RFC1 (NM_002913) Human Untagged Clone
CAT#: SC118344
RFC1 (untagged)-Human replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 1
CNY 11,168.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | A1; CANVAS; MHCBFB; PO-GA; RECC1; RFC; RFC140 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_002913, the custom clone sequence may differ by one or more nucleotides
ATGGACATTCGGAAATTCTTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGA ATGAGAAAACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAATAG CTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATCTATGATTCAGAT TCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCAGAAAAACTGCCAGTATCTTCTA AACCTGGTAAAATTTCACGGCAGGATCCTGTTACATACATTTCAGAAACAGATGAAGAAGATGACTTTAT GTGTAAGAAGGCGGCCTCTAAATCAAAAGAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAAC ATGAAAAAGAATGAAGAAAACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACAT CAGTACTTGATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGAAA AGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAATTACAGCTTGAT GAAGATGCGGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAGTTTGCCAGAACATTAGCCATGTTGGATG AAGAACCCAAGACCAAAAAGGCTCGAAAGGACACAGAAGCGGGAGAAACGTTTTCATCTGTCCAAGCCAA TTTAAGTAAAGCAGAAAAACATAAATATCCTCATAAAGTAAAAACAGCACAAGTTTCAGATGAAAGAAAG AGCTACAGTCCTAGGAAGCAAAGTAAATATGAAAGTTCAAAAGAATCTCAGCAACATTCCAAGTCATCAG CTGACAAAATAGGAGAAGTCTCTTCTCCCAAGGCCAGTTCTAAGCTGGCAATTATGAAAAGAAAAGAAGA GAGCTCTTATAAAGAAATAGAGCCTGTGGCCTCAAAAAGAAAAGAAAATGCCATTAAATTGAAAGGAGAG ACAAAAACTCCTAAGAAAACCAAAAGTTCTCCAGCTAAAAAAGAGTCTGTAAGTCCTGAAGATTCTGAAA AGAAACGCACTAATTATCAAGCTTATCGAAGCTACTTAAATCGAGAAGGTCCCAAGGCTCTGGGCTCCAA AGAAATACCAAAGGGAGCTGAAAATTGCTTGGAAGGCCTTATATTTGTAATCACAGGCGTGCTGGAGTCT ATTGAACGAGATGAGGCCAAGTCTCTAATTGAACGTTATGGGGGAAAAGTAACAGGAAATGTCAGCAAGA AAACAAATTATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGGCCGCAGCCTTGGGGAC AAAAATTATTGATGAAGATGGCCTGTTGAATCTGATTCGGACTATGCCAGGCAAGAAATCCAAGTATGAA ATAGCAGTTGAAACTGAGATGAAGAAAGAGTCCAAACTGGAGAGAACACCCCAAAAAAATGTCCAAGGAA AAAGAAAAATTAGTCCATCTAAAAAGGAATCAGAATCTAAAAAGAGCAGGCCGACTTCCAAAAGGGACAG TTTGGCAAAGACAATAAAAAAGGAAACAGATGTGTTTTGGAAAAGCCTGGATTTCAAGGAGCAGGTGGCT GAGGAGACAAGTGGTGACAGCAAGGCTAGGAATTTGGCTGATGACAGCAGTGAAAACAAAGTGGAAAATT TGCTCTGGGTGGATAAATATAAGCCAACCTCGCTCAAGACCATAATTGGACAGCAAGGTGACCAGAGCTG TGCCAACAAACTCCTACGCTGGCTCCGAAACTGGCAAAAGAGTTCTTCCGAAGATAAAAAACACGCAAAG TTTGGTAAATTTTCCGGCAAAGATGATGGCTCTAGTTTTAAAGCAGCGTTGCTGTCAGGCCCTCCTGGTG TTGGCAAAACCACCACAGCTTCCCTGGTGTGTCAGGAGTTGGGATACAGCTACGTGGAACTGAATGCAAG TGACACCCGGAGTAAGAGCAGTTTGAAGGCGATTGTTGCTGAGTCACTGAACAATACCAGCATCAAAGGC TTTTATTCAAATGGAGCAGCCTCTTCAGTAAGCACGAAACATGCTCTCATCATGGATGAAGTAGATGGCA TGGCAGGCAATGAGGATAGGGGAGGAATTCAGGAATTAATTGGCCTGATAAAACATACTAAAATTCCCAT TATTTGTATGTGCAATGATAGAAATCATCCCAAGATTCGCTCTCTGGTTCATTATTGTTTTGATCTTCGT TTTCAAAGACCTCGGGTTGAACAGATTAAGGGTGCTATGATGTCTATTGCATTTAAAGAAGGTTTAAAGA TTCCCCCTCCAGCTATGAATGAAATAATTTTGGGAGCCAATCAAGATATCAGACAGGTTTTACATAATCT GAGTATGTGGTGTGCACGAAGTAAAGCATTAACCTATGACCAGGCCAAAGCTGATTCTCACAGAGCCAAA AAGGATATCAAAATGGGCCCATTTGATGTTGCCCGGAAAGTGTTTGCAGCTGGAGAGGAGACTGCTCACA TGTCACTTGTGGACAAGTCAGATCTCTTTTTTCATGATTATTCAATAGCACCCCTCTTCGTCCAGGAAAA TTACATACACGTGAAGCCTGTAGCAGCAGGGGGTGACATGAAAAAGCACCTGATGCTTTTAAGCAGAGCA GCAGACAGCATATGCGATGGTGACCTAGTGGACAGCCAGATCCGGAGTAAGCAAAACTGGAGTCTTCTGC CTGCGCAGGCCATTTATGCCAGTGTTCTTCCTGGAGAGTTGATGAGGGGGTACATGACCCAGTTTCCCAC CTTCCCAAGCTGGCTGGGGAAGCACTCGTCTACAGGCAAACATGATCGTATTGTTCAGGACCTGGCCTTG CATATGAGTCTCAGAACTTACTCCAGCAAAAGGACTGTAAACATGGATTATCTGTCGCTTCTAAGGGATG CACTTGTACAGCCCTTGACCTCACAAGGAGTAGACGGAGTACAGGATGTTGTTGCACTTATGGACACATA TTATTTGATGAAAGAAGACTTTGAGAATATCATGGAAATCAGCAGCTGGGGTGGCAAACCTAGTCCCTTT TCAAAGCTGGATCCCAAGGTGAAAGCAGCCTTCACAAGAGCTTACAATAAGGAAGCCCACCTTACTCCAT ACTCACTTCAAGCTATAAAGGCATCTAGACACAGCACAAGCCCATCCCTGGATTCGGAATACAATGAAGA ATTAAATGAAGATGACTCTCAATCTGATGAGAAAGACCAAGATGCTATAGAAACTGATGCCATGATCAAG AAAAAGACAAAATCTTCAAAGCCTTCAAAACCAGAAAAAGATAAGGAGCCCAGAAAAGGAAAAGGAAAAA GTTCGAAGAAATGA >OriGene 5' read for NM_002913 unedited
GGGGTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTGCACT TCGCGGGAGAAGTTGTTGGCGCGAATGGATCCTGAGCCTCGATAACAGATTCCTCAACCG GCCCACCCGCCAGCCAGCCAGCGCCTTCATCCTGGGGCTGCGATGGACATTCGGAAATTC TTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGAATGAGAAA ACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAAT AGCTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATC TATGATTCAGATTCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCA GAAAAACTGCCAGTATCTTCTAAACCTGGTAAAATTTCACGGCAGGATCCTGTTACATAC ATTTCAGAAACAGATGAAGAAGATGACTTTATGTGTAAGAAGGCGGCCTCTAAATCAAAA GAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAACATGAAAAAGAATGAAGAA AACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACATCAGTACTT GATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGA AAAGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAA TTACAGCTTGATGAAGATGCGGAGCTGCAGAGGCAGTTGCATGAAGATGAAGAGTTTGCC CAGACATTAGCCATGTTGGATGAAGACCCAAGACCAAAAAGCTCGAAAGACACAGAAGCG A >OriGene 3' read for NM_002913 unedited
CGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTAATGTGGAACATTTTTATTCCTAAT TTATACTTTNGAAAGATATCTGACAATGTACAAAGAATACCCAGTTCTAGGTGGTGACTG TTTTGTATAATCATTTATTTACAAAATTTCACCACATAATAGGAACTGCCTTTAAAAAAA ATCAGCATTGGTCCTATAAAGGCCATTAGGATAAATTCTTAAGCCTACAAAAGACGCCTT ATTACTACTGCTCCCAGGCTCATTATTGTTAACTAAAATCTGACTCCAAAGCCCAGGTGT AGTTTCTAGTTCCAATTCTACAGTCATTCAGAAGCACGTCTAACTAGATTGACAGAGGAC AGTGGAGGACCCAAGCAGTCCTATCAACCTCTATAAGAGGTGTACATAAGCCTACTGCTC ATACCCTTCTAGCCATACCACCCTTTATTTATAATGGGACACCAGGTCATCCCATTATGC TAAAACATGGTTGCTCTGGGAAAAAACAAGGCTTTCTCTAGACCAGCTGGACTGGTCAGG AGGGAGAGTAAAAAGTGGCTGTCGCTAGTGAAAAATGGTTTCATTTCTTCGAACTTTTCC TNTTCCTTTCTGGGCTCCTTATCTTTTTTCTGGTTTGAAGGCTTTGAAGATTTTGTCTTT TTTCTTGATCATGGCATCCAGTTTCTATAGCATCCTAGCTCTTTTCTCATTCAGATTAGA AAGTCATCCTAATTTAATTTCTTCATTGTATTCCCGAATCCAAGGGATGGGCTTGTGCCT GGCTCTAAAAGCCTTTATACCTTGAAGTGAGTTTGAAGTAAGGTCGGCCTTCTTTATTGA AGCCTCTGAGAAAGCTGTTTTACCTTGGAACCATCTTGAAAAGAACTATGTTGCCGCCCA CCTGTGATTTCCAGAATTCCAAGTCTTCTTTATAAATAATGGGCCCTTATGCAAAACATC TGATCTCCGTTCTCTTTGGGAGCAAGCTTGGAAGGGCCTCCTT |
Restriction Sites | NotI-NotI |
ACCN | NM_002913 |
Insert Size | 4170 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002913.3, NP_002904.3 |
RefSeq Size | 4896 bp |
RefSeq ORF | 4896 bp |
Locus ID | 5981 |
UniProt ID | P35251 |
Domains | BRCT, AAA, AAA |
Protein Families | Transcription Factors |
Protein Pathways | DNA replication, Mismatch repair, Nucleotide excision repair |
Gene Summary | This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. |
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Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208240 | RFC1 (Myc-DDK-tagged)-Human replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 1 |
CNY 7,984.00 |
|
RC208240L3 | Lenti-ORF clone of RFC1 (Myc-DDK-tagged)-Human replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 1 |
CNY 13,300.00 |
|
RC208240L4 | Lenti-ORF clone of RFC1 (mGFP-tagged)-Human replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 1 |
CNY 13,300.00 |
|
RG208240 | RFC1 (tGFP-tagged) - Human replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 1 |
CNY 12,540.00 |