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Dystrophia myotonica protein kinase (DMPK) (NM_004409) Human Untagged Clone
CAT＃: SC117404
DMPK (untagged)-Human dystrophia myotonica-protein kinase (DMPK), transcript variant 2
CNY 4,688.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DM; DM1; DM1PK; DMK; MDPK; MT-PK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_004409 edited
ATGTCAGCCGAGGTGCGGCTGAGGCGGCTCCAGCAGCTGGTGTTGGACCCGGGCTTCCTG GGGCTGGAGCCCCTGCTCGACCTTCTCCTGGGCGTCCACCAGGAGCTGGGCGCCTCCGAA CTGGCCCAGGACAAGTACGTGGCCGACTTCTTGCAGTGGGCGGAGCCCATCGTGGTGAGG CTTAAGGAGGTCCGACTGCAGAGGGACGACTTCGAGATTCTGAAGGTGATCGGACGCGGG GCGTTCAGCGAGGTAGCGGTAGTGAAGATGAAGCAGACGGGCCAGGTGTATGCCATGAAG ATCATGAACAAGTGGGACATGCTGAAGAGGGGCGAGGTGTCGTGCTTCCGTGAGGAGAGG GACGTGTTGGTGAATGGGGACCGGCGGTGGATCACGCAGCTGCACTTCGCCTTCCAGGAT GAGAACTACCTGTACCTGGTCATGGAGTATTACGTGGGCGGGGACCTGCTGACACTGCTG AGCAAGTTTGGGGAGCGGATTCCGGCCGAGATGGCGCGCTTCTACCTGGCGGAGATTGTC ATGGCCATAGACTCGGTGCACCGGCTTGGCTACGTGCACAGGGACATCAAACCCGACAAC ATCCTGCTGGACCGCTGTGGCCACATCCGCCTGGCCGACTTCGGCTCTTGCCTCAAGCTG CGGGCAGATGGAACGGTGCGGTCGCTGGTGGCTGTGGGCACCCCAGACTACCTGTCCCCC GAGATCCTGCAGGCTGTGGGCGGTGGGCCTGGGACAGGCAGCTACGGGCCCGAGTGTGAC TGGTGGGCGCTGGGTGTATTCGCCTATGAAATGTTCTATGGGCAGACGCCCTTCTACGCG GATTCCACGGCGGAGACCTATGGCAAGATCGTCCACTACAAGGAGCACCTCTCTCTGCCG CTGGTGGACGAAGGGGTCCCTGAGGAGGCTCGAGACTTCATTCAGCGGTTGCTGTGTCCC CCGGAGACACGGCTGGGCCGGGGTGGAGCAGGCGACTTCCGGACACATCCCTTCTTCTTT GGCCTCGACTGGGATGGTCTCCGGGACAGCGTGCCCCCCTTTACACCGGATTTCGAAGGT GCCACCGACACATGCAACTTCGACTTGGTGGAGGACGGGCTCACTGCCATGGTGAGCGGG GGCGGGGAGACACTGTCGGACATTCGGGAAGGTGCGCCGCTAGGGGTCCACCTGCCTTTT GTGGGCTACTCCTACTCCTGCATGGCCCTCAGGGACAGTGAGGTCCCAGGCCCCACACCC ATGGAACTGGAGGCCGAGCAGCTGCTTGAGCCACACGTGCAAGCGCCCAGCCTGGAGCCC TCGGTGTCCCCACAGGATGAAACAGCTGAAGTGGCAGTTCCAGCGGCTGTCCCTGCGGCA GAGGCTGAGGCCGAGGTGACGCTGCGGGAGCTCCAGGAAGCCCTGGAGGAGGAGGTGCTC ACCCGGCAGAGCCTGAGCCGGGAGATGGAGGCCATCCGCACGGACAACCAGAACTTCGCC AGTCAACTACGCGAGGCAGAGGCTCGGAACCGGGACCTAGAGGCACACGTCCGGCAGTTG CAGGAGCGGATGGAGTTGCTGCAGGCAGAGGGAGCCACAGCTGTCACGGGGGTCCCCAGT CCCCGGGCCACGGATCCACCTTCCCATATGGCCCCCCGGCCGTGGCTGTGGGCCAGTGCC CGCTGGTGGGGCCAGGCCCCATGCACCGCCGCCACCTGCTGCTCCCTGCCAGGGTCCCTA GGCCTGGCCTATCGGAGGCGCTTTCCCTGCTCCTGTTCGCCGTTGTTCTGTCTCGTGCCG CCGCCCTGGGCTGCATTGGGTTGGTGGCCCACGCCGGCCAACTCACCGCAGTCTGGCGCC GCCCAGGAGCCGCCCGCGCTCCCTGA >OriGene 5' read for NM_004409 unedited
CAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGACAGCCA GGGACAGGCAGACATGCAGCCAGGGCTCCAGGGCCTGGACAGGGGCTGCCAGGCCCTGTG ACAGGAGGACCCCGAGCCCCCGGCCCGGGGAGGGGCCATGGTGCTGCCTGTCCAACATGT CAGCCGAGGTGCGGCTGAGGCGGCTCCAGCAGCTGGTGTTGGACCCGGGCTTCCTGGGGC TGGAGCCCCTGCTCGACCTTCTCCTGGGCGTCCACCAGGAGCTGGGCGCCTCCGAACTGG CCCAGGACAAGTACGTGGCCGACTTCTTGCAGTGGGCGGAGCCCATCGTGGTGAGGCTTA AGGAGGTCCGACTGCAGAGGGACGACTTCGAGATTCTGAAGGTGATCGGACGCGGGGCGT TCAGCGAGGTAGCGGTAGTGAAGATGAAGCAGACGGGCCAGGTGTATGCCATGAAGATCA TGAACAAGTGGGACATGCTGAAGAGGGGCGAGGTGTCGTGCTTCCGTGAGGAGAGGGACG