PSMD2 (NM_002808) Human Untagged Clone
CAT#: SC118429
PSMD2 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2)
CNY 6,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | P97; RPN1; S2; TRAP2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC118429 sequence for NM_002808 edited (data generated by NextGen Sequencing)
ATGGAGGAGGGAGGCCGGGACAAGGCGCCGGTGCAGCCCCAGCAGTCTCCAGCGGCNNNN CCCGGCGGCACGGACGAGAAGCCGAGCGGCAAGGAGCGGCGGGATGCCGGGGACAAGGAC AAAGAACAGGAGCTGTCTGAAGAGGATAAACAGCTTCAAGATGAACTGGAGATGCTCGTG GAACGACTAGGGGAGAAGGATACATCCCTGTATCGACCAGCGCTGGAGGAATTGCGAAGG CAGATTCGTTCTTCTACAACTTCCATGACTTCAGTGCCCAAGCCTCTCAAATTTCTGCGT CCACACTATGGCAAACTGAAGGAAATCTATGAGAACATGGCCCCTGGGGAGAATAAGCGT TTTGCTGCTGACATCATCTCCGTTTTGGCCATGACCATGAGTGGGGAGCGTGAGTGCCTC AAGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCATGGGGTCATGAGTATGTCAGG CATCTGGCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGAGAAGGTCCAG CGGGAGCCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCA GAGCATGAGGCTTGCGACCTGCTTATGGAAATTGAGCAGGTGGACATGCTGGAGAAGGAC ATTGATGAAAATGCATATGCAAAGGTCTGCCTTTATCTCACCAGTTGTGTGAATTACGTG CCTGAGCCTGAGAACTCAGCCCTACTGCGTTGTGCCCTGGGTGTGTTCCGAAAGTTTAGC CGCTTCCCTGAAGCTCTGAGATTGGCATTGATGCTCAATGACATGGAGTTGGTAGAAGAC ATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGATGGCATTCATGCTAGGCCGG CATGGGGTGTTCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCTGACAGAGATC ATGTCCAATGTACAGCTCAACAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATG GAGCCCAAGGTGCCTGATGACATCTACAAAACCCACCTAGAGAACAACAGGTTTGGGGGC AGTGGCTCTCAGGTGGACTCTGCCCGCATGAACCTGGCCTCCTCTTTTGTGAATGGCTTT GTGAATGCAGCTTTTGGCCAAGACAAGCTGCTAACAGATGATGGCAACAAATGGCTTTAC AAGAACAAGGACCACGGAATGTTGAGTGCAGCTGCATCTCTTGGGATGATTCTGCTGTGG GATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGTACTCCTCTGAGGACTACATT AAGTCAGGAGCTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAATGAGTGTGAC CCTGCTCTGGCACTGCTCTCAGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGT TCCATCTTTGGGCTAGGCTTGGCTTATGCTGGCTCAAATCGTGAAGATGTCCTAACACTG CTGCTGCCTGTGATGGGAGATTCAAAGTCCAGCATGGAGGTGGCAGGTGTCACAGCTTTA GCCTGTGGAATGATAGCAGTAGGGTCCTGCAATGGAGATGTAACTTCCACTATCCTTCAG ACCATCATGGAGAAGTCAGAGACTGAGCTCAAGGATACTTATGCTCGTTGGCTTCCTCTT GGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATCGAGGCAATCCTGGCTGCACTG GAGGTTGTGTCAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGATGTGTGTGCATAT GCAGGCTCTGGGAATGTGCTGAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACACTTT GACTCCAAAGAGAAGGAGGAAGACAAAGACAAGAAGGAAAAGAAAGACAAGGACAAGAAG GAAGCCCCTGCTGACATGGGAGCACATCAGGGAGTGGCTGTTCTGGGGATTGCCCTTATT GCTATGGGGGAGGAGATTGGTGCAGAGATGGCATTACGAACCTTTGGCCACTTGCTGAGA TATGGGGAGCCTACACTCCGGAGGGCTGTACCTTTAGCACTGGCCCTCATCTCTGTTTCA AATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCTCTCATGATGCTGATCCAGAA GTTTCCTATAACTCCATTTTTGCCATGGGCATGGTGGGCAGTGGTACCAATAATGCCCGT CTGGCTGCAATGCTGCGCCAGTTAGCTCAATATCATGCCAAGGACCCAAACAACCTCTTC ATGGTGCGCTTGGCACAGGGCCTGACACATTTAGGGAAGGGCACCCTTACCCTCTGCCCC TACCACAGCGACCGGCAGCTTATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTT GTCTCTTTCCTGGATGTTCGAAACATTATTCTAGGCAAATCACACTATGTATTGTATGGG CTGGTGGCTGCCATGCAGCCCCGAATGCTGGTTACGTTTGATGAGGAGCTGCGGCCATTG CCAGTGTCTGTCCGTGTGGGCCAGGCAGTGGATGTGGTGGGCCAGGCTGGCAAGCCGAAG ACTATCACAGGGTTCCAGACGCATACAACCCCAGTGTTGTTGGCCCACGGGGAACGGGCA GAATTGGCCACTGAGGAGTTTCTTCCTGTTACCCCCATTCTGGAAGGTTTTGTTATCCTT CGGAAGAACCCCAATTATGATCTCTAA Clone variation with respect to NM_002808.3 57 g=>n;58 g=>n;59 c=>n;60 c=>n >OriGene 5' read for NM_002808 unedited
TGCACCATTTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGCGGAGA TGGAGGAGGGAGGCCGGGACAAGGCGCCGGTGCAGCCCCAGCAGTCTCCAGCGGCGGCCC CCGGCGGCACGGACGAGAAGCCGAGCGGCAAGGAGCGGCGGGATGCCGGGGACAAGGACA AAGAACAGGAGCTGTCTGAAGAGGATAAACAGCTTCAAGATGAACTGGAGATGCTCGTGG AACGACTAGGGGAGAAGGATACATCCCTGTATCGACCAGCGCTGGAGGAATTGCGAAGGC AGATTCGTTCTTCTACAACTTCCATGACTTCAGTGCCCAAGCCTCTCAAATTTCTGCGTC CACACTATGGCAAACTGAAGGAAATCTATGAGAACATGGCCCCTGGGGAGAATAAGCGTT TTGCTGCTGACATCATCTCCGTTTTGGCCATGACCATGAGTGGGGAGCGTGAGTGCCTCA AGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCATGGGGTCATGAGTATGTCAGGC ATCTGGCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGAGAAGGTCCAGC GGGAGCCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCCACATGCAG AGCATGAGGCTTGCGACCTGCTTATGGAAATTGAGCANGTGGACATGCTGNAGAAGGACA TTGATGAANATGCATATGCAAAGGTCTGCCTTTATCTCACCAGNTGTGTGAANTACGTGC CTGAGCCTGAGAACTCAGCCCTACTGCGTTGNGCCCTGGGTGTGTTTCGAAAGTTTAGCC GCTTCCCTGAACCTCTGAGATTGGCATTGATGCTCAATGACATGGNAGTGGCAGAAGACA TCTTCACCTCCTGCAGGCATTGTGCAGTACAAN >OriGene 3' read for NM_002808 unedited
CAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTCCTTGGGGATAAAAGTCTTTATTGAAC AACCTTATCTCACTCAGTAACAAAAGAGCAGGAGGCGACAATTCCCCCAGAAGTCTGCAG CCGTGTCCACCCTTGGCAGCAGGATGCATGGCCTGCTGATAACATCAGCTGCAGTTCAGA GCCCCTGGTGGCCACTTAGAGATCATAATTGGGGTTCTTCCGAAGGATAACAAAACCTTC CAGATTGGGGGTAACAGGAAGAAACTCCTCAGTGGCCAATTCTGCCCGTTCCCCGTGGGC CAACAACACTGGGGTTGTATGCGTCTGGAACCCTGTGATAGTCTTCGGCTTGCCAGCCTG GCCCACCACATCCACTGCCTGGCCCACACGGACAGACACTGGCAATGGCCGCAGCTCCTC ATCAAACGTAACCAGCATTCGGGGCTGCATGGCAGCCACCAGCCCATACAATACATAGTG TGATTTGCCTAGAATAATGTTTCGAACATCCAGGAAAGATACAAGCACAGTGAGCAGTCC AGCCACGGCCACCTGGCTCATAAGCTGCCGGCCGCTGTGGTATGGGCAGAGGGAAAGGGT GCCCCTTCCCTAATGTGTCAGGCCCTGTGCCAAGCCCACCATGAAGAGGTCTGTTTGGTC CCTAGCCATGATATTGAGCTAACTGGCGCAGCATTGCAGCCAGACGGGCTTTTTTGGGGC CACTGCCCACCTGCCCATGGCAAAATGGAGTTTTAGGATACTTCGGATCAGCATCATGAG AGAATTTGCTAAGGGTCTCCAGGATGTCGAGTTCGTGGACTGAAACAGAGATGAGGGCCA CTGCTAAGGTACCACCCTCCGAATGTAAGCCCCCCATATCCTACAAGGGGCCAGGTTCTG AAAGGCCTTTTGACCCATTTCCCCCNCTTACATATAGGCAATCCCAGAACACACCTCCTG GGGGCCCCCTGTAAAAGGGCTCCTTATGGCCTGTTCTTCTTACTCG |
Restriction Sites | NotI-NotI |
ACCN | NM_002808 |
Insert Size | 3000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002808.3, NP_002799.3 |
RefSeq Size | 2990 bp |
RefSeq ORF | 2727 bp |
Locus ID | 5708 |
UniProt ID | Q13200 |
Domains | PC_rep |
Protein Families | Druggable Genome |
Protein Pathways | Proteasome |
Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203204 | PSMD2 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2) |
CNY 6,648.00 |
|
RC203204L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2), Myc-DDK-tagged |
CNY 9,048.00 |
|
RC203204L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2), mGFP tagged |
CNY 11,500.00 |
|
RG203204 | PSMD2 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2) |
CNY 8,248.00 |
|
SC320769 | PSMD2 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2) |
CNY 6,656.00 |