAAGCAGCTGGCAGTGTTAGGACATTAGTCCACCTTCAGCGCAGGGTCTCTG
GCCGGGTCTGACTCAGAAACCTTGGTACTCGCCCCTTGGCCACAGTGCCCAGACCCATGTAACCCACTG
GCTCCTGCATTAACCCAGAAATACCTCGCTTCTATCTGTGCACTTAGCTGGGAACTTACCCACTGTAAT
CACCTAAATAAAGTGTTTATAAACATAAAAAAAAAAAAAAAAAAAACTCGAC

>OriGene 5' read for NM_004781 unedited
GGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGNAACGAGTCCCAACTT
CGCTTCTCTGCTGACCCTCTCTCGTCGCCGCTGCCGCCGCCGCAGCTGCCAAAATGTCTA
CAGGTCCAACTGCTGCCACTGGCAGTAATCGAAGACTTCAGCAGACACAAAATCAAGTAG
ATGAGGTGGTGGACATAATGCGAGTTAACGTGGACAAGGTTCTGGAAAGAGACCAGAAGC
TCTCTGAGTTAGACGACCGTGCAGACGCACTGCAGGCAGGCGCTTCTCAATTTGAAACGA
GCGCAGCCAAGTTGAAGAGGAAATATTGGTGGAAGAATTGCAAGATGTGGGCAATCGGGA
TTACTGTTCTGGTTATCTTCATCATCATCATCATCGTGTGGGTTGTCTCTTCATGAAGAA
CCAGCGGAACTCAAAACTGCTGTTCAAGAAACCTCTTCAAGACTTTTGACTTAGAACCTG
CTATATTATCAAGCTTACCTACTGTTATCTCTAAAATTTTTTTTGTGTTAATGTAAAGTT
GAATTTCTAGGAAACGTGCCTTTGTTTTTTAATATGCACTCCAAATTAGAAGGCCGGCCC
CGTCCACATNTTGCACAGTGCCTTTACAGATTTACGTATGGGCTGATGAAGAGGCCCTTC
TTAAGTTCCAGAGTGCTATAATCCTAGATGTAATGTTGTCACTAATTAATTGCCATTACT
CCCAGTTAGTTACCCTTGTCATTTGGCATCATTTTCAAACCACATTTTTAACCTTTTGGG
TAATCAGAATTCCAACTTATGCCTTCCAAAAAAAAACCTACTGCCTAACACAAATCTGGG
ATAACACCAGCTGTGCCTTATTTGATAATTTTCTGATCCCCTAGAGAGACCCTCTACTTT
TGTAACACTATGA
>OriGene 3' read for NM_004781 unedited
GCTATGGACCGGCGGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTATGTTT
ATAAACACTTTATTTAGGTGATTACAGTGGGTAAGTTCCCAGCTAAGTGCACAGATAGAA
GCGAGGTATTTCTGGGTTAATGCAGGAGCCAGTGGGTTACATGGGTCTGGGCACTGTGGC
CAAGGGGCGAGTACCAAGGTTTCTGAGTCAGACCCGGCCAGAGACCCTGCGCTGAAGGTG
GACTAATGTCCTAACACTGCCAGCTGCTTTCTCCTCACAGCTGGCCACACCGCAAGGCAT
CTCTTCAACAGTAAATCATTTCAGCGATGTTAAGGGAACAGACGTTAAAGAAATGGAAGC
TGTGTCCTGGTGCGCCTCAGCTGTTGCTGCTTTATAAGTCATAATAGCATGTGAACAAGA
GTTTTAGAATATTCCAGTAACAATGAGCCACAGAGCGATAAAGTCTGTGTCTTTCTCCCT
CAGTGCCTCCTTGCCTGTTTTCTTTCATACCTGAATTCCCATCTTTAAGCTGTGGATCCT
GATGTTGAGTCTCATGCATACAAGAAAAGTTTTTCCGATGACAAAGTTTCTGCTTCCAGC
ATTCCCATCAACAGCATTGTCTGCAGTTGATGATATCAAAGCAAAGGTCTCTGGTTGGCT
ACTCTCTGTGAAAAGAGCCATTCACCCCTGACCAATGCCTAATTGCAGGCATAATTTAGA
AAACTGATTTAGGAAGCAGAATGTGAATACAAATAGGAAACAAACTCCAAAGTTATAGCT
TATCTTCATATTTGGGCCCAGATAATGATCAAGTTCACAGGTGGTCTTACTTGANGCTAC
AGAANTGTTGCTTGCATAATATCAGAAACATGCTGNAGTCCCAGCTGATATCTGCCACTG
CTGGGTAAACTCTTTATGAGACGTCAGAGCTACTAAACCTTCCTAACCCCAGCA
Restriction Sites NotI-NotI     
ACCN NM_004781
Insert Size 2191 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004781.3
RefSeq Size 2225 bp
RefSeq ORF 2191 bp
Locus ID 9341
UniProt ID Q15836
Domains synaptobrevin
Protein Families Transmembrane
Protein Pathways SNARE interactions in vesicular transport
MW 84.1 kDa
Gene Summary Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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