APPBP1 (NAE1) (NM_003905) Human Untagged Clone

CAT#: SC117674

NAE1 (untagged)-Human NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1



  "NM_003905" in other vectors (6)

CNY 3,984.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Purified NAE1 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms A-116A10.1; APPBP1; HPP1; ula-1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117674 sequence for NM_003905 edited (data generated by NextGen Sequencing)
ATGGCGCAGCTGGGAAAGCTGCTCAAGGAGCAGAAGTACGACCGGCAGCTGAGGTTGTGG
GGTGATCATGGGCAAGAGGCTTTAGAATCTGCTCATGTTTGCCTAATAAATGCAACAGCC
ACAGGAACTGAAATTCTTAAAAACTTGGTACTACCAGGTATTGGTTCGTTTACAATTATT
GATGGAAATCAGGTCAGCGGAGAAGATGCTGGAAACAATTTCTTCCTTCAAAGAAGCAGT
ATCGGCAAGAACCGAGCTGAAGCTGCCATGGAATTCTTACAAGAATTAAATAGCGATGTC
TCTGGAAGTTTTGTGGAAGAGAGTCCAGAAAACCTTCTAGACAATGATCCCTCATTTTTC
TGTAGGTTTACTGTTGTAGTTGCAACTCAGCTTCCTGAAAGCACTTCACTACGCTTAGCA
GATGTCCTCTGGAATTCCCAGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGT
TATATGAGGATCATTATAAAAGAACATCCAGTAATAGAATCTCATCCAGATAATGCATTA
GAGGATCTACGACTAGATAAGCCATTTCCTGAACTGAGAGAACATTTTCAGTCCTATGAT
TTGGATCATATGGAAAAAAAGGACCACAGTCATACTCCATGGATTGTGATCATAGCTAAA
TATTTAGCACAGTGGTATAGTGAAACAAATGGACGAATACCTAAAACGTATAAAGAAAAA
GAGGACTTCAGAGATTTGATTAGACAAGGAATTCTAAAAAATGAAAATGGGGCTCCAGAA
GATGAAGAGAATTTTGAAGAAGCTATTAAAAATGTGAACACAGCACTAAATACAACTCAG
ATCCCAAGCAGTATTGAAGATATATTTAATGATGATCGCTGCATAAATATCACCAAACAG
ACTCCATCATTTTGGATTTTAGCTCGTGCCTTAAAGGAATTTGTGGCCAAAGAGGGTCAA
GGAAATTTACCTGTTCGAGGCACAATTCCTGATATGATTGCAGATTCAGGCAAATATATA
AAACTGCAAAACGTTTACCGTGAAAAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCAT
GTTGCCAAATTGCTGCAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGAGAAAGAATTA
AAATTACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAAGATGTCGATCCTTAGCTGAA
GAATATGGTTTGGATACAATTAACAAGGATGAAATTATTTCTAGCATGGACAATCCAGAT
AATGAAATAGTGTTGTACTTAATGTTACGGGCTGTTGATAGATTTCATAAACAACAGGGT
AGATATCCAGGAGTATCTAACTATCAAGTTGAAGAAGATATAGGAAAGTTGAAGTCTTGT
CTCACTGGCTTCCTTCAGGAATATGGTTTATCTGTAATGGTGAAAGATGATTATGTCCAC
GAATTTTGCCGATATGGAGCTGCTGAGCCACATACCATTGCTGCATTCTTGGGGGGAGCT
GCTGCTCAAGAGGTCATCAAAATAATCACCAAACAATTTGTAATTTTTAATAATACTTAC
ATTTACAGTGGCATGTCACAAACTTCAGCAACTTTCCAGTTGTAG

