YB1 (YBX1) (NM_004559) Human Untagged Clone
CAT#: SC117278
YBX1 (untagged)-Human Y box binding protein 1 (YBX1)
CNY 2,400.00
CNY 2,950.00
Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BP-8; CBF-A; CSDA2; CSDB; DBPB; EFI-A; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC117278 sequence for NM_004559 edited (data generated by NextGen Sequencing)
ATGAGCAGCGAGGCCGAGACCCAGCAGCCGCCCGCCGCCCCCCCCGCCGCCCCCGCCCTC AGCGCCGCCGACACCAAGCCCGGCACTACGGGCAGCGGCGCAGGGAGCGGTGGCCCGGGC GGCCTCACATCGGCGGCGCCTGCCGGCGGGGACAAGAAGGTCATCGCAACGAAGGTTTTG GGAACAGTAAAATGGTTCAATGTAAGGAACGGATATGGTTTCATCAACAGGAATGACACC AAGGAAGATGTATTTGTACACCAGACTGCCATAAAGAAGAATAACCCCAGGAAGTACCTT CGCAGTGTAGGAGATGGAGAGACTGTGGAGTTTGATGTTGTTGAAGGAGAAAAGGGTGCG GAGGCAGCAAATGTTACAGGTCCTGGTGGTGTTCCAGTTCAAGGCAGTAAATATGCAGCA GACCGTAACCATTATAGACGCTATCCACGTCGTAGGGGTCCTCCACGCAATTACCAGCAA AATTACCAGAATAGTGAGAGTGGGGAAAAGAACGAGGGATCGGAGAGTGCTCCCGAAGGC CAGGCCCAACAACGCCGGCCCTACCGCAGGCGAAGGTTCCCACCTTACTACATGCGGAGA CCCTATGGGCGTCGACCACAGTATTCCAACCCTCCTGTGCAGGGAGAAGTGATGGAGGGT GCTGACAACCAGGGTGCAGGAGAACAAGGTAGACCAGTGAGGCAGAATATGTATCGGGGA TATAGACCACGATTCCGCAGGGGCCCTCCTCGCCAAAGACAGCCTAGAGAGGACGGCAAT GAAGAAGATAAAGAAAATCAAGGAGATGAGACCCAAGGTCAGCAGCCACCTCAACGTCGG TACCGCCGCAACTTCAATTACCGACGCAGACGCCCAGAAAACCCTAAACCACAAGATGGC AAAGAGACAAAAGCAGCCGATCCACCAGCTGAGAATTCGTCCGCTCCCGAGGCTGAGCAG GGCGGGGCTGAGTAA Clone variation with respect to NM_004559.3 >OriGene 5' read for NM_004559 unedited
TGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGGGAGCGGAGAGCGGACCC CAGAGAGCCCTGAGCAGCCCCACCGCCGCCGCCGGCCTAGTTACCATCACACCCCGGGAG GAGCCGCAGCTGCCGCAGCCGGCCCCAGTCACCATCACCGCAACCATGAGCAGCGAGGCC GAGACCCAGCAGCCGCCCGCCGCCCCCCCCGCCGCCCCCGCCCTCAGCGCCGCCGACACC AAGCCCGGCACTACGGGCAGCGGCGCAGGGAGCGGTGGCCCGGGCGGCCTCACATCGGCG GCGCCTGCCGGCGGGGACAAGAAGGTCATCGCAACGAAGGTTTTGGGAACAGTAAAATGG TTCAATGTAAGGAACGGATATGGTTTCATCAACAGGAATGACACCAAGGAAGATGTATTT GTACACCAGACTGCCATAAAGAAGAATAACCCCAGGAAGTACCTTCGCAGTGTAGGAGAT GGAGAGACTGTGGAGTTTGATGTTGTTGAAGGAGAAAAGGGTGCGGAGGCAGCAAATGTT ACAGGTCCTGGTGGTGTTCCAGTTCAAGGCAGTAAATATGCAGCAGACCGTAACCATTAT AGACGCTATCCACGTCGTNAGGGGGTCCTCCACGCAATTACCAGCAAAATTACCAGAATA GTGGAGAGTGGGGGAAAAGAACGAGGGATCGGNAGAGTGCTNNCCGAAGGCCAGGCCCAA CAACGCCGGCCCCTACCGCAGGCGAAAGGTCCCACCTTACTACATGCGGAGACCCTAATG GGCGTCGACCACAGTATTCCAACCCTCTGTGCAGGGAGAGGATTGGGAGGGTGCTGACAC CCAGGTGCAGGAGAACAAGGTAGACCAGTGAGCAGATATGTATTCGGGGAATAGACACGA TNCCGCAGG >OriGene 3' read for NM_004559 unedited
