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TATA binding protein (TBP) (NM_003194) Human Untagged Clone
CAT＃: SC118124
TBP (untagged)-Human TATA box binding protein (TBP), transcript variant 1
CNY 3,656.00
CNY 7,220.00
| Cited in 1 publication. |
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GTF2D; GTF2D1; HDL4; SCA17; TFIID |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118124 sequence for NM_003194 edited (data generated by NextGen Sequencing)
ATGGATCAGAACAACAGCCTGCCACCTTACGCTCAGGGCTTGGCCTCCCCTCAGGGTGCC ATGACTCCCGGAATCCCTATCTTTAGTCCAATGATGCCTTATGGCACTGGACTGACCCCA CAGCCTATTCAGAACACCAATAGTCTGTCTATTTTGGAAGAGCAACAAAGGCAGCAGCAG CAACAACAACAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAG CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGGCAGTGGCAGCTGCA GCCGTTCAGCAGTCAACGTCCCAGCAGGCAACACAGGGAACCTCAGGCCAGGCACCACAG CTCTTCCACTCACAGACTCTCACAACTGCACCCTTGCCGGGCACCACTCCACTGTATCCC TCCCCCATGACTCCCATGACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGG ATTGTACCGCAGCTGCAAAATATTGTATCCACAGTGAATCTTGGTTGTAAACTTGACCTA AAGACCATTGCACTTCGTGCCCGAAACGCCGAATATAATCCCAAGCGGTTTGCTGCGGTA ATCATGAGGATAAGAGAGCCACGAACCACGGCACTGATTTTCAGTTCTGGGAAAATGGTG TGCACAGGAGCCAAGAGTGAAGAACAGTCCAGACTGGCAGCAAGAAAATATGCTAGAGTT GTACAGAAGTTGGGTTTTCCAGCTAAGTTCTTGGACTTCAAGATTCAGAATATGGTGGGG AGCTGTGATGTGAAGTTTCCTATAAGGTTAGAAGGCCTTGTGCTCACCCACCAACAATTT AGTAGTTATGAGCCAGAGTTATTTCCTGGTTTAATCTACAGAATGATCAAACCCAGAATT GTTCTCCTTATTTTTGTTTCTGGAAAAGTTGTATTAACAGGTGCTAAAGTCAGAGCAGAA ATTTATGAAGCATTTGAAAACATCTACCCTATTCTAAAGGGATTCAGGAAGACGACGTAA Clone variation with respect to NM_003194.4 >OriGene 5' read for NM_003194 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGCCTGGGCC GCCGGCTGTTTAACTTCGCTTCCGCTGGCCCATAGTGATCTTTGCAGTGACCCAGCAGCA TCACTGTTTCTTGGCGTGTGAAGATAACCCAAGGAATTGAGGAAGTTGCTGAGAAGAGTG TGCTGGAGATGCTCTAGGAAAAAATTGAATAGTGAGACGAGTTCCAGCGCAAGGGTTTCT GGTTTGCCAAGAAGAAAGTGAACATCATGGATCAGAACAACAGCCTGCCACCTTACGCTC AGGGCTTGGCCTCCCCTCAGGGTGCCATGACTCCCGGAATCCCTATCTTTAGTCCAATGA TGCCTTATGGCACTGGACTGACCCCACAGCCTATTCAGAACACCAATAGTCTGTCTATTT TGGAAGAGCAACAAAGGCAGCAGCAGCAACAACAACAGCAGCAGCAGCAGCAGCAGCAGC AGCAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC AGCAGCAACAGGCAGTGGCAGCTGCAGCCGTTCAGCAGTCAACGTCCCAGCAGGCAACAC AGGGAACCTCAGGCCAGGCACCACAGCTCTTCCACTCACAGACTCTCACAACTGCACCCT TGCCGGGCACCCACTCCACTGTATCCCTCCCCCATGACTCCCATGACCCCCATCACTCCT GCCACGCCAGCTTTGGAGAGTTCTGGGATTGTACCGCAGCTGCAAATATTGTATCCACAG TGAATCTTGGGTGTAACTTGACCTAAAGACCTTGCACTTCGTGCCCGACCGCCGATTATA TCCCAGCGGGTGGCTGCGGTATCATGAGGAATAGAAG >OriGene 3' read for NM_003194 unedited
TCTTGGACCCGGCCGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATAAACTGCATT TATTGTACAGAGTACTCCTGAAGAAAGAAAAAAATATGTACAGCCATTCATTTTCATTAA CACATATATTCTGTCCTGCAATACTGGAGAGGTGGAATGTGTCTGGCACAGAAATAACCC CTAGGAGTTATAAATTAGAAAAACAACACTTTTAAAAAAGATTTTTACTTTTCTGGTAGA AATATAAAAACTGTGGTTCATGGGGAAAAACATTAAAATTAAAAAGTCCAATCAATTATA GAGGTGGCTTTAACACTTAAAGTTTTCTCCCTCAAACCAACTTGTCAACAGCAGTGTTTA AAATCTACATATAAATAAATGGGCAGCGCTGCCCAGATAGCAGCACGGTATGAGCAACTC ACAGTCACGCTGCGCGGTGTTCTCAGTGCACAAATAATGCCCCTTCCCGGCATCCCGCGG TGGGCACTTACAGAAGGGCATCACCTGGTGCCACACCCTGCAACTCAACATCCATCTTCT CACAACACCACCATTTAAAGGTACCAAAACAAACTGATTTGTCTAAAAAAAAAAACACAA GGGGGTGGGGGAGGCATGGGTACTTGAAAGCCCTTACTTCGTCTTCCTGAATCCCTATAA ATATGGTAGATGCTCCCAAATGCTCACAAATTCCTGCCCTGACTTTTACACCTGGTAATA CAACTTTATCCGAAACCCAACTCTCGCACAACCATTCTGGGTTTGATCCTTCCTGCACAT TATACCCAGAAAATACCGTCGGCCACATACCACCTACTATGCGCTTGGGTGTACCCCCTG CACTATTCACCCTTATGAGCATCCCCCATTTCCACTCCCCGCTCTAATCCCCACCTCTGA CTCTCTCAACCTCACTGGCAACGCCACTCTCTGTCTACTCTCTCATTTAACCCGTGNACC GACCCACCTGTATACCCACTGTCCCTGCGCCTACTATATAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003194 |
| Insert Size | 1950 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003194.3, NP_003185.1 |
| RefSeq Size | 1867 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 6908 |
| UniProt ID | P20226 |
| Domains | TBP |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Basal transcription factors, Huntington's disease |
| Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Increased global transcription activity as a mechanism of replication stress in cancer
,Kotsantis, P;Silva, LM;Irmscher, S;Jones, RM;Folkes, L;Gromak, N;Petermann, E;,
Nat Commun
,PubMed ID 27725641
[TBP]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210727 | TBP (Myc-DDK-tagged)-Human TATA box binding protein (TBP), transcript variant 1 |
CNY 3,656.00 |
|
| RC210727L1 | Lenti ORF clone of Human TATA box binding protein (TBP), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
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| RC210727L2 | Lenti ORF clone of Human TATA box binding protein (TBP), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RC210727L3 | Lenti ORF clone of Human TATA box binding protein (TBP), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210727L4 | Lenti ORF clone of Human TATA box binding protein (TBP), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210727 | TBP (tGFP-tagged) - Human TATA box binding protein (TBP), transcript variant 1 |
CNY 5,256.00 |
|
| SC322062 | TBP (untagged)-Human TATA box binding protein (TBP), transcript variant 1 |
CNY 3,656.00 |
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