Clone variation with respect to NM_003905.3
>OriGene 5' read for NM_003905 unedited
TGCGNCATATTTGGAATACCCATTCACTCATAGGGCGCGCCGCGCAATTCGGCACGAGGG
CTCCGCAGGGCTCGGCTGTTTTCCGCGCGGCAGGCGCGGCCATGGCGCACCTGGGAAAGC
TGCTCAAGGAGCAGAAGTACGACCGGCAGCTGAGGTTGTGGGGTGATCATGGGCAAGAGG
CTTTAGAATCTGCTCATGTTTGCCTAATAAATGCAACTTTTCCAGGAACTGAAATTCTTA
AAAACTTGGGACTACCAGGTATTGGCTCGTTTACAATTATTGATGGAAATCAGGTCAGCG
GAGAAGATGCTGGAAACAATTTCTTCCTTCAAAGAAGCAGTATCGGCAAGAACCGAGCTG
AAGCTGCCATGGAATTCTTACAAGAATTAAATAGCGATGTCTCTGGAAGTTTTGTGGAAG
AGAGTCCAGAAAACCTTCTAGACAATGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAG
TTGCAACTCAGCTTCCTGAAAGCACTTCACTACGCTTAGCAGATGTCCTCTGGAATTCCC
AGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGTTATATGAGGATCATTATAA
AAGAACATCCAGTAATAGAATCTCATCCAGATAATGCATTAGAGGATCTACGACTAGATA
AGCCATTTCCTGAACTGAGAGAACATTTTCAGTCCTATGATTGGGATCATATGGAAAAAA
AGGACCACAGTCATACTCCATGGATTGTGATCATAGCTAAATATTTAGCACAGTGGTATA
GTGAAACAAATGGACGAATTCCTTAAACGTACTAAGAACAAGAGGACTTCCCAGATTTGA
TTAGACAAGGAACTCCTAAAAATGAAAACGGGGCTCCAGAGAATGAAAAGAATTTTGAAA
AACTATTAAAAATGGGAACCCAGCCCTAAATACAACTCAN
>OriGene 3' read for NM_003905 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAAATGAGAAAAATGTTTATTAA
GAAAACAATTTAGCAGCTCTCCTTTAGAATTTTACAGACTAAAGCACAACCCGAAGGCAA
TTACAGTTTCAATCATTAACACACTACTTAAGGTGCTTGCTTACTCTACAACTGGAAAGT
TGCTGAAGTTTGTGACATGCCACTGTAAATGTAAGTATTATTAAAAATTACAAATTGTTT
GGTGATTATTTTGATGACCTCTTGAGCAGCAGCTCCCCCCAAGAATGCAGCAATGGTATG
TGGCTCAGCAGCTCCATATCGGCAAAATTCGTGGACATAATCATCTTTCACCATTACAGA
TAAACCATATTCCTGAAGGAAGCCAGTGAGACAAGACTTCAACTTTCCTATATCTTCTTC
AACTTGATAGTTAGATACTCCTGGATATCTACCCTGTTGTTTATGAAATCTATCAACAGC
CCGTAACATTAAGTACAACACTATTTCATTATCTGGATTGTCCATGCTAGAAATAATTTC
ATCCTTGTTAATTGTATCCAAACCATATTCTTCAGCTAAGGATCGACATCTTACCACTCG
AAGAAATGCAGAATTGCTGCAGAGTAATTNTAATTCTTTCTCTGAAATGGACTCTGGTGC
CTGGCCAATGGACTGCAGCAATTTGGCAACATGATTACCCACAGCGGCAGCATCTTTCTT
TGCTTTTTACGGTAAACGTTNTGCAGTTTTATATATTTGCCTGAATCTGNCATCATATCA
NGAATTGTGCCTCNGACAGGTAAAATTCCCTTGACCCTCTTNTGCCCNNAATCCTTTANG
CACGAGCTAAAATCCAATGATGGAGTCTGTTTGGTGATATTATGCAGCGATCATCATTAA
TATATCTCATACTGNCTGGNATCTGAGTTGNATTTAAGGCTGNGTTCACATTTTAATAGC
TCTTCAAAATCTCTCTCTNTCTGGACCCCATTTCATTNTTAGATNCCTGNCTATCAATCC
CT
Restriction Sites NotI-NotI     
ACCN NM_003905
Insert Size 1950 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003905.3, NP_003896.1
RefSeq Size 1820 bp
RefSeq ORF 1605 bp
Locus ID 8883
UniProt ID Q13564
Domains ThiF
Protein Pathways Alzheimer's disease
Gene Summary The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) encodes the predominant isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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