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PGAM1 (NM_002629) Human Untagged Clone
CAT＃: SC118510
PGAM1 (untagged)-Human phosphoglycerate mutase 1 (brain) (PGAM1)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-S-35; PGAM-B; PGAMA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118510 sequence for NM_002629 edited (data generated by NextGen Sequencing)
ATGGCCGCCTACAAACTGGTGCTGATCCGGCACGGCGAGAGCGCATGGAACCTGGAGAAC CGCTTCAGCGGCTGGTACGACGCCGACCTGAGCCCGGCGGGCCACGAGGAGGCGAAGCGC GGCGGGCAGGCGCTACGAGATGCTGGCTATGAGTTTGACATCTGCTTCACCTCAGTGCAG AAGAGAGCGATCCGGACCCTCTGGACAGTGCTAGATGCCATTGATCAGATGTGGCTGCCA GTGGTGAGGACTTGGCGCCTCAATGAGCGGCACTATGGGGGTCTAACCGGTCTCAATAAA GCAGAAACTGCTGCAAAGCATGGTGAGGCCCAGGTGAAGATCTGGAGGCGCTCCTATGAT GTCCCACCACCTCCGATGGAGCCCGACCATCCTTTCTACAGCAACATCAGTAAGGATCGC AGGTATGCAGACCTCACAGAAGATCAGCTACCCTCCTGTGAGAGTCTGAAGGATACTATT GCCAGAGCTCTGCCCTTCTGGAATGAAGAAATAGTTCCCCAGATCAAGGAGGGGAAACGT GTACTGATTGCAGCCCATGGCAACAGCCTCCGGGGCATTGTCAAGCATCTGGAGGGTCTC TCTGAAGAGGCTATCATGGAGCTGAACCTGCCGACTGGTATTCCCATTGTCTATGAATTG GACAAGAACTTGAAGCCTATCAAGCCCATGCAGTTTCTGGGGGATGAAGAGACGGTGCGC AAAGCCATGGAAGCTGTGGCTGCCCAGGGCAAGGCCAAGAAGTGA Clone variation with respect to NM_002629.2 >OriGene 5' read for NM_002629 unedited
NGTCAGATATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCATCC CCAGCCCGCCGCCATGGCCGCCTACAAACTGGTGCTGATCCGGCACGGCGAGAGCGCATG GAACCTGGAGAACCGCTTCAGCGGCTGGTACGACGCCGACCTGAGCCCGGCGGGCCACGA GGAGGCGAAGCGCGGCGGGCAGGCGCTACGAGATGCTGGCTATGAGTTTGACATCTGCTT CACCTCAGTGCAGAAGAGAGCGATCCGGACCCTCTGGACAGTGCTAGATGCCATTGATCA GATGTGGCTGCCAGTGGTGAGGACTTGGCGCCTCAATGAGCGGCACTATGGGGGTCTAAC CGGTCTCAATAAAGCAGAAACTGCTGCAAAGCATGGTGAGGCCCAGGTGAAGATCTGGAG GCGCTCCTATGATGTCCCACCACCTCCGATGGAGCCCGACCATCCTTTCTACAGCAACAT CAGTAAGGATCGCAGGTATGCAGACCTCACAGAAGATCAGCTACCCTCCTGTGAGAGTCT GAAGGATACTATTGCCAGAGCTCTGCCCTTCTGGAATGAAGAAATAGTTCCCCAGATCAA GGAGGGNGAAACGTGTACTGATTGCAGCCCATGGCAACAGCCTCCGGGGCATTGTCAAGC ATCTGGGAGGGTCTCTCTGAAGAGGCTATCATGGAGCTGAACCTGCCGACTGGTATTCCC ATTGTCTATGAATTGGACAAGAACTTGAAGCCTATCAAGCCCATGCAGTTTCTGGGGGAT GAAGAGACGGTGCGCAAAGCCTGGGAGCTGTGGCTGCCCAGGCAGGCCAAGAAGTGAGGC CGGCGGGAGGATACTGTCCCAGAAGCAACCTCCTGCCCGTTTGGTCCTCTGCCCTCCACC TGCCATGTACACTGACACATCTGTAACTTTTTGAGTGTACTGCNACGGGGACAATGGCTT CATT >OriGene 3' read for NM_002629 unedited
ATGGCACTTCCAGGGCCGGNATAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGGATC TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT TTATTGCACATCACTCCTTTATTATACTGATATGGAAAAAGGATTTAGTACAGTTATGCT CAGATGAACACTGGACCCATGTGGCAGGGTCAAGCAACTAGAACATGATTCAGAAATCAG TGAAAGATACACTTGGACAGGACCAAGAGGCATTTCACTGCCATGAAACAAGGCAGGAAG GGATTCTAATACACACACCAGGAAGCACTCCTGCCCCTCAGAGGTCAAGGAGCTGATCCT ATATTGGTATGAGGAATGGCTTATTTTCTGATGACCACATGTGGGACTATTTCAACCGCC ACAAGAAACCCCAGAAGGGTTATTGTTTTGTATTATATATACTATACTTTTTTAATAAAA GTAAATTAACACATAACGAAATTCAGGATTGATCCCAACCTAGAGCCAGATCCTCTGGGG TCAGGGAGGAAACAGTTGTCACATCACCACGCAGGTTACATTCGTCTTCCACTGGAATGA CTAGAGCCCCCAGGCAGTAGGCAGTGACCTGACTGCAGAAGAGCAGAGGACAGACTCCGC TCATGGGGACAGACAGGCTCTGTTGCTTCTCCTCACTGGTCATGGCTTANCATGGTTCCT CCCCAAGTCCTTAGTAAACAAAGCACTCGCANAAACCCAAGTCACTACTTTTAAACTCTG TTGGATAAGGGGAGCTTTTCCATAGCTAGACTGAGAACCTGTGCTCTAGAACTGCTATTC TGACTANATGTATGAAAGGAAGTGGGTGCANGCGACAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002629 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002629.2, NP_002620.1 |
| RefSeq Size | 1762 bp |
| RefSeq ORF | 765 bp |
| Locus ID | 5223 |
| UniProt ID | P18669 |
| Domains | PGAM |
| Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways |
| Gene Summary | The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204054 | PGAM1 (Myc-DDK-tagged)-Human phosphoglycerate mutase 1 (brain) (PGAM1) |
CNY 3,600.00 |
|
| RC204054L1 | Lenti ORF clone of Human phosphoglycerate mutase 1 (brain) (PGAM1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC204054L2 | Lenti ORF clone of Human phosphoglycerate mutase 1 (brain) (PGAM1), mGFP tagged |
CNY 6,000.00 |
|
| RC204054L3 | Lenti ORF clone of Human phosphoglycerate mutase 1 (brain) (PGAM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204054L4 | Lenti ORF clone of Human phosphoglycerate mutase 1 (brain) (PGAM1), mGFP tagged |
CNY 6,000.00 |
|
| RG204054 | PGAM1 (tGFP-tagged) - Human phosphoglycerate mutase 1 (brain) (PGAM1) |
CNY 5,200.00 |
|
| SC324561 | PGAM1 (untagged)-Human phosphoglycerate mutase 1 (brain) (PGAM1) |
CNY 3,600.00 |
