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NRAS (NM_002524) Human Untagged Clone
CAT＃: SC118574
NRAS (untagged)-Human neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS)
CNY 3,600.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALPS4; CMNS; N-ras; NCMS; NRAS1; NS6 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002524 edited
GAATTCGGCACGAGGCCTTGGTGGGGGCTGTTCATGGCGGTTCCGGGGTCTCCAACATTT TTCCCGGCTGTGGTCCTAAATCTGTCCAAAGCAGAGGCAGTGGAGCTTGAGGTTCTTGCT GGTGTGAAATGACTGAGTACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCG CACTGACAATCCAGCTAATCCAGAACCACTTTGTAGATGAATATGATCCCACCATAGAGG ATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACCTGTTTGTTGGACATACTGGATA CAGCTGGACAAGAAGAGTACAGTGCCATGAGAGACCAATACATGAGGACAGGCGAAGGCT TCCTCTGTGTATTTGCCATCAATAATAGCAAGTCATTTGCGGATATTAACCTCTACAGGG AGCAGATTAAGCGAGTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGT GTGATTTGCCAACAAGGACAGTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACG GGATTCCATTCATTGAAACCTCAGCCAAGACCAGACAGGGTGTTGAAGATGCTTTTTACA CACTGGTAAGAGAAATACGCCAGTACCGAATGAAAAAACTCAACAGCAGTGATGATGGGA CTCAGGGTTGTATGGGATTGCCATGTGTGGTGATGTAACAAGATACTTTTAAAGTTTTGT CAGAAAAGAGCCACTTTCAAGCTGCACTGACACCCTGGTCCTGACTTCCCTGGAGGAGAA GTATTCCTGTTGCTGTCTTCAGTCTCACAGAGAAGCTCCTGCTACTTCCCCAGCTCTCAG TAGTTTAGTACAATAATCTCTATTTGAGAAGTTCTCAGAATAACTACCTCCTCACTTGGC TGTCTGACCAGAGAATGCACCTCTTGTTACTCCCTGTTATTTTTCTGCCCTGGGTTCTTC CACAGCACAAACACACCTCTGCCACCCCAGGTTTTTCATCTGAAAAGCAGTTCATGTCTG AAACAGAGAACCAAACCGCAAACGTGAAATTCTATTGAAAACAGTGTCTTGAGCTCTAAA GTAGCAACTGCTGGTGATTTTTTTTTTCTTTTTACTGTTGAACTTAGAACTATGCTAATT TTTGGAGAAATGTCATAAATTACTGTTTTGCCAAGAATATAGTTATTATTGCTGTTTGGT TTGTTTATAATGTTATCGGCTCTATTCTCTAAACTGGCATCTGCTCTAGATTCATAAATA CAAAAATGAATACTGAATTTTGAGTCTATCCTAGTCTTCACAACTTTGACGTAATTAAAT CCAACTTTCACAGTGAAGTGCCTTTTTCCTAGAAGTGGTTTGTAGACTTCCTTTATAATA TTTCAGTGGAATAGATGTCTCAAAAATCCTTATGCATGAAATGAATGTCTGAGATACGTC TGTGACTTATCTACCATTGAAGGAAAGCTATATCTATTTGAGAGCAGATGCCATTTTGTA CATGTATGAAATTGGTTTTCCAGAGGCCTGTTTTGGGGCTTTCCCAGGAGAAAGATGAAA CTGAAAGCATATGAATAATTTCACTTAATAATTTTTACCTAATCTCCACTTTTTTCATAG GTTACTACCTATACAATGTATGTAATTTGTTTCCCCTAGCTTACTGATAAACCTAATATT CAATGAACTTCCATTTGTATTCAAATTTGTGTCATACCAGAAAGCTCTACATTTGCAGAT GTTCAAATATTGTAAAACTTTGGTGCATTGTTATTTAATAGCTGTGATCAGTGATTTTCA AACCTCAAATATAGTATATTAACAAATTAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_002524 unedited
TTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTGGTGGGGGCTG TTCATGGCGGTTCCGGGGTCTCCAACATTTTTCCCGGCTGTGGTCCTAAATCTGTCCAAA GCAGAGGCAGTGGAGCTTGAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGG TGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACAATCCAGCTAATCCAGAACCACT TTGTAGATGAATATGATCCCACCATAGAGGATTCTTACAGAAAACAAGTGGTTATAGATG GTGAAACCTGTTTGTTGGACATACTGGATACAGCTGGACAAGAAGAGTACAGTGCCATGA GAGACCAATACATGAGGACAGGCGAAGGCTTCCTCTGTGTATTTGCCATCAATAATAGCA AGTCATTTGCGGATATTAACCTCTACAGGGAGCAGATTAAGCGAGTAAAAGACTCGGATG ATGTACCTATGGTGCTAGTGGGAAACAAGTGTGATTTGCCAACAAGGACAGTTGATACAA AACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATTCATTGAAACCTCAGCCAAGA CCAGACAGGGTGTTGAAGATGCTTTTTACACACTGGTAAGAGAAATACGCCAGTACCGAA TGAAAAAACTCAACAGCAGTGATGATGGGACTCAGGNTTGTATGGGATTGCCATGTGTGG TGATGTAACAANGATACTTTAAAGTTTTGTCAGAAAAGAGCCACTTTTCAGCTGCACTGA CACCCTGGTCCTGACTTCCCTGGNAGAGAAGTATNCCTGTTGCTGTCTTCAGTCTCACAG AGAAGCTNCTGCTACTTNCCCACTCTNAGTAGTTNAGNACAATATCTCTATTTGAGAAGT CTCAAATAACTACCTCTCACTGGCT >OriGene 3' read for NM_002524 unedited
GTACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTGTTAATATA CTATATTTGAGGTTTGAAAATCACTGATCACAGCTATTAAATAACAATGCACCAAAGTTT TACAATATTTGAACATCTGCAAATGTAGAGCTTTCTGGTATGACACAAATTTGAATACAA ATGGAAGTTCATTGAATATTAGGTTTATCAGTAAGCTAGGGGAAACAAATTACATACATT GTATAGGTAGTAACCTATGAAAAAAGTGGAGATTAGGTAAAAATTATTAAGTGAAATTAT TCATATGCTTTCAGTTTCATCTTTCTCCTGGGAAAGCCCCAAAACAGGCCTCTGGAAAAC CAATTTCATACATGTACAAAATGGCATCTGCTCTCAAATAGATATAGCTTTCCTTCAATG GTAGATAAGTCACAGACGTATCTCAGACATTCATTTCATGCATAAGGATTTTTGAGACAT CTATTCCACTGAAATATTATAAAGGAAGTCTACAAACCACTTCTAGGAAAAAGGCACTTC ACTGTGAAAGTTGGATTTAATTACGTCAAAGTTGTGAAGACTAGGATAGACTCAAAATTC AGTATTCATTTTTGTATTTATGAATCTAGAGCAGATGCCAGTTTAGAGAATAGAGCCGAT AACATTATAAACAAACCAAACAGCACTAATAACTATATTTCTTGGCAAAACAGTAATTTA TGACATTTCTCCAAAAATAGCATAGTTCTAAGTTCAACAGTAAAAAGAAAAAAAAATCAC CACCAGTTGCTACTTTAGAGCTCAAGAACTGTTTTCATAGAATTCACGTTTGCGGTTTGG TTCTCTGTTCAGACATGACTGCTTTCAGATGAAAACCTGGGTGGCANAAGTTGTGTTGTG CTGTGGAAAACCCAGGCCAAATAATACAGGGGTTACAGGAGGGCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002524 |
| Insert Size | 1860 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002524.2, NP_002515.1 |
| RefSeq Size | 1963 bp |
| RefSeq ORF | 570 bp |
| Locus ID | 4893 |
| UniProt ID | P01111 |
| Domains | ras, RAS, RHO, RAB |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway |
| Gene Summary | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PNAS Plus: Lung cancers with acquired resistance to EGFR inhibitors occasionally harbor BRAF gene mutations but lack mutations in KRAS, NRAS, or MEK1
,Kadoaki Ohashi, Lecia V. Sequist, Maria E. Arcila, Teresa Moran, Juliann Chmielecki, Ya-Lun Lin, Yumei Pan, Lu Wang, Elisa de Stanchina, Kazuhiko Shien, Keisuke Aoe, Shinichi Toyooka, Katsuyuki Kiura, Lynnette Fernandez-Cuesta, Panos Fidias, James Chih-Hsin Yang, Vincent A. Miller, Gregory J. Riely, Mark G. Kris, Jeffrey A. Engelman, Cindy L. Vnencak-Jones, Dora Dias-Santagata, Marc Ladanyi, and William Pao,
PNAS, Jul 2012; 109: E2127 - E2133.
[NRAS]
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Resources
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