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MCM3 (NM_002388) Human Untagged Clone
CAT＃: SC118658
MCM3 (untagged)-Human minichromosome maintenance complex component 3 (MCM3)
CNY 8,888.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HCC5; P1-MCM3; P1.h; RLFB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002388, the custom clone sequence may differ by one or more nucleotides
ATGCTCCCAAGGTCCCCGCCTCTTCCTCGAGGTAACCTTTGGTGGCGGGAAGAGTTCGGAAGTTTTCGCG CCGGGGTGGAGTCATCCTGGGAACCTCCACGCGACTTTGGTGGAGGTAGTTCTTTGGCAGCGGGCATGGC GGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGGACGAC GAGGAAGACCAGGGAATTTATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCTGATTG TCAATGTGAATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTTGAGGA GCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTATGCCAAGCAGTAT GAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGGCTCCAAGCACGTCTCCCCGCGGACTCTTACCTCCT GCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTCACTAAATGTTCTCTAGTTCGTCCCAAAGTCGT CCGCAGTGTCCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTG GTGGCCTTTCCCTCCAGCTCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCCTTGAGACAGAATATG GCCTTTCTGTCTACAAGGATCACCAGACCATCACCATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCA GCTCCCCCGCTCTGTGGACGTCATTCTGGATGATGACTTGGTGGATAAAGCGAAGCCTGGTGACCGGGTT CAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAAGAAGGGAGGCTACACCTCTGGGACCTTCAGGACTG TCCTGATTGCCTGTAATGTTAAGCAGATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGC CAAGATCAAGAAGTTCAGTAAAACCCGATCCAAGGATATCTTTGACCAGCTGGCCAAGTCATTGGCCCCA AGTATCCATGGGCATGACTATGTCAAGAAAGCAATCCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACC TAGAAAATGGCAGCCACATCCGTGGGGACATCAATATTCTTCTAATAGGAGACCCATCCGTTGCCAAGTC TCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCCGAGCTATCCCCACCACTGGCCGGGGCTCCTCTGGA GTGGGTCTGACGGCTGCTGTCACCACAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGG TCCTGGCTGACCGAGGCGTGGTTTGCATTGATGAATTTGACAAAATGTCTGACATGGATCGCACAGCCAT CCATGAAGTGATGGAGCAGGGTCGAGTGACCATTGCCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGC TGCAGTGTTTTGGCAGCTGCCAACCCTGTCTACGGCAGGTATGACCAGTATAAGACTCCAATGGAGAACA TTGGGCTACAGGACTCACTGCTGTCACGATTTGACTTGCTCTTCATCATGCTGGATCAGATGGATCCTGA GCAGGATCGGGAGATCTCAGACCATGTCCTTCGGATGCACCGTTACAGAGCACCTGGGGAGCAGGATGGC GATGCTATGCCCTTGGGTAGTGCTGTGGATATCCTGGCCACAGATGATCCCAACTTTAGCCAGGAAGATC AGCAGGACACCCAGATTTATGAGAAGCATGACAACCTTCTACATGGGACCAAGAAGAAAAAGGAGAAGAT GGTGAGTGCAGCATTCATGAAGAAGTACATCCATGTGGCCAAAATCATCAAGCCTGTCCTGACACAGGAG TCGGCCACCTACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAGGATAGCATGAGCTCAGACACCGCCA GGACATCTCCAGTTACAGCCCGAACACTGGAAACTCTGATTCGACTGGCCACAGCCCATGCGAAGGCCCG CATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCTGTGGAGTTGGTCCAGTATGCTTACTTTAAG AAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCGAAGTGAGGATGAATCAGAGACAGAAGATGAAGAGG AGAAAAGCCAAGAGGACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAAGATGG GGATTCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAATGCCTCAAGTACACACTCCAAAGACG GCAGACTCACAGGAGACCAAGGAATCCCAGAAAGTGGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGG TGGCCCTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTCAATCGGCATGAATCGCCTCACAGAATCCAT CAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTGAGATCCAGGCTGCTCTGAGCAAGATGCAGGATGAC AATCAGGTCATGGTGTCTGAGGGCATCATCTTCCTCATCTGA >OriGene 5' read for NM_002388 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCACGCGACTTT GGTGGAGGTAGTTCTTTGGCAGCGGGCATGGCGGGTACCGTGGTGCTGGACGATGTGGAG CTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGGACGACGAGGAAGACCAGGGAATT TATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCTGATTGTCAATGTG AATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTTGAG GAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTAT GCCAAGCAGTATGAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGGCTCCAAGCACGTC TCCCCGCGGACTCTTACCTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTC ACTAAATGTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCTACT AAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGCCTTTCCCTCCAGC TCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCCTTGAGACAGAATATGGCCTTTCT GTCTACAAGGATCACCAGACCATCACCATCCAGGAGATGCCGGAGAAGGGCCCCAGCCGG CCAGCTCCCCCGCTCTGTGGACGTCATTCTGGATGATGACTTGGTGGATAAGCGAACCTG GTGACCGGTTCAGGTGTGGGGAACTACCGGTGNCTTCTGGAAGAGGGAGGCTACACTNTG GGACTTTCAGACTGGCCTGATTGCTGTAATGGTACCAA >OriGene 3' read for NM_002388 unedited
CTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGTACGAAATGGC TAAGTTTATTCAACACTCTCGGAATTCATCTGGATATTGGGTTTGTTTTGTGATACAATA CATATTCACCTTAACTGGTGCTACTGCAAAGAAAGCTTTCTTGACCTGCATGACGTGCCT CAGAGCTTCTCTCCACCAATTGGAACCACCCAAAGCCTAGTCTAGACCAAAGTGCTCTGG AGAAAAAAAACAAAACAAAAAAACAGCAAACAGAAAACAGTTGTGCCCCCAAAAGTACTC AGAAGTCATATGTTATTTACAATTGGGTTTGTGTGGGATGGGAAGTAGGGCGGATGAGCC AGTGCTTTTGCAATGAAGATGCAATAGTCATTGTCCTCTCCCACTGTCTCCTCTTTCCTC ACCCCATGGCAGCTTTCATGACCCATTCCCAAAGGGTCCACCGAGTCCTGAACTCAGCTT CATCACCAACATTCCTCGCCTTCAGTTGAATTCAACACTGTTAAGGGAGTAGAGGCAAAG ACTTGGGTCAGGGAGAGGGTGGGAAACACAGAACAAACTCTCTCGGCACAACCCAAGTTC AGAGACGAGGCCTCCTCAGATGAGGAAGATGATGCCCTCAGACACCATGACCTGATTGTC ATCCTGCATCTTGCTCAGAGCAGCCTGGATCTCAACTGAAGAGAANGGGCTCTCGCTGTC CCGGNTGATGGGATCTGTGAGGCGATTCATGCCGATTGACTGCGCATGAGCTTCCCGGAA CACATNNCAGAGGGCACCTTGNATGCCNTCACCTGNATCACTCACTCCACTTTCTGGATN NCTGGNCTCTGTGATCTGCCGCTTTGGATGTGTACTGAGCATTNCTCCTTGNGTACTGAG TCTAAGGTCGATGATCCCATCTTGCATCTGCTGGAGGCTNCTTCCTTCTTTTGGTCTGCC CCTGGCTTTCTCTTAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002388 |
| Insert Size | 2840 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002388.3, NP_002379.2 |
| RefSeq Size | 3143 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 4172 |
| UniProt ID | P25205 |
| Domains | MCM, AAA |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Cell cycle, DNA replication |
| Gene Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200208 | MCM3 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 3 (MCM3) |
CNY 8,872.00 |
|
| RC200208L3 | Lenti ORF clone of Human minichromosome maintenance complex component 3 (MCM3), Myc-DDK-tagged |
CNY 8,840.00 |
|
| RC200208L4 | Lenti ORF clone of Human minichromosome maintenance complex component 3 (MCM3), mGFP tagged |
CNY 8,840.00 |
|
| RG200208 | MCM3 (tGFP-tagged) - Human minichromosome maintenance complex component 3 (MCM3) |
CNY 7,600.00 |
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