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Glypican 3 (GPC3) (NM_004484) Human Untagged Clone
CAT＃: SC117333
GPC3 (untagged)-Human glypican 3 (GPC3), transcript variant 2
CNY 6,488.00
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DGSX; GTR2-2; MXR7; OCI-5; SDYS; SGB; SGBS; SGBS1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117333 sequence for NM_004484 edited (data generated by NextGen Sequencing)
ATGGCCGGGACCGTGCGCACCGCGTGCTTGGTGGTGGCGATGCTGCTCAGCTTGGACTTC CCGGGACAGGCGCAGCCCCCGCCGCCGCCGCCGGACGCCACCTGTCACCAAGTCCGCTCC TTCTTCCAGAGACTGCAGCCCGGACTCAAGTGGGTGCCAGAAACTCCCGTGCCAGGATCA GATTTGCAAGTATGTCTCCCTAAGGGCCCAACATGCTGCTCAAGAAAGATGGAAGAAAAA TACCAACTAACAGCACGATTGAACATGGAACAGCTGCTTCAGTCTGCAAGTATGGAGCTC AAGTTCTTAATTATTCAGAATGCTGCGGTTTTCCAAGAGGCCTTTGAAATTGTTGTTCGC CATGCCAAGAACTACACCAATGCCATGTTCAAGAACAACTACCCAAGCCTGACTCCACAA GCTTTTGAGTTTGTGGGTGAATTTTTCACAGATGTGTCTCTCTACATCTTGGGTTCTGAC ATCAATGTAGATGACATGGTCAATGAATTGTTTGACAGCCTGTTTCCAGTCATCTATACC CAGCTAATGAACCCAGGCCTGCCTGATTCAGCCTTGGACATCAATGAGTGCCTCCGAGGA GCAAGACGTGACCTGAAAGTATTTGGGAATTTCCCCAAGCTTATTATGACCCAGGTTTCC AAGTCACTGCAAGTCACTAGGATCTTCCTTCAGGCTCTGAATCTTGGAATTGAAGTGATC AACACAACTGATCACCTGAAGTTCAGTAAGGACTGTGGCCGAATGCTCACCAGAATGTGG TACTGCTCTTACTGCCAGGGACTGATGATGGTTAAACCCTGTGGCGGTTACTGCAATGTG GTCATGCAAGGCTGTATGGCAGGTGTGGTGGAGATTGACAAGTACTGGAGAGAATACATT CTGTCCCTTGAAGAACTTGTGAATGGCATGTACAGAATCTATGACATGGAGAACGTACTG CTTGGTCTCTTTTCAACAATCCATGATTCTATCCAGTATGTCCAGAAGAATGCAGGAAAG CTGACCACCACTATTGGCAAGTTATGTGCCCATTCTCAACAACGCCAATATAGATCTGCT TATTATCCTGAAGATCTCTTTATTGACAAGAAAGTATTAAAAGTTGCTCATGTAGAACAT GAAGAAACCTTATCCAGCCGAAGAAGGGAACTAATTCAGAAGTTGAAGTCTTTCATCAGC TTCTATAGTGCTTTGCCTGGCTACATCTGCAGCCATAGCCCTGTGGCGGAAAACGACACC CTTTGCTGGAATGGACAAGAACTCGTGGAGAGATACAGCCAAAAGGCAGCAAGGAATGGA ATGAAAAACCAGTTCAATCTCCATGAGCTGAAAATGAAGGGCCCTGAGCCAGTGGTCAGT CAAATTATTGACAAACTGAAGCACATTAACCAGCTCCTGAGAACCATGTCTATGCCCAAA GGTAGAGTTCTGGATAAAAACCTGGATGAGGAAGGGTTTGAAAGTGGAGACTGCGGTGAT GATGAAGATGAGTGCATTGGAGGCTCTGGTGATGGAATGATAAAAGTGAAGAATCAGCTC CGCTTCCTTGCAGAACTGGCCTATGATCTGGATGTGGATGATGCGCCTGGAAACAGTCAG CAGGCAACTCCGAAGGACAACGAGATAAGCACCTTTCACAACCTCGGGAACGTTCATTCC CCGCTGAAGCTTCTCACCAGCATGGCCATCTCGGTGGTGTGCTTCTTCTTCCTGGTGCAC TGA Clone variation with respect to NM_004484.3 >OriGene 5' read for NM_004484 unedited
GAGATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGCGCCCAGGTAGCT GCGAGGAAACTTTTGCAGCGGCTGGGTAGCAGCACGTCTCTTGCTCCTCAGGGCCACTGC CAGGCTTGCCGAGTCCTGGGACTGCTCTCGCTCCGGCTGCCACTCTCCCGCGCTCTCCTA GCTCCCTGCGAAGCAGGATGGCCGGGACCGTGCGCACCGCGTGCTTGGTGGTGGCGATGC TGCTCAGCTTGGACTTCCCGGGACAGGCGCAGCCCCCGCCGCCGCCGCCGGACGCCACCT GTCACCAAGTCCGCTCCTTCTTCCAGAGACTGCAGCCCGGACTCAAGTGGGTGCCAGAAA CTCCCGTGCCAGGATCAGATTTGCAAGTATGTCTCCCTAAGGGCCCAACATGCTGCTCAA