TGTTGGTGAATGGGGACCGGCGGTGGATCACGCAGCTGCACTTCGCCTTCCAGGATGAGA ACTACCTGTACCTGGTCATGGAGTATTACGTGGGCGGNGACCTGCTGACACTGCTGAGCA AGTTTGGGGAGCGGATTCCGGCCGAGATGGCGCGCTTCTACCTGGCGGAGATTGTCATGG CCATAGACTCGGTGCACCGGCTTGGCTACGTGCACAGGGACATCAAACCCGACACATNCT GCTGGACCGCTGTGGCCACATNCGNCTGGCCGACTTCNGCTCTTGCCTCAAGCTGCNGGC AGATGGAACCNGTGCNGTCGCTGGNTGGCTGTGGGCACCCCANACTACCCTGTCCCCGAG ATCCTGCAGCTGTGGNNCNGTGGGCCCTGGACANNGCACTACGGNCCCNNATGTGACTGG TGGCGCCTGGGNTGTATCGCCTATGAATGTT >OriGene 3' read for NM_004409 unedited
TATCTATGNNACCGCGGCCGCAATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTG GGCAGAAGGAGGGCCTTTTATTCGCGAGGGTCGGGGGTGGGGGTCCTAGGTGGGGACAGA CAATAAATACCGAGGAATGTCGGGGTCTCAGTGCATCCAAAACGTGGATTGGGGTTGTTG GGGGTCCTGTAGCCTGTCAGCGAGTCGGAGGACGAGGTCAATAAATATCCAAACCGCCGA AGCGGGCGGAGCCGGCTGGGGCTCCGAGAGCAGCATAAGTGAGGAGGGGGGCGCGGGATC CCCGAAAAAGCGGGTTTGGCAAAAGCAAATTTCCCGAGTAAGCAGGCAGAGATCGCGCCA GACGCTCCCCAGAGCAGGGCGTCATGCACAAGAAAGCTTTGCACTTTGCGAACCAACCAT AGGTGGGGGTGCGTGGAGGATGGAACACGGACGGCCCGGCTTGCTGCCTTCCCAGGCCTG CAGTTTGCCCATCCACGTCAGGGCCTCAGCCTGGCCGAAAGAAAGAAATGGTCTGTGATC CCCCCAGCAGCAGCAGCAGCATTCCCGGCTACAAGGACCCTTCGAGCCCCGTTCGCCGGC CGCGGACCCGGCCCCTCCCTCCCCGGCCGCTAGGGGGCGGGCCCGGATCACAGGACTGGA GCTGGGCGGAGACCCACGCTCGGAGCGGTTGTGAACTGGCAGGCGGTGGGCGCGGCTTCT GTGCCGTGCCCCGGGCACTCAGTCTCCAACGGGGCCCCCGAGTCGAAGAAGTTCTAGGGT TCAAGGAACGCGGGCGGCTCCTGGGCGGCGCCAGACTGCGGTGAGTTTCCCCGCGTGGGC CACACACCAATGGAGCCCAAGCGGCGGGCCGAGAAAAACAACGGGGAACAGGAGCAAGGA AAGCCCCTCGATAGCCAGGCTTAGGACCCTGCAGGGAGCAACAGGTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004409 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004409.2, NP_004400.4 |
| RefSeq Size | 2768 bp |
| RefSeq ORF | 1890 bp |
| Locus ID | 1760 |
| UniProt ID | Q09013 |
| Domains | pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-38 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR and cause translation initiation at an alternative start codon, compared to variant 1. The encoded protein (isoform 2, also known as isoform 9) has a distinct N-terminus and is shorter than isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209151 | DMPK (Myc-DDK-tagged)-Human dystrophia myotonica-protein kinase (DMPK), transcript variant 2 |
CNY 4,680.00 |
|
| RC209151L1 | Lenti ORF clone of Human dystrophia myotonica-protein kinase (DMPK), transcript variant 2, Myc-DDK-tagged |
CNY 7,080.00 |
|
| RC209151L2 | Lenti ORF clone of Human dystrophia myotonica-protein kinase (DMPK), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC209151L3 | Lenti ORF clone of Human dystrophia myotonica-protein kinase (DMPK), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209151L4 | Lenti ORF clone of Human dystrophia myotonica-protein kinase (DMPK), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG209151 | DMPK (tGFP-tagged) - Human dystrophia myotonica-protein kinase (DMPK), transcript variant 2 |
CNY 6,280.00 |
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