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SRP54 (NM_003136) Human Untagged Clone
CAT＃: SC118182
SRP54 (untagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 1
CNY 5,640.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SCN8 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118182 sequence for NM_003136 edited (data generated by NextGen Sequencing)
ATGGTTCTAGCAGACCTTGGAAGAAAAATAACATCAGCATTACGCTCGTTGAGCAATGCC ACCATTATCAATGAAGAGGTATTGAATGCTATGCTAAAAGAAGTCTGTACCGCTTTGTTG GAAGCAGATGTTAATATTAAACTAGTGAAGCAACTAAGAGAAAATGTTAAGTCTGCTATT GATCTTGAAGAGATGGCATCTGGTCTTAACAAAAGAAAAATGATTCAGCATGCTGTATTT AAAGAACTTGTGAAGCTTGTAGACCCTGGAGTTAAGGCATGGACACCCACTAAAGGAAAA CAAAATGTGATTATGTTTGTTGGATTGCAAGGGAGTGGTAAAACAACAACATGTTCAAAG CTAGCATATTATTACCAGAGGAAAGGTTGGAAGACCTGTTTAATATGTGCAGACACATTC AGAGCAGGGGCTTTTGACCAACTAAAACAGAATGCTACCAAAGCAAGAATTCCATTTTAT GGAAGCTATACAGAAATGGATCCTGTCATCATTGCTTCTGAAGGAGTAGAGAAATTTAAA AATGAAAATTTTGAAATTATTATTGTTGATACAAGTGGCCGCCACAAACAAGAAGACTCT TTGTTTGAAGAAATGCTTCAAGTTGCTAATGCTATACAACCTGATAACATTGTTTATGTG ATGGATGCCTCCATTGGGCAGGCTTGTGAAGCCCAGGCTAAGGCTTTTAAAGATAAAGTA GATGTAGCCTCAGTAATAGTGACAAAACTTGATGGCCATGCAAAAGGAGGTGGTGCACTC AGTGCAGTCGCTGCCACAAAAAGTCCGATTATTTTCATTGGTACAGGGGAACATATAGAT GACTTTGAACCTTTCAAAACACAGCCTTTTATTAGCAAACTTCTTGGTATGGGCGACATT GAAGGACTGATAGATAAAGTCAACGAGTTGAAGTTGGATGACAATGAAGCACTTATAGAG AAGTTGAAACATGGTCAGTTTACGTTGCGAGACATGTATGAGCAATTTCAAAATATCATG AAAATGGGCCCCTTCAGTCAGATCTTGGGGATGATCCCTGGTTTTGGGACAGATTTTATG AGCAAAGGAAATGAACAGGAGTCAATGGCAAGGCTAAAGAAATTAATGACAATAATGGAT AGTATGAATGATCAAGAACTAGACAGTACGGATGGTGCCAAAGTTTTTAGTAAACAACCA GGAAGAATCCAAAGAGTAGCAAGAGGATCGGGTGTATCAACAAGAGATGTTCAAGAACTT TTGACACAATATACCAAGTTTGCACAGATGGTAAAAAAGATGGGAGGTATCAAAGGACTT TTCAAAGGTGGCGACATGTCTAAGAATGTGAGCCAGTCACAGATGGCAAAATTGAACCAA CAAATGGCCAAAATGATGGATCCTAGGGTTCTTCATCACATGGGTGGTATGGCAGGACTT CAGTCAATGATGAGGCAGTTTCAACAGGGTGCTGCTGGCAACATGAAAGGCATGATGGGA TTCAATAATATGTAA Clone variation with respect to NM_003136.3 >OriGene 5' read for NM_003136 unedited
TGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGCTCCGCCTAGTCACTTTT CTTAAGTGGCTCGTCGAGGCCTGACTTCTTCCCCGAATCACGTCCCTAGACAGCCTCCTA TTTTACCACTAACTTTACTCCTGCAGTTATTCAGCGGTAGGAAACTGAAACCAAAAACCA GTGTAAGCAAGTAAACATCTAAACTGTTTCAGGAGCCGCGTAGAAGGAACGCGGCGGTGT GCCCCGGAAGCGGAAGTAGATTCTCCTATAGAAAGGCTGGACTACGCGGAGTGGTGACGT TTCCTCATTGGGCGGAAGGTTCGCTGGCACTCCGTTGGTCTTCCAGCTGGTGGGAGTTGA CGACGTGGTGCTGGGCGTTGGGACCCTACTTTATCTAGTTCGGGAAGTTGGGTTGTGGGG TCATACCTGTCTGTCTGCTCCCAGCTTTCTTGGGTTTCTTCCGACGGCGTGGGGCCTCGC TAAGGAATTCCCGGCCCCTCAGGGCCACGGCTTTAGCGGTGTCTTTTGCGAGTTCTTCGT AAGTACATCTTAAAGCTGTCAAGATGGTTCTAGCAGACCTTGGAAGAAAAATAACATCAG CATTCCGCTCGTTGAGCAATGCCACCATTATCAATGAAGAGGTATTGAATGCTATGCTAA AAGAAGTCTGTACCGCTTTGTTGGAAGCAGATGTTAATATTAAACTAGTGAAGCAACTAA AGAAATGTTAAGTCTGCTATTGATCTTGAAGAGATGCATCTGGTCTTAACAAAGAAAATG ATTCAGCATGCTGTATTAAAGAACTTGTGAAGCTGTAACCCTGNANTNAGGCATGGACAC CACTAAAGAAACANATGTGATATATGTTGTTGGATGCAAGGGAGTGGTAAACAANCACTG NTCAAA >OriGene 3' read for NM_003136 unedited