GAAAGATGGAAGAAAAATACCAACTAACAGCACGATTGAACATGGAACAGCTGCTTCAGT CTGCAAGTATGGAGCTCAAGTTCTTAATTATTCAGAATGCTGCGGTTTTCCAAGAGGCCT TTGAAATTGTTGTTCGCCATGCCAAGAACTACACCAATGCCATGTTCAAGAACAACTACC CAAGCCTGACTCCACAAGCTTTTGAGTTTGTGGGTGAATTTTTCACAGATGTGTCTCTCT ACCATCTGGGTTCTGACATCAATGTAGATGACATGCTCAATGAATTGTTTGACAGCCTGT TTCCAGTCATCTATACCCAGCTAATGAACCCCAGCCCTGCCTGATTCACCCTTGGACATC AATGAGTGCCTCCCGAGGAGCAAGACGTGACCCTGAAAGTATTTGGGAATTTCCCCAGCT TATTATGACCCAGNTCTCCAAGCCCTGGAAGTCACTAGACCTTCCTTAGGCTCTGATCCT GGATGGAGGGTCACCCAACTGACCCCTGAGTTCATAGGACTGGGCC >OriGene 3' read for NM_004484 unedited
GCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAGCAATTTATTTTTTTTCA AAGAAATCCATGCAAAGAGAGAACGATAGAAAGGGAGGGAGTGAGAGAGAAAGACATGGT AACCATGTTCTAGCAGCCAAACATAGGAGAATAATTTGGCACAACTTGATGGTTTTTTTT CTTTCTTTGCAAAAGGACAATCTATATGCTACCACTAAAATGTATCTTCCTCCAAAAGAT ACCATTTGATTTTCGAAAACATAACACATGGTTAGTCCTCTACTTCATGGCTGGAGGAGG TATACAGGATAACAAAAAAAAAAAAATAGAAAAAAATAAGAAAGTGGTTCCCTTTATCGA GGAAGACCACAGGGTGCTGTAGGGCAGCACATGTGCTGGGCACCAGGCAGTCAGTGCACC AGGAAGAAGAAGCACACCACCGAGATGGCCATGCTGGTGAGAAGCTTCAGCGGGGAATGA ACGTTCCCGAGGTTGTGAAAGGTGCTTATCTCGTTGTCCTTCGGAGTTGCCTGCTGACTG TTTCCAGGCGCATCATCCACATCCAGATCATAGGCCAGTTCTGCAAGGAAGCGGAGCTGA TTCTTCACTTTTATCATTCCATCACCAGAGCCTNCAATGCACTCATCTTCATCATCACCG CAGTCTCCACTTTCAAACCCTTCCTCATCCAGGTTTTTATCCAGAACTCTACCTTTTGGC ATAGACATGGCTCTCAGGAGCTGTNTATGTGCCTCAGTTTGTCAATAATTGACTGACACT GCCTCAGGCCCCTTCATTTCAGCTCATGGAGATGGAACGGGTTTTCATCCAATCCTTGCT GCCTTTGGCTGGACTCTCCCGAGTTTCTGTCCTTTCAGCCAAGGTGGCGTTTCCGCCACA GGCTATGCTGCGAAGTACCCCGCAAGACTTTAAAGCTGGGAAGACTCACCTCTGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004484 |
| Insert Size | 2550 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004484.2, NP_004475.1 |
| RefSeq Size | 2382 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 2719 |
| UniProt ID | P51654 |
| Domains | Glypican |
| Protein Families | Druggable Genome |
| Gene Summary | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205911 | GPC3 (Myc-DDK-tagged)-Human glypican 3 (GPC3), transcript variant 2 |
CNY 4,320.00 |
|
| RC205911L1 | Lenti-ORF clone of GPC3 (Myc-DDK-tagged)-Human glypican 3 (GPC3), transcript variant 2 |
CNY 6,720.00 |
|
| RC205911L2 | Lenti-ORF clone of GPC3 (mGFP-tagged)-Human glypican 3 (GPC3), transcript variant 2 |
CNY 5,890.00 |
|
| RC205911L3 | Lenti-ORF clone of GPC3 (Myc-DDK-tagged)-Human glypican 3 (GPC3), transcript variant 2 |
CNY 6,720.00 |
|
| RC205911L4 | Lenti-ORF clone of GPC3 (mGFP-tagged)-Human glypican 3 (GPC3), transcript variant 2 |
CNY 5,890.00 |
|
| RG205911 | GPC3 (tGFP-tagged) - Human glypican 3 (GPC3), transcript variant 2 |
CNY 5,920.00 |
|
| SC324622 | GPC3 (untagged)-Human glypican 3 (GPC3), transcript variant 2 |
CNY 6,488.00 |
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