NFYA (NM_002505) Human Untagged Clone
CAT#: SC118595
NFYA (untagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1
CNY 3,656.00
CNY 5,610.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CBF-A; CBF-B; HAP2; NF-YA |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC118595 sequence for NM_002505 edited (data generated by NextGen Sequencing)
ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGA CAGATTCAGCAGCAGCAGCAGGGTGGTGTCACTGCTGTGCAGTTGCAGACTGAGGCCCAG GTGGCATCCGCCTCAGGCCAGCAAGTCCAGACCCTCCAGGTAGTCCAAGGGCAGCCATTA ATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACCCATCATGGTCCAG GCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAGGGT TTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTG CAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCT GTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCA CAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGGCACCATTCTC CAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGCCAGTTTGGCAGGA GCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGGGCAAGGGACTGTC ACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGATGGTT CCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGATGCTT GAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAGGCAA GCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCTGCAT GAGTCTCGGCACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGGACGATTTTTCTCT CCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAAACCAAGCCGATGAAGAAGCAATG ACACAGATCATCCGAGTGTCCTAA Clone variation with respect to NM_002505.4 >OriGene 5' read for NM_002505 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCG GCACGAGGCGGCGGCAGCGGCGGCTGGAGCCTCTGATTGGGTTTCGGAGTCCGGTACTGG AGCCAATCAGCGCGGGCAGCGAACCGGGGGAGCGAGGCACGGAGTGTACCTCACAGCCTT CTAGGATCTCCAGAGTGGACAGGAATCTCACTTGGAGGGACCATGGAGCAGTATACAGCA AACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGCAGCAGCAG CAGGGTGGTGTCACTGCTGTGCAGTTGCAGACTGAGGCCCAGGTGGCATCCGCCTCAGGC CAGCAAGTCCAGACCCTCCAGGTAGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGA GGCCAGCTAATCACATCAACTGGCCAACCCATCATGGTCCAGGCTGTCCCTGGTGGACAA GGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAGGGTTTGCAGCAAATACAGTTG GTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTGCAGGTGCAGGGCCAGCAG GGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCTGTCACTGCTGGCCAGACT CAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTGAAGGGCAG ACCATCGTCTATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAGTTACAGTCCCTG GTTCAGGCATGATCACTATCCAGCAGCCAGTTTGGGCAGAGCCCAGATTGTNAAACAGGA GCCATACCACACACCAGCAGTGGCAAGGGACTGTACTGTGCACTACAGTG |
Restriction Sites | NotI-NotI |
ACCN | NM_002505 |
Insert Size | 3800 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002505.3, NP_002496.1 |
RefSeq Size | 2834 bp |
RefSeq ORF | 1044 bp |
Locus ID | 4800 |
UniProt ID | P23511 |
Domains | CBF |
Protein Families | Transcription Factors |
Protein Pathways | Antigen processing and presentation |
Gene Summary | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) includes alternate exon B, resulting in the long isoform, isoform 1. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Radiation and Cisplatin activation of EBV in nasopharyngeal cancer: Understanding the oncolytic potential of EBV
,Marie C. Chia, Abby Leung, Tiffaney Krushel, Nehad Alajez, Carlo Bastianutto and Fei-Fei Liu,
Proc Amer Assoc Cancer Res, Volume 47, 2006; Abstract #823
[NFYA]
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