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MCP1 (CCL2) (NM_002982) Human Untagged Clone
CAT＃: SC118317
CCL2 (untagged)-Human chemokine (C-C motif) ligand 2 (CCL2)
CNY 1,800.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118317 sequence for NM_002982 edited (data generated by NextGen Sequencing)
ATGAAAGTCTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCCAA GGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTATAACTTCACCAAT AGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAGAATCACCAGCAGCAAGTGTCCC AAAGAAGCTGTGATCTTCAAGACCATTGTGGCCAAGGAGATCTGTGCTGACCCCAAGCAG AAGTGGGTTCAGGATTCCATGGACCACCTGGACAAGCAAACCCAAACTCCGAAGACTTGA Clone variation with respect to NM_002982.3 >OriGene 5' read for NM_002982 unedited
GGGGTAGATTTGTAACACGATTCACTATAGGGCGGCCGCGAAATCGGCACGAGGTAACCC AGAACATCCAATTCTCAAACTGAAGCTCGCACTCTCGCCTCCAGCATGAAAGTCTCTGCC GCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCCAAGGGCTCGCTCAGCCA GATGCAATCAATGCCCCAGTCACCTGCTGTTATAACTTCACCAATAGGAAGATCTCAGTG CAGAGGCTCGCGAGCTATAGAAGAATCACCAGCAGCAAGTGTCCCAAAGAAGCTGTGATC TTCAAGACCATTGTGGCCAAGGAGATCTGTGCTGACCCCAAGCAGAAGTGGGTTCAGGAT TCCATGGACCACCTGGACAAGCAAACCCAAACTCCGAAGACTTGAACACTCACTCCACAA CCCAAGAATCTGCAGCTAACTTATTTTCCCCTAGCTTTCCCCAGACACCCTGTTTTATTT TATTATAATGAATTTTGTTTGTTGATGTGAAACATTATGCCTTAAGTAATGTTAATTCTT ATTTAAGCTATTGATGTTTTAAGTTATCTTTCATGGTACTAGCGCTTTCTACATACAGAG ACTTGGGGGAAATTGCTTTTCCTCTTGAACCACAGTTCTACCCCCTGGGATGCTTTGAGG GTCTTTGCAAGATCATTAATACAAAGAATTTTTTTTAACATTCCCAAGCATTGCTAAAAT AATATTGCGGAAATGAATATTTTGGAACCATTACCCCAAACAAATATATTTTTTGTACAA AAAAAAAAAAAAAACCTTACTTCTAAATTGAGCCCCGGCCCATTCTTTCCCCTGACCACA CCCCCGTGGGCTCCCTGTGAACCTCCCCCAATGCCCTTCCTGNCCCTGAAAGTTGCCATT CCATGCGCCACACCCCTGGCT >OriGene 3' read for NM_002982 unedited
CAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGTACAAAAATATATTTATTTGGTGTAA TAGTTACAAAATATTCATTTCCACAATAATATTTTAGCAATGCATTGGAATGTTAAAAAA AATTCTTTGTATTAATGATTCTTGCAAAGACCCTCAAAACATCCCAGGGGTAGAACTGTG GTTCAAGAGGAAAAGCAATTTCCCCAAGTCTCTGTATCTAAAAAACACTAGTACCATGAA AGATAAACTTAAAACATCAATAACTTAAATAAGAATTAACATTACTTAAGGCATAATGTT TCACATCAACAAACAAAATTCATTATAATAAAATAAAACAGGGTGTCTGGGGAAAGCTAG GGGAAAATAAGTTAGCTGCAGATTCTTGGGTTGTGGAGTGAGTGTTCAAGTCTTCGGAGT TTGGGTTTGCTTGTCCAGGTGGTCCATGGAATCCTGAACCCACTTCTGCTTGGGGTCAGC ACAGATCTCCTTGGCCACAATGGTCTTGAAGATCACAGCTTCTTTGGGACACTTGCTGCT GGAGATTCTTCTATAGCTCGCGAGCCTCTGCACTGAGATCTTCCTATTGGTGAAGTTATA ACAGCAGGTGACTGGNGCATTGATTGCATCTGGCTGAGCGAGCCCTTGGGGAATGAAGGT GGCTGCTATGAGCAGCAGGCACATGATGGCCGCAGATACTCTCATGCNTGNAGCGAGAGT GCCAGCCTTAGTTTGAGAATTGGATGTTTCCTGGGTTACCTCGTGCCCGATTTCCCGGCC GCCCTATAGTGAGTCGTATTACAAAATCCTGACGGTTCACTAAACGAGCTCTGCCTATTT AGACTNCCACCGACACGCCTACGNCCATTTGTGTAACGGGGCCGGGTTTTACGACTTTCG GAAGTCCCGTGTTTTGTTGCAAAAAATTCACTGCGTCATGGGTGAGACTCGAATCCCGGG GGTAAACGCTTCAGCCCTTGTGTCTGCAAAACGTTACATGTATG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002982 |
| Insert Size | 700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002982.3, NP_002973.1 |
| RefSeq Size | 760 bp |
| RefSeq ORF | 300 bp |
| Locus ID | 6347 |
| UniProt ID | P13500 |
| Domains | IL8 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Chemokine signaling pathway, Cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway |
| Gene Summary | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. [provided by RefSeq, Aug 2020] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Arctigenin suppresses transforming growth factor-ß1-induced expression of monocyte chemoattractant protein-1 and the subsequent epithelial-mesenchymal transition through reactive oxygen species-dependent ERK/NF-?B signaling pathway in renal tubular epithe
,Li, A;Wang, J;Zhu, D;Zhang, X;Pan, R;Wang, R;,
Free Radic. Res.
,PubMed ID 25968940
[CCL2]
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