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PDK2 (NM_002611) Human Untagged Clone
CAT＃: SC118540
PDK2 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 2 (PDK2), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PDHK2; PDKII |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_002611 edited
ATGCGCTGGGTGTGGGCGCTGCTGAAGAATGCGTCCCTGGCAGGGGCGCCCAAGTACATA GAGCACTTCAGCAAGTTCTCCCCGTCCCCGCTGTCCATGAAGCAGTTTCTGGACTTCGGA TCCAGCAATGCCTGTGAGAAAACCTCCTTCACCTTCCTCAGGCAGGAGCTGCCTGTGCGC CTGGCCAACATCATGAAAGAGATCAACCTGCTTCCCGACCGAGTGCTGAGCACACCCTCC GTGCAGCTGGTGCAGAGCTGGTATGTCCAGAGCCTCCTGGACATCATGGAGTTCCTGGAC AAGGATCCCGAGGACCATCGCACCCTGAGCCAGTTCACTGACGCCCTGGTCACCATCCGG AACCGGCACAACGACGTGGTGCCCACCATGGCACAAGGCGTGCTTGAGTACAAGGACACC TACGGCGATGACCCCGTCTCCAACCAGAACATCCAGTACTTCCTGGACCGCTTCTACCTC AGCCGCATCTCCATCCGCATGCTCATCAACCAGCACACCCTCATCTTTGATGGCAGCACC AACCCAGCCCATCCCAAACACATCGGCAGCATCGACCCCAACTGCAACGTCTCTGAGGTG GTCAAAGATGCCTACGACATGGCTAAGCTCCTGTGTGACAAGTATTACATGGCCTCACCT GACCTGGAGATCCAGGAGATCAATGCAGCCAACTCCAAACAGCCGATTCACATGGTCTAC GTCCCCTCCCACCTCTACCACATGCTCTTTGAGCTCTTCAAGAATGCCATGAGGGCGACT GTGGAAAGCCATGAGTCCAGCCTCATTCTCCCACCCATCAAGGTCATGGTGGCCTTGGGT GAGGAAGATCTGTCCATCAAGATGAGTGACCGAGGTGGGGGTGTTCCCTTGAGGAAGATT GAGCGACTCTTCAGCTACATGTACTCCACAGCACCCACCCCCCAGCCTGGCACCGGGGGA ACGCCGCTGGCTGGCTTTGGTTATGGGCTCCCCATTTCCCGCCTCTACGCCAAGTACTTC CAGGGAGACCTGCAGCTCTTCTCCATGGAAGGCTTTGGGACCGATGCTGTCATCTATCTC AAGGCCCTGTCCACGGACTCGGTGGAGCGCCTGCCTGTCTACAACAAGTCAGCCTGGCGC CACTACCAGACCATCCAGGAGGCCGGCGACTGGTGTGTGCCCAGCACGGAGCCCAAGAAC ACGTCCACGTACCGCGTCAGCTAA >OriGene 5' read for NM_002611 unedited
GCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGCAGCCATGCGCT GGGTGTGGGCGCTGCTGAAGATGCGTCCCTGGCAGGGGCGCCCAAGTACATAGAGCACTT CAGCAAGTTCTCCCCGTCCCCGCTGTCCATGAAGCAGTTTCTGGACTTCGGATCCAGCAA TGCCTGTGAGAAAACCTCCTTCACCTTCCTCAGGCAGGAGCTGCCTGTGCGCCTGGCCAA CATCATGAAAGAGATCAACCTGCTTCCCGACCGAGTGCTGAGCACACCCTCCGTGCAGCT GGTGCAGAGCTGGTATGTCCAGAGCCTCCTGGACATCATGGAGTTCCTGGACAAGGATCC CGAGGACCATCGCACCCTGAGCCAGTTCACTGACGCCCTGGTCACCATCCGGAACCGGCA CAACGACGTGGTGCCCACCATGGCACAAGGCGTGCTTGAGTACAAGGACACCTACGGCGA TGACCCCGTCTCCAACCAGAACATCCAGTACTTCCTGGACCGCTTCTACCTCAGCCGCAT CTCCATCCGCATGCTCATCAACCAGCACACCCTCATCTTTGATGGCAGCACCAACCCAGC CCATCCCAAACACATCGGCAGCATCGACCCCAACTGCAACGTCTCTGAGGTGGTCAAAGA TGCCTACGACATGGCTAAGCTCCTGTGTGACAAGTATTACATGGCCTCACCTGACCTGNA GATCCAGGAGATCAATGCAGCCAACTCCAAACAGCCGATTCACATGGTCTACGTCCCCCT CCACCTCTACCACATGCTCTTTGAGCTCTTCAGAATGCCCTGAGGGCGACTGTGGAAAGC CATGAGTCCAGCCTCATTCTCCCACCATCAAGTCATGGTGCCCTTGGTGAGGAAAGACTG TCCATCAGATATGACGAGGGNNGGGTGGTCCTTGAGGAGATGACCCATCTCACCTN >OriGene 3' read for NM_002611 unedited
TATGAACCGCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTCTCATTCAGT CTCCTCTTTACTGTGGGTGTGGGTGTCACTGTCACTGCCACAGCCACTGGGAGGGACACA CAGCTTTAACCCCTGTGTGCTTAGGGGAAGGGTGGGGGCATTCAGGGTTATAAAACTAAC TATATACACAGAAGGTCCTAGGGAGAAAGCCACCCTGAGCACACATGTCTGGGCACAGTG GGGGCTGGGGGCTGGAGCTCAGGCAGGATGGACTAGGCTTGTGGAGGATCGGGTGGGCAT GAGCATGTGAGGACATGCTGGGAGGGCTCAGGAGGTGGCACAGACATTGCCAAGGCCACT GCAGGGCCTCGAGCAGCTTGGCTGGATGGCAAGCTCTCGGTCTAACAAGGTTCTTCCACT TTAGAGTTGGGCCCTGTGAGAGCCACCCCCTCCGCCACCGACCTTCAAGCCCTGAGCGGA GGGAGAGGCTGCAGGACCAGCCTCATTCCCCACTCACGGGTGTAACTCCTTTCCCCACTC AGTCCCCAGGTCTGGAGGGCACCCACCCTCTTCCTAACACTTCAGCAGACCAAGGCCAGG GTGCCTTTGTGGGCTGGGGGAATAAGGGCACGGGACATCCACTTGGAAGGCCTGTGCTGT GGAGGGTGCTGAGGGGGACCCAGTACCCCTGGCAGAAAAGTGACAGCCCTGGTCTCAGAG AGGAGGACTGCCATGGGATAATGTCTCTGGGACACCCCATCAGGGTGGAGACATCCCTAA CCCTCAGGCATTCGCCACAGAGATAAGGNAGCCACCNGAGGGTCTNCCANAAGAGGACTG CCCTTAGGGACCTCACAGACCTTCACCGCAATGACTCCATATAACAAAACCTGGCATCAG GAAAACCCACCCCCTGCTCCCCAGTAGATGGTGAGGGCACCACGTGGGGGCACCAAGCGG CATTCCGTCTTTTAGGGCAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002611 |
| Insert Size | 2320 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002611.3, NP_002602.2 |
| RefSeq Size | 2319 bp |
| RefSeq ORF | 1224 bp |
| Locus ID | 5164 |
| UniProt ID | Q15119 |
| Domains | HATPase_c |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
p53 Negatively Regulates Transcription of the Pyruvate Dehydrogenase Kinase Pdk2
,Tanupriya Contractor and Chris R. Harris,
Cancer Res., Jan 2012; 72: 560 - 567.
[PDK2]
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| RC205937L2 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 2 (PDK2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
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| RC205937L3 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 2 (PDK2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
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| RC205937L4 | Lenti ORF clone of Human pyruvate dehydrogenase kinase, isozyme 2 (PDK2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
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| RG205937 | PDK2 (tGFP-tagged) - Human pyruvate dehydrogenase kinase, isozyme 2 (PDK2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 7,088.00 |
