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Ceramide glucosyltransferase (UGCG) (NM_003358) Human Untagged Clone
CAT＃: SC118052
UGCG (untagged)-Human UDP-glucose ceramide glucosyltransferase (UGCG)
CNY 5,488.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GCS; GLCT1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118052 sequence for NM_003358 edited (data generated by NextGen Sequencing)
ATGGCGCTGCTGGACCTGGCCTTGGAGGGAATGGCCGTCTTCGGGTTCGTCCTCTTCTTG GTGCTGTGGCTGATGCATTTCATGGCTATCATCTACACCCGATTACACCTCAACAAGAAG GCAACTGACAAACAGCCTTATAGCAAGCTCCCAGGTGTCTCTCTTCTGAAACCACTGAAA GGGGTAGATCCTAACTTAATCAACAACCTGGAAACATTCTTTGAATTGGATTATCCCAAA TATGAAGTGCTCCTTTGTGTACAAGATCATGATGATCCAGCCATTGATGTATGTAAGAAG CTTCTTGGAAAATATCCAAATGTTGATGCTAGATTGTTTATAGGTGGCAAAAAAGTTGGC ATTAATCCTAAAATTAATAATTTAATGCCAGGATATGAAGTTGCAAAGTATGATCTTATA TGGATTTGTGATAGTGGAATAAGAGTAATTCCAGATACGCTTACTGACATGGTGAATCAA ATGACAGAAAAAGTAGGCTTGGTTCACGGGCTGCCTTACGTAGCAGACAGACAGGGCTTT GCTGCCACCTTAGAGCAGGTATATTTTGGAACTTCACATCCAAGATACTATATCTCTGCC AATGTAACTGGTTTCAAATGTGTGACAGGAATGTCTTGTTTAATGAGAAAAGATGTGTTG GATCAAGCAGGAGGACTTATAGCTTTTGCTCAGTACATTGCCGAAGATTACTTTATGGCC AAAGCGATAGCTGACCGAGGTTGGAGGTTTGCAATGTCCACTCAAGTTGCAATGCAAAAC TCTGGCTCATATTCAATTTCTCAGTTTCAATCCAGAATGATCAGGTGGACCAAACTACGA ATTAACATGCTTCCTGCTACAATAATTTGTGAGCCAATTTCAGAATGCTTTGTTGCCAGT TTAATTATTGGATGGGCAGCCCACCATGTGTTCAGATGGGATATTATGGTATTTTTCATG TGTCATTGCCTGGCATGGTTTATATTTGACTACATTCAACTCAGGGGTGTCCAGGGTGGC ACACTGTGTTTTTCAAAACTTGATTATGCAGTCGCCTGGTTCATCCGCGAATCCATGACA ATATACATTTTTTTGTCTGCATTATGGGACCCAACTATAAGCTGGAGAACTGGTCGCTAC AGATTACGCTGTGGGGGTACAGCAGAGGAAATCCTAGATGTATAA Clone variation with respect to NM_003358.1 348 t=>c >OriGene 5' read for NM_003358 unedited
AGCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCACCCCCCT CCGCCCTTTCCTCTCCCCACCTTCCTCTCGCCTCCCGCGCCCCCGCACCGGGCGCCCACC CTGTCCTCCTCCTGCGGGAGCGTTGTCCGTGTTGGCGGCCGCAGCGGGCCGGGCCGGTCC GGCGGGCCGGGGGATGGCGCTGCTGGACCTGGCCTTGGAGGGAATGGCCGTCTTCGGGTT CGTCCTCTTCTTGGTGCTGTGGCTGATGCATTTCATGGCTATCATCTACACCCGATTACA CCTCAACAAGAAGGCAACTGACAAACAGCCTTATAGCAAGCTCCCAGGTGTCTCTCTTCT GAAACCACTGAAAGGGGTAGATCCTAACTTAATCAACAACCTGGAAACATTCTTTGAATT GGATTATCCCAAATATGAAGTGCTCCTTTGTGTACAAGATCATGATGATCCAGCCATTGA TGTATGTAAGAAGCTTCTTGGAAAATATCCAAATGTTGATGCTAGATTGTTTATAGGTGG CAAAAAAGTTGGCATTAATCCTAAAATTAATAATTTAATGCCAGGATATGAAGTTGCAAA GTATGATCTTATATGGATTTGTGATAGTGGAATAAGAGTAATTCCAGATACGCTTACTGA CATGGTGAATCAAATGACAGAAAAAGTAGGCTTGGTTCACGGGCTGCCTTACGTAGCAGA CAGACAGGGCTTTGCTGCCACCTTAGAGCANGTATATTNTGGAACTTCACATCCAAGATA CTATATCTCTGCCAATGTAACTGGTTTCAAATGTGTGACAGGAAATGTCTGTTTTAATGA GAAAGATGTGTTGGNATCAAGCAGGAGGACTTTATAGCTTTTGCTCAGTACATTGCCCGA AGATACTTTATGGCCNAGCGATAGCTGACCGAGGTTGGAGGTTNGCATGTCNCACTCAGT GCATGCC >OriGene 3' read for NM_003358 unedited
GCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCTGTTTGTTTCTGCAAGGCCTA GCTATCTTATTACAATTTATATTTGCTCTGCAACAACTACAAGAGGTAGGCATAAAGCAT GAAAAAGTTTCTCAGTTAGTACCTCGCCATGTGTTGTTCACTGCACAAAACCATTCACTT TGGACATGAATAGTCATTCCTTCTATTATCTTCTAAACTCTAAAGCAAAGCTGCTGCCAC CCGTAAATGTTCTAAAAGAGTGTACTCACAAGTAAGAAACTTTCTCTACTGAAGGATACT GTCACAGAGTTTGTTGCAGAGCATCTATATATATATTTATTTATTTATTTTAAAAAAATA AACAACAATGATGAACGAACCCAGGTTCCTAGAACCAATTCTCTTGATTCTCTACTTCCA CAAAATAAAGTGTATCATTTGGCCAAGACTACAGATGTGTTTTTTTTTTTTTTCACAGAT GCAAGTGCCATGCAAAAATAAATTAAAGAACAGATCCCAAAACATACATGTGATAAAACT ACAGAAGGTAGATTTTTAAAAGCATTTATATAAACATAATTTATAATACTTCTCTTTTTT CCTTTATATACAGTCACAAAGCTGTAGTTATACATCTAGGATTTCCTCTGCTGTACCCCC ACAGCGTAATCTGTAGCGACCAGTTCTCCAGCTTATAGTTGGGTCCCATAATGCAGACAA AAAAATGTATATTGTCATGGATTCGCGGATGAACCANGCGACTGCATAATCAAGTTTGAA AAAACAGTGTGCACCCTGGACACCCTGAGTTGATGTGGCAAAATAAACCTGCCCGCCATG ACCCTGAAAATACCTATTTCCCTCTGACCCTGGGGGCTGCCATCCATATTAAACTGGCAC AAGCATCCGAATTGGCTCACAATTATGACCAGAAACATGTATCCGANTTGGCCCACCGAT C |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003358 |
| Insert Size | 1710 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003358.1, NP_003349.1 |
| RefSeq Size | 1637 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 7357 |
| UniProt ID | Q16739 |
| Domains | Glycos_transf_2 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | This gene encodes an enzyme that catalyzes the first glycosylation step in the biosynthesis of glycosphingolipids, which are membrane components containing lipid and sugar moieties. The product of this reaction is glucosylceramide, which is the core structure of many glycosphingolipids. [provided by RefSeq, Dec 2014] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Orthoxenografts of testicular germ cell tumors demonstrate genomic changes associated with cisplatin resistance and identify PDMP as a re-sensitizing agent
,Piulats, JM;Vidal, A;García-Rodríguez, FJ;Muñoz, C;Nadal, M;Moutinho, C;Martínez-Iniesta, M;Mora, J;Figueras, A;Guinó, E;Padullés, L;Aytés, À;Mollevi, DG;Puertas, S;Martínez-Fernández, C;Castillo, W;Juliachs, M;Moreno, V;Muñoz, P;Stefanovic, M;Pujana, MA;Condom, E;Esteller, M;Germà-LLuch, JR;Capella, G;Farré, L;Morales, A;Viñals, F;Garcia Del Muro, X;Cerón, J;Villanueva, A;,
Clin. Cancer Res.
,PubMed ID 29618620
[UGCG]
|
|
The UDP-glucose ceramide glycosyltransferase (UGCG) and the link to multidrug resistance protein 1 (MDR1)
,null,
BMC Cancer
,PubMed ID 29409484
[UGCG]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206288 | UGCG (Myc-DDK-tagged)-Human UDP-glucose ceramide glucosyltransferase (UGCG) |
CNY 5,488.00 |
|
| RC206288L1 | Lenti ORF clone of Human UDP-glucose ceramide glucosyltransferase (UGCG), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC206288L2 | Lenti ORF clone of Human UDP-glucose ceramide glucosyltransferase (UGCG), mGFP tagged |
CNY 7,888.00 |
|
| RC206288L3 | Lenti ORF clone of Human UDP-glucose ceramide glucosyltransferase (UGCG), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC206288L4 | Lenti ORF clone of Human UDP-glucose ceramide glucosyltransferase (UGCG), mGFP tagged |
CNY 7,888.00 |
|
| RG206288 | UGCG (tGFP-tagged) - Human UDP-glucose ceramide glucosyltransferase (UGCG) |
CNY 7,088.00 |
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