CACAACACAAAACCCCACAAAACAACCCCCCCCCCACAAACCCATTGCTTTTTACTCTGC NCNCGACGGCCCGCTTTTTNANGATCGAGTTTTTTTTTTTTTTTTTTTAATTATTTAAGA CCTTTATTAACAGGTGCTTGCAGTTTGTTGACTTTTTTCGAAAAAATCAAGTTGTAAACT TTTATTACAAATTAAAAATGAAGTTCTTAAAAATCTCAACTTGACCAGATATGAAACAAT TTAAAAACCTTTAAAGGCGTATTGAGAAAAACCAGGCTTTTTTAAAAAACACGTTTGTTA TTACCAAAAAGAGACGTCTTTAGGTAAAAATAATAAAAACCCCATGCTGCATAGATACTG CAGATAGTTCTATTTATCTGGTCAACGGGCAAAAAGCAAGCACTTTAGGTCTTCAGCTCC AATCTTTTGTTCATTTCTTATTGCTGGAATTTCATATTTCTTCTTGTTGGATGACTAAAC CGGATGATGGTAGAGATGGTAAGCCGGCATTTACTCAGCCCCGCCCTGCTCAGCCTCGGG AGCGGACGAATTCTCAGCTGGTGGATCGGCTGCCTTTGTCTCTTTGCCATCTTGTGGTTT AGGGTCTTCTGGGCGTCTGCGTCGGTAATTGAAGTTGCGGCGGTACCGACGTTGAGGTGG CTGTTGACCTTGGGCCTCATCTCCTTGATTTTCTTTATCTTCTTAATTGCCCGCCTCTCT AGGCTTGCTTTGGCGAGGAGGGCCCCTGGGGAACCGGGGCTATACCCCCCAACCTATCCT GCCTCCTGGGTTACCTTGCTCCCCTGCACCCCGGTTGGCAGCACCCTCCATAATTTTTCC CTGCCCAGGAGGGTTTGGAATCCTGTGCCCCACCCCCTAAGGTCTTCCCATGGCACAAGG GGGGAACCCTTCCCCTGCGGGAGGG |
Restriction Sites | NotI-NotI |
ACCN | NM_004559 |
Insert Size | 1560 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004559.2, NP_004550.2 |
RefSeq Size | 1554 bp |
RefSeq ORF | 975 bp |
Locus ID | 4904 |
UniProt ID | P67809 |
Domains | CSD |
Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
Gene Summary | This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Extracellular signal-regulated kinase (ERK)-dependent phosphorylation of Y-Box binding protein 1 (YB-1) enhances gene expression in granulosa cells in response to follicle stimulating hormone (FSH)
,Donaubauer, EM;Hunzicker-Dunn, ME;,
J. Biol. Chem.
,PubMed ID 27080258
[YBX1]
|
Y-Box-Binding Protein 1 Interacts with Hepatitis C Virus NS3/4A and Influences the Equilibrium between Viral RNA Replication and Infectious Particle Production
,Laurent Chatel-Chaix, Pierre Melançon, Marie-Ève Racine, Martin Baril, and Daniel Lamarre,
J. Virol., Nov 2011; 85: 11022 - 11037.
[YBX1]
|
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