NCCCCCACGCCCTCCNCCCCCCCCCCCCNNCCTTTCNCTTGTTTTGCNACGCGCGCCGCC TCCCACGATCGAGGTTCTTTTCTCTTTTTTTTCTTTTTATTCTACAGCACTTTAATTGAA ACATTATTTAACTACTGACCAGGACTGTTTACAAATGCAATGCTTGGCCTTCGAGATAAT TAAAAACCTTTCAGTACTAAAATTTTACATCATGATTTAACTTAAGTGTTACGATGGTGA AAACAGAACACAATCTAAAGCACACATATAATGATTTCCACACAAACCATGTATTTCTCC CTTATATTAAAGGGAGGAACCGATGAAGGAAAAAGCAAACGGAGGGGAAAAGCGGGCGAG ATAAAAAGGAAAGAGTGCATGATAAGCAACAAAAATACACTTTCCCCCCAATTCGCACAA AGAATGGAAACGCTGAGGTCTCCACCCAAATTAGGTATCCAACTGAGTCAGTTTATATTA AGGCATTTCCTTTACATATTATTGAAATCCCATCATGCCTTTCATGTTGCCAGCAGAACC CTGCTGAAACTGCCTCACCATTGACTGAAGTCTTGCCCTACCACCCCATGTGATGAAAAA ACCCTAGGATTCATTCATTTTTTGGCCATTCGTTGGTTCACATTTGCATCTGTGACTGCC TCACATTTTTACACCATGTTCGCACCTTTTGAAAGCCCTTTTGATACCTCCCATTTTTTT TTACAATCTGCGCAAACTTGGTAACACTGCGACAAAAGCTTTTGGAACAACCTCTGCTGA AACACCCGATCCTTTCGCTCCCCTTTGGATCCTCNCCGGCCGTTCACTCAAATCTTTTGG CACTTCCCGTCCGGCCATACCTTTGAACTTCCTAACACGCCATTTTCGTCCTTAATTTTT TTAACCTCTGCCTTGAATCCCTGGCATATTCCTTTGGCTCAATACACCTTGCCCAAAACA CCGCGTATATCCCCAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003136 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003136.2, NP_003127.1 |
| RefSeq Size | 2164 bp |
| RefSeq ORF | 1515 bp |
| Locus ID | 6729 |
| UniProt ID | P61011 |
| Domains | SRP54, AAA, SRP_SPB |
| Protein Pathways | Protein export |
| Gene Summary | Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Intragenic Transcriptional cis-Antagonism Across SLC6A3
,Zhao, Y;Yu, J;Zhao, J;Chen, X;Xiong, N;Wang, T;Qing, H;Lin, Z;,
Mol. Neurobiol.
,PubMed ID 30259411
[SRP54]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201658 | SRP54 (Myc-DDK-tagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 1 |
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| RC201658L1 | Lenti-ORF clone of SRP54 (Myc-DDK-tagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 1 |
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| RC201658L2 | Lenti-ORF clone of SRP54 (mGFP-tagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 1 |
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| RC201658L3 | Lenti-ORF clone of SRP54 (Myc-DDK-tagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 1 |
CNY 6,460.00 |
|
| RC201658L4 | Lenti-ORF clone of SRP54 (mGFP-tagged)-Human signal recognition particle 54kDa (SRP54), transcript variant 1 |
CNY 8,032.00 |
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| RG201658 | SRP54 (tGFP-tagged) - Human signal recognition particle 54kDa (SRP54), transcript variant 1 |
CNY 5,040.00 |
